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Abundance and Community Composition of Ammonia-Oxidizers in Paddy Soil at Different Nitrogen Fertilizer Rates

journal of Integrative Agriculture 2014年第4期13卷 870-880页

作者： SONG Ya-na LIN Zhi-min Institute of Biological Technology Fujian Academy of Agricultural Sciences

Ammonia oxidation, the first and rate-limiting step of nitrification, is carried out by both ammonia-oxidizing bacteria （AOB） and ammonia-oxidizing archaea （AOA）. However, the relative importance of AOB and AOA to nitrification in terrestrial ecosystems is not well understood. The aim of this study was to investigate the effect of the nitrogen input amount on abundance and community composition of AOB and AOA in red paddy soil. Soil samples of 10-20 cm （root layer soil） and 0-5 cm （surface soil） depths were taken from a red paddy. Rice in the paddy was fertilized with different rates of N as urea of N1 （75 kg N ha＂ yr-1）, N2 （150 kg N ha~ yrl）, N3 （225 kg N ha1 yrl） and CK （without fertilizers） in 2009, 2010 and 2011. Abundance and community composition of ammonia oxidizers was analyzed by real-time PCR and denaturing gradient gel electrophoresis （DGGE） based on amoA （the unit A of ammonia monooxygenase） gene. Archaeal amoA copies in N3 and N2 were significantly （P〈0.05） higher than those in CK and N1 in root layer soil or in surface soil under tillering and heading stages of rice, while the enhancement in bacterial amoA gene copies with increasing of N fertilizer rates only took on in root layer soil. N availability and soil NO3--N content increased but soil NH4＋-N content didn＇t change with increasing of N fertilizer rates. Otherwise, the copy numbers of archaeal amoA gene were higher （P〈0.05） than those of bacterial amoA gene in root lary soil or in surface soil. Redundancy discriminate analysis based on DGGE bands showed that there were no obvious differs in composition of AOA or AOB communities in the field among different N fertilizer rates. Results of this study suggested that the abundance of ammonia-oxidizers had active response to N fertilizer rates and the response of AOA was more obvious than that of AOB. Similarity in the community composition of AOA or AOB among different N fertilizer rates indicate that the community composition of ammonia-oxidizers w

关键词： ammonia-oxidizing bacteria ammonia-oxidizing archaea nitrogen fertilizer rates paddy soil

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Safety,immunogenicity,and preliminary efficacy of a randomized clinical trial ofomicron XBB.1.5-containing bivalent mRna vaccine

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hLife 2024年第3期2卷 113-125页

作者： Xuanjing Yu Wei Yang Wei Li na Wan Guanghong Yan Zumi Zhou Xiao Zhu Wei Su Yani Li Chenyu Xing Sifan Duan Houze Yu Xinshuai Zhao Chunmei Li Taicheng Zhou Dingyun You jia Wei Zijie Zhang State Key Laboratory for Conservation and Utilization of Bio-Resource and School of Life Sciences Yunnan UniversityYunnanChina Southwest United Graduate School YunnanChina State Key Laboratory of Genetic Engineering School of Life SciencesZhongshan HospitalFudan UniversityShanghaiChina Yunnan Vaccine Laboratory YunnanChina School of Public Health Kunming Medical UniversityYunnanChina Yunnan Genvoo Biotech Ltd. YunnanChina Central Lab and Liver Disease Research Center The Affiliated Hospital of Yunnan UniversityYunnan UniversityYunnanChina

Periodically updating coronavirus disease 19(COVID-19)vaccines that offer broad-spectrum protection is needed giventhe strong immune evasion by the circulating omicron *** effectiveness of prototype and BA.4/5-containing bivalent mRna vaccines is reduced when XBB subvariants *** initiated an observer-blinded,threearms study in 376 patients in Chinese individuals aged from 18 to 55 years old who had previously received three dosesCOVID-19 *** in terms of neutralizing antibodies elicited by a 30-mg dose of XBB.1.5-containingbivalent vaccine(RQ3027),a 30-mg dose of BA.2/BA.5-Alpha/Beta bivalent vaccine(RQ3025)and their precedent 30-mg Alpha/Beta(combined mutations)monovalent mRna vaccine(RQ3013)and safety are primary and secondary endpoints,*** recorded prescribed COVID-19 cases to explore the preliminary efficacy of three ***3027 and RQ3025 boosters elicited superior neutralizing antibodies(nabs)against XBB.1.5,XBB.1.16,XBB.1.9.1,and jN.1 compared to RQ3013 at day 14 in participants without SARS-CoV-2 *** study vaccines were welltolerated without serious adverse reactions *** incidence rates per 1000 person-years of COVID-19 casesduring the 2nd-19th week after randomization were lowest in ***,our data show that XBB.1.5-containingbivalent booster generated superior immunogenicity and better protection against newer severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)variants compared to BA.2/BA.5-containing bivalent and Alpha/Beta monovalentwith no new safety concerns.

关键词： severe acute respiratory syndrome coronavirus 2(SARS-CoV-2) jN.1 XBB lineages coronavirus disease19(COVID-19)vaccine mRna bivalent vaccine randomized clinical trial

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XELOX(capecitabine plus oxaliplatin)plus bevacizumab(anti-VEGF-A antibody)with or without adoptive cell immunotherapy in the treatment of patients with previously untreated metastatic colorectal cancer:a multicenter,open-label,randomized,controlled,phase 3 trial

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Signal Transduction and Targeted Therapy 2024年第5期9卷 2104-2113页

作者： Qiu-Zhong Pan jing-jing Zhao Liang Liu Dong-Sheng Zhang Li-Ping Wang Wen-Wei Hu De-Sheng Weng Xiang Xu Yi-Zhuo Li Yan Tang Wei-Hong Zhang jie-Yao Li Xiao Zheng Qi-jing Wang Yong-Qiang Li Tong Xiang Li Zhou Shuang-Ning Yang Chen Wu Rong-Xing Huang jia He Wei-jiao Du Lu-jun Chen Yue-na Wu Bin Xu Qiong Shen Yi Zhang jing-Ting jiang Xiu-Bao Ren jian-Chuan Xia State Key Laboratory of Oncology in South China Guangdong Provincial Clinical Research Center for CancerCollaborative Innovation Center for Cancer MedicineSun Yat-Sen University Cancer CenterGuangzhouGuangdong 510060PR China Department of Biotherapy Sun Yat-Sen University Cancer CenterGuangzhouGuangdong 510060PR China Tianjin Medical University Cancer Institute and Hospital National Clinical Research Center for CancerTianjin 300060PR China Key Laboratory of Cancer Immunology and Biotherapy Tianjin 300060PR China Department of Biotherapy/Immunology Tianjin Medical University Cancer Institute and HospitalTianjin 300060PR China Department of Medical Oncology Sun Yat-sen University Cancer CenterGuangzhou 510060PR China Department of Oncology the First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450052PR China Department of Tumor Biological Treatment the Third Affiliated Hospital of Soochow UniversityChangzhouJiangsu 213003PR China jiangsu Engineering Research Center for Tumor Immunotherapy ChangzhouJiangsu 213003PR China Institute for Cell Therapy of Soochow University ChangzhouJiangsu 213003PR China Department of Stem Cell&Regenerative Medicine State Key Laboratory of TraumaBurn and Combined InjuryDaping HospitalArmy Medical UniversityChongqing 400042PR China Imaging Diagnosis and Interventional Center Sun Yat-sen University Cancer CenterGuangzhouGuangdong 510060PR China

Fluoropyrimidine-based combination chemotherapy plus targeted therapy is the standard initial treatment for unresectable metastatic colorectal cancer(mCRC),but the prognosis remains *** phase 3 trial(***:NCT03950154)assessed the efficacy and adverse events(AEs)of the combination of PD-1 blockade-activated DC-CIK(PD1-T)cells with XELOX plus bevacizumab as a first-line therapy in patients with mCRC.A total of 202 participants were enrolled and randomly assigned in a 1:1 ratio to receive either first-line XELOX plus bevacizumab(the control group,n=102)or the same regimen plus autologous PD1-T cell immunotherapy(the immunotherapy group,n=100)every 21 days for up to 6 cycles,followed by maintenance treatment with capecitabine and *** main endpoint of the trial was progression-free survival(PFS).The median follow-up was 19.5 *** PFS was 14.8 months(95%CI,11.6-18.0)for the immunotherapy group compared with 9.9 months(8.0-11.8)for the control group(hazard ratio[HR],0.60[95%CI,0.40-0.88];p=0.009).Median overall survival(OS)was not reached for the immunotherapy group and 25.6 months(95%CI,18.3-32.8)for the control group(HR,0.57[95%CI,0.33-0.98];p=0.043).Grade 3 or higher AEs occurred in 20.0%of patients in the immunotherapy group and 23.5%in the control groups,with no toxicity-associated deaths *** addition of PD1-T cells to first-line XELOX plus bevacizumab demonstrates significant clinical improvement of PFS and OS with well tolerability in patients with previously untreated mCRC.

关键词： PD1 immunotherapy bevacizumab

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Simultaneous 50cm/10cm Single-pulse Polarization Observations of PSR j0953+0755

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Research in Astronomy and Astrophysics 2022年第6期22卷 52-63页

作者： Rukiye Rejep Wen-Ming Yan na Wang Xinjiang Astronomical Observatory Chinese Academy of SciencesUrumqi 830011China University of Chinese Academy of Sciences Beijing 100049China Key Laboratory of Radio Astronomy Chinese Academy of SciencesNanjing 210008China Xinjiang Key Laboratory of Radio Astrophysics Urumqi 830011China

We report on simultaneous single-pulse observations of PSR j0953+0755 at 732 and 3100 MHz made using the Parkes 64-m radio telescope at two *** non-simultaneous 1369 MHz observation has also been analyzed to compare polarization properties of this pulsar at different *** previously reported lowlevel bridge emission between the interpulse and the main pulse is notably present at 732 ***,the bridge emission becomes very weak or undetectable at higher *** cross-correlation analysis of simultaneous observations indicates that the total intensity of single pulses is highly correlated,which implies the same emission mechanism is responsible for the two *** confirm that the abrupt position angle jumps are non-orthogonal in this pulsar which probably result from the overlapping emission from two non-orthogonal polarization modes and the separation between different polarization modes is *** all three frequencies,the dominant modes are clearly associated with negative values of circular polarization,but the association seems unclear for weak modes.

关键词： stars neutron-(stars)pulsars general-(stars)pulsars individual(PSR j0953+0755)

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Development and application of phase diagrams for Li-ion batteries using CALPHAD approach

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Progress in natural Science:Materials International 2019年第3期29卷 265-276页

作者： na Li Dajian Li Weibin Zhang Keke Chang Feng Dang Yong Du Hans j.Seifert Key Laboratory for Liquid-Solid Structural Evolution and Processing of Materials (Ministry of Education) Shandong University Institute for Applied Materials–Applied Materials Physics (IAM-AWP) Karlsruhe Institute of Technology (KIT) Engineering Laboratory of Advanced Energy Materials Ningbo Institute of Materials Technology and Engineering Chinese Academy of Sciences State Key Laboratory of Powder Metallurgy Central South University

Phase diagrams provide fundamental knowledge about design map of new electrode materials for Li-ion batteries. The CALPHAD (CALculation of PHAse Diagrams) approach is widely applied to the development of phase diagrams and property diagrams in a thermodynamic language. Within the CALPHAD framework, the theoretical modeling can be performed to predict phase equilibria, thermodynamics, electrochemical and physical properties of electrodes. This review provides the successful application of high quality calculated phase diagrams and thermodynamic property diagrams in CALPHAD investigation to both cathodes and anodes of Li-ion batteries, including Li–Co–O, Li–Ni–O, Li–Co–Ni–O, Li–Mn–O, Li–Cu–O, Li–Si, Li–Sb and Li–Sn systems with. The intensive CALPHAD-type research may also predict electrochemical properties, cell performance of the Li-ion batteries to achieve more efficient development of electrode materials.

关键词： Li-Ion battery Phase diagram CALPHAD Thermodynamic property Electrochemical behavior

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变频调速器逆变单元故障自动化诊断仿真

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计算机仿真 2024年第3期41卷 76-80页

作者：董燕丽刘攀白娜晋中信息学院智能工程学院山西晋中030800 桂林电子科技大学海洋工程学院广西北海536000

变频器在切换过程中会产生过电流冲击,引起电网电压波动,造成设备逆变单元频繁跳闸。为降低变频调速器的故障风险,提出变频调速器逆变单元故障自动化诊断方法。通过比较逆变单元正常状态下电压信号与故障发生后电压信号,建立谱残差预测... 详细信息

变频器在切换过程中会产生过电流冲击,引起电网电压波动,造成设备逆变单元频繁跳闸。为降低变频调速器的故障风险,提出变频调速器逆变单元故障自动化诊断方法。通过比较逆变单元正常状态下电压信号与故障发生后电压信号,建立谱残差预测方程。利用最小二乘估计法,更新逆变单元信号谱残差预测值,通过加窗短时傅里叶变换以及哈特莱算法,提取出逆变器故障特征,将故障特征输入BP神经网络中训练,输出值即为故障诊断结果。实验结果表明,迭代次数达到200次研究方法的函数损失率降至0,且故障诊断精度接近100%。

关键词：变频调速器逆变单元故障诊断特征提取神经网络

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化湿败毒散通过减少胶原交联改善肺纤维化的机制研究

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中华中医药杂志 2024年第5期39卷 2335-2342页

作者：李俐霜马语蔓任星周娜王毅孙娅楠中国中医科学院医学实验中心北京100700

目的:探讨中药化湿败毒散对小鼠肺纤维化的治疗作用及其潜在机制。方法:50只C57BL/6j小鼠随机分成正常组、假手术组、博来霉素组、化湿败毒散组(早干预)、化湿败毒散组(晚干预)5组。用博来霉素气管注射,建立小鼠特发性肺纤维化(IPF)模... 详细信息

目的:探讨中药化湿败毒散对小鼠肺纤维化的治疗作用及其潜在机制。方法:50只C57BL/6j小鼠随机分成正常组、假手术组、博来霉素组、化湿败毒散组(早干预)、化湿败毒散组(晚干预)5组。用博来霉素气管注射,建立小鼠特发性肺纤维化(IPF)模型。使用Rna-Seq筛选各组基因水平的异同,预测其涉及生物学过程和通路;记录小鼠的体质量变化;检测肺功能(呼吸频率和流量);拍摄肺部CT图像;用双光子荧光显微镜观测肺组织胶原形态;HE染色观察组织病理变化;qPCR检测肺组织中缺氧诱导因子1α(HIF1α)、赖氨酰氧化酶(LOX)、赖氨酰氧化酶样蛋白(LOXL)1~LOXL4以及赖氨酸羟化酶2(PLOD2)的mRna水平;免疫组化染色评估HIF1α、LOXL2、LOXL3、PLOD2在肺组织中的蛋白表达水平。结果:转录组结果表明化湿败毒散主要影响了胶原蛋白合成、细胞外基质相互作用和炎症反应,其机制可能涉及HIF-1通路;化湿败毒散组(早干预)相比于化湿败毒散组(晚干预)可加速体质量恢复;改善肺部胶原交联,减缓纤维化进程;对呼吸频率和流量恢复具有正向调节作用;同时可降低小鼠肺组织中HIF1α、LOX、LOXL3的基因表达水平(P<0.05,P<0.01);下调HIF1α、LOXL2、LOXL3、PLOD2的蛋白表达水平(P<0.01)。结论:化湿败毒散可降低肺纤维化中的胶原交联水平,减缓肺纤维化进程。

关键词：肺纤维化化湿败毒散胶原交联机制研究缺氧诱导因子1通路

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计算解析异常代谢对乳腺癌微环境重塑的调控机制

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遗传 2024年第10期46卷 871-885页

作者：万羽鑫朱欣雨赵宇孙娜江天彤妃徐娟哈尔滨医科大学生物信息科学与技术学院哈尔滨150081

肿瘤微环境(tumor microenvironment,TME)中T细胞亚群的组成和肿瘤特异的T细胞互作促进了乳腺癌异质性的形成。此外,肿瘤的异常代谢通常与T细胞的抗肿瘤免疫功能失调关联密切,识别影响免疫细胞互作的关键代谢基因能够为乳腺癌治疗提供... 详细信息

肿瘤微环境(tumor microenvironment,TME)中T细胞亚群的组成和肿瘤特异的T细胞互作促进了乳腺癌异质性的形成。此外,肿瘤的异常代谢通常与T细胞的抗肿瘤免疫功能失调关联密切,识别影响免疫细胞互作的关键代谢基因能够为乳腺癌治疗提供潜在靶点。本研究利用乳腺癌单细胞转录组数据,重点研究了乳腺癌发展过程中肿瘤特异性T细胞亚群及其互作子网,进一步评估肿瘤特异性激活的T细胞亚群的代谢通路活性。结果显示,肠促胰岛素的合成、分泌和失活的代谢通路以及果糖分解代谢通路显著影响了多个T细胞亚群的互作。整合肿瘤中T细胞显著上调以及影响互作的代谢通路,依此筛选出核心T细胞互作相关的异常代谢基因,并进一步构建乳腺癌风险评估模型。利用异常代谢显著相关预后基因表达谱与药物IC50值预测靶向药物,最终获得潜在靶向药物GSK-j4、PX-12等。本研究整合分析乳腺癌微环境中T细胞互作的重塑与代谢通路异常在癌症恶性进展中的作用,为新型乳腺癌抗癌疗法提供线索。

关键词：乳腺癌微环境重塑异常代谢 T细胞互作

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Peroxisome proliferator-activated receptor-γ is essential in the pathogenesis of gastric carcinoma

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World journal of Gastroenterology 2009年第31期15卷 3874-3883页

作者： Xiu-Mei Ma Hong Yu na Huai Department of Pathology The First Affiliated Hospital of Inner Mongolia Medical College Huhhot 010059 Inner Mongolia Autonomous Region China Department of Pathology School of Basic Medical Sciences of Inner Mongolia Medical College Huhhot 010059 Inner Mongolia Autonomous Region China The Graduate Faculty of Inner Mongolia Medical College Huhhot 010059 Inner Mongolia Autonomous Region China

AIM： To investigate whether peroxisome proliferatoractivated receptor γ （PPAR-γ） is expressed in human gastric carcinoma and whether PPAR-γ, is a potential target for gastric carcinoma therapy. METHODS： PPAR-γ protein in gastric carcinoma was examined by immunohistochemistry. In the gastric carcinoma cell line MGCS03, PPAR-7, survivin, Skp2 and p27 protein and mRna were examined by Western blotting and real-time reverse transcription-polymerase chain reaction, respectively; proliferation was examined by MTT; apoptosis was examined by chromatin staining with Hoechst 33342 and fluorescence activated cell sorting （FACS）. and cell cycle was examined by FACS; the knockdown of PPAR-γ was done by Rna interference. RESULTS： A high level of expression of PPAR-γ was observed in human gastric carcinoma and in a human gastric carcinoma cell line MGCS03. The PPAR-γ agonist 15-deoxy-△12,14-prostaglandin j2 （15d-PGj2）inhibited growth, and induced apoptosis and G1/G0 cell cycle arrest in MGC803 cells in a concentration-dependent and time-dependent manner. The effect of 15d-PGj2 on MGC803 cells was not reversed by the selective and irreversible antagonist GW9662 for PPAR-γ. Furthermore, survivin and Skp2 expression were decreased, whereas p27 expression was enhanced following 15d-PGj2 treatment in a dose-dependent manner in MGC803 cells. Interestingly, we also found that small interfering Rna for PPAR-γ inhibited growth and induced apoptosis in MGC803 cells. The inhibition of PPAR-γ function may be a potentially important and novel modality for treatment and prevention of gastric carcinoma. CONCLUSION： A PPAR-γ agonist inhibited growth of human gastric carcinoma MGC803 cells by inducing apoptosis and G1/G0 cell cycle arrest with the involvement of survivin, Skp2 and p27 and not via PPAR-γ.

关键词： Peroxisome proliferator-activated receptor Gastric cancer Apoptosis Cell cycle

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Bismuth-based halide double perovskite Cs_(2)KBiCl_(6): Disorder and luminescence

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Chinese Chemical Letters 2024年第5期35卷 504-508页

作者： Pan Liu Yanming Sun Alberto j.Fernández-Carrión Bowen Zhang Hui Fu Lunhua He Xing Ming Congling Yin Xiaojun Kuang MOE Key Laboratory of New Processing Technology for Nonferrous Metal and Materials Guangxi Key Laboratory of Optic and Electronic Materials and DevicesCollege of Materials Science and EngineeringGuilin University of TechnologyGuilin 541004China Analytical Instrumentation Center College of Chemistry and Molecular EngineeringPeking UniversityBeijing 100871China Spallation Neutron Source Science Center Dongguan 523803China Chinese Academic of Science Institute of PhysicsBeijing National Laboratory for Condensed Matter PhysicsBeijing 100190China Songshan Lake Materials Laboratory Dongguan 523808China College of Science Guilin University of TechnologyGuilin 541004China College of Chemistry and Bioengineering Guilin University of TechnologyGuilin 541004China

A new bismuth-based halide double perovskite Cs_(2)KBiCl_(6) was isolated successfully through solid-state reactions and investigated using X-ray and neutron *** than an ordered structure,the crystal structure consists of shifted Cs,K,Bi,and Cl sites from the ideal positions with fractional occupancy in compensation,leading to variable local coordination of Cs^(+)ions,as revealed by^(133)Cs solid-state nuclear magnetic resonance ***_(2)KBiCl_(6) displays volume hysteresis at 5-298 K range upon heating and *** Cs_(2)KBiCl_(6) has a direct bandgap of 3.35(2)eV and red-shift luminescence of around 600 nm upon Mn doping compared with the na *** stabilization of disordered structure in Cs_(2)KBiCl_(6) is related to two factors including the large-sized K^(+)cation which prefers to coordinate with more than six Cl^(-),and the Bi^(3+)with 6s^(2) lone pair which has a preference for a local asymmetric *** findings could have general application and help to understand the structure and property of halide perovskites.

关键词： Halide perovskite Double perovskite Luminescence Disordered structure Direct bandgap

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