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湖南省稻瘟病菌无毒基因鉴定

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南方农业学报 2016年第9期47卷 1500-1505页

作者：刘翔任佐华陈娟芳毛锐戴良英刘二明湖南农业大学植物保护学院/植物病虫害生物学与防控湖南省重点实室/南方粮油作物协同创新中心长沙410128

【目的】了解湖南省稻瘟病菌无毒基因组成,为培育水稻新品种及合理布局抗稻瘟病品种提供参考依据。【方法】采用2014年分离自湖南桃江病圃丽江新团黑谷（LTH）上的120个稻瘟病菌单孢菌株室内离体针刺接种于24个已知抗瘟基因的水稻近等... 详细信息

【目的】了解湖南省稻瘟病菌无毒基因组成,为培育水稻新品种及合理布局抗稻瘟病品种提供参考依据。【方法】采用2014年分离自湖南桃江病圃丽江新团黑谷（LTH）上的120个稻瘟病菌单孢菌株室内离体针刺接种于24个已知抗瘟基因的水稻近等基因系（nils）上,对供试菌株进行无毒基因鉴定。【结果】120个稻瘟病菌含有全部24个无毒基因,在24个无毒基因中,出现频率高于40.00%的有Avr-Pita和Avr-Pish;出现频率在30.00%-40.00%的无毒基因有Avr-Piz、Avr-Piz5、Avr-Pi3和Avr-Pi9;其余无毒基因出现频率均较低,特别是Avr-Piks、Avr-Pikp、Avr-Pib、Avr-Pi12和Avr-Pi11出现频率均低于10.00%。【结论】含抗性基因Pi-ta和Pi-sh的水稻品种可在湖南省推广利用;含抗性基因Pi-z、Pi-z5、Pi-3和Pi-9的水稻品种在湖南推广时应慎重考虑;含抗性基因Pi-ks、Pi-kp、Pi-b、Pi-12和Pi-11的水稻品种不宜在湖南省推广利用。

关键词：稻瘟病菌近等基因系离体接种无毒基因湖南省

Accretion in strong field gravity with eXTP

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Science China(Physics,Mechanics & Astronomy) 2019年第2期62卷 45-73页

作者： Alessandra De Rosa Phil Uttley LiJun Gou Yuan Liu Cosimo Bambi Didier Barret Tomaso Belloni Emanuele Berti Stefano Bianchi Ilaria Caiazzo Piergiorgio Casella Marco Feroci Valeria Ferrari Leonardo Gualtieri Jeremy Heyl Adam Ingram Vladimir Karas FangJun Lu Bin Luo Giorgio Matt Sara Motta Joseph Neilsen Paolo Pani Andrea Santangelo XinWen Shu JunFeng Wang Jian-Min Wang YongQuan Xue YuPeng Xu WeiMin Yuan YeFei Yuan Shuang-Nan Zhang Shu Zhang Ivan Agudo Lorenzo Amati nils Andersson Cristina Baglio Pavel Bakala Altan Baykal Sudip Bhattacharyya Ignazio Bombaci NiccolóBucciantini Fiamma Capitanio Riccardo Ciolfi Wei K.Cui Filippo D'Ammando Thomas Dauser Melania Del Santo Barbara De Marco Tiziana Di Salvo chris Done Michal Dovciak Andrew C.Fabian Maurizio Falanga Angelo Francesco Gambino Bruce Gendre Victoria Grinberg Alexander Heger Jeroen Homan Rosario Iaria JiaChen Jiang ChiChuan Jin Elmar Koerding Manu Linares Zhu Liu Thomas J.Maccarone Julien Malzac Antonios Manousakis Frédéric Marin Andea Marinucci Missagh Mehdipour Mariano Méndez Simone Migliari Cole Miller Giovanni Miniutti Emanuele Nardini Paul T.O'Brien Julian P.Osborne Pierre Olivier Petrucci Andrea Possenti Alessandro Riggio Jerome Rodriguez Andrea Sanna LiJing Shao Malgosia Sobolewska Eva Sramkova Abigail L.Stevens Holger Stiele Giulia Stratta Zdenek Stuchlik Jiri Svoboda Fabrizio Tamburini Thomas M.Tauris Francesco Tombesi Gabriel Torok Martin Urbanec Frederic Vincent QingWen Wu Feng Yuan Jean J.M.in't Zand Andrzej A.Zdziarski XinLin Zhou INAF-Istituto di Astrofisica e Planetologie Spaziali Via Fosso del Cavaliere Anton Pannekoek Institute for Astronomy University of Amsterdam National Astronomical Observatories Chinese Academy of Science Institute of High Energy Physics Chinese Academy of Science Center for Field Theory and Particle Physics and Department of Physics Fudan University IRAP Universitéde ToulouseCNRSUPSCNES INAF-Osservatorio Astronomico di Brera Department of Physics and Astronomy The University of MississippiUniversity Dipartimento di Matematica e Fisica Universitàdegli studi Roma Tre University of British Columbia INAF-Osservatorio Astronomico di Roma INFN-Roma 2 c/o Dipartimento di Fisica II Universit?di Roma"Tor Vergata" Dipartimento di Fisica Universitàdegli Studi di Roma"La Sapienza"&Sezione INFN Roma1 Department of Physics University of OxfordClarendon Laboratory Astronomical Institute Academy of Sciences School of Astronomy and Space Science Nanjing University MIT Kavli Institute for Astrophysics and Space Research Institut für Astronomie und Astrophysik Eberhard Karls Universität Department of Physics Anhui Normal University Department of Astronomy and Institute of Theoretical Physics and Astrophysics Xiamen University CAS Key Laboratory for Research in Galaxies and Cosmology Department of AstronomyUniversity of Science and Technology of China Instituto de Astrofísica de Andalucia(CSIC) Glorieta de la Astronomía s/n INAF-Istituto di Astrofisica Spaziale e Fisica cosmica di Bologna Mathematical Sciences and STAG Research Centre University of Southampton New York University Abu Dhabi Institute of Physics and Research Centre for Computational Physics and Data Processing Faculty of Philosophy&ScienceSilesian University in OpavaíBezru?ovo nám. Department of Physics Middle East Technical University Department of Astronomy and Astrophysics Tata Institute of Fundamental Research Dipartimento di Fisica Universit?di Pisa&INFN INAF-Osservatorio Astrofisico di Arcetri INAF-Osservatorio Astronomico di Padova INFN-TIFPA Trento Ins

In this paper we describe the potential of the enhanced X-ray Timing and Polarimetry(eXTP) mission for studies related to accretion flows in the strong field gravity regime around both stellar-mass and supermassive black-holes. eXTP has the unique capability of using advanced "spectral-timing-polarimetry" techniques to analyze the rapid variations with three orthogonal diagnostics of the flow and its geometry, yielding unprecedented insight into the inner accreting regions, the effects of strong field gravity on the material within them and the powerful outflows which are driven by the accretion process.

关键词： X-ray black holes physics accretion

湖南桃江稻瘟病菌遗传多样性分析及无毒基因的鉴定

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西南农业学报 2019年第9期32卷 2040-2045页

作者：贺雄丁朝辉周瑚朱华珺李俊俊丁宇倩胡立冬任佐华刘二明湖南农业大学植物保护学院湖南长沙410128 植物病虫害生物学与防控湖南省重点实验室湖南长沙410128 南方粮油作物协同创新中心湖南长沙410128 湖南省桃江县农业局植保植检站湖南桃江413400

【目的】为明确湖南桃江地区稻瘟病菌遗传多样性和无毒基因的组成及变化趋势。【方法】利用8对SSR引物对2017年从湖南桃江病圃多个晚稻品种上分离纯化的29个稻瘟病单孢菌株进行遗传多样性分析,并利用18个已知抗瘟基因的水稻近等基因系(N... 详细信息

【目的】为明确湖南桃江地区稻瘟病菌遗传多样性和无毒基因的组成及变化趋势。【方法】利用8对SSR引物对2017年从湖南桃江病圃多个晚稻品种上分离纯化的29个稻瘟病单孢菌株进行遗传多样性分析,并利用18个已知抗瘟基因的水稻近等基因系(nils)对其中的19个特异性稻瘟病菌株进行无毒基因鉴定。【结果】在相似系数0.70,差异系数0.30水平下,29个供试菌株可划分为7个宗谱,优势宗谱Ⅰ有12个菌株,占参测菌数的41.3%,宗谱Ⅴ有7个菌株,占总菌数的24.1%,其余5个宗谱分别有1?3个菌株,占总菌数的34.6%;通过无毒基因鉴定18个菌株致病频率(致病频率=感病水稻数/所有测试水稻品种数XlOO%)在35%?75%。强致病力菌株(致病频率≥70%)3株,占总菌数的15.7%;较强致病力菌株(70%)致病频率≥50%)7株,占总菌数36.8%;中等致病力菌株(50%>致病频率3=20%)9株,占总菌数的47.5%;供试菌株平均致病频率为49.86%。19个菌株对nils水稻品系毒力频率(毒力频率=对测试水稻品系有毒力的菌株数/所有菌株数XlOO%)超过70%的抗性基因为Pi-k^s、Pi-z^t、Pi-ta,属强毒力菌株;毒力频率介于50%?70%的抗性基因为Pi-k^p、Pi-z^5、Pi-t、Pi-sh、Pi-5,,属于中等毒力菌株;对其余抗性基因的毒力频率低于50%。【结论】湖南桃江稻瘟病菌群体具有丰富的遗传多样性,而且存在复杂的致病性分化;抗性基因Pi-3、Pi-i、Pi-11、Pi-19、Pi-12对桃江地区稻瘟病菌抗性优良,可在抗病育种中加以利用。

关键词：稻瘟病菌 SSR分子标记遗传多样性无毒基因