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Structural insights of phosphorylated into the recognition FUNDC1 by LC3B in mitophagy

Protein & Cell 2017年第1期8卷 25-38页

作者： Mengqi Lv chongyuan wang Fudong Li Junhui Peng Bin Wen Qingguo Gong Yunyu Shi Yajun-Tang Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences University of Science and Technology of China Hefei 230027 China

Mitophagy is an essential intracellular process that eliminates dysfunctional mitochondria and maintains cellular homeostasis. Mitophagy is regulated by the post-translational modification of mitophagy receptors. Fun14 domain-containing protein 1 （FUNDC1） was reported to be a new receptor for hypoxia-induced mitophagy in mammalian cells and interact with micro-tubule-associated protein light chain 3 beta （LC3B） through its LC3 interaction region （LIR）. Moreover, the phosphorylation modification of FUNDC1 affects its binding affinity for LC3B and regulates selective mitophagy. However, the structural basis of this regulation mechanism remains unclear. Here, we present the crystal structure of LC3B in complex with a FUNDCI LIR peptide phosphorylated at Ser17 （pS17）, demonstrating the key residues of LC3B for the specific recognition of the phosphorylated or dephosphorylated FUNDC1. Intriguingly, the side chain of LC3B Lys49 shifts remarkably and forms a hydrogen bond and electrostatic interaction with the phosphate group of FUNDC1 pS17. Alternatively, phosphorylated Tyr18 （PY18） and Ser13 （PS13） in FUNDC1 significantly obstruct their interaction with the hydrophobic pocket and Arg10 of LC3B, respectively. Structural observations are further validated by mutation and isothermal titration calorimetry （ITC） assays. Therefore, our structural and biochemical results reveal a working model for thespecific recognition of FUNDCI by LC3B and imply that the reversible phosphorylation modification of mitophagy receptors may be a switch for selective mitophagy.

关键词： microtubule-associated protein light chain 3 beta (LC3B) fun14 domain-containing protein 1 (FUNDC1) phosphorylation selective mitophagy

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Impact of Environmental Regulation on Industrial Upgrading: Analysis Based on Panel Data of 30 Provinces in China

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Meteorological and Environmental Research 2020年第3期11卷 21-31,37页

作者： Jianqin LI chongyuan wang School of Economics Zhejiang UniversityHangzhou 310058China

Environmental regulation and industrial upgrading are the key to achieve win-win results for both economy and the environment. After environmental regulation tools are divided into market incentive and command control types,based on the provincial-level data of 30 provinces( cities and regions) in China from 2004 to 2016,the impact of environmental regulation on industrial upgrading and its transmission paths are empirically tested through an intermediary effect model. Technological innovation,FDI and capital market development all meet intermediary conditions,and the market incentive type is more dependent on technological innovation,while the command control type is more dependent on FDI and capital market *** impact of the two environmental regulation tools on industrial upgrading is further studied. The results show that there is an " inverted U-shaped" relationship between the command control type and industrial upgrading,while there is a " U-shaped" relationship between the market incentive type and industrial upgrading,and there are also certain regional differences in the impact of environmental regulation on industrial upgrading.

关键词： Command control environmental regulation Market incentive environmental regulation Industrial upgrading Conduction paths Regional differences

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清代徽商合墨及盘、帐单——以《徽州文书》第一辑为中心

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中国社会经济史研究 2006年第4期 28-38页

作者：汪崇筼桂林市安新小区广西桂林541002

依据合墨及相关史料,可勾勒出徽商创业的曲折经历,以及经营的特点。由同和、兆成盘单可知,这是两个在购销环节上,均采用预付款制的商业字号;在多数情况下,其资本年利率在10——20%之间。亦盛升字号则采用赊销制。该字号于同治十年年终... 详细信息

依据合墨及相关史料,可勾勒出徽商创业的曲折经历,以及经营的特点。由同和、兆成盘单可知,这是两个在购销环节上,均采用预付款制的商业字号;在多数情况下,其资本年利率在10——20%之间。亦盛升字号则采用赊销制。该字号于同治十年年终被客户拖欠的银两,已超出字号所具备的正本总额。但该年度字号的资本利率仍达20%。帐单是徽州商业文书中一个难以阅读的部分。通过对三份不同帐单的探讨(其中一份无名帐单可能是草稿),相信对阅读徽州商业文书有一定帮助。

关键词：清代徽商合墨盘单帐单

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Expression of PADI4 in hepatocellular carcinoma

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The Chinese-German Journal of Clinical Oncology 2009年第8期8卷 453-455页

作者： Yang Lv Yan Xia Yaohua wang chongyuan Cai Division I of General Surgery Jingmen Second People＇s Hospital Jinmen 448000 China

Objective： The aim of the research was to study peptidylarginine deiminase type 4 （PAD4/PADI4） expression and its tumodgenic mechanism in hepatocellular carcinomas. Methods： Expressions of PADI4 and p53 were investigated in tumors and non-tumor tissues by Western blot in patients with hepatocellular carcinomas. We constructed plasmid of PADI4-Flag and transfected it in Hela cells to investigate the mechanism. Results： Western blot analysis showed higher PADI4 expression in hepatocellular carcinomas than in the surrounding healthy tissues. Furthermore, by Western blot, we detected decreased p53 levels in the tumor tissues of patients with hepatocellular carcinomas compared to surrounding healthy tissues. In Hela cells transfected with PcDNA3.0-Flag-PADI4 plasmid, the expression of p53 decreased obviously. Conclusion： Our results suggest that PADI4 elevated in the tissues of hepatocellular carcinomas and induced tumorigenic by down-regulating p53 expression.

关键词： hepatocellular carcinoma peptidylarginine deiminase type 4 (PAD4/PADI4) tumorigenic mechanism

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进水流量对新型储热水箱分层特性影响的实验研究

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化工进展 2016年第2期35卷 403-408页

作者：王崇愿王子龙张华车敏上海理工大学制冷及低温工程研究所上海200093 海尔集团技术研发中心山东青岛266103

储热水箱被广泛使用在太阳能集热系统以及家用电加热热水器中,是决定集热系统和热水器性能的关键因素之一,储热水箱分层效果越好,越能提高集热系统效率及热水器的热水出水量。本文设计了一种新型均流器,安装在圆柱形储热水箱底部,并搭... 详细信息

储热水箱被广泛使用在太阳能集热系统以及家用电加热热水器中,是决定集热系统和热水器性能的关键因素之一,储热水箱分层效果越好,越能提高集热系统效率及热水器的热水出水量。本文设计了一种新型均流器,安装在圆柱形储热水箱底部,并搭建了一套储热水箱分层特性测试实验台。在初始水温50℃、进水温度20℃的工况下,分析对比了3组不同流量(0.69L/min、2.14L/min、6.17L/min)时储热水箱的分层特性,结果显示,大流量比小流量温度曲线的斜率更大,温度下降速度更快。同时,基于热力学定律,分别计算了3组流量水箱的取出效率、用能效率、出于能量品质的考虑而采用的?效率,在流量为0.69L/min、2.14L/min、6.17L/min时的取出效率分别为91.8%、95.7%、94.3%,用能效率分别为96.6%、98.6%、97.5%、?效率分别为78.5%、83.1%、77.0%。

关键词：储热水箱分层效率流动热力学

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突面脑膜瘤合并硬脑膜“尾”征的手术意义

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中华神经外科杂志 1997年第3期13卷 147-149页

作者：李龄王承缘同济医科大学附属同济医院神经外科

目的：研究突面脑膜瘤复发的病因。方法：１０例突面脑膜瘤经手术切除与病理证实。术前皆作ＭＲＩ的ＧＤ－ＤＴＰＡ增强检查，证实为脑膜瘤并有硬脑膜“尾”征，病理学检查有肿瘤细胞浸润，作包括硬脑膜“尾”的肿瘤手术Ｉ级切除。结果... 详细信息

目的：研究突面脑膜瘤复发的病因。方法：１０例突面脑膜瘤经手术切除与病理证实。术前皆作ＭＲＩ的ＧＤ－ＤＴＰＡ增强检查，证实为脑膜瘤并有硬脑膜“尾”征，病理学检查有肿瘤细胞浸润，作包括硬脑膜“尾”的肿瘤手术Ｉ级切除。结果：效果良好，随访６月至１年无复发。结论：证实脑膜瘤的细胞浸润至硬脑膜有助于神经外科医师确切地作包括“尾”在内的Ｉ级切除术。

关键词：脑膜瘤硬脑膜“尾”征外科手术

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Structural insights into the interaction of the ribosomal P stalk protein with a type II ribosome-inactivating protein Ricin

Structural insights into the interaction of the ribosomal P ...

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中国生物化学与分子生物学会2016年全国学术会议

作者： Xiaojiao Fan Yuwei Zhu chongyuan wang Liwen Niu Maikun Teng Xu Li Hefei National Laboratory for Physical Sciences at Microscale Innovation Center for Cell Signaling Network School of Life ScienceUniversity of Science and Technology of China Key Laboratory of Structural Biology Hefei Science Center of CAS Chinese Academy of cience

Ricin is a type II ribosome-inactivating protein(RIP).It depurinates the A4324 at the sarcin-ricin loop of 28S r RNA,which thereby inactivates ribosome by preventing elongation factors binding to the GTPase activation *** studies have disclosed that the conserved C-terminal domain(CTD)of eukaryotic ribosomal P stalk proteins is involved in the targeting

关键词： ribosome-inactivation protein Ricin ribosomal P stalk protein sarcin-ricin loop

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A novel RNA-binding mode of the YTH domain reveals the mechanism for recognition of determinant of selective removal by Mmi1

A novel RNA-binding mode of the YTH domain reveals the mecha...

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第五届中国结构生物学学术讨论会

作者： chongyuan wang Yuwei Zhu Hongyu Bao Yiyang Jiang Chao Xu Jihui Wu Yunyu Shi Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences University of Science and Technology of China

The YTH domain-containing protein Mmi1, together with other factors, constitutes the machinery used to selectively remove meiosis-specific m RNA during the vegetative growth of fission yeast. Mmi1 directs meiotic m RNAs to the nuclear exosome for degradation by recognizing their DSR（determinant of selective removal） motif. Here, we present the crystal structure of the Mmi1 YTH domain in the apo state and in complex with a DSR motif, demonstrating that the Mmi1 YTH domain selectively recognizes the DSR motif. Intriguingly, Mmi1 also contains a potential m6A（N6-methyladenine）-binding pocket, but its binding of the DSR motif is dependent on a long groove opposite the m6A pocket. The DSR-binding mode is distinct from the m6A RNA-binding mode utilized by other YTH domains. Furthermore, the m6A pocket cannot bind m6A RNA. Our structural and biochemical experiments uncover the mechanism of the YTH domain in binding the DSR motif and help to elucidate the function of Mmi1.

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The complex structure of ARAP3 RhoGAP-RhoA reveals the molecular mechanism for specifically recognition of RhoA by ARAP3

The complex structure of ARAP3 RhoGAP-RhoA reveals the molec...

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第五届中国结构生物学学术讨论会

作者： Hongyu Bao Fudong Li chongyuan wang Na wang Yiyang Jiang Yajun Tang Jihui Wu Yunyu Shi Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences University of Science and Technology of China

ARAP3 is unique for its dual specific GAPs（GTPase activating protein） activity for Arf6 and RhoA, regulated by Ptd Ins（3,4,5）P3 and Rap1-GTP and involving in regulation of cell shape and adhesion. However, the clear interface between the Rho GAP domain and RhoA is unknown. Meanwhile, the substrate selectivity of the Rho GAP domain is also unclear. In this study, we solved the crystal structure of RhoA in complex with the Rho GAP domain of ARAP3. The complex structure presented the clear interface between the Rho GAP domain and RhoA. By analyzing the complex structure in combination with in vitro RhoA activity assay and ITC experiments, we found the crucial residues affecting the Rho GAP activity and the substrates selectivity among RhoA, Rac1 and Cdc42. The title should be in bold Arial（14 points）, with the initial letter capitalized. Authors will start on a new line and should be in regular Times New Roman（10 points）. Family name should be placed after the first name. Underline the name of the presenting author. Affiliation of authors should start on a new line in italics with address and e-mail address. Main text should be within 400 words in regular Times New Roman（12 points） and single-spaced. Insert a single blank line space between paragraphs.

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热电偶时间常数测量的不确定度评定

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传感技术学报 2020年第5期33卷 666-669页

作者：刘波王崇愿何永兴上海市计量测试技术研究院上海201203

热电偶因响应迅速而得到广泛应用,时间常数是表征其动态响应性能的关键参数。研制了水流环境温度传感器动态响应测量装置,首次评定了时间常数测量的不确定度。通过提出微小时间域内,电压示值与时间的线性关系,实现了温度参量不确定度向... 详细信息

热电偶因响应迅速而得到广泛应用,时间常数是表征其动态响应性能的关键参数。研制了水流环境温度传感器动态响应测量装置,首次评定了时间常数测量的不确定度。通过提出微小时间域内,电压示值与时间的线性关系,实现了温度参量不确定度向时间参量的传递。以工作用I级T型铠装热电偶为对象进行了不确定度评定,测量结果为τ=881 ms±32 ms(k=2)。评定结果表明:温度变送器的响应时间和示波器测量电压值的允差是不确定度的主要来源,研究结果可为实验测量提供指导。

关键词：计量学温度传感器动态响应时间常数不确定度评定

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