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柑橘指纹图谱研究进展

中国果树 2024年第7期 16-24页

作者：武晓晓吴仕蔓陈传武娄兵海唐艳冯静方海猛邓崇岭广西桂北特色经济作物种质创新与利用重点实验室广西柑橘育种与栽培技术创新中心广西特色作物研究院桂林541004 玉林师范学院广西大学农学院

对指纹图谱的定义、分子标记构建指纹图谱在其他果树上的研究现状、分子标记构建指纹图谱在柑橘上的研究现状、DNA分子标记检测方法进行了综述,重点介绍了RFLP、RAPD、AFLP、SSR、SNP等分子标记在构建柑橘指纹图谱方面的进展,指出存在... 详细信息

对指纹图谱的定义、分子标记构建指纹图谱在其他果树上的研究现状、分子标记构建指纹图谱在柑橘上的研究现状、DNA分子标记检测方法进行了综述,重点介绍了RFLP、RAPD、AFLP、SSR、SNP等分子标记在构建柑橘指纹图谱方面的进展,指出存在的问题并提出建议。

关键词：柑橘指纹图谱进展

NEW INSIGHTS INTO THE PHYLOGENY AND SPECIATION OF KUMQUAT (FORTUNELLA SPP.) BASED ON CHLOROPLAST SNP, NUCLEAR SSR AND WHOLE-GENOME SEQUENCING

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Frontiers of Agricultural Science and Engineering 2022年第4期9卷 627-641页

作者： Chenqiao ZHU Peng CHEN Junli YE Hang LI Yue HUANG Xiaoming YANG Chuanwu CHEN Chenglei ZHANG Yuantao XU Xiaoli WANG Xiang YAN Guangzhou deng Xiaolin JIANG Nan WANG Hongxing WANG Quan SUN Yun LIU Di FENG Min YU Xietian SONG Zongzhou XIE Yunliu ZENG Lijun CHAI Qiang XU chongling deng Yunjiang CHENG Xiuxin deng Key Laboratory of Horticultural Plant Biology(Ministry of Education) Huazhong Agricultural UniversityWuhan 430070China Fruit Industry Technology Guidance Stand of Ganzhou Ganzhou 341000China Guangxi Academy of Specialty Crops/Guangxi Citrus Breeding and Cultivation Research Center of Engineering Technology Guilin 541004China Ganzhou Citrus Research Institute Ganzhou 341000China

Kumquat(Fortunella spp.)is a fruit and ornamental crop worldwide due to the palatable taste and high ornamental value of its *** Fortunella is classified into the economically important true citrus fruit tree group together with Citrus and Poncirus,few studies have been focused on its evolutionary *** this study,analysis of five chloroplast loci and 47 nuclear microsatellites(nSSR)loci from 38 kumquat and 10 citrus accessions revealed the independent phylogeny of Fortunella among citrus taxa,and that Fortunella mainly comprises two populations:CUL,cultivated Fortunella spp.(***,*** and ***);and HK,wild Hong Kong kumquat(Fortunella hindsii).Genomic analysis based on whole-genome SNPs indicated that the allele frequency of both pupations deviated from the neutral selection model,suggesting directional selection was a force driving their *** exhibited lower genomic diversity and higher linkage strength than HK,suggesting artificial selection involved in its origin.A high level of genetic differentiation(Fst=0.364)was detected and obviously asynchronous demographic changes were observed between CUL and *** on these results,a new hypothesis for the speciation of Fortunella is proposed.

关键词： Citrus Fortunella kumquat phylogenetics

基于重测序的广西姑婆山野生柑橘资源遗传演化及分类地位分析

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园艺学报 2022年第2期49卷 407-415页

作者：武晓晓陈传武刘萍唐艳邓崇岭广西特色作物研究院广西柑橘育种与栽培工程技术研究中心广西桂林541004

对4个广西姑婆山野生柑橘、1个枳、1个金柑以及14个其他柑橘进行全基因组重测序,每个样本测序数据量约为16 Gb,平均测序深度为54×。通过基因组比对、SNP检测、PCA主成分分析、邻接法构建系统发育进化树,分析广西贺州姑婆山野生柑橘资... 详细信息

对4个广西姑婆山野生柑橘、1个枳、1个金柑以及14个其他柑橘进行全基因组重测序,每个样本测序数据量约为16 Gb,平均测序深度为54×。通过基因组比对、SNP检测、PCA主成分分析、邻接法构建系统发育进化树,分析广西贺州姑婆山野生柑橘资源遗传多样性、群体结构及分类地位。从20份样品中,共检测出6 782 158个高质量SNP位点。基于这些SNP位点,根据PCA主成分分析和系统进化树分析结果,可将20份样本分为3类:广西姑婆山野柑与莽山野柑聚为一类,金柑与其他柑橘聚为一类,枳聚为单独一类。该研究表明姑婆山野生柑橘资源与莽山野柑一样具有原始性和独特性,应该为独立的属或者柑橘属中独立的种。

关键词：柑橘基因组进化多样性

Genome of Wild Mandarin and Domestication History of Mandarin

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Molecular Plant 2018年第8期11卷 1024-1037页

作者： Lun Wang Fa He Yue Huang Jiaxian He Shuizhi Yang Jiwu Zeng chongling deng Xiaolin Jiang Yiwen Fang Shaohua Wen Rangwei Xu Huiwen Yu Xiaoming Yang Guangyan Zhong Chuanwu Chen Xiang Yan Changfu Zhou Hongyan Zhang Zongzhou Xie Robert M. Larkin Xiuxin deng Qiang Xu Key Laboratory of Horticultural Plant Biotogy (Ministry of Education) Huazhong Agricultural University Wuhan 430070 P.R. China Horticulture institute Hunan Academy of Agricultural Sciences Changsha 410125 P.R. China Fruit Tree Research Institute Guangdong Academy of Agricultural Sciences Guangzhou 510640 P.R. China Guangxi Key Laboratory of Citrus Biology Guangxi Academy of Specialty Crops Guilin 541000 P.R. China Institute of Citrus Science Research of Ganzheu City Ganzhou 341000 P.R. China

Mandarin （Citrus reticulata） is one of the most important citrus crops worldwide. Its domestication is believed to have occurred in South China, which has been one of the centers of mandarin cultivation for four millennia. We collected natural wild populations of mandarin around the Nanling region and cultivated landraces in the vicinity. We found that the citric acid level was dramatically reduced in cultivated mandarins. To understand genetic basis of mandarin domestication, we de novo assembled a draft genome of wild mandarin and analyzed a set of 104 citrus genomes. We found that the Mangshan mandarin is a primitive type and that two independent domestication events have occurred, resulting in two groups of cultivated mandarins （MD1 and MD2） in the North and South Nanling Mountains, respectively. Two bottlenecks and two expansions of effective population size were identified for the MD1 group of cultivated mandarins. However, in the MD2 group there was a long and continuous decrease in the population size. MD1 and MD2 mandarins showed different patterns of interspecific introgression from cultivated pummelo species. We identified a region of high divergence in an aconitate hydratase （ACO） gene involved in the regulation of citrate content, which was possibly under selection during the domestication of mandarin. This study provides concrete genetic evidence for the geographical origin of extant wild mandarin populations and sheds light on the domestication and evolutionary history of mandarin.

关键词： Citrus Citric acid Domestication Genome Wild mandarin

A comprehensive proteomic analysis of elaioplasts from citrus fruits reveals insights into elaioplast biogenesis and function

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Horticulture Research 2018年第1期5卷 879-889页

作者： Man Zhu Jiajia Lin Junli Ye Rui Wang Chao Yang Jinli Gong Yun Liu chongling deng Ping Liu Chuanwu Chen Yunjiang Cheng Xiuxin deng Yunliu Zeng Key Laboratory of Horticultural Plant Biology(Ministry of Education) College of Horticulture and Forestry ScienceHuazhong Agricultural UniversityWuhan430070China Institute of Citrus Science Huazhong Agricultural UniversityWuhan430070China Shanghai Applied Protein Technology Co.Ltd Shanghai200233China Guangxi Citrus Research Institute Guangxi541004China

Elaioplasts of citrus peel are colorless plastids which accumulate significant amounts of ***,other functions of elaioplasts have not been fully characterized to ***,a LC–MS/MS shotgun technology was applied to identify the proteins from elaioplasts that were highly purified from young fruit peel of kumquat.A total of 655 putative plastid proteins were identified from elaioplasts according to sequence homology in silico and manual *** on functional classification via Mapman,~50%of the identified proteins fall into six categories,including protein metabolism,transport,and lipid *** note,elaioplasts contained ATP synthase and ADP,ATP carrier proteins at high abundance,indicating important roles for ATP generation and transport in elaioplast ***,a comparison of proteins between citrus chromoplast and elaioplast proteomes suggest a high level of functional ***,some distinctive protein profiles were also observed in both types of plastids notably for isoprene biosynthesis in elaioplasts,and carotenoid metabolism in *** conclusion,this comprehensive proteomic study provides new insights into the major metabolic pathways and unique characteristics of elaioplasts and chromoplasts in citrus fruit.

关键词： citrus comprehensive metabolism

新能源汽车企业高质量发展的驱动路径研究——基于TOE框架的组态分析

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新疆财经大学学报 2024年第2期 50-60页

作者：邓家姝黄重龄安立发甘肃政法大学甘肃兰州730070

文章基于TOE框架构建新能源汽车企业高质量发展的理论模型,采用fsQCA方法对沪深A股21家新能源汽车企业上市公司2017-2021年面板数据进行组态分析。研究发现:新能源汽车企业高质量发展受到多个前因要素的共同影响,不存在实现高质量发展... 详细信息

文章基于TOE框架构建新能源汽车企业高质量发展的理论模型,采用fsQCA方法对沪深A股21家新能源汽车企业上市公司2017-2021年面板数据进行组态分析。研究发现:新能源汽车企业高质量发展受到多个前因要素的共同影响,不存在实现高质量发展的必要条件;存在两组类型、三条路径驱动新能源汽车企业高质量发展,表明不同的前因条件组合可以实现行业高质量发展;无论是通过哪条路径实现高质量发展,高程度行业竞争压力及大规模政府补助是必不可缺的驱动条件;在企业存在高程度竞争压力和大规模政府补助的条件下,创新产出水平和组织韧性存在一定的替代关系,并以“殊途同归”的方式共同推动新能源汽车企业高质量发展。多重条件间组态效应的存在展现了新能源汽车企业高质量发展的复杂性,企业管理者应重点关注技术、组织、环境等多方面要素之间的协同联动,政府应加大资金支持力度、强化政策引导,鼓励新能源汽车企业通过转型等方式实现高质量发展。

关键词：新能源汽车企业 TOE框架 fsQCA 高质量发展

Down regulated expression of S_(2)-RNase attenuates self-incompatibility in “Guiyou No.1”pummelo

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Horticulture Research 2021年第1期8卷 2585-2599页

作者： ianbing Hu Qiang Xu Chenchen Liu Binghao Liu chongling deng Chuanwu Chen Zhuangmin Wei Muhammad Husnain Ahmad Kang Peng Hao Wen Xiangling Chen Peng Chen Robert MLarkin Junli Ye Xiuxin deng Lijun Chai Key Lab oratory of Horticultural Plant Biology Ministry of EducationCollege of Horticulture and Forestry SciencesHuazhong Agricultural UniversityWuhan 430070People's Republic of China Guangxi Engineering Research Center of Citrus Breeding and Culture Guangxi Academy of Specialty CropsGuilin 541004People's Republic of China Guangxi Subtropical Crops Research Institute Nanning 530001People's Republic of China Horticulture Research Institute Guangxi Academy of Agriculture SciencesNanning Investigation&Experiment Station of South Subtropical Fruit TreesMinistry of AgricultureNanning 530007 GuangxiPeople's Republic of China Horticultural Institute Hunan Academy of Agricultural SciencesChangsha 410125People's Republic of China

Self-incompatibility(SI)substantially restricts the yield and quality of ***,breeding and analyzing selfcompatible germplasm is of great theoretical and practical signi ficance for ***,we focus on the mechanism of a self-compatibility mutation in‘Guiyou No.1'pummelo(Citrus maxima),which is a spontaneous mutant of‘Shatian’pummelo(Citrus maxima,self-incompatibility).The rate of fruit set and the growth of pollen tubes in the pistil con firmed that a spontaneous mutation in the pistil is responsible for the self-compatibility of‘Guiyou No.1'.Segregation ratios of the S genotype in progeny,expression analysis,and western blotting validated that the reduced levels of S_(2)-RNase mRNA contribute to the loss of SI in‘Guiyou No.1'.Furthermore,we report a phased assembly of the‘Guiyou No.1'pummelo genome and obtained two complete and well-annotated S *** with an analysis of SV variations,methylation levels,and gene expression,we identi fied a candidate gene(CgHB40),that may in fluence the regulation of the S/^RNase *** data provide evidence that a mutation that affects the pistilled to the loss of SI in‘Guiyou No.1'by in fluencing a poorly understood mechanism that affects transcriptional *** work signi ficantly advances our understanding of the genetic basis of the SI system in citrus and provides information on the regulation of S-RNase genes.

关键词： citrus No.1 compatibility

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晚熟金柑新品种‘桂金柑2号’的选育

果树学报 2017年第10期34卷 1357-1360页

作者：邓崇岭邓光宙邓秀新刘冰浩唐艳陈传武邓俭友广西柑橘生物学重点实验室.广西特色作物研究院广西桂林541004 华中农业大学武汉430070 阳朔县俭友家庭农场广西阳朔541901

‘桂金柑2号’是由‘阳朔金柑’实生系选育出的晚熟金柑新品种。果实椭圆形,橙红色,光滑,油胞平生,果顶圆钝;单果质量24.31~34.05 g,果实横径33.80~37.11 mm,果实纵径39.30~43.58 mm,果形指数1.11~1.20,可食率97.81%~99.04%,每100 m L... 详细信息

‘桂金柑2号’是由‘阳朔金柑’实生系选育出的晚熟金柑新品种。果实椭圆形,橙红色,光滑,油胞平生,果顶圆钝;单果质量24.31~34.05 g,果实横径33.80~37.11 mm,果实纵径39.30~43.58 mm,果形指数1.11~1.20,可食率97.81%~99.04%,每100 m L果汁中含:维生素C 31.97~54.39 mg,枸橼酸0.23~0.49 g,全糖12.77~15.19 g,可溶性固形物15.2%~19.9%。种子数2.1~5.5粒,多胚,子叶淡绿色;风味浓郁,有微淡刺鼻味,果实汁多、较化渣,品质优。果实生育期180~195 d,在桂林阳朔12月中下旬至翌年1月中旬成熟。适合广西省桂林、柳州等地以及生态条件相似地区栽培,定植后第3年开花结果,丰产性好。

关键词：金柑新品种 '桂金柑2号’ 实生变异

铝掺杂氧化锌纳米粉末制备及其对PET的抗静电改性

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工程塑料应用 2024年第1期52卷 138-145页

作者：杨昕悦关丽涛杨崇岭华南农业大学材料与能源学院广州510642 广东轻工职业技术学院轻化工程系广州510300

采用化学共沉淀法制备了不同含量的Al单元掺杂氧化锌(AZO)纳米粉体,并将自制的纳米AZO粉末用于聚对苯二甲酸乙二酯(PET)制备,以提高PET的抗静电性能。利用X射线行射、扫描电子显微镜、热重分析、表面电阻测试仪等测试手段,探究了Al单元... 详细信息

采用化学共沉淀法制备了不同含量的Al单元掺杂氧化锌(AZO)纳米粉体,并将自制的纳米AZO粉末用于聚对苯二甲酸乙二酯(PET)制备,以提高PET的抗静电性能。利用X射线行射、扫描电子显微镜、热重分析、表面电阻测试仪等测试手段,探究了Al单元掺杂对AZO粉体结构、形貌及电性能的影响。在PET制备过程中加入AZO,探究AZO对PET抗静电性能的影响。结果表明:所制备的AZO纳米粉体为结晶度良好的六方纤锌矿结构。Al掺杂浓度影响AZO粉体形貌、晶体结构及电性能。随着Al掺杂浓度的增加,粉体的结晶质量降低,电性能先变好后变差,粉体尺寸变大,出现团聚、结块现象。适度的Al掺杂可使AZO电阻率达到最低,改善AZO的导电性能。在Al掺杂浓度为2%时,AZO纳米粉体的电性能最佳,电阻率为5.3×10^(4)Ω/m^(2)。使用制备的AZO纳米粉末作为导电填料能有效改善PET的抗静电性能,降低PET/AZO复合材料的表面电阻率,使材料由绝缘体转变为静电耗散体,抗静电性能增强。AZO填充量在0.005%时复合材料的表面电阻率最低,为1.16×10^(10)Ω/m^(2)。

关键词：化学共沉淀法掺杂改性纳米氧化锌 PET复合材料表面电阻率