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Expression of telomerase activity and oxidative stress in human hepatocellular carcinoma with cirrhosis

World Journal of Gastroenterology 2003年第8期9卷 1859-1862页

作者： Dao-YongLiu Zhi-HaiPeng Guo-QiangQiu chong-zhizhou DepartmentofGeneralSurgery ShanghaiNo.5People'sHospitalShanghai200240China DepartmentofGeneralSurgery shanghaiFirstPeople’sHospitalShanghai200080China

AIM: To study the expression and significance of telomerase activity and oxidative stress in hepatocellular carcinoma (HCC) with ***: In this study, TRAP-ELISA assay was used to determine telomerase activity in 21 cases of HCC as well as in 23 cases of hepatic cirrhosis. Malondialdehyde(MDA),glutathione S-transferase (GST) and total anti-oxidative capacity (T-AOC) were also examined in the same samples with human MDA, GST and T-AOC ***: Eighteen of 21 cases of HCC were found to have increased telomerase activity, whereas only three of the 23non-cancerous cirrhotic samples were found to have weak telomerase activity, and the difference was significant (P＜0.001). No significant difference in telomerase activity was detected according to different tumor size, tumor stage,histological grade, HBsAg, contents of albumin, bilirubin,ALT, AFP, r-GT and platelet. There were significant differences between HCC and cirrhosis in the expression of MDA, GST and T-AOC respectively. Telomerase activity correlated positively with the content of MDA (P＜0.05).CONCLUSION: Telomerase activation is the early event of carcinogenesis, which is not correlated with clinicopathological factors of HCC. The dysfunction of the anti-oxidative system is closely correlated with the progression from cirrhosis to hepatocellular carcinoma. Oxidative stress may contribute partly to telomerase activation.

关键词：肝细胞癌肝硬化氧化应激端粒酶

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Loss of heterozygosity on hromosome 1 in sporadic colorectal carcinoma

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World Journal of Gastroenterology 2004年第10期10卷 1431-1435页

作者： chong-zhizhou Guo-QiangQiu FangZhang LinHe Zhi-HaiPeng DepartmentofGeneralSurgery ShanghaiJiaotongUniversityFirstPeople'sHospitalShanghai200080China ShanghaiInstituteforBiologicalScience ChineseAcademyofSciencesShanghai200031China

AIM: Loss of heterozygosity (LOH) on tumor suppressor genes is believed to play a key role in carcinogenesis of colorectal cancer. When it occurs at a tumor suppressor gene locus with abnormal allele, neoplastic transformation happens. In this study, we analyzed the LOH at 21 loci on chromosome 1 in sporadic colorectal cancer to identify additional loci involved in colorectal ***: Twenty-one polymorphic micro-satellite DNA markers were analyzed with PCR both in 83 cases of colorectal cancer and in normal tissues. PCR products were eletrophoresed on an ABI 377 DNA sequencer. Genescan 3.1 and Genotype 2.1 software were used for LOH scanning and analysis. X^2 test was used to compare LOH frequency with clinicopathological data. P<0.05 was considered as statistically ***: The average LOH frequency of chromosome 1,short arm and long arm was 19.83%, 18.00% and 21.66%,respectively. The 2 highest LOH loci with a frequency of 36.54% and 32.50% were identified on DIS468 (1p36.33-p36.31) and DIS413 (1q31.3), respectively. On DIS2726 locus, LOH frequency of rectal cancer was 28.57% (6/21),which was higher than that of colon cancer (0.00%, 0/33) (P=0.002), suggesting that the mechanism of carcinogenisis was different in both ***: Putative tumor suppressor genes on chromosome 1 may relate to sporadic colorectal ***-suppressor-genes might locate on 1p36.33-36.31and/or 1q31.3.

关键词：零星结直肠癌杂合性缺失肿瘤病理学肿瘤抑制基因

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Detailed deletion mapping of loss of heterozygosity on 22q13 in sporadic colorectal cancer

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World Journal of Gastroenterology 2005年第11期11卷 1668-1672页

作者： Hai-TaoZheng Zhi-HaiPeng chong-zhizhou Da-PengLi Zhao-WenWang Guo-QiangQiu LinHe DepartmentofGeneralSurgery ShanghaiFirstPeopleHospitalShanghai200080China ShanghaiInstitutesforNutritionalSciences ChineseAcademyofScienceShanghai200031China

AIM: Both development and progression of malignancies occur as a multistep process, requiring the activation of oncogenes and the inactivation of several tumor suppressor genes. The loss of heterozygosity (LOH) of tumor suppressor genes is believed to play a key role in carcinogenesis of colorectal cancer (CRC). In this study, we analyzed the LOH of seven loci on chromosome 22q13 in an effort to identify candidate tumor suppressor genes involved in colorectal carcinogenesis. METHODS: Matched tumor and normal tissue DNA were analyzed by PCR using fluorescence-labeled polymorphic microsatellite markers in 83 CRC patients. PCR products were eletrophoresed and LOH was determined by calculating the peak height acquired through computer software. Comparisons between LOH frequency and clinicopathological features were performed by x2 test. P<0.05 was considered as statistical significance. RESULTS: The average LOH frequency of chromosome 22q13 was 28.38%. The highest LOH frequency was 64.71% on D22S1160 locus, and the lowest was 21.43% on D22S1141 locus. We detected two obvious minimal deletion regions: one between markers D22S1171 and D22S274, the other flanked by markers D22S1160 and D22S1149, each about 2.7 and 1.8 cm, respectively. None had lost in all informative loci. LOH frequency on D22S1171 is 50% on distal colon, which was higher than that on proximal one (P= 0.020); on D22S114 locus, none LOH event occurred in patients with liver metastasis, whilst 46.94% occurred in patients without liver metastasis (P= 0.008); on D22S1160 locus, LOH frequency in lymph nodes metastasis patients was 83.33%, which was much higher than 43.75% without lymph nodes metastasis ones (P= 0.016). There was no statistical significance between clinicopathological features and other loci. CONCLUSION: This study provides evidence of two minimal deletion regions, which may harbor putative tumor suppressor genes related to progression and metastasis in sporadic colorectal carcinoma on chromoso

关键词： Heterozygosity Chromosome 22 Sporadic colorectal cancer Gene mapping

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HPLC法测定奥沙利铂杂质B的研究

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中国药品标准 2012年第4期13卷 255-258页

作者：牛冲李军李涛徐志洲山东省药品检验所济南250101

目的:建立奥沙利铂及其制剂中杂质B(二水二胺环己烷合铂)的HPLC测定方法。方法:采用C18色谱柱;流动相为庚烷磺酸钠溶液(1.36 g磷酸二氢钾和1 g庚烷磺酸钠溶解于1 000 mL水中,用磷酸调pH值至3.0±0.05)-乙腈(83∶17);流量1.0 ***-1;检测... 详细信息

目的:建立奥沙利铂及其制剂中杂质B(二水二胺环己烷合铂)的HPLC测定方法。方法:采用C18色谱柱;流动相为庚烷磺酸钠溶液(1.36 g磷酸二氢钾和1 g庚烷磺酸钠溶解于1 000 mL水中,用磷酸调pH值至3.0±0.05)-乙腈(83∶17);流量1.0 ***-1;检测波长215 nm;柱温40℃。结果:杂质B在11.17～89.36 ng范围内线性关系良好(r=0.999 8),平均回收率为98.7%,RSD为0.5%(n=9),定量限为2.2 ng。结论:所建方法简便、准确,可用于奥沙利铂及其制剂中杂质B的检测。

关键词：高效液相色谱法奥沙利铂杂质有关物质二水二胺环己烷合铂

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脑梗死合并脑微出血患者血清MMP-9、IL-6、hs-CRP水平的研究

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中国医药科学 2018年第21期8卷 143-145,148页

作者：范道丰刘丽萍潘丽英陈丽萍陈银娟郑冲胡小红胡职舟陈衍贵福建医科大学附属龙岩第一医院神经内科福建龙岩364000 福建医科大学附属龙岩第一医院放射科福建龙岩364000

目的分析研究血清基质金属蛋白酶9(MMP-9)、白细胞介素6(IL-6)、超敏C反应蛋白(hs-CRP)与脑梗死合并脑微出血的关系。方法选择200例脑梗死患者,回顾性分析患者的临床资料,全部患者均给予磁敏感加权成像和头磁共振成像检查,根据磁敏感加... 详细信息

目的分析研究血清基质金属蛋白酶9(MMP-9)、白细胞介素6(IL-6)、超敏C反应蛋白(hs-CRP)与脑梗死合并脑微出血的关系。方法选择200例脑梗死患者,回顾性分析患者的临床资料,全部患者均给予磁敏感加权成像和头磁共振成像检查,根据磁敏感加权成像检查结果将全部200例患者分成非脑微出血组(150例)和脑微出血组(50例)。对患者的临床资料进行记录;对血清MMP-9、IL-6与hs-CRP水平进行检查,选择Logistic回归分析MMP-9、IL-6、hs-CRP与脑微出血的相互关系。结果脑微出血组患者的血清MMP-9、IL-6与hs-CRP水平显著高于非脑微出血组患者,差异有统计学意义(P <0.05)。Logistic多元回归分析发现,调整了传统危险因素、性别以及年龄的影响,血清MMP-9、IL-6与hs-CRP均为发生脑微出血的危险因素。结论血清MMP-9、IL-6与hs-CRP水平与脑微出血的发生密切相关,临床中应加以关注和重视。

关键词：基质金属蛋白酶9 白细胞介素6 超敏C反应蛋白脑梗死脑微出血

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