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Cloning and expression analysis of the chloroplast fructose-1,6-bisphosphatase gene from Pyropia haitanensis

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Acta Oceanologica Sinica 2014年第4期33卷 92-100页

作者： XIAO Haidong CHEN Changsheng XU Yan JI Dehua XIE chaotian Fisheries College Jimei University

Fructose-1,6-bisphosphatase（FBPase） is one of the key enzymes in Calvin circle and starch biosynthesis. In this study, the full-length of cpFBPase gene from Pyropia haitanensis was cloned by using rapid amplification of cDNA ends（RACE） technology. The nucleotide sequence of PhcpFBPase consists of 1 400 bp, including a 5′ untranslated region（UTR） of 92 bp, a 3′？UTR of 69 bp, and an open reading frame（ORF） of 1 236 bp, which can be translated into a 412-amino-acid putative peptides with a molecular weight of 44.3 kDa and a theoretical pI of 5.23. Multiple sequence alignment indicated that the protein belonged to the chloroplast FBPase enzyme. Phylogenetic analysis showed that the protein assembled with the cpFBPase of a thermal tolerant unicellular red micro-algae Galdieria sulphuraria. Expression patterns analyzed by qRT-PCR revealed that the expression of PhcpFBPase gene in the thallus phage was 7-fold higher than in the conchocelis phage, which suggested the different mechanisms of inorganic carbon utilization among the different life phages of P. haitanensis. And the different response modes of PhcpFBPase mRNA levels to high temperature and desiccation stress indicated that PhcpFBPase played an important role in responsing to abiotic stress.

关键词： Pyropia haitanensis fructose-1 6-bisphosphatase gene cloning qRT-PCR RACE

Validation of housekeeping genes as internal controls for studying the gene expression in Pyropia haitanensis(Bangiales, Rhodophyta) by quantitative real-time PCR

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Acta Oceanologica Sinica 2014年第9期33卷 152-159页

作者： LI Bing CHEN Changsheng XU Yan JI Dehua XIE chaotian Fisheries College Jimei University

Pyropia haitanensis is an economically important mariculture crop in China and has a high research value for several life phenomena, for example environmental tolerance. To explore the mechanisms underlying these characteristics, gene expression has been investigated at the whole transcriptome level. Gene expression studies using quantitative real-time PCR should start by selecting an appropriate internal control gene; therefore, the absolute expression abundance of six housekeeping genes （18S rRNA （18S）, ubiquitin-conju-ating enzyme （UBC）, actin （ACT）, β-tubulin （TUB）, elongation factors 2 （EF2）, and glyceraldehyde-3-phos- phate dehydrogenase （GAPDH） examined by the quantitative real-time PCR in samples corresponding to different strains, life-cycle stages and abiotic stress treatments. Their expression stabilities were assessed by the comparative cycle threshold （Ct） method and by two different software packages： geNorm and NormFinder. The most stable housekeeping gene is UBC and the least stable housekeeping is GADPH. Thus, it is proposed that the most appropriate internal control gene for expression analyses in P. haitanensis is UBC. The results pave the way for further gene expression analyses of different aspects of P. haitanensis biology including different strains, life-history stages and abiotic stress responses.

关键词： Pyropia haitanensis quantitative real-time PCR internal control genes gene expression

Superoxide dismutase genes in Pyropia haitanensis:molecular cloning,characterization and mRNA expression

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Acta Oceanologica Sinica 2016年第6期35卷 101-111页

作者： JI Dehua XU Yan XIAO Haidong CHEN Changsheng XU Kai XIE chaotian Fisheries College Jimei University

Superoxide dismutase（SOD,EC 1.***.1.1） is the first and most important line of cellular defense against oxidative *** this study,based on unigene sequences of ***,three full-length Ph SOD genes were obtained by RACE technology,and named Ph MSD,Ph CSD1 and Ph *** full-length c DNAs of these genes comprised973,1 029 and 954 nucleotides,*** c DNAs encoded proteins of 224,134 and 216 amino acids,with isoelectric points of 5.75,4.65 and 10.74,*** on their conserved motifs and phylogenetic tree analysis,the three Ph SODs were divided into two SOD types：Ph MSD is a Mn-SOD,and Ph CSD1 and Ph CSD2 are Cu Zn-SODs.q PCR was used to measure the expression of the three Ph SOD genes in different life phases of ***,and under different periods of high-temperature stress and different levels of *** the different life phases of ***,the expression levels of the three Ph SODs were all significantly higher in the thallus than in the *** high-temperature and desiccation stress,the expression levels of Ph CSD1 and Ph CSD2 were highly induced by the O2-content,but the expression level of Ph MSD was highly depressed by high temperature stress and showed no significant change during desiccation.

关键词： superoxide dismutase gene cloning desiccation stress high-temperature stress qPCR

Effects of Low Temperature Stress on Germination and Physiological Characteristics of Different Sweet Maize Varieties

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Plant Diseases and Pests 2024年第1期15卷 1-3,6页

作者： Zhenxing WU Jianjian CHEN Tingzhen wang Guihua LU Institute of Maize and Featured Upland Crops Zhejiang Academy of Agricultural SciencesDongyang 322100China

[Objectives]The paper was to explore the effects of low temperature stress on germination and physiological characteristics of different sweet maize varieties.[Methods]Taking Taitian 264,Zhexuetian 1 and chaotian 4 as the research objects,the changes in germination potential,germination index,plant height,biomass,and antioxidant enzyme activity of maize seeds were studied under optimal temperature conditions(25℃)and low temperature stress conditions(10℃).[Results]Under 10℃stress,the germination rate and germination index of Taitian 264 were higher than that of Zhexuetian 1 and chaotian *** low temperature stress,Taitian 264 exhibited the least reduction in height and biomass,while Zhexuetian 1 had the most ***,the SOD and POD activities of Taitian 264 were higher than that of Zhexuetian 1 and chaotian 4 under both temperature conditions,while the MDA content of Taitian 264 was *** 264 showed strong germination ability against low temperature stress.[Conclusions]This study provides a basis for timely sowing practices of sweet maize in agricultural production.

关键词： Sweet maize Low temperature stress Germination Antioxidant enzyme

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废弃矿井酸性废水治理和风险管控研究

四川环境 2024年第2期43卷 65-71页

作者：张娇谢腾蛟王晓龙廖禄云钟昌茂王承俊四川省地质工程勘察院集团有限公司成都610000 四川省天源环保股份有限公司成都610036 广元市朝天生态环境局四川广元628000

在矿产资源开采过程中,矿井不仅会改变原有地下水补径排系统,同时矿井内流出的地下水携带重金属离子、硫酸根离子及悬浮物等将对周围环境造成严重污染。对此,以四川某废弃煤矿为研究对象,提出废弃矿井酸性废水治理技术,该技术基于对酸... 详细信息

在矿产资源开采过程中,矿井不仅会改变原有地下水补径排系统,同时矿井内流出的地下水携带重金属离子、硫酸根离子及悬浮物等将对周围环境造成严重污染。对此,以四川某废弃煤矿为研究对象,提出废弃矿井酸性废水治理技术,该技术基于对酸性矿井水成因的系统性分析,对具有不同涌水特征的矿井分别采取矿井封隔和多级次曝气沉淀风险管控处理技术,进而促进酸性矿井水向自我修复方向演化,以实现地表水与地下水污染的治理。采用该技术后,矿区内地下水水质和水位逐渐恢复,地下水生态环境逐渐改善,2#矿井pH值由2.73增大到7.65,水的酸碱性由酸性变为中性,同时铁含量显著降低,含量值由最初的970mg/L降低至62.6mg/L,下降了93.5%;而4#矿井的水位在监测120天后上升了约50m且保持相对稳定。同时,通过跌水、曝气等风险管控措施后,进入下游流域的污染物浓度下降,特别是地表水中铁含量及浊度显著降低,生态环境逐渐得到改善。该技术成功解决了如何实现酸性矿井涌水经济、有效和可持续治理难题,为矿区水污染修复和风险管控提供参考。

关键词：废弃矿区地下水污染污染修复风险管控

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大型海藻育种技术研究进展及其应用

水产学报 2023年第11期47卷 44-57页

作者：王文磊徐燕纪德华谢潮添集美大学水产学院福建省水产生物育种与健康养殖工程研究中心福建省发展和改革委员会集美大学农业农村部东海健康养殖重点实验室集美大学水产学院海水养殖生物育种全国重点实验室

大型海藻栽培业是全球最活跃的渔业产业之一，近二十年增幅是整体渔业增幅的两倍以上，发展前景十分广阔。其中，中国大型海藻产量占全球总产量的59%，海带、紫菜、裙带菜、龙须菜以及羊栖菜产量均排名世界第一。水产种业是水产养殖业... 详细信息

大型海藻栽培业是全球最活跃的渔业产业之一，近二十年增幅是整体渔业增幅的两倍以上，发展前景十分广阔。其中，中国大型海藻产量占全球总产量的59%，海带、紫菜、裙带菜、龙须菜以及羊栖菜产量均排名世界第一。水产种业是水产养殖业的“芯片”和整个产业链的源头，大型海藻产量99%来自人工栽培，这更体现了新品种对产业的贡献度和重要性。但目前经过审定的大型海藻新品种仅有24个，约占海水养殖新品种的18%，与其产量占比并不匹配。为此，本文介绍了大型海藻产业的特点、近60年育种技术进展和育种成果，并针对大型海藻育种技术发展现状提出了相关建议，以期为大型海藻育种研究提供一些思路。

关键词：大型海藻新品种育种技术

Characterization and comparative profiting of the microRNA in two generations of Porphyra haitanensis Chang et Zheng（Bangiales,Rhodophyta）

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Characterization and comparative profiting of the microRNA i...

大学生就业管理系统工程的结构模型与优化控制

2011年中国水产学会学术年会

作者： Gaoshang Mei,chaotian Xie,Changsheng Chen ,YanXul,DehuaJi

＜正＞Porphyra,one of the most important marine crops in Asia,possesses two heteromorphic life *** studies have found that the expressions of the majority of genes of Porphyra are ***,there is limited knowledge of the regulation expression of these phase-related ***（miRNAs） are a class of endogenous small RNAs that play essential regulatory roles in gene *** gain an insight into the characteristics and expression patterns of miRNAs and to investigate their roles phase-specific expression phases,we used next generation high-throughput sequencing technology to identify miRNAs of *** on a large scale.

关键词： Porphyra haitanensis different life phases microRNA

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南方经济 2016年第5期45卷 87-96页

作者：魏伟陈潮填广东技术师范学院广东技术师范学院计算机科学学院

本文把大学生就业管理看成一项系统工程,首先利用系统科学的观点、原理和方法,从需求侧数量、供给侧结构、服务功能、政策环境等方面对我国大学生就业的现状、问题及原因进行系统分析;然后从大学生就业管理的对象、内容和过程的角度构... 详细信息

本文把大学生就业管理看成一项系统工程,首先利用系统科学的观点、原理和方法,从需求侧数量、供给侧结构、服务功能、政策环境等方面对我国大学生就业的现状、问题及原因进行系统分析;然后从大学生就业管理的对象、内容和过程的角度构建大学生就业管理系统工程的设计结构模型、分析框架模型和控制结构模型;最后利用系统工程理论提出"拓宽供给,分流需求,柔性就业,动态平衡"的宏观调控策略及就业管理系统工程的优化控制措施。

关键词：大学生就业管理系统工程结构模型控制措施

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论科学发展职业教育应解决的思想认识问题

教育与职业 2006年第32期 11-12页

作者：陈潮填广东技术师范学院计算机系系

“十一五”期间将是我国职业教育的大发展时期。能否有效促进我国各地各级职业教育的科学发展，首先取决于各级领导和职教工作者，乃至社会对职业教育的思想认识。本文从职业教育的发展形势、社会作用、培养目标、人才待遇、学校特色等... 详细信息

“十一五”期间将是我国职业教育的大发展时期。能否有效促进我国各地各级职业教育的科学发展，首先取决于各级领导和职教工作者，乃至社会对职业教育的思想认识。本文从职业教育的发展形势、社会作用、培养目标、人才待遇、学校特色等方面进行了论述，期望能够引起进一步的探讨，并借此提高人们对职业教育规律及其重要性的认识。

关键词：职业教育科学发展思想认识