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Current Medical Science 2023年第1期43卷 35-47页

作者： Ning JI chong-guang wu Xiao-die WANG Zhi-xue SONG Pei-yuan wu Xin LIU Xu FENG Xiang-mei ZHANG Xiu-fang WANG Zhan-jun LV Department of Genetics Hebei Medical UniversityHebei Key Lab of Laboratory AnimalShijiazhuang 050017China Research Center Fourth Hospital of Hebei Medical UniversityShijiazhuang 050011China

Objective:To investigate whether human short interspersed nuclear element antisense RNA(Alu antisense RNA;Alu asRNA)could delay human fibroblast senescence and explore the underlying ***:We transfected Alu asRNA into senescent human fibroblasts and used cell counting kit-8(CCK-8),reactive oxygen species(ROS),and senescence-associated beta-galactosidase(SA-β-gal)staining methods to analyze the anti-aging effects of Alu asRNA on the *** also used an RNA-sequencing(RNA-seq)method to investigate the Alu asRNA-specific mechanisms of *** examined the effects of KIF15 on the anti-aging role induced by Alu *** also investigated the mechanisms underlying a KIF15-induced proliferation of senescent human ***:The CCK-8,ROS and SA-β-gal results showed that Alu asRNA could delay fibroblast ***-seq showed 183 differentially expressed genes(DEGs)in Alu asRNA transfected fibroblasts compared with fibroblasts transfected with the calcium phosphate transfection(CPT)*** KEGG analysis showed that the cell cycle pathway was significantly enriched in the DEGs in fibroblasts transfected with Alu asRNA compared with fibroblasts transfected with the CPT ***,Alu asRNA promoted the KIF15 expression and activated the MEK-ERK signaling ***:Our results suggest that Alu asRNA could promote senescent fibroblast proliferation via activation of the KIF15-mediated MEK-ERK signaling pathway.

关键词： senescent fibroblast cell proliferation Alu antisense RNA KIF15 gene expression MEK-ERK signaling pathway cell cycle

Alu antisense RNA ameliorates methylglyoxal-induced human lens epithelial cell apoptosis by enhancing antioxidant defense

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International Journal of Ophthalmology(English edition) 2023年第2期16卷 178-190页

作者： Pei-Yuan wu Ning Ji chong-guang wu Xiao-Die Wang Xin Liu Zhi-Xue Song Murad Khan Suleman Shah Ying-Hua Du Xiu-Fang Wang Li-Fang Yan Department of Genetics Hebei Medical UniversityHebei Key Lab of Laboratory AnimalShijiazhuang 050017Hebei ProvinceChina Department of Ophthalmology Beijing Tiantan HospitalCapital Medical UniversityBeijing 100070China

AIM:To determine whether an antisense RNA corresponding to the human Alu transposable element(Aluas RNA)can protect human lens epithelial cells(HLECs)from methylglyoxal-induced ***:Cell counting kit-8(CCK-8)and 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)assays were used to assess HLEC *** viability/death was detected using a Calcein-AM/PI double staining kit;the annexin V-FITC method was used to detect HLEC *** cytosolic reactive oxygen species(ROS)levels in HLECs were determined using a reactive species assay *** levels of malondialdehyde(MDA)and the antioxidant activities of total-superoxide dismutase(T-SOD)and glutathione peroxidase(GSH-Px)were assessed in HLECs using their respective ***-q PCR and Western blotting were used to measure m RNA and protein expression levels of the ***:Aluas RNA rescued methylglyoxal-induced apoptosis in HLECs and ameliorated both the methylglyoxalinduced decrease in Bcl-2 m RNA and the methylglyoxalinduced increase in Bax m *** addition,Aluas RNA inhibited the methylglyoxal-induced increase in Alu sense RNA *** RNA inhibited the production of ROS induced by methylglyoxal,restored T-SOD and GSHPx activity,and moderated the increase in MDA content after treatment with *** RNA significantly restored the methylglyoxal-induced down-regulation of Nrf2 gene and antioxidant defense genes,including glutathione peroxidase,heme oxygenase 1,γ-glutamylcysteine synthetase and quinone oxidoreductase *** RNA ameliorated methylglyoxal-induced increases of the m RNA and protein expression of Keap1 that is the negative regulator of ***:Aluas RNA reduces apoptosis induced by methylglyoxal by enhancing antioxidant defense.

关键词： human Alu antisense RNA human lens epithelial cells methylglyoxal toxicity antioxidant defense apoptosis

Extending the detection limit:innovations in infrared quantum dot photodetectors reaching up to 18μm

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Light(Science & Applications) 2024年第8期13卷 1445-1447页

作者： chong wu Wang Qi Jie Wang Centre for OptoElectronics and Biophotonics School of Electrical and Electronic EngineeringNanyang Technological UniversitySingapore 639798Singapore Centre for Disruptive Photonic Technologies School of Physical and Mathematical SciencesNanyang Technological UniversitySingaporeSingapore

A regrowth method was used to synthesize large-sized colloidal quantum dots(CQDs).With the assistance of doping engineering,the synthesized CQD detectors demonstrate exceptional long-wavelength infrared detection performance,reaching up to 18μm,significantly extending the spectral response limit for CQD-based infrared *** detectors also achieve a reasonably high detectivity of 6.6×10^(8)Jones.

关键词： limit quantum sized

Conduction-convection coupled heat transfer around a hollow cylinder under different buoyancy forces

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Chinese Journal of Aeronautics 2024年第4期37卷 216-228页

作者： Wanyu ZHANG Jingyi wu guang YANG Institute of Refrigeration and Cryogenics Shanghai Jiao Tong UniversityShanghai 200240China

In order to clarify the effect of a buoyancy force on conduction–convection coupled heat transfer in a hollow cylinder, the flow and thermal characteristics were analyzed using an RNG k-ε turbulence model. The Reynolds number was fixed at 1.014 × 10^(6), and the Rayleigh number varied from 1.122 × 10^(10)to 1.088 × 10^(11). Results have shown that, when considering the effect of an opposed buoyancy force, increasing the Rayleigh number has a positive impact on the rate of change and uniformity of the cylinder temperature. The temperature distributions along the axial and circumferential directions are similar for different Rayleigh numbers, but extreme values *** the axial direction, the maximum temperature is obtained at the interface between the variable-diameter part and the constant-diameter part. The maximum dimensionless temperature value decreases to 0.12 when the Rayleigh number increases to 1.088 × 10^(11). Along the circumferential direction, the temperature distribution is affected by the buoyancy force, which results in the temperature of the upper part being higher than that of the lower part. After nondimensionalization of the temperature and time, a correlation was proposed to illustrate the transient heat transfer process quantitatively. The standard deviation of the maximum relative temperature, representing the temperature uniformity, was also calculated. It was found that the difference in the direction of the buoyancy force made a huge difference. Compared with the opposed buoyancy force, the maximum dimensionless temperature is almost two times higher with an assisted buoyancy force. Similarly, the heat transfer coefficient with an assisted buoyancy force is half of that with an opposed buoyancy force. Overall, an assisted buoyancy force plays a negative role in terms of thermal characteristics. The flow field around the hollow cylinder was also illustrated to reveal the mechanism of the buoyancy force on magnitude and direction aspects

关键词： Buoyancy Conduction-convection heat transfer Transient analysis Hollow cylinder Non-dimensionalization

Three-dimensional morphological and fluorescent imaging of zebrafish with a continuous-rotational light-sheet microscope

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Journal of Innovative Optical Health Sciences 2024年第2期17卷 59-66页

作者： Dongmin Zhang guang Yang Yao Tan chong Chen Jie Zhang Hui Li School of Biomedical Engineering(Suzhou)Division of Life Sciences and Medicine University of Science and Technology of China Hefei 230041 P.R.China Suzhou Institute of Biomedical Engineering and Technology Chinese A cademy of Sciences Suzhou Jiangsu 215163P.R.China

Light-sheet fluorescence microscopy(LSFM)has been widely used to image the three-dimensional(3D)structures and functions of various millimeter-size bio-specimen such as ***,the sample adsorption and scattering cause shading of the light-sheet illumination,preventing the even 3D image of thick ***,we report a continuous-rotational light-sheet microscope(CR-LSM)that enables simultaneous 3D bright-field and fluorescence *** a high-accuracy rotational stage,CR-LSM records the outline projections and the fluorescent images of the sample at multiple rotation ***,3D morphology and fluorescent structure were reconstructed with a developed *** CR-LSM,zebrafish’s whole-fish contour and blood vessel structures were obtained simultaneously.

关键词： Light-sheet microscope zebrafish blood vessels morphology

Complications and comorbidities associated with antineoplastic chemotherapy:Rethinking drug design and delivery for anticancer therapy

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Acta Pharmaceutica Sinica B 2024年第7期14卷 2901-2926页

作者： Xiaoman Mao Shuang wu Dandan Huang chong Li College of Pharmaceutical Sciences Southwest UniversityChongqing 400715China Medical Research Institute Southwest UniversityChongqing 400715China School of Pharmaceutical Sciences Southern Medical UniversityGuangzhou 510515China

Despite the considerable advancements in chemotherapy as a cornerstone modality in cancer treatment,the prevalence of complications and pre-existing diseases is on the rise among cancer patients along with prolonged survival and aging *** relationships between these disorders and cancer are intricate,bearing significant influence on the survival and quality of life of individuals with cancer and presenting challenges for the prognosis and outcomes of ***,we review the prevailing complications and comorbidities that often accompany chemotherapy and summarize the lessons to learn from inadequate research and management of this scenario,with an emphasis on possible strategies for reducing potential complications and alleviating comorbidities,as well as an overview of current preclinical cancer models and practical advice for establishing bio-faithful preclinical models in such complex context.

关键词： Complications Comorbidities Chemotherapy Preclinical cancer models Drug design Drug delivery

Genomic insights into adaptation to bipedal saltation and desert-like habitats of jerboas

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Science China(Life Sciences) 2024年第9期67卷 2003-2015页

作者： Simin Chai Yujie chong Daiqing Yin Qiang Qiu Shixia Xu guang Yang Southern Marine Science and Engineering guangdong Laboratory(guangzhou) Guangzhou 511458China Jiangsu Key Laboratory for Biodiversity and Biotechnology School of Life SciencesNanjing Normal UniversityNanjing 210023China School of Ecology and Environment Northwestern Polytechnical UniversityXi’an 710072China

Jerboas is a lineage of small rodents displaying atypical mouse-like morphology with elongated strong hindlimbs and short *** have evolved obligate bipedal saltation and acute senses,and been well-adapted to vast desert-like *** a newly sequenced chromosome-scale genome of the Mongolian five-toed jerboa(Orientallactaga sibirica),our comparative genomic analyses and in vitro functional assays showed that the genetic innovations in both protein-coding and non-coding regions played an important role in jerboa morphological and physiological ***-specific amino acid substitutions,and segment insertions/deletions(indels)in conserved non-coding elements(CNEs)were found in components of proteoglycan biosynthesis pathway(XYLT1 and CHSY1),which plays an important role in limb ***,we found specific evolutionary changes functionally associated with energy or water metabolism(e.g.,specific amino acid substitutions in ND5 and indels in CNEs physically near ROR2)and senses(e.g.,expansion of vomeronasal receptors and the FAM136A gene family)in *** dual-luciferase reporter assay verified that some of the CNEs with jerboa-specific segment indels exerted a significantly different influence on luciferase activity,suggesting changes in their regulatory function in *** results revealed the potential molecular mechanisms underlying jerboa adaptation since the divergence from the Eocene-Oligocene transition,and provided more resources and new insights to enhance our understanding of the molecular basis underlying the phenotypic diversity and the environmental adaptation of mammals.

关键词： jerboa adaptive evolution comparative genomics conserved non-coding elements bipedal saltation

State estimation for delayed switched positive systems: delayed radius approach

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Science China(Information Sciences) 2024年第8期67卷 190-205页

作者： Weizhong CHEN Zhongyang FEI Xudong ZHAO Zheng-guang wu School of Electronics and Information Xi'an Polytechnic University Research Institute of Intelligent Control and Systems Harbin Institute of Technology Key Laboratory of Intelligent Control and Optimization for Industrial Equipment of Ministry of Education Dalian University of Technology School of Control Science and Engineering Dalian University of Technology State Key Laboratory of Industrial Control Technology Institute of Cyber-Systems and ControlZhejiang University

In this paper, an interval estimation scheme is developed for delayed switched positive systems（DSPS） with mode-dependent average dwell time switching. A lossless zonotopic estimation approach is proposed for the delayed intersection zonotope with the positive generator matrix. First, considering the existence of asynchronism between the system mode and the correction matrix mode, the mismatched intersection zonotope is constructed for DSPS to verify the consistency between the system model and ***, by utilizing the introduced radius definitions, the ?∞performance is addressed to optimize the size of delayed intersection zonotopes. Subsequently, we present a joint-design approach of switching signals and the mode-dependent correction matrix by constructing positive generator matrix-based delayed radius functions. Furthermore, guaranteed nonnegative state bounds are derived for the considered DSPS based on the proposed lossless zonotopic estimation criteria. Finally, detailed simulations are conducted to validate the feasibility and superiority of the developed methods.

关键词： delayed switched positive systems interval estimation asynchronous correction matrix zonotopes mode-dependent average dwell time

Effect of Mn addition on microstructure and mechanical properties of GX40CrNiSi25-12 austenitic heat resistant steel

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China Foundry 2024年第3期21卷 205-212页

作者： Guan-yu Jiang Meng-wu wu Xiao-guang Yang Hui Wang Yu-yuan Zhu Hubei Key Laboratory of Advanced Technology for Automotive Components Wuhan University of TechnologyWuhan 430070China Hubei Longzhong Laboratory Xiangyang 441000HubeiChina Wescast Industries(China)Co. Ltd.Wuhan 430100China

Three types of steels were designed on the basis of GX40CrNiSi25-12 austenitic heat resistant steel by adding different Mn contents(2wt.%,6wt.%,and 12wt.%).Thermodynamic calculation,microstructure characterization and mechanical property tests were conducted to investigate the effect of Mn addition on the microstructure and mechanical properties of the austenitic heat resistant *** show that the matrix structure in all the three types of steels at room temperature is completely *** NbC and M_(23)C_(6)precipitate at grain boundaries of austenite *** the increase of Mn content,the number of carbides increases and their distribution becomes more *** the Mn content increases from 1.99%to 12.06%,the ultimate tensile strength,yield strength and elongation increase by 14.6%,8.0%and 46.3%,*** improvement of the mechanical properties of austenitic steels can be explained by utilizing classic theories of alloy strengthening,including solid solution strengthening,precipitation strengthening,and grain *** increase in alloy strength can be attributed to solid solution strengthening and precipitation strengthening caused by the addition of *** improvement of the plasticity of austenitic steels can be explained from two aspects:grain refinement and homogenization of precipitated phases.

关键词： austenitic heat resistant steel manganese microstructure mechanical properties

Regulating the crystal phase of bismuth-based semiconductors for promoted photocatalytic performance

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Science China Chemistry 2024年第6期67卷 1839-1864页

作者： Zhaohui wu Mei-Chi chong Shiying Zhang Junshan Li Yongfa Zhu Hunan Key Laboratory of Applied Environmental Photocatalysis Changsha UniversityChangsha410022China Department of Chemistry Tsinghua UniversityBeijing100084China Institute for Advanced Study Chengdu UniversityChengdu610106China

Numerous bismuth-based semiconductors(BBSs)with sophisticated and desirable structures used as photocatalysts for efficient photocatalytic degradation of water organic contaminants have attracted considerable ***,regulating the crystal phases and phase transition of BBSs for promoted photocatalytic performance is ***,the unique crystal structure and band structure features of each typical BBSs,and the vital roles on phase controlling of each phase were systematically presented based on the classification of ***,the critical factors for the phase transition of BBSs and intrinsic driving forces endowed by phases of BBSs for enhanced photocatalytic performance of organic contaminants removal were also *** review will provide systematical guidelines and horizons for regulating the crystal phase and phase transition of BBSs,promoting photocatalytic degradation and mineralization of organic contaminants.

关键词： bismuth-based semiconductors phase regulation photocatalytic performances organic contaminants