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Association of Cytokines with Alanine Aminotransferase, Hepatitis B Virus Surface Antigen and Hepatitis B Envelope Antigen Levels in Chronic Hepatitis B

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Chinese Medical Journal 2018年第15期131卷 1813-1818页

作者： Ming-Hui Li Yao Lu Lu Zhang Xing-Yue Wang chong-Ping Ran Hong-Xiao Hao Dan Zhang Xiao-Jing Qu Ge Shen Shu-Ling Wu Wei-Hua Cao Tian-Lin Qi Ru-Yu Liu Lei-Ping Hu Min Chang Wen-Hao Hua Shun-Ai Liu Gang Wan Yao Xie Liver Diseases Center Beijing Ditan Hospital Capital Medical University Beijing 100015 China Beijing Key laboratory of Emerging Infectious Diseases Institute of Infectious Diseases Beijing Ditan Hospital Capital Medical University Beijing 100015 China Clinical Test Center Beijing Ditan Hospital Capital Medical University Beijing 100015 China Ming-Hui Li and Yao Lu contributed equally to this work

Background： Cytokines play an important role in occorrence and recovery of hepatitis B virus （HBV） infection. The aim of this study was to investigate the changes ofcytokines concentration and its correlation to alanine aminotransferase （ALT）, HBV deoxyribonucleic acid （HBV-DNA）, hepatitis B envelope antigen （HBeAg）, and HBV surface antigen （HBsAg） in the development of chronic hepatitis B （CHB）. Methods： Thirteen healthy individuals （HI）, 30 chronic HBV-infected patients in immune tolerant （IT） phase, and 55 CHB patients were enrolled between August 2015 and May 2017. The peripheral blood samples were collected from all individuals. Tile levels of interferon （IFN）-α2, interleukin （il）-10, transforming growth factor （TGF）-β1, HBV-DNA, HBsAg, and HBeAg and liver function were measured. The quantitative determinations of cytokines levels, including I FN-ct2, il-10, and TGF-[31 were performed using Luminex multiplex technology. The correlation of cytokines to ALT, HBV-DNA, HBsAg, and HBeAg was analyzed by linear regression analysis. Results： IFN-ct2 levels were similar between HI and IT groups （15.35 [5.70, 67.65] pg/ml vs. 15.24 [4.07, 30.73] pg/ml, Z = -0.610, P - 0.542）, while it elevated significantly in CH B group （35.29 [ 15.94, 70.15] pg/ml vs. 15.24 [4.07, 30.73] pg/ml; Z = -2.522, P = 0.012）. Compared with HI group （3.73 [2.98, 11.92] pg/ml）, il-10 concentrations in IT group （5.02 [2,98, 10.11] pg/ml）, and CHB group （7.48 [3. I 0, 18.00] pg/ml） slightly increased （X^2 = 2.015, P - 0.365）, and there was no significant difference between IT and CHB group （Z =- 1.419, P = 0.156）. The TGF-β1 levels among HI （3.59 ±0.20 pg/ml）, IT （3.62 ±0.55 pg/ml）, and CHB groups （3.64±0.30 pg/ml） were similar （X^2=2.739, P = 0.254）. In all chronic HBV-infected patients （including patients in IT and CHB groups）, the elevation of IFN-α2 level was significantly associated with ALT level （β = 0.389, t = 2.423, P = 0.018）, and was also negatively correlated to HBV-DNA load （β = -0.35

关键词： Chronic Hepatitis B Hepatitis B Virus Deoxyribonucleic Acid Hepatitis B Virus Surface Antigen Intertferon-α2: lnterleukin- 10

油酸-内毒素序贯致新生大鼠急性肺损伤il-18水平的变化

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南京医科大学学报（自然科学版） 2006年第10期26卷 941-943,F0003页

作者：史彧王崇伟南京医科大学附属南京儿童医院呼吸科南京210008

目的:探讨油酸-内毒素序贯致伤新生大鼠的il-18水平,在急性肺损伤中的作用。方法:采用油酸加内毒素脂多糖腹腔注射新生大鼠致肺损伤模型,在油酸致伤后4h实施内毒素第2次致伤,收集血浆检测il-18水平,同时设立单剂量内毒素致伤组和生理盐... 详细信息

目的:探讨油酸-内毒素序贯致伤新生大鼠的il-18水平,在急性肺损伤中的作用。方法:采用油酸加内毒素脂多糖腹腔注射新生大鼠致肺损伤模型,在油酸致伤后4h实施内毒素第2次致伤,收集血浆检测il-18水平,同时设立单剂量内毒素致伤组和生理盐水为对照组,每组12只。结果:油酸-内毒素致伤新生大鼠血浆il-18水平均显著高于单剂量内毒素致伤组和生理盐水对照组(Pil-18水平显著高于单剂量内毒素致伤组和生理盐水对照组,提示致炎因子il-18可能在急性肺损伤的发生和发展中起重要的作用。

关键词：油酸内毒素新生大鼠白细胞介素-18 急性肺损伤

血清趋化因子白细胞介素8对慢性阻塞性肺疾病急性加重的预测价值

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第二军医大学学报 2018年第5期39卷 480-487页

作者：张景熙范韵鑫白冲海军军医大学(第二军医大学)长海医院呼吸与危重症医学科上海200433

目的探讨血清趋化因子白细胞介素(il)-8对慢性阻塞性肺疾病(COPD)急性加重的预测价值。方法纳入2015年9月至2016年5月在我院初诊或未进行规范化治疗的急性加重期COPD患者112例,根据血清il-8水平将其分为高il-8组(≥62 pg/m L)和低il-8组... 详细信息

目的探讨血清趋化因子白细胞介素(il)-8对慢性阻塞性肺疾病(COPD)急性加重的预测价值。方法纳入2015年9月至2016年5月在我院初诊或未进行规范化治疗的急性加重期COPD患者112例,根据血清il-8水平将其分为高il-8组(≥62 pg/m L)和低il-8组(il-8、il-6、肿瘤坏死因子α(TNF-α)、超氧化物歧化酶水平和呼出气一氧化氮以及肺功能检查指标。随访1年,记录患者自随访开始至第1次出现中重度急性加重的时间及随访期间急性加重次数。血清il-8和各变量间的单因素分析采用Spearman相关分析,用Cox回归分析两组患者急性加重的相对危险度(RR),用Kaplan-Meier曲线分析随访12个月两组无急性加重患者所占比例的差异和风险比(HR)。结果高、低il-8组患者分别为47例和65例,il-8水平[中位数(下四分位数,上四分位数)]分别为170.00(111.00,472.00)pg/m L和22.40(7.90,34.45)pg/m L。高il-8组过敏性鼻炎患者比例高于低il-8组[51.06%(24/47)vs 10.77%(7/65),Pil-8组(24.81±5.10 vs 19.38±4.27,Pil-8组[1.5(1.0,2.5)vs 1.0(0.0,1.5),Pil-8组用力呼气中期流速(FEF25%-75%)、FEF25%-75%占预计值的百分比、用力呼气75%用力肺活量流速(FEF75%)、FEF75%占预计值的百分比均低于低il-8组[(0.73±0.55)L/min vs(1.26±1.15)L/min、(23.89±16.64)%vs(35.21±26.88)%、(0.32±0.19)L/min vs(0.57±0.53)L/min、(25.32±13.27)%vs(39.97±29.42)%,P均il-8水平与一氧化碳弥散量占预计值的百分比(DLCO%Pred)呈负相关(r=-0.402 1,P=0.001 8),且高il-8组DLCO%Pred低于低il-8组[(51.52±26.41)%vs(72.98±18.70)%,P=0.029]。随时间累积高il-8组急性加重的风险高于低il-8组,RR为3.750(95%CI:1.200~11.716,P=0.029);高il-8组距第1次发生急性加重的中位时间较低il-8组短,HR为3.066(95%CI:1.053~8.927,P=0.039)。结论血清il-8水平增高对COPD急性加重具有一定预测价值,可能与COPD急性加重患者合并过敏体质和小气道功能、弥散功能受损加重有关。

关键词：慢性阻塞性肺疾病急性加重趋化因子类白细胞介素8

Plasmacytoid Dendritic Cell Function and Cytokine Network Profiles in Patients with Acute or Chronic Hepatitis B Virus Infection

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Chinese Medical Journal 2018年第1期131卷 43-49页

作者： Ming-Hui Li Lu Zhang Dan Zhang Wei-Hua Cao Tian-Lin Qi Hong-Xiao Hao Xing-Yue Wang chong-Ping Ran Xiao-Jing Qu Shun-Ai Liu Yao Lu Ge Shen Shu-Ling Wu Min Chang Ru-Yu Liu Lei-Ping Hu Wen-Hao Hua Gang Wan Jun Cheng Yao Xie Liver Diseases Center Beijing Ditan Hospital Capital Medical University Beijing 100015 China Institute of Infectious Diseases Beijing DitanHospitai Capital Medical University Beijing 100015 China Clinical Test Center Beijing Ditan Hospital Capital Medical University Beijing 100015 China

Background： Plasmacytoid dendritic cells （pDCs） and cytokines play an important role in occurrence and recovery of hepatitis B virus （HBV） infection. The aim of this study was to explore the frequency and function ofpDC and serum cytokine network profiles in patients with acute or chronic HBV infection. Methods： The healthy individuals （HI group）, hepatitis B envelope antigen （HBeAg）-positive chronic HBV patients in immune tolerance （IT） phase （IT group）, HBeAg-positive chronic HBV patients （CHB group）, and acute HBV patients （AHB group） were enrolled in this study. The frequency of cluster of differentiation antigen 86 （CD86）＋ pDC and the counts of CD86 molecular expressed on surface ofpDC were tested by flow cytometer. The quantitative determinations ofcytokines, including Fins-like tyrosine kinase 3 ligand （FIt-3L）, interferon （1FN）-α2, IFN-γ, interleukin （il）-17A, il-6, il-10, transforming growth factor （TGF）-β1 and TGF-β2, were performed using Luminex multiplex technology. Results： In this study, there were 13 patients in HI group, 30 in IT group, 50 in CHB group, and 32 in AHB group. Compared with HI group, HBV infected group （including all patients in IT, CHB and AHB groups） had significantly higher counts of CD86 molecular expressed on the surface ofpDC （4596.5 ± 896.5 vs. 7097.7 ± 3124.6; P 〈 0.001）. The counts of CD86 molecular expressed on the surface of pDC in CriB group （7739.2 ±4125.4） was significantly higher than that of IT group （6393.4 ± 1653.6, P=0.043）. Compared with IT group, the profile of cytokines of FIt-3L, IFN-γ, and il-17A was decreased, IFN-α2 was significantly increased （P =0.012） in CH B group. The contents of il-10, TGF-{31, and TGF-132 in AHB group were significantly increased compared with IT and CHB groups （all P 〈 0.05）. Conclusions： This study demonstrated that the function of pDC was unaffected in HBV infection. The enhanced function of pDC and IFN-α2 might involve triggering the immune response from IT to hepatitis active phase

关键词： Acute Hepatitis B Virus Infection Chronic Hepatitis B Virus Infection Interferon-α2 Interleukin-10 PlasmacytoidDendritic Cells

推拿调控局部炎性微环境对腰椎间盘突出症大鼠疼痛行为的影响

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中华中医药杂志 2022年第9期37卷 5379-5384页

作者：姚重界孔令军朱清广程艳彬汤程吕智桢房敏上海中医药大学附属岳阳中西医结合医院上海200437 上海市中医药研究院推拿研究所上海200437 上海中医药大学针灸推拿学院上海201203

目的:观察局部炎性微环境与大鼠疼痛行为之间的相关性,探讨推拿干预腰椎间盘突出症(LDH)相关疼痛的可能机制。方法:将32只大鼠随机分为空白组、假手术组、模型组、推拿组,每组8只。推拿组在造模后第4天开始进行推拿干预。实验过程中行... 详细信息

目的:观察局部炎性微环境与大鼠疼痛行为之间的相关性,探讨推拿干预腰椎间盘突出症(LDH)相关疼痛的可能机制。方法:将32只大鼠随机分为空白组、假手术组、模型组、推拿组,每组8只。推拿组在造模后第4天开始进行推拿干预。实验过程中行大鼠右后足缩足阈值(PWT)测试、热缩足反射潜伏期(PWL)测试及Catwalk步态测试。实验结束后取大鼠背根神经节(DRG)组织,采用HE染色观察大鼠DRG组织病理形态变化;采用ELISA法检测组织中炎症相关因子肿瘤坏死因子(TNF)-α、白细胞介素(il)-1β、il-6的表达情况。结果:模型组大鼠造模后至第14天PWT和PWL持续降低,而从第14天至21天的变化趋于稳定,并显著低于同期空白组(Pil-1β、il-6的表达显著高于空白组(P<0.01),而推拿后可以显著降低这些因子表达(P<0.01)。结论:局部炎性微环境的改变可能会导致大鼠的疼痛行为,而推拿干预或可通过调控局部炎性微环境来缓解疼痛。

关键词：推拿腰椎间盘突出症炎症疼痛

KATP通道在参附注射液减轻大鼠心肌缺血再灌注损伤中的作用

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中华麻醉学杂志 2007年第3期27卷 213-216页

作者：牟崇明陈玉培重庆医科大学附属第二医院麻醉科 400010 现在大连医科大学附属第二医院麻醉科

目的研究KATP通道在参附注射液（SFI）减轻大鼠心肌缺血再灌注损伤中的作用。方法24只SD大鼠,雌雄不拘,体重240～320 g,随机分为4组（n=6）：对照组（C组）、缺血再灌注组（I/ R组）、SFI组和SFI＋KATP通道阻滞剂格列苯脲组（SG组）。C... 详细信息

目的研究KATP通道在参附注射液（SFI）减轻大鼠心肌缺血再灌注损伤中的作用。方法24只SD大鼠,雌雄不拘,体重240～320 g,随机分为4组（n=6）：对照组（C组）、缺血再灌注组（I/ R组）、SFI组和SFI＋KATP通道阻滞剂格列苯脲组（SG组）。C组仅穿线不结扎左冠状动脉前降支,穿线后静脉输注生理盐水8 ml/kg。采用结扎左冠状动脉前降支40 min再灌注120 min制备心肌缺血再灌注损伤模型。SFI组缺血前15 min静脉输注SFI 8 ml/kg,SG组给予格列苯脲0．33 mg/kg和SFI 8 ml/ kg。再灌注120 min后从心尖穿刺抽血,测定血清cTnI与il-6浓度,抽血后立即取心肌组织,测定心肌组织SOD活性、MDA含量、TNF-α表达,并在电镜下观察心肌超微结构。结果与C组比较,I/R组心肌SOD活性降低,心肌MDA、TNF-α和血清il-6、cTnI水平升高（P＜0．05）;与I/R组比较,SFI组、SG组心肌SOD活性升高,心肌MDA、TNF-α和血清cTnI、il-6水平降低（p＜0．05）;SG组与SFI组比较上述指标差异无统计学意义（P＞0．05）。SFI组与SG组心肌超微结构损伤程度明显轻于I/R组。结论参附注射液可以减轻大鼠心肌缺血再灌注损伤,其作用机制与KATP通道的激活无关。

关键词：钾通道参附汤心肌再灌注损伤

膜联蛋白A1的N末端肽Ac2-26对高糖刺激的小鼠巨噬细胞极化的影响

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中国病理生理杂志 2022年第11期38卷 1998-2004页

作者：夏崇建黄俊杰张煜卓嘉英吴娟娟陈刚范琰琰温州医科大学基础医学院浙江温州325035

目的:探讨膜联蛋白A1(ANXA1)的N末端肽Ac2-26对高糖刺激的小鼠巨噬细胞极化的影响及其机制。方法:体外培养小鼠骨髓来源的巨噬细胞(BMDM),将其分为对照组、高糖组、高糖+Ac2-26组、高糖+Ac2-26+甲酰肽受体2(FPR2)拮抗剂WRW4组和高糖+Ac2... 详细信息

目的:探讨膜联蛋白A1(ANXA1)的N末端肽Ac2-26对高糖刺激的小鼠巨噬细胞极化的影响及其机制。方法:体外培养小鼠骨髓来源的巨噬细胞(BMDM),将其分为对照组、高糖组、高糖+Ac2-26组、高糖+Ac2-26+甲酰肽受体2(FPR2)拮抗剂WRW4组和高糖+Ac2-26+磷脂酰肌醇3-激酶(PI3K)抑制剂wortmannin组。ELISA检测BMDM上清液中肿瘤坏死因子α(TNF-α)、白细胞介素6(il-6)、il-10和转化生长因子β1(TGF-β1)的分泌;Western blot检测BMDM中诱导型一氧化氮合酶(iNOS;M1型极化标志物)、CD206(M2型极化标志物)及ANXA1的表达,并检测PI3K和蛋白激酶B(PKB/AKT)的表达及磷酸化水平。结果:高糖刺激可以显著上调小鼠BMDM的iNOS表达及促炎因子(TNF-α和il-6)产生(Pil-10)产生(Pil-6)产生(Pil-10产生及PI3K/AKT的磷酸化水平(P<0.05);而WRW4和wortmannin能够显著抑制Ac2-26的效应(P<0.05)。结论:高糖刺激可以诱导小鼠BMDM向促炎的M1型极化,而Ac2-26可以抑制M1型极化,并促进BMDM向抗炎的M2型极化;Ac2-26的调节机制可能涉及FPR2/PI3K/AKT信号通路的激活。

关键词：糖尿病巨噬细胞极化膜联蛋白A1 Ac2-26 FPR2/PI3K/AKT信号通路

Effect of Lycopene on Inflammatory Response and TLR4 Pathway of Choroid Plexus in β-amyloid Induced Model of Alzheimer's Disease

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Effect of Lycopene on Inflammatory Response and TLR4 Pathway...

粉防己碱对急性出血坏死性胰腺炎大鼠炎性细胞因子含量的影响

中国营养学会第十次特殊营养学术会议

作者： LIU chong-bin JI Tao WANG Li-qun YI Yan-feng WANG Rui GAO Xi-ren Department of Physiology Basic Medical Science Building Huzhou Normal College

Objective: The present study was designed to investigate the neuroprotective potential of lycopene on inflammatory response and TLR4 pathway of choroid plexus inβ-amyloid induced model of Alzheimer’s disease. Methods: 40 adult SD rats(Body weight:250±18.7 g) were randomly divided into five groups(n= 8): control, AD model, AD+lycopene(ig.5 mg/kg.d), AD+lycopene(ig. 10 mg/kg.d) and lycopene group. The rats were adapted for one week prior to the present experiment. 8μgβ-amyloid(Aβ) was administered through intracerebroventricular(ICV) by using stereotaxic instrument in AD group and the AD+lycopene group rats’ bilateral hippocampus, and the control and lycopene groups with equal saline. All the rats were trained on a Morris water maze test after 2, 3, 4, 5 weeks to identify leaming and memory ability of the rats. The rats were sacrificed and the blood were prepared for the quantification of TNF-α, il-1β and il-6β level by ELISA. The expression of TLR4, NF-κB p65 m RNA and protein was detected by RT-q PCR and western blot. Western blot was also used to detect the expression of PS-1 and β-APP in hippocampus. Results: Compared with the control group, the latent period of escape and activity time in AD group was significantly longer(Pil-1β and il-6β in AD group was significantly incresed(P<0.05), but significantly decresed in AD+lycopene groups(P< 0.05). Compared with the control group, the expression of TLR4, NF-κB p65 mRNA and protein were also significantly incresed(P< 0.05), but significantly decresed in AD+lycopene groups(P< 0.05). Along with this,compared with the control group, the expression of PS-1 and β-APP in hippocampus were significantly incresed(P< 0.05), but were reversed on lycopene treatment(P< 0.05). Conclusion:Collectively, these observations provide an evidence for beneficial effect of lycopene supplementation in β-a

关键词： Lycopene Choroid Plexus Alzheimer’s Disease Inflammatory response

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中国药学杂志 2005年第13期40卷 983-985页

作者：秦大莲余崇林刘明华李华李亮泸州医学院药理教研室四川泸州646000 泸州医学院解剖学教研室四川泸州646000

目的通过测定血清及胰腺组织炎性细胞因子含量,研究粉防己碱治疗大鼠急性出血坏死性胰腺炎的机制。方法胆胰管逆行加压注射4.5%牛磺胆酸钠制备急性出血坏死性胰腺炎(AHNP)动物模型;ELISA法测炎性细胞因子白细胞介素1(il-1)、白细胞介素6... 详细信息

目的通过测定血清及胰腺组织炎性细胞因子含量,研究粉防己碱治疗大鼠急性出血坏死性胰腺炎的机制。方法胆胰管逆行加压注射4.5%牛磺胆酸钠制备急性出血坏死性胰腺炎(AHNP)动物模型;ELISA法测炎性细胞因子白细胞介素1(il-1)、白细胞介素6(il-6)、肿瘤坏死因子(TNF-α)含量。结果6h后AHNP大鼠血清及胰腺组织il-1,il-6,TNF-α明显升高。60及30mg·kg-1粉防己碱均可降低血清及胰腺组织内il-1,il-6及TNF-α的含量。结论粉防己碱对大鼠急性出血坏死性胰腺炎的治疗作用与降低血清及胰腺组织il-1,il-6,TNF-α含量有关。

关键词：粉防己碱急性出血坏死性胰腺炎大鼠炎性细胞因子