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Asian Journal of Andrology 2023年第6期25卷 708-712页

作者： Jun Zhao Xiao-Qiang Zhai He-Cheng Li tie chong Department of Urology The Second Affiliated HospitalXi’an Jiaotong UniversityXi’an 710016China

Vasectomy damage is a common complication of open nonmesh hernia *** study was a retrospective analysis of the characteristics and possible causes of vas deferens injuries in patients exhibiting unilateral or bilateral vasal obstruction caused by open nonmesh inguinal *** site of the obstructed vas deferens was intraoperatively ***,surgical methods,and patient outcomes were *** Anderson–Darling test was applied to test for Gaussian distribution of ***’s exact test or Mann–Whitney U test and unpaired t-test were used for statistical *** mean age at operation was 7.23(standard deviation[s.d.]:2.09)years and the mean obstructive interval was 17.72(s.d.:2.73)***(n=1)and inguinal(n=42)vasovasostomies were *** overall patency rate was 85.3%(29/34).Among the 43 enrolled patients(mean age:24.95[s.d.:2.20]years),73 sides of their inguinal regions were *** disconnected end of the vas deferens was found in the internal ring on 54 sides(74.0%),was found in the inguinal canal on 16 sides(21.9%),and was found in the pelvic cavity on 3 sides(4.1%).Location of the vas deferens injury did not significantly differ according to age at the time of hernia surgery(≥12 years or<12 years)or obstructive interval(≥15 years or<15 years).These results underscore that high ligation of the hernial sac warrants extra caution by surgeons during open nonmesh inguinal herniorrhaphy.

关键词： iatrogenic vas deferens injury obstructive azoospermia vasovasostomy

Curcumin induces the expression of NF-κB and Bcl-2/Bax in human renal cell carcinoma cell line ACHN

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Journal of Nanjing Medical University 2009年第6期23卷 386-391页

作者： Gang Li tie chong Ziming Wang Department of Urology the Second Affiliated Hospital of Xi an Jiaotong University Xi an 710004 China

Objective： To explore the in vitro effects of curcumin on the proliferation and apoptosis of the human renal cell carcinoma cell line ACHN, and to investigate its mechanisms of action. Methods： The human renal cell carcinoma cell line ACHN was treated with different concentrations of curcumin for 24 h. The MTT assay was used to evaluate the cytotoxic effects of curcumin and flow cytometry was utilized to observe and detect the apoptosis of ACHN cells induced by curcumin. The expression levels of Bcl-2, Bax and NF-κBP65 mRNA were evaluated by Reverse Transcription-Polymerase Chain Reaction （RT-PCR）, while the expression of Bcl- 2, Bax, NF-κBP65 and IκB proteins was evaluated by Western blot. Results： The concentrations of curcumin used significantly inhibited the proliferation of ACHN human renal cell carcinoma cells in vitro in a dose and time-dependent manner （Ftime=5.55, P 〈 0.05; Fdose=110.05, P 〈 0.05）. Obvious apoptosis of cells treated with different concentrations of curcumin could be observed by FCM. Compared with the control group, the apoptosis rates of curcumin-treated cells were markedly increased （F=96.35, P 〈 0.05）. Lower dose of curcumin significantly induced the apoptosis of ACHN cells. With intervention of different concentrations of curcumin （0, 10, 20 and 40 μmol/L） for 24 h, the expression levels of Bcl-2 and NF-κBP65 mRNA in ACHN cells were decreased while the expression level of Bax mRNA was increased （P 〈 0.05）, and Bcl-2, and NF-κBP65 protein decreased, while Bax and IκB protein increased compared with those in the untreated group. Conclusion： Curcumin inhibited proliferation and increased apoptosis of the human renal cell carcinoma cell line ACHN. These curcumin effects appear to involve up-regulating IκB, down-regulating NF-κB, and regulating the expression of the apoptosis genes Bcl-2/Bax.

关键词： curcumin renal cell carcinoma apoptosis NF-κB Bcl-2

Analysis of results related to the percent free prostate specific antigen among men without prostate diseases in Xi'an area

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Journal of Nanjing Medical University 2007年第1期21卷 25-28页

作者： Peng Zhang Ziming Wang tie chong Lihua Zhao Department of Urology the Second Affiliated Hospital of Medical College of Xi＇an JiaoTong University Xi＇ an 710004 China Department of clinical laboratory the Second Affiliated Hospital of Medical College of Xi＇ an JiaoTong University Xi＇ an 710004China

To measure the percent of free prostate specific antigen （fPSA） among men without prostate diseases in Xi＇an area, and to study the relationship of percent fPSA with age and pathological grade, clinical stage of prostate cancer （PCa） with percent fPSA, and to analyze the difference between the data in China and *** data to determine appropriate reference range for Chinese male. Methods： A total of 713 participants were enrolled into the study, with PSA, fPSA in serum measured and the percent fPSA calculated. Out of 713 cases, 679 without prostate diseases were divided into 5 groups by age, and then the relationships of PSA, fPSA and percent fPSA with age were studied, respectively. The relationship of pathological grade and clinical stage with percent fPSA of the 34 participants with PCa was also studied. With the help of the related data of men without prostate disease, the appropriate reference range for Chinese male was established. Results： The increases in PSA or fPSA were correlated with age, while there was no significant correlation between age and percent fPSA. The percent fPSA was also correlated with pathological grade and clinical stage of PCa. The percent fPSA of men without prostate disease in Xi＇an area was significandy lower than that in the related overseas data. The reference range of percent fPSA for Chinese male was≥ 15%. Conclusion： Percent fPSA might be more useful than PSA in the detection of prostate cancer. As the percent fPSA is decreased, the pathological grade is decreased, and the clinical stage is increased, the malignant degree is increased. The reference range of ≥15% is more appropriate for Chinese male.

关键词： percentage free prostate specific antigen prostate cancer Age relativity reference range Xi'an area

Isoflavones and Prostate Cancer： A Review of Some Critical Issues

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Chinese Medical Journal 2016年第3期129卷 341-347页

作者： Hong-Yi Zhang Jie Cui Ye Zhang Zhen-Long Wang tie chong Zi-Ming Wang Department of Urology Second Affiliated Hospital of Xi'an Jiaotong University Xi'an Shaanxi 710004 China Department of Urology Yan'an University Affiliated Hospital Yan'an Shaanxi 716000 China Department of Oncology Yan'an University Affiliated Hospital Yan'an Shaanxi 716000 China

Objective： The purpose of this review is to discuss some critical issues of isoflavones protective against the development of prostate cancer （PCa）.Data Sources： Data cited in this review were obtained primarily from PubMed and Embase from 1975 to *** Selection： Articles were selected with the search terms ＆quot;isoflavone＆quot;, ＆quot;Phytoestrogen＆quot;, ＆quot;soy＆quot;, ＆quot;genistin＆quot;, and ＆quot;PCa＆quot;.Results： Isoflavones do not play an important role on prostate-specific antigen levels reduction in PCa patients or healthy *** effect ofisoflavones on sex hormone levels and PCa risk may be determined by equol converting bacteria in the intestine, specific polymorphic variation and concentrations of *** intake of various types of phytoestrogens with lower concentrations in the daily diet may produce synergistic effects against ***, prostate tissue may concentrate isoflavones to potentially anti-carcinogenic *** addition, it is noteworthy that isoflavones may act as an agonist in ***： Isoflavones play a protective role against the development of ***, careful consideration should be given when isoflavones are used in the prevention and treatment of PCa.

关键词： Isoflavone Phytoestrogen Prostate Cancer

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米拉贝隆治疗膀胱过度活动症的临床应用

山东大学学报（医学版） 2018年第3期56卷 6-11页

作者：种铁陈琦西安交通大学第二附属医院(西北医院)泌尿外科陕西西安710004

米拉贝隆是一种强效的选择性β_3受体激动剂,是治疗膀胱过度活动症(OAB)有效且耐受性良好的新型药物。临床研究证实,米拉贝隆可明显减少尿失禁次数、排尿次数并增加排尿量,在改善尿急发生和夜尿增多等方面也有很好的疗效,联合抗胆碱能... 详细信息

米拉贝隆是一种强效的选择性β_3受体激动剂,是治疗膀胱过度活动症(OAB)有效且耐受性良好的新型药物。临床研究证实,米拉贝隆可明显减少尿失禁次数、排尿次数并增加排尿量,在改善尿急发生和夜尿增多等方面也有很好的疗效,联合抗胆碱能药物后可提高疗效,但不增加药物不良反应发生率,为OAB提供了新的治疗手段,具有良好的应用前景。

关键词：米拉贝隆膀胱过度活动症 β_3受体激动剂临床应用

Development and characterization of urethral stricture model in rabbits

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Journal of Medical Colleges of PLA(China) 2010年第6期25卷 351-358页

作者： Fu Delai Wang Ziming chong tie Li Hecheng Zhang Huibo Zhang Peng Gan Weimin Department of Urology Second Affiliated Hospital School of Medicine Xi 'an Jiaotong University Xi 'an 710004 China Department of Urology Affiliated Hospital of Shaanxi Traditional Chinese Medical College Xianyang 712000 China

Objective： To establish an experimental model of urethral stricture in rabbits. Methods： A total of 21 adult male New Zealand rabbits were included into group. After intravenous anesthesia, urethroscopy was performed with a pediatric resectoscope （F13）. Fifteen animals were randomly selected as the study group. A lcm-long circumferential electrocoagulation of the bulbar urethra was performed to these animals until ulceration of the mucosa. The remaining 6 animals underwent video urethroscopy without electrocoagulation, serving as controls. On the 30th day, retrograde urethrogram and urethroscopy were performed to evaluate urethral stricture formation, histological examinations （HE and Sirius Red staining） were done to assess urethral pathological change. Results： Two rabbits in study group died and no death occurred in controls. Based on urethrogram and urethroscopy, no rabbits in control group developed urethral stricture, while significant stricture formation was observed in every case of the study group. Histological examination showed a normal urethra in control cases, while at stricture site of the study group extensive fibrosis of muscle and submucous tis High collagen expression in fibrosis tissue was assayed sue by was observed with a large number of fibroblasts infiltration Sirius Red staining. Conclusion： A lcm-long endoscopic electrocoagulation can successfully induce urethral stricture formation in rabbit models. This method offers an ideal animal model for the fundamental and clinical study of urethral stricture

关键词： Urethral stricture Animal model Rabbits

姜黄素对裸鼠人ACHN肾癌移植瘤的放射增敏作用

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西安交通大学学报（医学版） 2019年第2期40卷 284-289页

作者：李刚刘莹种铁王子明西安交通大学第二附属医院泌尿外科陕西西安710004

目的探讨姜黄素对裸鼠人ACHN肾癌移植瘤的放射增敏作用及其机制。方法建立人ACHN肾癌裸鼠皮下移植瘤模型;筛选合适的姜黄素浓度和放射性剂量进行实验;随机分组进行药物或放射线干预,观察不同处理组裸鼠移植瘤生长情况,确定姜黄素对裸鼠... 详细信息

目的探讨姜黄素对裸鼠人ACHN肾癌移植瘤的放射增敏作用及其机制。方法建立人ACHN肾癌裸鼠皮下移植瘤模型;筛选合适的姜黄素浓度和放射性剂量进行实验;随机分组进行药物或放射线干预,观察不同处理组裸鼠移植瘤生长情况,确定姜黄素对裸鼠移植瘤的放射增敏作用;采用流式细胞仪检测不同组移植瘤细胞凋亡;Western blot检测不同组DNA损伤修复相关蛋白DNA-PKcs和NF-κB信号通路相关蛋白的表达。结果细胞悬液接种法可成功建立人ACHN肾癌移植瘤模型,成瘤率85.7%;采用总剂量10 Gy放射剂量和25 mg/kg姜黄素进行实验;移植瘤生长曲线显示姜黄素组、放射组和实验组的抑瘤率分别为7.93%、49.20%、71.42%,实验组对移植瘤生长抑制效果明显高于姜黄素组及放射组(P1.4;与放射组相比,实验组的凋亡率显著升高(P<0.05),DNA-PKcs和NF-κB P65蛋白的表达水平显著下降(P<0.05),I-κB蛋白的表达显著升高(P<0.05),NF-κB下游调控蛋白VEGF、COX_2和Bcl-2蛋白的表达水平亦显著降低(P<0.05)。结论姜黄素对人ACHN肾癌裸鼠移植瘤具有放射增敏作用;姜黄素的放射增敏作用与其诱发肿瘤细胞凋亡、抑制DNA损伤修复和抑制NF-κB信号通路的激活密切相关。

关键词：姜黄素肾细胞癌凋亡放射增敏 NF-κB

eIF4E在肾透明细胞癌组织中的表达及意义

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西安交通大学学报（医学版） 2019年第1期40卷 106-110,125页

作者：李刚刘莹种铁王子明西安交通大学第二附属医院泌尿外科陕西西安710004

目的探讨真核细胞翻译起始因子4E(eukaryotic translation initiation factor 4E,eIF4E)在人肾癌组织中的表达及意义。方法采用免疫组织化学法检测40例肾癌组织及40例癌旁组织中eIF4E蛋白的表达。RT-PCR检测eIF4EmRNA在不同临床分期的... 详细信息

目的探讨真核细胞翻译起始因子4E(eukaryotic translation initiation factor 4E,eIF4E)在人肾癌组织中的表达及意义。方法采用免疫组织化学法检测40例肾癌组织及40例癌旁组织中eIF4E蛋白的表达。RT-PCR检测eIF4EmRNA在不同临床分期的肾癌组织与癌旁组织中的表达。Western blot法检测不同临床分期的肾癌组织与癌旁组织中eIF4E和Phospho-eIF4E蛋白的表达。结果 eIF4E蛋白在肾癌组织阳性表达率为77.5%,癌旁组织阳性表达率为27.5%,肾癌组织中eIF4E阳性表达率明显高于癌旁组织(P0.05)。结论eIF4E和PhosphoeIF4E在肾透明细胞癌中高表达,与肾癌的发生、发展有关,为肾癌的诊断及治疗提供了理论依据。

关键词：真核细胞翻译起始因子4E(eIF4E) Phospho-eIF4E 肾细胞癌细胞分化

提高认识,不断优化辅导员队伍建设的运行机制

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福建教育学院学报 2007年第10期8卷 9-11页

作者：赵崇铁福建教育学院学生工作处福建福州350025

要从党的事业高度,高校发展与稳定的角度和辅导员队伍人才辈出的历史维度来认识辅导员队伍建设的重要性。不断优化辅导员的选聘、构成、管理、工作、职责掌控、培养和职业发展等运行机制,进一步促进辅导员队伍建设。

关键词：辅导员队伍建设认识机制

深化新时代基础教育评价改革——学习贯彻《深化新时代教育评价改革总体方案》精神

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福建教育学院学报 2020年第11期21卷 1-2,50,129页

作者：赵崇铁福建教育学院基础教育研究院福建福州350025

中共中央、国务院发布的《深化新时代教育评价改革总体方案》为新时代我国教育评价改革提供了任务书和施工图,是一份事关教育发展方向的重要文件。文件“总体要求”明确提出了要坚持立德树人、问题导向、科学有效、统筹兼顾、中国特色... 详细信息

中共中央、国务院发布的《深化新时代教育评价改革总体方案》为新时代我国教育评价改革提供了任务书和施工图,是一份事关教育发展方向的重要文件。文件“总体要求”明确提出了要坚持立德树人、问题导向、科学有效、统筹兼顾、中国特色等原则,这“五个坚持”是深化新时代教育评价改革的基本遵循,我们要认真学习领会并在办学中实现实施全面而深入的改革。

关键词：教育评价改革立德树人教育发展