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Milk protein concentrate and reduced-calcium milk protein concentrate as natural emulsifiers in clean label high-protein ice cream manufacture

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Journal of Future Foods 2023年第2期3卷 175-182页

作者： Joseph Paglia chloe fung Chi Kong Yeung Animal Science Department California Polytechnic State UniversitySan Luis Obispo 93407USA Food Science and Nutrition Department California Polytechnic State UniversitySan Luis Obispo 93407USA

Clean label food is a rising consumer trend in the food *** protein concentrate(MPC)and reduced-calcium milk protein concentrate(RCMPC)could serve as natural emulsifiers and increase the total protein content of ice cream *** objectives of this study were to determine and compare the effects of MPC and RCMPC on ice cream composition,mix viscosity,storage stability,meltdown rate,and texture.A base formulation with 3%non-fat dry milk(NFDM)and no added emulsifiers was set as the *** levels of MPC or RCMPC(each powder containing 85%protein)at 1%,2%,and 3%were incorporated by replacing equivalent amounts of NFDM and keeping other ingredients *** ice cream treatments were processed with a target overrun of 70%and hardened at−25℃in a blast *** of MPC or RCMPC at 1%,2%,and 3%corresponded to increases in protein content of ice cream by 15%,30%,and 45%,*** viscosity of the ice-cream mix increased with increasing levels of MPC or *** general,higher protein samples had slower meltdown rate and higher values of hardness and adhesiveness,but the trends were *** shrinkage in volume was observed in any ice cream stored at−25℃after 180 ***,an additional storage stability study revealed that the control showed significant shrinkage after 60 days(−6.5%±1.5%),90 days(−7.1%±1.8%),and 180 days(−7.9%±1.1%)in a typical household-style freezer at−13℃.MPC at 1%also showed significant shrinkage after 180 days,while samples with RCMPC at any levels showed no shrinkage at *** cream manufacturers may consider MPC and RCMPC natural alternatives to synthetic emulsifiers,with RCMPC being more effective than MPC in terms of ice cream storage stability.

关键词： Ice cream Clean label High-protein Milk protein concentrate Reduced-calcium milk protein concentrate

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Molecular basis of von Hippel-Lindau syndrome in Chinese patients

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Chinese Medical Journal 2011年第2期124卷 237-241页

作者： SIU Wai-kwan MA Ronald Ching-wan LAM Ching-wan MAK chloe Miu YUEN Yuet-ping LO Fai-man Ivan CHAN Kin-wan LAM Siu-fung LING Siu-cheung TONG Sui-fan SO Wing-yee CHOW Chun-chung TANG Mary Hoi-yin TAM wing-hung CHAN Albert Yan-wo Department of Pathology Princess Margaret Hospital Hong Kong China Department of Medicine & Therapeutics The Chinese University of Hong Kong Prince of Wales Hospital Hong Kong China Department of Pathology Li Ka Shing Faculty of Medicine The University of Hong Kong Queen Mary Hospital Hong Kong China Clinical Genetic Service Department of Health Hong Kong China Department of Medicine and Geriatrics Princess Margaret Hospital Hong Kong China Department of Paediatrics and Adolescent Medicine Princess Margaret Hospital Hong Kong China Department of Obstetrics & Gynaecology Tsan Yuk Hospital Hong Kong China Department of Obstetrics & Gynaecology The Chinese University of Hong Kong Prince of Wales Hospital Hong Kong China

Background Von Hippel-Lindau （VHL） syndrome is an autosomal dominant familial cancer syndrome predisposing the affected individuals to multiple tumours in various *** genetic basis of VHL in Southern Chinese is largely *** this study,we characterized the mutation spectrum of VHL in nine unrelated Southern Chinese *** Nine probands with clinical features of VHL,two symptomatic and eight asymptomatic family members were included in this *** diagnosis was performed twice for one *** probands had only isolated bilateral *** VHL gene was screened for mutations by polymerase chain reaction,direct sequencing and multiplex ligation-dependent probe amplification （MLPA）.Results The nine probands and the two symptomatic family members carried heterozygous germline *** different VHL mutations were identified in the nine *** splicing mutation,NM_000551.2：c.463＋1G〉T,was *** other seven VHL mutations,c.233A〉G [***78Ser],c.239G〉T [***80lle],c.319C〉G [***107Gly],c.481C〉T [***161X],c.482G〉A [***161GIn],c.499C〉T [***167Trp] and an exon 2 deletion,had been previously *** asymptomatic family members were positive for the mutation and the other five tested *** prenatal diagnosis,the fetuses were positive for the *** Genetic analysis could accurately confirm VHL syndrome in patients with isolated tumours such as sporadic phaeochromocytoma or epididymal papillary *** detection in asymptomatic family members allows regular tumour surveillance and early intervention to improve their ***-based diagnosis can have an important impact on clinical management for VHL families.

关键词： von Hippel-Lindau syndrome VHL gene Chinese VHL mutations

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