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Disruption of ectoplasmic specializations between Sertoli cells and maturing spermatids by anti-nectin-2 and anti-nectin-3 antibodies

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Asian Journal of Andrology 2008年第4期10卷 577-584页

作者： Yoshiro Toyama Fumie Suzuki-Toyota Mamiko Maekawa chizuru ito Kiyotaka Toshimori Department of Anatomy and Developmental Biology Graduate School of Medicine Chiba University Chiba 260-8670Japan

Aim： To understand the biological functions of the ectoplasmic specializations between Sertoli cells and maturing spermatids in seminiferous epithelia. Methods： In order to disrupt the function of the ectoplasmic specializations, nectin-2, which is expressed at the specialization, was neutralized with anti-nectin-2 antibody micro-injected into the lumen of the mouse seminiferous tubule. Anti-nectin-3 antibody was also micro-injected into the lumen in order to neutralize nectin-3, which is expressed at the specialization. Results： The actin filaments at the specialization disappeared, and exfoliation of maturing spermatids was observed by electron microscopy. Conclusion： Nectin-2 was neutralized by anti-nectin-2 antibody and nectin-3 was neutralized by anti-nectin-3 antibody, respectively. Inactivated nectin-2 and nectin-3 disrupted the nectin-afadin-actin system, and finally the actin filaments disappeared. As a result, the specialization lost the holding function and detachment of spermatids was observed. One of the functions of the specialization seems to be to hold maturing spermatids until spermiation.

关键词： ectoplasmic specialization Sertoli cell spermatogenic cell testis actin nectin mice

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Interaction between basigin and monocarboxylate transporter 2 in the mouse testes and spermatozoa

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Asian Journal of Andrology 2016年第4期18卷 600-606页

作者： Cheng Chen Mamiko Maekawa Kenji Yamatoya Masami Nozaki chizuru ito Toshihiko Iwanaga Kiyotaka Toshimori Department of Anatomy and Developmental Biology Graduate School of Medicine Chiba University Chiba Japan Department of Cell Biology Research Institute for Microbia Diseases Osaka University Osaka Japan Laboratory of Histology and Cytology Department of Anatomy Graduate School of Medicine Hokkaido University Hokkaido Japan

Basigin is a member of the immunoglobulin superfamily and plays various important roles in biological events including spermatogenesis. To examine the basigin molecular variants during spermatogenesis and sperm maturation in the mouse, immunoprecipitated basigin samples from testis and epididymal spermatozoa were analyzed by liquid chromatography-mass spectrometry/mass spectrometry （LC-MS/MS）. The results demonstrated that basigin molecules from the testis and spermatozoa were separable into two major bands and that the differences in the molecular sizes were possibly because of an endoproteolytic cleavage. Since basigin is known to be a chaperone for the monocarboxylate transporter 1 （MCT1）, the localization of basigin, MCT1 and MCT2 was examined during postnatal testicular development. Immunohistochemical studies showed different expression patterns of MCT1 and MCT2. MCT1 was localized on the surface of spermatogonia, spermatocytes, and spermatids. In contrast, MCT2 appeared on the principal piece of spermatozoa in the testis, where basigin was also observed. In mature epididymal spermatozoa, MCT2 was located on the midpiece, where basigin co-localized with MCT2 but not with MCT1. Furthermore, MCT2 was immunoprecipitated with basigin in mouse testes and sperm. These results suggest that basigin has a functional role as a binding partner with MCT2 in testicular and epididymal spermatozoa.

关键词： basigin monocarboxylate transporter sperm maturation spermatogenesis spermatozoa testis

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