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FERONIA is involved in phototropin 1-mediated blue light phototropic growth in Arabidopsis

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Journal of Integrative Plant Biology 2022年第10期64卷 1901-1915页

作者： chiyu li Jia Chen Xiaoyan li Xin Zhang Ying liu Sirui Zhu Long Wang Heping Zheng Sheng Luan Jiada li Feng Yu School of life Sciences Central South UniversityChangsha 410078China State Key Laboratory of Chemo/Biosensing and Chemometrics College of BiologyHunan UniversityChangsha 410082China Hunan Key Laboratory of Plant Functional Genomics and Developmental Regulation Hunan UniversityChangsha 410082China Department of Plant and Microbial Biology University of CaliforniaBerkeleyCA94720USA State Key Laboratory of Hybrid Rice Hunan Hybrid Rice Research CenterChangsha 410125China

Plant shoot phototropism is triggered by the formation of a light-driven auxin gradient leading to bending growth.The blue light receptor phototropin 1(phot1)senses light direction,but how this leads to auxin gradient formation and growth regulation remains poorly understood.Previous studies have suggested phot1’s role for regulated apoplastic acidification,but its relation to phototropin and hypocotyl phototropism is unclear.Herein,we show that blue light can cause phot1 to interact with and phosphorylate FERONIA(FER),a known cell growth regulator,and trigger downstream phototropic bending growth in Arabidopsis hypocotyls.fer mutants showed defects in phototropic growth,similar to phot1/2 mutant.FER also interacts with and phosphorylates phytochrome kinase substrates,the phot1 downstream substrates.The phot1-FER pathway acts upstream of apoplastic acidification and the auxin gradient formation in hypocotyl under lateral blue light,both of which are critical for phototropic bending growth in hypocotyls.Our study highlights a pivotal role of FER in the phot1-mediated phototropic cell growth regulation in plants.

关键词： blue light FERONIA H+and auxin gradience formation hypocotyl phototropin 1 phototropism

The RALF1-FERONIA Complex Phosphorylates eIF4E1 to Promote Protein Synthesis and Polar Root Hair Growth

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Molecular Plant 2020年第5期13卷 698-716页

作者： Sirui Zhu Jose Manuel Estevez Hongdong liao Yonghua Zhu Tao Yang chiyu li Yichuan Wang Lan li Xuanming liu Javier Martinez Pacheco Hongwei Guo Feng Yu State Key Laboratory of Chemo/Biosensing and Chemometrics College of Biologyand Hunan Key Laboratory of Plant Functional Genomics and Developmental RegulationHunan UniversityChangsha 410082P.R.China Fundacion Instituto Leloir Buenos Aires C1405BWEArgentina and IIBBA-CONICETBuenos Aires C1405BWEArgentina Centro de Biotecnologfa Vegetal Facultad de Ciencias de la VidaUniversidad Andres BelloSantiago RM 8370146Chile National Engineering Laboratory for Rice and Byproduct Deep Processing Central South University of Forestry and TechnologyChangsha 410004P.R.China Institute of Plant and Food Science Department of BiologySouthern University of Science and TechnologyShenzhenGuangdong 518055P.R.China State Key Laboratory of Hybrid Rice Hunan Hybrid Rice Research CenterChangsha 410125P.R.China

The molecular links between extracellular signals and the regulation of localized protein synthesis in plant cells are poorly understood.Here,we show that in Arabidopsis thaliana,the extracellular peptide RALF1 and its receptor,the FERONIA receptor kinase,promote root hair(RH)tip growth by modulating protein synthesis.We found that RALF1 promotes FERONIA-mediated phosphorylation of elF4E1,a eukaryotic translation initiation factor that plays a crucial role in the control of mRNA translation rate.Phosphorylated elF4E1 increases mRNA affinity and modulates mRNA translation and,thus,protein synthesis.The mRNAs targeted by the RALF1-FERONIA-elF4E1 module include ROP2 and RSL4,which are important regulators of RH cell polarity and growth.RALF1 and FERONIA are expressed in a polar manner in RHs,which facilitate elF4E1 polar丨ocalization and thus may control local f?OP2 translation.Moreover,we demonstrated that high-level accumulation of RSL4 exerts negative-feedback regulation of RALF1 expression by directly binding the RALF1 gene promoter,determining the final RH size.Our study reveals that the link between RALF1-FERONIA signaling and protein synthesis constitutes a novel component regulating cell expansion in these polar growing cells.

关键词： elF4E1 FER polar cell growth RALF1 root hairs RSL4

负载ZIF-8的GelMA水凝胶制备及其药物缓释性能和抑菌作用评价

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吉林大学学报（医学版） 2024年第1期50卷 106-112页

作者：姜孔昭李驰宇罗云纲刘志辉吉林大学口腔医院修复科吉林长春130021 吉林大学第二医院骨科吉林长春130022 吉林大学第一医院净月分院口腔科吉林长春130117

目的:制备一种含沸石咪唑类骨架材料8(ZIF-8)的复合光交联水凝胶,评价其体外细胞毒性、药物释放能力和抗菌性能。方法:采用水热法合成ZIF-8颗粒,扫描电子显微镜(SEM)观察ZIF-8颗粒微观形态表现。将ZIF-8颗粒以质量分数0.2%的比例与甲基... 详细信息

目的:制备一种含沸石咪唑类骨架材料8(ZIF-8)的复合光交联水凝胶,评价其体外细胞毒性、药物释放能力和抗菌性能。方法:采用水热法合成ZIF-8颗粒,扫描电子显微镜(SEM)观察ZIF-8颗粒微观形态表现。将ZIF-8颗粒以质量分数0.2%的比例与甲基丙烯酸酐化明胶(GelMA)混合获得复合水凝胶GelMA-Z。采用原子吸收光谱法检测各时间点GelMA-Z中锌离子(Zn^(2+))累计释放量。NIH-3T3细胞分为对照组、GelMA组和GelMA-Z组,将GelMA和GelMA-Z与NIH-3T3细胞共培养1、3和7 d,采用CCK-8法检测各组细胞存活率。大肠杆菌(E.coli)和金黄色葡萄球菌(S.aureus)分为对照组、GelMA组和GelMA-Z组,将GelMA和GelMA-Z分别与E.coli和S.aureus共培养6、12和24 h,采用酶标仪检测不同时间点各组细菌活性,采用平板抑菌实验和活/死细菌染色实验检测各组细菌菌落形成情况和活/死细菌染色情况。结果:SEM观察,采用水热法合成的ZIF-8颗粒具有均匀的粒径。原子吸收光谱法检测,GelMA-Z中Zn^(2+)在1 d内呈突释阶段后缓慢释放,7 d左右达到平衡。与对照组比较,1、3和7 d时GelMA组和GelMA-Z组细胞存活率均大于90%,差异无统计学意义(P>0.05)。酶标仪检测菌液活性,与细菌共培养6、12和24 h时,与对照组和GelMA组比较,GelMA-Z组E.coli和S.aureus活性明显降低(P<0.05)。平板抑菌实验,GelMA-Z组中菌液涂布后形成的菌落数明显少于对照组和GelMA组。活/死细菌染色实验,GelMA-Z组存在大量红色荧光染色的死细菌,对照组和GelMA组中则为大量绿色荧光染色的活细菌。结论:负载ZIF-8的GelMA水凝胶可实现原位光交联,具有良好的Zn^(2+)释放能力和抗菌性,是一种可用于感染伤口治疗的新型水凝胶敷料。

关键词：含沸石咪唑类骨架材料8 甲基丙烯酸酐化明胶水凝胶感染伤口抗菌性能

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山地旅游轨道交通单双线组合模式设计与评价

综合运输 2024年第3期46卷 67-71,144页

作者：张莉石义博李驰宇齐援盟刘澜杭州市地铁集团有限责任公司杭州310020 中铁二院华东勘察设计有限责任公司杭州310000 西南交通大学交通运输与物流学院成都610031 西南交通大学综合交通运输智能化国家地方联合工程实验室成都610031

为适应山地旅游轨道交通客流空间分布不均衡的特征,本文提出一种新的轨道交通运营模式——单双线组合运营。在详细分析山地旅游轨道交通客流空间特征、单双线确定原则的基础上,提出三种组合运营模式:单线+双线、单线+双线+单线、双线+单... 详细信息

为适应山地旅游轨道交通客流空间分布不均衡的特征,本文提出一种新的轨道交通运营模式——单双线组合运营。在详细分析山地旅游轨道交通客流空间特征、单双线确定原则的基础上,提出三种组合运营模式:单线+双线、单线+双线+单线、双线+单线+双线,并采用AHP-模糊综合评价法从客流运输需求、交通运输条件、工程建设、运营经济四个方面评价运营模式的可行性。以遂昌山地旅游轨道交通为例进行单双线组合模式的应用,算例表明:山地旅游轨道交通单双线组合运营模式下交通运输条件和运营经济均满足要求,线路的通过能力和客流需求有较好的适配性,相比全线双线工程建设投资可减少10.8%。

关键词：山地旅游轨道交通单双线组合运营客流特征 AHP-模糊综合评价

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物理微环境调控干细胞定向分化

生物医学工程学杂志 2023年第4期40卷 609-616页

作者：李驰宇樊瑜波郑丽沙北京航空航天大学生物与医学工程学院、北京市生物医学工程高精尖创新中心、生物力学与力生物学教育部重点实验室北京100083

干细胞具有自我更新和多向分化的能力,在组织工程和再生医学领域被认为有广泛的应用前景。然而,干细胞的功能依赖于自身所处的微环境即干细胞龛。干细胞龛中包含复杂的生物物理和生物化学因素。最近的研究表明,生物物理因素即干细胞所... 详细信息

干细胞具有自我更新和多向分化的能力,在组织工程和再生医学领域被认为有广泛的应用前景。然而,干细胞的功能依赖于自身所处的微环境即干细胞龛。干细胞龛中包含复杂的生物物理和生物化学因素。最近的研究表明,生物物理因素即干细胞所处的物理微环境在调控干细胞定向分化中发挥着重要的作用。在体外,人工合成的物理微环境能够精确操控多种生物物理特性。在此基础上,研究者进一步开展了如基质硬度、基质形貌和机械力等生物物理特性对各种不同种类的干细胞定向分化功能影响的研究。本文总结了人工构建物理微环境的手段及力学模型,并且综述了不同生物物理特性对干细胞分化影响的研究现状,希望对今后相关领域的研究发展提供参考借鉴。

关键词：干细胞分化微环境生物物理信号

Evaluation of antibody responses in healthy individuals receiving SARS-CoV-2 inactivated vaccines

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生物安全与健康(英文) 2024年第3期6卷 153-164页

作者： liu Ziyu Cai liyan Xing Man Qiao Nan liu Jiaojiao li Xuejun Zhang chiyu Tang Naijun Xu Zhelong Guo Yingying Lu Renfei Zhou Dongming Department of Pathogen Biology School of Basic Medical Sciences Tianjin Medical University Tianjin China Physical examination center Affiliated Nantong Hospital of Nantong University Nantong China Shanghai Public Health Clinical Center Fudan University Shanghai China Department of Occupational and Environmental Health School of Public Health Tianjin Medical University Tianjin China Clinical Laboratory Affiliated Nantong Hospital of Nantong University Nantong China

Inactivated coronavirus disease 2019 (COVID-19) vaccines such as CoronaVac and BBIBP-CorV have been widely used in China. However, more investigation is still needed to understand antibodies'' duration and effectiveness against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants in the real world. In this study, 575 participants who had been vaccinated with two or three doses of the inactivated vaccine were recruited. Serum samples were collected and tested for anti-spike IgG and neutralizing antibodies against SARS-CoV-2 (original strain, Dela, and Omicron). Unsurprisingly, a third dose of the vaccine significantly enhanced antibody responses against SARS-CoV-2 and its variants. However, despite a booster dose, the neutralizing antibody levels against Omicron, particularly the BA.5.2 subvariant, remained low. There was no sex bias, but an age bias was observed. Notably, the predominant IgG subclass antibodies were IgG1 and IgG2, with a much lower level of IgG4. After the booster shot, the ratio of IgG4 to IgG1 significantly increased. The observation of IgG1 to the IgG4 class switch after repeated inactivated vaccinations underscores the importance of continuous monitoring of subclass antibody responses. Further clinical investigations are required to understand the implications of this class switch for optimizing immunization strategies.

关键词： Variant of concern (VOC) Inactivated vaccines Serological study IgG subclasses Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)

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差异化市场下储能运营风险效益评估

太阳能学报 2022年第3期43卷 111-118页

作者：周立立向月延肖何李驰宇苟竞四川大学电气工程学院成都610065 新能源电力系统国家重点实验室(华北电力大学) 北京102206 国电大渡河瀑布沟水力发电总厂汉源625304 国网四川省电力公司经济技术研究院成都610041

储能得益于其充放电的灵活性可应用于多个电力市场并从中获取相应的效益。然而,储能在获利的同时也要承受来自不同市场的风险,且具有高利润的市场往往伴随着高风险,因此很有必要评估不同市场的风险效益并优化储能应用于不同市场的容量,... 详细信息

储能得益于其充放电的灵活性可应用于多个电力市场并从中获取相应的效益。然而,储能在获利的同时也要承受来自不同市场的风险,且具有高利润的市场往往伴随着高风险,因此很有必要评估不同市场的风险效益并优化储能应用于不同市场的容量,以期在最小的风险水平下实现效益最大化。提出一种差异化市场下的储能运营风险效益评估方法,考虑能源服务市场、对等交易服务市场、阻塞服务市场等3个储能可参与的市场,并应用投资组合理论建立各个市场下的收益率及风险计算模型及储能容量优化分配模型。研究结果表明提出的模型对于指导储能运营商参与不同市场的风险效益评估及其容量分配有较好的理论及现实指导意义。

关键词：储能电力市场风险分析效益评估容量优化分配

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党的文化领导力与文化自信自强的内在关系

中国领导科学 2023年第2期 46-53页

作者：李驰宇浙江工商大学马克思主义学院浙江省习近平新时代中国特色社会主义思想研究中心浙江工商大学研究基地

党的文化领导力与文化自信自强具有内生耦合性,两者融合为铸就社会主义文化新辉煌提供着源源不断的理论支持。党的文化领导力对文化自信自强具有政治导向定航、价值信念引领和文化软实力塑造的作用,推进文化自信自强又为文化领导力的提... 详细信息

党的文化领导力与文化自信自强具有内生耦合性,两者融合为铸就社会主义文化新辉煌提供着源源不断的理论支持。党的文化领导力对文化自信自强具有政治导向定航、价值信念引领和文化软实力塑造的作用,推进文化自信自强又为文化领导力的提升筑牢思想根基。因此,充分认识党的文化领导力与文化自信自强的本质内涵和辩证关系,考察两者关系关涉的核心议题,进而从拓展理论广度、提升知行深度、整合思维精度等方面来强化两者的契合程度,把文化自觉融入领导行为转化为文化领导力,将从整体上提升党的文化领导力水平,推进文化自信自强。

关键词：意识形态党的文化领导力文化自信自强

FGF21-mediated autophagy:Remodeling the homeostasis in response to stress in liver diseases

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Genes & Diseases 2024年第3期11卷 264-274页

作者： Wei Shen Modan Yang Hao Chen Chiy He Huigang li Xinyu Yang Jianyong Zhuo Zuyuan lin Zhihang Hu Di Lu Xiao Xu Zhejiang University School of Medicine HangzhouZhejiang 310058China Key Laboratory of Integrated Oncology and Intelligent Medicine of Zhejiang Province Department of Hepatobiliary and Pancreatic SurgeryAffiliated Hangzhou First People's HospitalZhejiang University School of MedicineHangzhouZhejiang 310006China The Institute for Organ Repair and Regenerative Medicine of Hangzhou HangzhouZhejiang 310006China Institute of Organ Transplantation Zhejiang UniversityHangzhouZhejiang 310003China National Center for Healthcare Quality Management in liver Transplant HangzhouZhejiang 310003China

liver diseases are worldwide problems closely associated with various stresses,such as endoplasmic reticulum stress.The exact interplay between stress and liver diseases remains unclear.Autophagy plays an essential role in maintaining homeostasis,and recent studies indicate tight crosstalk between stress and autophagy in liver diseases.Once the bal-ance between damage and autophagy is broken,autophagy can no longer resist injury or main-tain homeostasis.In recent years,FGF21(fibroblast growth factor 21)-induced autophagy has attracted much attention.FGF21 is regarded as a stress hormone and can be up-regulated by an abundance of signaling pathways in response to stress.Also,increased FGF21 activates autophagy by a complicated signaling network in which mTOR plays a pivotal role.This review summarizes the mechanism of FGF21-mediated autophagy and its derived application in the defense of stress in liver diseases and offers a glimpse into its promising prospect in future clinical practice.

关键词： Autophagy Fibroblast growth factor 21 liver diseases mTOR Stress