检索结果-南通市图书馆

Fructose promotes pyoluteorin biosynthesis via the CbrAB-CrcZ-Hfq/Crc pathway in the biocontrol strain Pseudomonas PA1201

引用

Synthetic and Systems Biotechnology 2023年第4期8卷 618-628页

作者： Ying Cui Kai Song Zi-Jing Jin Learn-Han Lee chitti thawai Ya-Wen He State Key Laboratory of Microbial Metabolism Joint International Research Laboratory of Metabolic and Developmental SciencesSchool of Life Sciences and BiotechnologyShanghai Jiao Tong UniversityShanghai200240China Novel Bacteria and Drug Discovery Research Group(NBDD) Microbiome and Bioresource Research Strength(MBRS)Jeffrey Cheah School of Medicine and Health SciencesMonash University MalaysiaBandar SunwaySelangor Darul Ehsan47500Malaysia Department of Biology Faculty of ScienceKing Mongkut’s Institute of Technology LadkrabangBangkok10520Thailand

Biocontrol strain Pseudomonas PA1201 produces pyoluteorin(Plt),which is an antimicrobial secondary *** represents a promising candidate pesticide due to its broad-spectrum antifungal and antibacterial *** PA1201 contains a complete genetic cluster for Plt biosynthesis,it fails to produce detectable level of Plt when grown in media typically used for Pseudomonas *** this study,minimum medium(MM)was found to favor Plt *** the medium M,which contains all the salts of MM medium except for mannitol,as a basal medium,we compared 10 carbon sources for their ability to promote Plt ***,mannitol,and glycerol promoted Plt biosynthesis,with fructose being the most effective carbon *** or succinic acid had no significant effect on Plt biosynthesis,but effectively antagonized fructose-dependent synthesis of ***-lacZ fusion reporter strains demonstrated that fructose acted through activation of the pltLABCDEFG(pltL)operon but had no effect on other genes of plt gene cluster;glucose or succinic acid antagonized fructose-dependent pltL ***,fructose-mediated Plt synthesis involved carbon catabolism *** two-component system CbrA/CbrB and small RNA catabolite repression control Z(crcZ)were essential for fructose-induced Plt *** small RNA binding protein Hfq and Crc negatively regulated fructose-induced *** together,this study provides a new model of fructose-dependent Plt production in PA1201 that can help improve Plt yield by biosynthetic approaches.

关键词： Pseudomonas Biocontrol Pyoluteorin Fructose Carbon catabolism repression

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

H-NS family protein MvaU downregulates phenazine-1-carboxylic acid(PCA)biosynthesis via binding to an AT-rich region within the promoter of the phz2 gene cluster in the rhizobacterium Pseudomonas strain PA1201

引用

Synthetic and Systems Biotechnology 2021年第4期6卷 262-271页

作者： Yun-Ling Fang Ying Cui Lian Zhou chitti thawai Tatheer Alam Naqvi Hong-Yan Zhang Ya-Wen He State Key Laboratory of Microbial Metabolism Joint International Research Laboratory of Metabolic and Developmental SciencesSJTU-Shanghai Nong Le Joint R&D Center on Biopesticides and BiofertilizersShanghai Jiao Tong University(SJTU)Shanghai200240China Department of Biology Faculty of ScienceKing Mongkut’s Institute of Technology LadkrabangBangkokThailand Department of Biotechnology COMSATS University IslamabadAbbottabad CampusAbbottabadPakistan Shanghai Nong Le Biological Products Company Limited Shanghai201419China

Histone-like nucleoid-structuring(H-NS)proteins are key regulators in gene expression silencing and in nucleoid *** H-NS family member proteins MvaU in Pseudomonas aeruginosa are thought to bind the same AT-rich regions of chromosomes and function to coordinate the control of a common set of ***,we explored the molecular mechanism by which MvaU controls PCA biosynthesis in *** *** present evidence suggesting that MvaU is *** of mvaU significantly increased PCA production,and PCA production sharply decreased when mvaU was *** transcriptionally repressed phz2 cluster expression and consequently reduced PCA biosynthesis.β-galactosidase assays confirmed that base pairing near the􀀀35 box is required when MvaU regulates PCA production in *** mobility shift assays(EMSA)and additional point mutation analysis demonstrated that MvaU directly bound to an AT-rich motif within the promoter of the phz2 *** immunoprecipitation(ChIP)analysis also indicated that MvaU directly bound to the P5 region of the phz2 cluster *** repression of PCA biosynthesis was independent of QscR and OxyR in PA1201 and neither PCA or H2O2 were the environmental signals that induced mvaU *** findings detail a new MvaU-dependent regulatory pathway of PCA biosynthesis in PA1201 and provide a foundation to increase PCA fermentation titer by genetic engineering.

关键词： Pseudomonas Phenazine-1-carboxylic acid phz cluster MvaU Regulatory network

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：