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Journal of Agricultural Science and Technology(A) 2013年第8期3卷 617-621页

作者： Ramesh Babu Veegala Shashi Vemuri chintalapati padmavathy Muthugounder Mohan Sena Balachandran AINP on Pesticide Residues Acharya N.G. Ranga Agricultural University Raj'endranagar Hyderabad 500030 India Directorate of Rice Research Rajendranagar Hyderabad 500030 India

Eight insecticidal crystal proteins of Bacillus thuringiensis, CrylAa, CrylAb, CrylAc, CrylB, Cry2Aa, CrylC, CrylDa and Cry 1Ea were assessed for toxicity against 1 st instar larvae of rice leaf folder, Cnaphalocrocis medinalis （Guenee） at 48 HAT and 72 HAT. Bioassay results depicted CrylAa was the most toxic （LCso 2.35 ppm） followed by CrylBa （LCso 8,50 ppm） and CrylAb （LCso 8.73 ppm） at 48 HAT, whereas, at 72 HAT CrylAb proved to be highly toxic （LC50 0.50 ppm） followed by CrylAa （LCso 4.07 ppm）, CrylAc （LCso 4,84 ppm） and CrylBa （LCso 6.42 ppm）. Toxins Cry2Aa, CrylCa, CrylDa and CrylEa did not resulted in any mortality at 48 HAT and 72 HAT, respectively. Baseline estimates for CrylAb against 1st instar larvae of C. medinalis sampled from seven geographical locations revealed variation in LC50＇s from 0.37 ppm to LC50 16.25 ppm at 48 HAT and LC50 0.50 ppm to LC50 6.49 ppm 72 HAT, respectively with relative resistance ratios of 44-fold and 13-fold at 48 HAT and 72 HAT over the susceptible population.

关键词： Bacillus thuringiensis rice leaf folder Cnaphalocrocis medinalis (Guenee) rice insecticidal crystal proteins.

Carboxylesterase and Glutathione-S-Transferase （GST＇s） Induced Resistance to Bacillus thuringiensis Toxin CrylAb in Rice Leaf Folder, Cnaphalocrocis medinalis （Guenee） Populations

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Journal of Agricultural Science and Technology(A) 2013年第1期3卷 53-59页

作者： Veegala Ramesh Babu Vemuri Shashi Bhushan chintalapati padmavathy: Muthugonder Mohan SenaMahendran. Balachandran Bellamkonda Ramesh AINP on Pesticide Residues Acharya N.G. Ranga Agricultural University Andhra Pradesh Rajendranagar Hyderabad 500030India Division of Crop Protection Directorate of Rice Research Rajendranagar Andhra Pradesh Hyderabad 500030 India Division of Crop Improvement Directorate of Rice Research Rajendranagar Andhra Pradesh Hyderabad 500030 India

The rice leaf folder （RLF）, Cnaphalocrocis medinalis （Guenee）（lnsecta： Lepidoptera： Pyralidae）, is an important pest, widely distributed in many rice growing areas of Asia. The over-use of broad-spectrum chemical insecticides has been cited as a major cause of outbreaks of C. medinalis as excessive spraying of insecticide disrupts natural biological control insecticides still remain the major control tactics against leaf folder. Carbofuran and fenthion, bendiocarb, acephate, carbosulfan, quinolphos, monocrotophos, phosphamidon and fenvalerate are the common ones used against rice leaf folder. Genetically, modified rice lines expressing B. thuringiensis insecticidal crystal proteins produced are highly tolerant to leidopteran pests. Though economic and environmental benefits of GM crops is well established, the matter of concern is the possibility of target insect pest developing resistance to this B. thuringiensis insecticidal toxins, evident from many laboratory and field experiments against many insect pests. The involvement of GSH S-transferase, carboxylesterase, and microsomal monooxygenase in insecticide resistance has been reported in insecticide-resistant strains of many insect species. Hence, the present study was taken up to monitor for cross resistance between B. thuringiensis cry toxins and synthetic insecticides in larvae of leaf folder as it is mediated by carboxylesterase titre and other enzymes by bioassay for two selected rice leaf folder field populations at the Entomology division of Directorate of Rice Research which showed 2-fold resistance ratio. Qualitative and quantitative changes of carboxylesterase （CarE） and glutathione-s-transferase （GST＇s） were worked out with midguts extracts of the two C. medinalis populations in the presence of a-napthyl acetate and chlorodi-nitro benzene substrates.

关键词： Cnaphalocrocis medinalis carboxylesterase and glutathione-s-transferase isozymes B type esterases.

Physiological Age Status of Female Adults and Off-Season Survival of Rice Leaffolder Cnaphalocrocis medinalis in India

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Rice science 2015年第5期22卷 237-244页

作者： Padmavathi chintalapati Katti GURURAJ Sailaja VALLABUNI Prasad YENUMULAG Directorate of Rice Research Central Research Institute for Dryland Agriculture

Rice leaffolder, Cnaphalocrocis medinalis, is one of the major foliage feeders found in the rice growing regions in India. When the crop was at maturity, numerous adults of rice leaffolder were found in the rice fields though the larval population gradually decreased, and no eggs were found on rice leaves. The population characteristics of C. medinalis were assessed based on the physiological age status of adults at different crop growth stages. Based on egg development within ovarioles, ovariole appearance number and colour of fat bodies, and characteristics of bursa copulatrix, physiological age status of the adults was described, which served as a basis for the determination of age composition. C. medinalis adults were found during the first week of August on rice plants, of which 44% were in Age 0 with immature ovaries, indicating immigrants. However, 28% adults each were at Ages 1-2 with developing ovaries, indicating local breeding population. The carryover and off-season survival of C. medinalis were also studied to determine the contribution of the alternative hosts in the population growth that helped in devising efficient management strategies. Rice was the most preferred host followed by Triticum aestivum, Echinochloa crusgulli and Brachiaria plantaginea. Various routes of the carryover of C. medinalis from season to season were discussed.

关键词： rice leaffolder physiological age carryover off-season survival alternative host ovariole weed

Theory of Deuteron and MCPE

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Journal of Modern Physics 2013年第9期4卷 1180-1184页

作者： chintalapati vavb Chandraraju Department of Physics Osmania University Hyderabad India

All the experimental values of the Deuteron nucleus except the magnetic moment are theoretically derived using the ordinary methods of quantum mechanics along with the morphed gravitational potential energy. To convince that the potential energy function used is indeed the right one, it is applied to determine the energy spectrums of the nuclei: 1) Triton, 2) helium-3, 3) lithium-7, 4) Beryllium-9, and 5) Beryllium-8. The Morphed Coulomb Potential Energy (MCPE) is also obtained. With the help of MCPE and the gravitational potential energy of the electron, the charge quantum number is obtained. For galaxies, the two dominant forces that are responsible for the expansion, contraction or stationary state of the universe are obtained.

关键词： Morphed Potential Energy Bindingenergy Radius Quadrupole Moment Superposed State Morphed Coulomb Potential Energy

Polarizabilities and Orientational Order Parameter in N-(p-n-Ethoxybenzylidene)- p-n-Alkoxy Anilines, *** LC Compounds

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Journal of Surface Engineered Materials and Advanced Technology 2016年第3期6卷 134-148页

作者： Putcha Srinivasa Sastry chintalapati Srinivas Pokkunuri Pardhasaradhi Venkata Gopala Krishna Murthy Pisipati Department of Physics Krishna University Machilipatnam India Department of Physics A. L. College Vijayawada India Liquid Crystal Research Centre Department of Electronics and Communications Engineering Koneru Lakshmaiah University Vaddeswaram India

n.m, ***, nO.m and *** compounds play an important role in the fundamental and applied aspects. As a part of our systematic studies of the above homologous series regarding the synthesis, characterization and phase transition studies, here the authors report the polarizabilities and orientational order parameter, S in N-(p-n-ethoxybenzylidene)-p-n-alkoxy anilines, *** with m = 3 to 10 liquid crystalline compounds except with m = 5 which has been already published. The rest of the compounds exhibit the nematic LC phase except with m = 10 which shows the smectic-C phase along the nematic phase which is originally not detected by Godzwon et al. But the author’s observations with TM and differential scanning calorimeter have shown the existence on SmC phase in addition to nematic phase. Further, SmC is clarified using the histogram technique whose details are given below and are published elsewhere. The orientstonal order parameter, S is estimated using different methods and compared with the value obtained from birefringence, δn = (ne – no) where no field model is used. It is observed in these compounds case as in other LC compounds Vuks isotropic model is favored compared to that of anisotropic model proposed by Neugebauer.

关键词： Orientstonal Order Parameter S 2O.Om LC Compounds Vuks Isotropic Model Neugebauer Anisotropic Model Nematic Phase