检索结果-南通市图书馆

American Journal of Plant Sciences 2016年第13期7卷 1802-1819页

作者： T. Vijayalakshmi A. S. Vijayakumar K. Kiranmai A. Nareshkumar chinta sudhakar Department of Botany Sri Krishnadevaraya University Anantapur India Department of Crop Physiology GKVK Bangalore India

Safflower is an important, traditional, multipurpose oil crop. This was to investigate the effect of different salinity levels on morphological, physiological, biochemical and antioxidant response of two safflower cultivars (Carthamus tinctorius L. cultivar TSF1 and cultivar SM) differing in salt tolerance. Salinity stress (0.0%, 1.0%, 1.5% and 2.0% of NaCl) was induced to safflower plants after 19 days of vegetative growth. After 12 days of stress impositions, plants were harvested and analysed for various parameters. The results revealed that cultivar TSF1 showed maximum growth, dry weight, cell membrane stability and more water content in both root and leaf tissues at higher salinity levels than cultivar SM. Salt stress resulted an accumulation of more soluble sugars, amino acids, proline and glycine betaine at high salinity level confers the tolerance potential of cultivar TSF1 over cultivar SM. Salt stress induces more increase in the enzyme activity of superoxide dismutase, ascorbate peroxidase, guaiacol peroxidase and catalase in tolerant cultivar than sensitive one. The results indicate that each cultivar adopt specific strategy at distinct salinity level for resistance against salinity. The possible conclusion is that improved tolerance in cultivar TSF1 to salinity may be accomplished by better management of growth, physiological attributes and antioxidative defence mechanisms.

关键词： Safflower Salinity Stress Compatible Solutes Antioxidative Enzymes

Expression Analysis of Aldo-Keto Reductase 1 (AKR1) in Foxtail Millet (Setaria italica L.) Subjected to Abiotic Stresses

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

American Journal of Plant Sciences 2016年第3期7卷 500-509页

作者： Tanguturi Venkata Kirankumar Kalaiahgari Venkata Madhusudhan Ambekar Nareshkumar Kurnool Kiranmai Uppala Lokesh Boya Venkatesh chinta sudhakar Plant Molecular Biology Laboratory Department of Botany Sri Krishnadevaraya University Anantapuramu India

Foxtail millet (Setaria italica L.) is a drought-tolerant millet crop of arid and semi-arid regions. Aldo-keto reductases (AKRs) are significant part of plant defence mechanism, having an ability to confer multiple stress tolerance. In this study, AKR1 gene expression was studied in roots and leaves of foxtail millet subjected to different regimes of PEG- and NaCl-stress for seven days. The quantitative Real-time PCR expression analysis in both root and leaves showed upregulation of AKR1 gene during PEG and salt stress. A close correlation exits between expression of AKR1 gene and the rate of lipid peroxidation along with the retardation of growth. Tissue-specific differences were found in the AKR1 gene expression to the stress intensities studied. The reduction in root and shoot growth under both stress conditions were dependent on stress severity. The level of lipid peroxidation as indicated by MDA formation was significantly increased in roots and leaves along with increased stress levels. Finally, these findings support the early responsive nature of AKR1 gene and seem to be associated at least in part with its ability to contribute in antioxidant defence related pathways which could provide a better protection against oxidative stress under stress conditions.

关键词： Aldo-Keto Reductase Lipid Peroxidation Reactive Carbonyls Cellular Homeostasis Plant Abiotic Stress Response

Pb-Stress Induced Oxidative Stress Caused Alterations in Antioxidant Efficacy in Two Groundnut (<i>Arachis hypogaea</i>L.) Cultivars

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Agricultural Sciences 2015年第10期6卷 1283-1297页

作者： Ambekar Nareshkumar Gounipalli Veeranagamallaiah Merum Pandurangaiah Kurnool Kiranmai Vennapusa Amaranathareddy Uppala Lokesh Boya Venkatesh chinta sudhakar Plant Molecular Biology Unit Department of Botany Sri Krishnadevaraya University Anantapur India

Lead (Pb) is an important environmental pollutant extremely toxic to plants and other living organisms including humans. To assess Pb phytotoxicity, a pot culture experiment was carried out using two groundnut cultivars (Arachis hypogaea L. cultivar K6 and cultivar K9) on plant growth, ROS levels, lipid peroxidation, and antioxidant metabolism using biochemical, histochemical methods. Plants were grown in pots for 14 days, in the botanic garden, and subjected to Pb-stress (0, 100, 200, 400 and 800 ppm) by adding Pb (NO3)2 solution and further allowed to grow for 10 days. The results showed that cultivar K6 registered lower Pb accumulation than cultivar K9, however, localization of Pb was greater in roots than leaves in both groundnut cultivars. The Pb-stress results in an increase in free radicals (O2?- and H2O2) generation in both groundnut cultivars, but more significantly in cultivar K9 than K6. Pb-stress also caused significant changes in the rate of peroxidation as shown in the levels of malondialdehyde (MDA) content in roots and leaves of both groundnut cultivars. Free proline, ascorbic acid (AsA) and non-protein thiol (NP-SH) contents were increased in cultivar K6 due to Pb-stress, but less in cultivar K9. Pb treated plants showed increased levels of antioxidant enzymes such as superoxide dismutase (SOD), guaiacol peroxidase (GPX), ascorbate peroxidase (APX) glutathione reductase (GR) and glutathione S-transferase (GST). Isozyme band intensities of SOD, GPX and APX were more consistent with the respective changes in antioxidative enzyme activities. These results indicate that cultivar K6 possesses greater tolerance potential for Pb toxicity than cultivar K9.

关键词： Groundnut (Arachis hypogaea L.) Pb-Stress Oxidative Stress Antioxidant Enzymes Non-Protein Thiols Reactive Oxygen Species

Expression Analysis of WRKY Transcription Factor Genes in Response to Abiotic Stresses in Horsegram (Macrotyloma uniflorum (Lam.) Verdc.)

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

American Journal of Molecular Biology 2016年第4期6卷 125-137页

作者： Kurnool Kiranmai Lokanadha Rao Gunupuru Ambekar Nareshkumar Vennapusa Amaranatha Reddy Uppala Lokesh Merum Pandurangaiah Boya Venkatesh Tanguturi Venkata Kirankumar chinta sudhakar Department of Botany Sri Krishnadevaraya University Anantapur India Molecular Plant-Microbe Interactions Laboratory University College Dublin Dublin Ireland Department of Crop Physiology GKVK Bangalore India Shenzhen Research Institute The Chinese University of Hong Kong Shenzhen China

Drought and salt stress are two major environmental constraints that limit the productivity of agriculture crops worldwide. WRKY transcription factors are the plant-specific transcription factors that regulate several developmental events and stress responses in plants. The WRKY domain is defined by a 60-amino acid conserved sequence named WRKYGQK at N-terminal and a Zinc Finger-like motif at the C-terminal. WRKY genes are known to respond several stresses which may act as negative or positive regulators. The function of most of the WRKY transcription factors from non-model plants remains poorly understood. This investigation shows the expression levels of eight WRKY transcription factor genes from horsegram plant under drought and salt stress conditions. The increase in mRNA transcript levels of WRKY transcription factor genes was found to be high in drought stressed plants compared to salt-stressed plants. The levels of MDA which indicates the lipid peroxidation were less in drought stress. More ROS is produced in salt stress conditions compared to drought. The results show that the expression of WRKY transcription factors in drought stress conditions is reducing the adverse effect of stress on plants. These results also suggest that, during abiotic stress conditions such as drought and salt stress, WRKY transcription factors are regulated at the transcription level.

关键词： Drought Salt Lipid Peroxidation Plant Abiotic Stress qRT-PCR Reactive Oxygen Species WRKY