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Cloning of artemisinin biosynthetic cDNAs and novel ESTs and quantification of low temperature-induced gene overexpression

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Science China(Life Sciences) 2008年第3期51卷 232-244页

作者： ZENG QingPing1, ZHAO Chang1, YIN LuLu1, YANG RuiYi1, ZENG XiaoMei1, HUANG Ying2, FENG LiLing3 & YANG XueQin3 1 Laboratory of Biotechnology, Tropical medicine Institute, Guangzhou University of chinese medicine, Guangzhou 510405, China 2 Guangzhou General Hospital, Guangzhou Military Area Command of chinese PLA, Guangzhou 510010, China 3 Center of Artemisinin Research, Guangzhou University of chinese medicine, Guangzhou 510405, China Laboratory of Biotechnology Tropical Medicine Institute Guangzhou University of Chinese Medicine Guangzhou China Guangzhou General Hospital Guangzhou Military Area Command of Chinese PLA Guangzhou China Center of Artemisinin Research Guangzhou University of Chinese Medicine Guangzhou China

To isolate and verify novel genes from qinghao (Artemisia annua) based on the development-specific and environment-induced transcriptomics, leaves have been harvested from the flowering A. annua plants and exposed to low temperature for isolation of total RNAs and cloning of full-length cDNAs and cDNA fragments, or expressed sequence tags (ESTs). After being sequenced and browsed for homol- ogy, these sequences have been submitted to GenBank. Among the accessed 75 sequences, 4 full-length cDNAs are highly homologous to the known A. annua genes, but 71 ESTs are absent in the sequence records of A. annua genes, in which 34 sequences are homologous to other plant genes, including 24 identified protein-coding sequences and 10 unidentified protein-coding sequences, while other 37 sequences are not present in the sequence records of any plant genes, representing the first cloned plant genes. In order to investigate the responsive patterns of A. annua genes to extreme envi- ronmental stresses, especially low temperature, the expression levels of 3 critical qinhaosu (artemisi- nin) biosynthetic genes, ADS, CYP71AV1 and CPR, have been measured in pre- and post-chilling A. annua seedlings cultured in vitro by semi-quantitative PCR (SQ-PCR). Consequently, ADS and CYP71AV1 genes are strongly induced by chilling, but CPR gene is not significantly affected by such treatment. Furthermore, induction of these genes by chilling can be potently suppressed by Ca2+ channel inhibitor LaCl3 or Ca2+ chelator EGTA, suggesting a putative involvement of Ca2+-CaM signal transduction pathway in chilling-induced overexpression of ADS and CYP71AV1 genes. The real-time fluorescent quantitative PCR (RFQ-PCR) assay of A. annua seedlings exposed to chilling has shown that the expression level of CaM gene is up-regulated for more than 2.5 folds, thereby confirming our above inference on the relevance of Ca2+-CaM-mediated signal transduction to chilling-induced gene overexpression. Finally, 7 newly iso

关键词： Artemisia annua expressed sequence tag (EST) abiotic stress signal transduction fluorescent quantification

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《早产／低出生体重儿喂养建议》解读

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中华儿科杂志 2009年第7期47卷 513-516页

作者：王丹华中国医学科学院北京协和医学院北京协和医院儿科 100710

一、背景近年来随着医学科学的进步和新生儿重症监护技术的发展，早产儿的抢救成功率逐年提高，越来越多的极超低出生体重儿得以存活，而目前早产儿宫外生长迟缓（extrauterine growth restriction，EUGR）的状况已引起国内外学者的高... 详细信息

一、背景近年来随着医学科学的进步和新生儿重症监护技术的发展，早产儿的抢救成功率逐年提高，越来越多的极超低出生体重儿得以存活，而目前早产儿宫外生长迟缓（extrauterine growth restriction，EUGR）的状况已引起国内外学者的高度重视，

关键词：超低出生体重儿早产儿解读喂养重症监护技术抢救成功率医学科学生长迟缓

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Cytotoxicity of carbon nanotubes

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Science China Chemistry 2008年第11期51卷 1021-1029页

作者： ZHU Ying1,2 & LI WenXin1 1 Laboratory of Nano-biology and medicine, Shanghai Institute of Applied Physics, chinese Academy of Sciences, Shanghai 201800, China 2 Graduate School of chinese Academy of Sciences, Beijing 100049, China Laboratory of Nano-biology and medicine Shanghai Institute of Applied Physics Chinese Academy of Sciences Shanghai China Graduate School of chinese Academy of Sciences Beijing China

With large-scale production and application at large scale, carbon nanotubes (CNTs) may cause ad-verse response to the environment and human health. Thus, study on bio-effects and safety of CNTs has attracted great attention from scientists and governments worldwide. This report briefly summa-rizes the main results from the in vitro toxicity study of CNTs. The emphasis is placed on the descrip-tion of a variety of factors affecting CNTs cytotoxicity, including species of CNTs, impurities contained, lengths of CNTs, aspect ratios, chemical modification, and assaying methods of cytotoxicity. However, experimental information obtained thus far on CNTs' cytotoxicity is lacking in comparability, and some-times there is controversy about it. In order to assess more accurately the potential risks of CNTs to human health, we suggest that care should be taken for issues such as chemical modification and quantitative characterization of CNTs in cytotoxicity assessment. More importantly, studies on physical and chemical mechanisms of CNTs' cytotoxicity should be strengthened; assaying methods and evaluating criteria characterized by nanotoxicology should be gradually established.

关键词： carbon nanotubes (CNTs) cell bio-security

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重症急性胰腺炎镇痛治疗中国专家共识(2022版)

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中华消化外科杂志 2022年第12期21卷 1499-1509页

作者：亚洲急危重症协会中国腹腔重症协作组蒋正英郭丰李维勤不详重庆大学附属肿瘤医院重症医学科重庆400030 浙江大学医学院附属邵逸夫医院重症医学科杭州310016 中国人民解放军东部战区总医院重症医学科南京210002

重症急性胰腺炎中,镇痛治疗除了能缓解疼痛,还可能影响患者预后。因此,镇痛治疗作为重症急性胰腺炎综合治疗的一部分,越来越受到重视。然而,重症急性胰腺炎患者的镇痛治疗仍存在诸多挑战,且目前没有共识性的建议。亚洲急危重症协会中国... 详细信息

重症急性胰腺炎中,镇痛治疗除了能缓解疼痛,还可能影响患者预后。因此,镇痛治疗作为重症急性胰腺炎综合治疗的一部分,越来越受到重视。然而,重症急性胰腺炎患者的镇痛治疗仍存在诸多挑战,且目前没有共识性的建议。亚洲急危重症协会中国腹腔重症协作组专家针对重症急性胰腺炎中疼痛和镇痛治疗对疾病和预后的影响、镇痛药物和镇痛方式选择等方面讨论并提出21条临床最关注的问题,综合循证医学证据以及临床经验制订《重症急性胰腺炎镇痛治疗中国专家共识(2022版)》,旨在为临床医师提供参考和建议,以期改善患者预后。

关键词：重症急性胰腺炎疼痛镇痛重症监护共识

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Applications of critical ultrasonography in hemodynamic therapy

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chinese Medical Journal 2021年第11期134卷 1286-1288页

作者： Wei Huang Da-Wei Liu Xiao-Ting Wang chinese critical Ultrasound Study Group(CCUSG) Department of critical care medicine Peking Union Medical College HospitalPeking Union Medical CollegeChinese Academy of Medical SciencesBeijing 100730China 不详

critical ultrasonography(CUS)is used in almost every branch of critical care medicine because of rapid development of this *** enables goal-directed,continuous,and dynamic *** use of CUS in hemodynamic therapy provides a detailed understanding of hemodynamics and optimizes hemodynamic *** article describes the current applications of CUS in hemodynamic therapy from various aspects.

关键词： ultrasonography hemodynamic technology.

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Expert Consensus on the Diagnosis and Treatment of Anticancer Drug-Induced Interstitial Lung Disease

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Current Medical Science 2023年第1期43卷 1-12页

作者：无 Fei MA Hua-ping DAI Anticancer Drug-induced Interstitial Lung Disease Diagnosis and Treatment Group China Interstitial Lung Diseases Panel Chinese Medical Association-Chinese Thoracic Society and Chinese Medical Doctor Association-Chinese Association of Chest PhysiciansChina Department of Medical Oncology National Cancer CenterNational Clinical Research Center for CancerCancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 10021China Department of Pulmonary and critical care medicine China-Japan Friendship HospitalNational Center for Respiratory MedicineChinese Academy of Medical SciencesBeijing 100029China

Drug-induced interstitial lung disease(DILD)is the most common pulmonary adverse event of anticancer *** recent years,the incidence of anticancer DILD has gradually increased with the rapid development of novel anticancer *** to the diverse clinical manifestations and the lack of specific diagnostic criteria,DILD is difficult to diagnose and may even become fatal if not treated ***,a multidisciplinary group of experts from oncology,respiratory,imaging,pharmacology,pathology,and radiology departments in China has reached the“expert consensus on the diagnosis and treatment of anticancer DILD”after several rounds of a comprehensive *** consensus aims to improve the awareness of clinicians and provide recommendations for the early screening,diagnosis,and treatment of anticancer *** consensus also emphasizes the importance of multidisciplinary collaboration while managing DILD.

关键词： drug-induced interstitial lung disease anticancer drug diagnosis treatment

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老年重症患者静脉血栓栓塞症预防中国专家共识(2023)

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中华危重病急救医学 2023年第6期35卷 561-572页

作者：中国老年医学学会重症医学分会浙江省重症医学临床医学研究中心严静李莉钱筱玲周鑫飞不详浙江医院重症医学科杭州310013

重症患者是静脉血栓栓塞症(VTE)的高危人群,患者年龄越大,VTE发生率越高。尽管VTE预后差,但VTE是可以预防的。目前,国内外虽然已有多个关于VTE预防的共识或指南,但尚缺少针对老年重症患者VTE预防的共识或指南。为了规范我国老年重症患者... 详细信息

重症患者是静脉血栓栓塞症(VTE)的高危人群,患者年龄越大,VTE发生率越高。尽管VTE预后差,但VTE是可以预防的。目前,国内外虽然已有多个关于VTE预防的共识或指南,但尚缺少针对老年重症患者VTE预防的共识或指南。为了规范我国老年重症患者的VTE预防,由中国老年医学学会重症医学分会和浙江省重症医学临床医学研究中心牵头制定了《老年重症患者静脉血栓栓塞症预防中国专家共识(2023)》。本共识工作组成员查阅国内外相关指南、综合循证医学依据和临床经验,形成共识初稿,递交专家组各位专家多次讨论,历经多次修订,最后以电子问卷形式发给专家组,专家根据共识条目的理论依据、科学性、可行性等综合评分,确定各条目的推荐强度,最终形成21条推荐意见,为老年重症患者VTE预防提供了参考依据。

关键词：静脉血栓栓塞症老年重症专家共识

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CSOG MFM Committee Guideline:Clinical Management Guidelines for Acute Fatty Liver of Pregnancy in China(2021)

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Maternal-Fetal medicine 2021年第4期3卷 238-245页

作者： Maternal-Fetal medicine Committee,chinese society of Obstetrics and Gynecology,chinese Medical Ping Li Yaolong Chen Weishe Zhang Huixia Yang 不详 Department of Obstetrics Xiangya Hospital Central South UniversityChangshaHunan 410008China Hunan Engineering Research Center of Early Life Development and Disease Prevention ChangshaHunan 410008China Evidence-Based medicine Center School of Basic Medical SciencesLanzhou UniversityLanzhouGansu 730000China chinese GRADE Center LanzhouGansu 730000China Department of Obstetrics and Gynecology Peking University First HospitalBeijing 100034China Beijing Key Laboratory of Maternal Fetal medicine of Gestational Diabetes Mellitus Beijing 100034China

Acute fatty liver of pregnancy(AFLP)is a rare but critical obstetric-specific disease with a high fatality rate,posing a serious threat to the safety of mothers and *** guidelines were specially formulated to standardize AFLP clinical pathways and to improve maternal and infant *** on a two-round questionnaire survey,the guideline development team identified the following nine clinical issues that clinicians were most concerned about,and developed recommendations for each of them:prenatal outpatient screening for AFLP,diagnosis,preoperative risk assessment,delivery modes and timing,anesthesia methods,perinatal complications,selecting AFLP patients for artificial liver treatment,prognostic assessment,and monitoring during *** guidelines cover the key issues related to AFLP diagnosis and treatment that concern clinicians.

关键词： Fatty liver Acute fatty liver of pregnancy Guidelines Classification of evidence quality and strength of recommendation

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SHP2 deletion in airway epithelium reduces allergen induced the airway hyperresponsiveness

SHP2 deletion in airway epithelium reduces allergen induced ...

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中华医学会呼吸病学年会——2011（第十二次全国呼吸病...

作者： Zhangwei Qiu,Xuejun Qin,Wen Li,Zhihua Chen,Huahao Shen Department of Respiratory and critical care medicine,Second Affiliated Hospital of Zhejiang University School of medicine

SHP2,as a member of nonreceptor protein tyrosine phosphatase,is important in embryonic lung branching morphogenesis,and is involved in the regulation of immune ***, whether SHP2 regulates the pathology of allergic asthma still remains *** we generated airway epithelium specific Shp2 deficient mice,and use these mice to explore the

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Functional mitral regurgitation combined with increased early diastolic transmitral velocity to early mitral annulus diastolic velocity ratio is associated with a poor prognosis in patients with shock

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chinese Medical Journal 2021年第19期134卷 2299-2305页

作者： Ran Zhou Tongjuan Zou Wanhong Yin Xiaoting Wang Yan Kang chinese critical Ultrasound Study Group(CCUSG) Department of critical care medicine West China Hospital/West China School of MedicineSichuan UniversityChengduSichuan 610041China Department of critical care medicine Peking Union Medical College HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing100730China 不详

Background:Functional mitral regurgitation(FMR)is common in critically ill patients and may cause left atrial(LA)pressure *** study aims to explore the prognostic impact of synergistic LA pressure elevation and FMR in patients with ***:We retrospectively screened 130 consecutive patients of 175 patients with shock from April 2016 to June *** incidence and impact of FMR and early diastolic transmitral velocity to early mitral annulus diastolic velocity ratio(E/e’)≥4 within 6 h of shock on the prognosis of patients were ***,the synergistic effect of FMR and E/e’were assessed by combination,grouping,and trend ***:Forty-four patients(33.8%)had FMR,and 15 patients(11.5%)had E/e’elevation.A multivariate analysis revealed FMR and E/e’as independent correlated factors for 28-day mortality(P=0.043 and 0.028,respectively).The Kaplan-Meier survival analysis revealed a significant difference in survival between patients with and without FMR(χ2=7.672,P=0.006)and between the E/e’≥14 and E/e’<14 groups(χ2=19.351,P<0.010).Twenty-eight-day mortality was significantly different among the four groups(χ2=30.141,P<0.010).The risk of 28-day mortality was significantly higher in group 4(E/e’≥14 with FMR)compared with groups 1(E/e’<14 without FMR)and 2(E/e’<14 with FMR)(P=0.001 and 0.046,respectively).Conclusions:Patients with shock can be identified by the presence of *** and E/e’are independent risk factors for a poor prognosis in these patients,and prognosis is worst when FMR and E/e’≥14 are *** may be possible to improve prognosis by reducing LA pressure and E/e’.Trial Registration:***,NCT03082326.

关键词： Shock Ultrasound Functional mitral regurgitation E/e’ Prognosis

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