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Natural Resources 2012年第4期3卷 175-179页

作者： chikako asada Keita Doi Chizuru Sasaki Yoshitoshi Nakamura Department of Life System Institute of Technology and Science University of Tokushima Tokushima Japan

To utilize starch and protein contained in microalgae as carbon and nitrogen sources for ethanol production, an extraction method, i.e. ultrasonic treatment using a homogenizer, and simultaneous saccharification and fermentation (SSF) of extracted microalgae solution were studied using Chlamydomonas fasciata Ettl 437. 30 min of ultrasonic treatment gave the maximum extraction ratio of starch contained in microalgae, i.e. 93.8%, that corresponded to 0.408 g-starch/g-dry microalgae. SSF of the extracted solution obtained from ultrasonic treated microalgae at 30 min by glutase-AN and Saccahromyces cerevisiae AM12 provided 0.194 and 0.168 g-ethanol/g-dry microalgae with and without yeast extract, respectively, corresponding to 79.5 and 68.8% of theoretical ethanol yield.

关键词： Bioethanol Microalgae Ultrasonic Treatment Homoginizer Starch

Acceleration of <i>Hericium erinaceum</i>mycelial growth in submerged culture using yogurt whey as an alternative nitrogen source

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Advances in Bioscience and Biotechnology 2012年第7期3卷 828-832页

作者： chikako asada Ryosuke Okumura Chizuru Sasaki Yoshitoshi Nakamura Department of Life System Institute of Technology and Science The University of Tokushima Tokushima Japan

The effects of various carbon sources and their initial concentrations on mycelia production by Hericium erinaceum were investigated by determining the dry cell weight (DCW) and β-glucan content of mycelia in submerged culture. Glucose and xylose were superior carbon sources for promoting mycelial growth resulting in mycelial concentrations of 3.99 g/L and 4.01 g/L, respectively;glucose was the best carbon source in terms of productivity (0.44 g/L/day). Experiments were also performed using yogurt whey as an alternative nitrogen source for submerged cultivation of H. erinaceum mycelia, and DCW and β-glucan content were compared with those with chemical nutrient medium. When whey was used as a nitrogen source, DCW and total amount of β-glucan were 2.3- and 2.8-fold higher, respectively, than that with chemical nutrient medium. Thus, whey appears to be an alternative nitrogen source for promoting H. erinaceum mycelial growth.

关键词： Hericium Erinaceum Mycelia Submerged Culture Yogurt Whey

Asking one mechanism in glial cells during neuroinflammation

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Neural Regeneration Research 2025年第4期20卷 1077-1078页

作者： Xiaoli Guo chikako Harada Takayuki Harada Visual Research Project Tokyo Metropolitan Institute of Medical ScienceTokyoJapan

Multiple sclerosis(MS),which is characterized by inflammatory demyelination in the central nervous system(CNS),is the most common neurological disease in the young adult *** autoimmune encephalomyelitis(EAE),an animal model of MS,is often used in preclinical *** data indicate that in addition to immune cells such as T cells and dendritic cells,CNS resident microglia and astrocytes play important roles in demyelinating neuroinflammation(Healy et al.,2022).In particular,microglia are key immune-competent cells that can respond to environmental *** depletion of transforming growth factor-β-activated kinase 1,a mitogen-associated protein kinase kinase kinase,in microglia is reported to reduce CNS inflammation and diminish axonal and myelin damage *** suggests that elucidating the mechanisms of microglia-specific responses during pathologies may help in the development of treatments that reduce EAE/MS disease severity(Goldmann et al.,2013).

关键词： inflammation clinical

Monogenic gene therapy for glaucoma and optic nerve injury

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Neural Regeneration Research 2025年第3期20卷 815-816页

作者： chikako Harada Xiaoli Guo Takayuki Harada Visual Research Project Tokyo Metropolitan Institute of Medical ScienceTokyoJapan

The prevalence of glaucoma, the second leading cause of global blindness, is increasing due to aging populations. In glaucoma, degeneration of the optic nerve and retinal ganglion cells(RGCs) causes visual field defec... 详细信息

关键词： visual glaucoma degeneration

Stem cell therapy for central nerve system injuries: glial cells hold the key

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Neural Regeneration Research 2014年第13期9卷 1253-1260页

作者： Li Xiao chikako Saiki Ryoji Ide Pharmacology Department The Nippon Dental University School of Life Dentistry at Tokyo Tokyo Japan Physiology Department The Nippon Dental University School of Life Dentistry at Tokyo Tokyo Japan

Mammalian adult central nerve system （CNS） injuries are devastating because of the intrinsic difficulties for effective neuronal regeneration. The greatest problem to be overcome for CNS recovery is the poor regeneration of neurons and myelin-forming cells, oligodendrocytes. Endogenous neural progenitors and transplanted exogenous neuronal stem cells can be the source for neuronal regeneration. However, because of the harsh local microenvironment, they usually have very low efficacy for functional neural regeneration which cannot compensate for the loss of neurons and oligodendrocytes. Glial cells （including astrocytes, microglia, oligodendrocytes and NG2 glia） are the majority of cells in CNS that provide support and protection for neurons. Inside the local microenvironment, glial cells largely influence local and transplanted neural stem cells survival and fates. This review critically analyzes current finding of the roles of glial cells in CNS regeneration, and highlights strategies for regulating glial cells＇ behavior to create a permissive microenvironment for neuronal stem cells.

关键词： Neuron regeneration stem cell therapy glial cells microenvironment oligodendrocyteregeneration CNS injury

Serrated flow accompanied with dynamic type transition of the Portevin-Le Chatelier effect in austenitic stainless steel

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Journal of Materials Science & Technology 2023年第2期133卷 154-164页

作者： Seung-Yong Lee Sita Chettri Ritupan Sarmah chikako Takushima Jun-ichi Hamada Nobuo Nakada Department of Materials Science and Engineering Tokyo Institute of Technology4259 Nagatsuta-choMidori-kuYokohama-shiKanagawa-ken 226-8503Japan Department of Physics Tezpur UniversityNappamTezpur 784028India Research and Development Center Nippon Steel Stainless Steel CorporationHikari 743-8550Japan

In order to further understand high-temperature deformation behavior and its-related mechanical prop-erties,the Portevin-Le Chatelier(PLC)effect in an Fe-19Cr-13Ni-0.2C austenitic stainless steel was in-vestigated using high-temperature digital image correlation(DIC)*** the tensile testing at temperatures from 473 to 623 K,different types of serrated flow appeared even at a constant applied strain rate,and the type transition took place dynamically in a certain order with deformation *** analysis revealed that the dynamic type transition of the serrated flow obeys the PLC band propagation behavior,and that the transition of the PLC band propagation behavior could be attributed to the PLC band nucleation *** modeling also proved that the nucleation manner of PLC band nu-cleation is determined by the spatial-temporal coupling effect.

关键词： Dynamic strain aging(DSA) Austenitic stainless steel(γ-STS) Portevin-Le Chatelier(PLC)effect Digital image correlation(DIC) Local strain distribution

Enzyme-digested Colla Corii Asini(E’jiao) prevents hydrogen peroxide-induced cell death and accelerates amyloid beta clearance in neuronal-like PC12 cells

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Neural Regeneration Research 2020年第12期15卷 2270-2277页

作者： Li Xiao Feng Liao Ryoji Ide Tetsuro Horie Yumei Fan chikako Saiki Nobuhiko Miwa Department of Pharmacology The Nippon Dental UniversitySchool of Life Dentistry at TokyoTokyoJapan National Engineering Research Center for Gelatin-based Traditional Chinese Medicine Dong-E-E-Jiao Co.Ltd.LiaochengShandong ProvinceChina Department of Physiology The Nippon Dental UniversitySchool of Life Dentistry at TokyoTokyoJapan Research Center The Nippon Dental UniversitySchool of Life Dentistry at TokyoTokyoJapan Department of Life Sciences Prefectural University of HiroshimaHiroshimaJapan

As an aging-associated degenerative disease,Alzheimer’s disease is characterized by the deposition of amyloid beta(Aβ),oxidative stress,inflammation,dysfunction and loss of cholinergic *** Corii Asini(CCA)is a traditional Chinese medicine which has been used for feebleness-related diseases and *** might delay aging-induced degenerative changes in *** the present study,we evaluated antioxidant activity,cytoprotective effects,and Aβremovability of enzyme-digested Colla Corii Asini(CCAD).Oxygen radical absorbance capacity(ORAC)activity assay showed that,as compared to gelatins from the skin of porcine,bovine and cold water fish,CCA exhibited the highest ORAC *** ORAC activity of CCA and CCAD was increased gradually by the length of time in *** analysis by scanning electron microscopy showed that among CCA manufactured in 2008,2013,2017 and gelatin from cold water fish skin,CCA manufactured in 2008 presented the smoothest surface *** further tested the protective effects of CCAD(manufactured in 2008)and enzyme-digested gelatin from cold water fish skin(FGD)on hydrogen peroxide(H2O2)-induced cell death in nerve growth factor-differentiated neuronal-like PC12 *** blue assay showed that both FGD and CCAD at 0.5 mg/m L increased cell viability in H2O2-treated neuronal-like PC12 *** protection of CCAD was significantly superior to that of ***(Ach E)assay showed that both FGD and CCAD inhibited Ach E activity in nerve growth factor-differentiated neuronal-like PC12 cells to 89.1%and 74.5%of that in non-treated cells,*** data suggest that CCAD might be able to increase the neurotransmitter *** CCAD inhibited Ach E activity in neuronal-like PC12 cells,CCAD prevented H2O2-induced abnormal deterioration of Ach *** and neprilysin activity assay results indicated that CCAD reduced amyloid beta accumulation and increased neprilysin activity in Aβ1–

关键词： acetylcholinesterase activity Alzheimer’s disease amyloid beta clearance antioxidant Colla Corii Asini collagen neuroprotection PC12 cells

Genetic variants involved in gallstone formation and capsaicin metabolism,and the risk of gallbladder cancer in Chilean women

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World Journal of Gastroenterology 2010年第3期16卷 372-378页

作者： Sergio Báez Yasuo Tsuchiya Alfonso Calvo Martha Pruyas Kazutoshi Nakamura chikako Kiyohara Mari Oyama Masaharu Yamamoto Department of Surgery Sótero del Río Hospital Department of Community Preventive Medicine Niigata University Graduate School of Medical and Dental Sciences Department of Anatomic Pathology Sótero del Río Hospital Department of Preventive Medicine Graduate School of Medical SciencesKyushu University Faculty of Health Sciences Niigata University of Health and Welfare

AIM:To determine the effects of genetic variants associated with gallstone formation and capsaicin (a pungent component of chili pepper) metabolism on the risk of gallbladder cancer (GBC).METHODS: A total of 57 patients with GBC, 119 patients with gallstones, and 70 controls were enrolled in this study. DNA was extracted from their blood or paraffi n block sample using standard commercial kits. The statuses of the genetic variants were assayed using Taqman SNP Genotyping Assays or Custom Taqman SNP Genotyping ***:The non-ancestral T/T genotype of apolipoprotein B rs693 polymorphism was associated with a decreased risk of GBC (OR:0.14,95% CI:0.03-0.63). The T/T genotype of cholesteryl ester transfer protein (CETP) rs708272 polymorphism was associated with an increased risk of GBC (OR:5.04,95% CI:1.43-17.8).CONCLUSION: Genetic variants involved in gallstone formation such as the apolipoprotein B rs693 and CETP rs 708272 polymorphisms may be related to the risk of developing GBC in Chilean women.

关键词： Genetic risk factor Gallbladder cancer Gallstone Genetic polymorphism Apolipoprotein B Cholesteryl ester transfer protein

The Mechanism of Kansei and the Activity of Brain on Appreciation of Art：The approach by measuring the activity of brain on appreciation of sculpture

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设计 2006年第F2期22卷 4-6页

作者： chikako YAMANE University of Tsukuba

Suppression of fibrosis in human pterygium fibroblasts by butyrate and phenylbutyrate

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International Journal of Ophthalmology(English edition) 2017年第9期10卷 1337-1343页

作者： Yuka Koga Noriaki Maeshige Hiroto Tabuchi Mikiko Uemura Michiko Aoyama-Ishikawa Makoto Miyoshi chikako Katakami Makoto Usami Division of Nutrition and Metabolism Department ofBiophysicsKobe University Graduate School of Health SciencesTomogaoka 7-10-2Suma-kuKobeJapan Department of Rehabilitation Science Kobe University Graduate School of Health SciencesTomogaoka 7-10-2Suma-kuKobeJapan Department of Ophthalmology Saneikai Tsukazaki HospitalWaku 68-1Aboshi-kuHimejiJapan Department of Nutrition Kobe University HospitalKobe University School of MedicineKusunoki-cho 7-5-2Chuo-kuKobeJapan

AIM：To evaluate the antifibrogenic effects of butyrate or phenylbutyrate,a chemical derivative of butyrate,in human pterygium ***：Human pterygium fibroblasts obtained from patient pterygium tissue were treated with butyrate or phenylbutyrate for *** ofα-smooth muscle actin,collagen I,collagen III and matrix metalloproteinase-1m RNA was measured by quantitative real-time reverse transcription polymerase chain reaction,and acetylated histone was evaluated by Western ***：Butyrate inhibitedα-smooth muscle actin,type III collagen and matrix metalloproteinase-1 expressions,and phenylbutyrate inhibited types I and III collagen and matrix metalloproteinase-1 expressions without changing cell viability as well as both of these increased histone *** results suggested that butyrate and phenylbutyrate suppress fibrosis through a mechanism involving histone deacetylase ***：This indicates that butyrate or phenylbutyrate have antifibrogenic effects in human pterygium fibroblasts and could be novel types of prophylactic and/or therapeutic drugs for pterygium,especially phenylbutyrate,which does not have the unpleasant smell associated with butyrate.

关键词： butyrate phenylbutymte pterygium fibroblasts antifibrogenic effect