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Induction of CD69 expression by cag PAI-positive Helicobacter pylori infection

World Journal of Gastroenterology 2011年第32期17卷 3691-3699页

作者： Naoki Mori chie ishikawa Masachika Senba Department of Microbiology and Oncology Graduate School of Medicine University of the Ryukyus Nishihara Okinawa 903-0215 Japan Department of Internal Medicine Omoromachi Medical Center 1-3-1 Uenoya Naha Okinawa 900-0011 Japan Transdisciplinary Research Organization for Subtropics and Island Studies University of the Ryukyus Nishihara Okinawa 903-0213 Japan Department of Pathology Institute of Tropical Medicine Nagasaki University Nagasaki 852-8523 Japan

AIM: To investigate and elucidate the molecular mechanism that regulates inducible expression of CD69 by Helicobacter pylori (H. pylori ) infection. METHODS: The expression levels of CD69 in a T-cell line, Jurkat, primary human peripheral blood mononuclear cells (PBMCs), and CD4 + T cells, were assessed by immunohistochemistry, reverse transcription polymerase chain reaction, and flow cytometry. Activation of CD69 promoter was detected by reporter gene. Nuclear factor (NF)-κB activation in Jurkat cells infected with H. pylori was evaluated by electrophoretic mobility shift assay. The role of NF-κB signaling in H. pylori-induced CD69 expression was analyzed using inhibitors of NF-κB and dominant-negative mutants. The isogenic mutants with disrupted cag pathogenicity island (cag PAI) and virD4 were used to elucidate the role of cag PAI-encoding type Ⅳ secretion system and CagA in CD69 ***: CD69 staining was detected in mucosal lymphocytes and macrophages in specimens of pa- tients with H. pylori-positive gastritis. Although cag PAI- positive H. pylori and an isogenic mutant of virD4 induced CD69 expression, an isogenic mutant of cag-PAI failed to induce this in Jurkat cells. H. pylori also induced CD69 expression in PBMCs and CD4 + T cells. The activation of the CD69 promoter by H. pylori was mediated through NF-κB. Transfection of dominant-negative mutants of IκBs, IκB kinases, and NF-κB-inducing kinase inhibited H. pylori-induced CD69 activation. Inhibitors of NF-κB suppressed H. pylori-induced CD69 mRNA expression. CONCLUSION: The results suggest that H. pylori induces CD69 expression through the activation of NF-κB. cagPAI might be relevant in the induction of CD69 expression in T cells. CD69 in T cells may play a role in H. pylori-induced gastritis.

关键词： CD69 T cells Helicobacter pylori cag pathogenicity island Nuclear factor-κB

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Beneficial effects of fucoidan in patients with chronic hepatitis C virus infection

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World Journal of Gastroenterology 2012年第18期18卷 2225-2230页

作者： Naoki Mori Kazunori Nakasone Koh Tomimori chie ishikawa Department of Microbiology and Oncology Graduate School of MedicineUniversity of the RyukyusNishiharaOkinawa 903-0215Japan Nakasonekazu Medical Clinic NahaOkinawa 902-0062Japan Department of Psychiatry Naha City HospitalNahaOkinawa 902-8511Japan Transdisciplinary Research Organization for Subtropics and Island Studies University of the RyukyusNishiharaOkinawa 903-0213Japan

AIM:To evaluate the effects of fucoidan,a complex sulfated polysaccharide extract from marine seaweed,on hepatitis C virus(HCV) RNA load both in vitro and in ***:HCV-1b replicon-expressing cells were cultured in the presence of fucoidan obtained from Cladosiphon okamuranus Tokida cultivated in Okinawa,Japan,and quantified the level of HCV *** an open-label uncontrolled study,15 patients with chronic hepatitis C,and HCV-related cirrhosis and hepatocellular carcinoma were treated with fucoidan(0.83 g/d) for 12 *** clinical symptoms,biochemical tests,and HCV RNA levels were assessed before,during,and after ***:Fucoidan dose-dependently inhibited the expression of HCV *** 8-10 mo of treatment with fucoidan,HCV RNA levels were significantly lower relative to the *** same treatment also tended to lower serum alanine aminotransferase levels,and the latter correlated with HCV RNA ***,the improved laboratory tests did not translate into significant clinical *** had no serious adverse ***:Our findings suggest that fucoidan is safe and useful in the treatment of patients with HCVrelated chronic liver *** controlled clinical trials are needed to confirm the present findings.

关键词： Fucoidan Hepatitis C virus Replicon

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Surgical Treatment of Lung Cancer in Patients with Psychiatric Disorders: A Retrospective Study

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Journal of Cancer Therapy 2016年第8期7卷 553-557页

作者： chie Ushijima Koji Yamazaki Hidenori Kouso Masakazu Katsura Ryo Mori Sadanori Takeo Kensuke ishikawa Department of General Thoracic Surgery and Clinical Research Institute National Hospital Organization Kyushu Medical Center Fukuoka Japan Department of Psychiatry National Hospital Organization Kyushu Medical Center Fukuoka Japan

Background: The purpose of this retrospective study was to examine the clinical outcomes of patients with concomitant lung cancer and psychiatric disorders who underwent pulmonary resection at our institution and develop a strategy for treating such patients in the future. Methods: We retrospectively reviewed 23 patients with psychiatric disorders who underwent pulmonary resection for lung cancer at our institution between January 1984 and December 2011. Results: The study population comprised 13 men and 10 women with an average age of 67.4 years. The histological types of lung cancer included adenocarcinoma in 14 patients, squamous cell carcinoma in 4, and other types in 5. Fifteen patients were classified as having pathological TNM stage I cancer, four as having stage II, three as having stage III, and one as having stage IV. The coexisting psychiatric disorders were neurosis in eight patients, depression in seven, schizophrenia in five, dementia in two, and oligophrenia in one. Three patients were admitted without a full understanding of the operation. There were no major complications with the exception of delirium, which was seen in eight patients. None of these patients’ coexisting psychiatric disorders worsened. No perioperative death occurred. Conclusions: At our institution, we cooperate closely with psychiatrists to care for surgical patients with psychiatric disorders. There is a possibility that such cooperation will reduce the frequency of previously reported complications.

关键词： Lung Cancer Surgery Psychiatric Disorder Surgery Incisions Exposure Techniques

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ULF Geomagnetic Anomalies Possibly Due to Crustal Activities in Boso and Izu Peninsula,Japan

ULF Geomagnetic Anomalies Possibly Due to Crustal Activities...

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中国地球物理学会第二十六届年会、中国地震学会第十三次学术大会

作者： Katsumi Hattori Takuya Hirano Hisashi ishikawa chie Yoshino Yuki Ishiguro Graduate School of Science Chiba UniversityJapan

*** A number of case studies have shown convincing evidence of ULF(Ultra Low Frequency) magnetic signatures before large earthquakes(Johnston 1997;Hayakawa et al.,2008 ).Different from those early studies,this researc... 详细信息

关键词： ULF Geomagnetic Anomalies Possibly Due to Crustal Activities in Boso and Izu Peninsula,Japan

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