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Preparation and Identification of HLA-A*1101 Tetramer Loading with Human Cytomegalovirus pp65 Antigen Peptide

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Cellular & Molecular Immunology 2007年第2期4卷 141-146页

作者： Fengyao Li Lihui Xu Qingbing zha Xiaoyun chi Qiantao Jia Kianhui He Institute of Tissue Transplantation and Immunology College of Life Science and Technology Jinan University Guangzhou 510632 China Institute of Bioengineering College of Life Science and Technology Jinan University Guangzhou 510632 China Clinical Experimental Center of the First Affiliated Hospital Jinan University Guangzhou 510632 China

MHC/peptide tetramer technology has been widely used to study antigen-specific T cells, especially for identifying virus-specific CD8^＋ T cells in humans. The tetramer molecule is composed of HLA heavy chain, β2-microglobulin （β2m）, an antigenic peptide, and fluorescent-labeled streptavidin. To further investigate the HLA-A＊1101-restricted CD8^＋ T cell responses against human cytomegalovirus （HCMV）, we established an approach to prepare HLA-A＊1101 tetramer complexed with a peptide from HCMV. The cDNA encoding HLA-A＊1101 heavy chain was cloned and the prokaryotic expression vector for the ectodomain of HLA-A＊1101 fused with a BirA substrate peptide （HLA-A＊1101-BSP） at its carboxyl terminus was constructed. The fusion protein was highly expressed as inclusion bodies under optimized conditions in Escherichla coli. Moreover, HLA-A＊1101-BSP protein was refolded in the presence of β2m and an HCMV peptide pp6516.24 （gPISgHVLK, gPI）. Soluble HLA-A＊1101-gPI monomer was biotinylated and purified to a purity of 95%, which was subsequently combined with streptavidin to form tetramers at a yield of 〉 80%. The HLA-A＊1101-gPI tetramers could bind to virus-specific CD8^＋ T cells, suggesting soluble HLA-A＊1101-gPI tetramers were biologically functional. This study provides the basis for further evaluation of HLA-A＊1101-restricted CD8^＋ T cell responses against HCMV infection.

关键词： HLA-A* 1101 tetramer cytomegalovirus CD8^+T cell epitope

High Level Expression of HLA-A ~*0203-BSP Fusion Protein in Escherichia coli and Construction of Soluble HLA-A ~*0203 Monomer and Tetramer Loaded with Epstein-Barr Virus Peptide

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Cellular & Molecular Immunology 2007年第4期4卷 301-308页

作者： Qiantao Jia Lihui Xu Qingbing zha Xiaoyun chi Fengyao Li Xianhui He Institute of Tissue Transplantation and Immunology Institute of Bioengineering College of Life Science and Technology.Jinan University 601 Huangpu Dadao West Guangzhou 510632 China

Major histocompatibility complex （MHC） tetramer technology is critical for characterization of antigen-specific T cells. In the present study we reported the successful generation of HLA-A＊0203 tetramer loaded with Epstein- Barr virus EBNA3596-604 peptide （SVRDRLARL, SVR）. Prokaryotic expression vector for the ectodomain of the heavy chain of HLA-A＊0203 fused with a BirA substrate peptide （HLA-A＊0203-BSP） was constructed and the expression conditions of the fusion protein in Escherichia coli （E. coli） were optimized. The fusion protein was highly expressed in inclusion bodies within E. coil It was then refolded in the presence of 132-microglobulin and SVR peptide to form a soluble HLA-A＊0203-SVR monomer. After biotinylation with BirA, the monomer was purified by anion-exchange chromatography and its purity was up to 95%. The tetramer was then formulated by mixing the biotinylated monomer with streptavidin-PE at a ratio of 4：1. Flow cytometry showed that this tetramer could specifically react with antigen-specific CD8^＋ T cells, indicating that it was biologically functional. These results provide a foundation for further characterization of antigen-specific CD8^＋ T cells from HLA-A＊0203 subjects.

关键词： HLA-A*0203 tetramer Epstein-Barr virus prokaryotic expression biotinylation

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三相电压源型换流器故障耐受能力分析与评估

电力系统自动化 2016年第18期40卷 109-115页

作者：彭宇黄萌查晓明 chi K.TSE 武汉大学电气工程学院湖北省武汉市430072 香港理工大学电子与资讯工程系

三相电压源型并网换流器由于含有大量电力电子开关器件及相应控制环节,是一种强非线性系统。在交直流混合系统中受到电网侧故障或直流侧负载变化等扰动影响,大容量换流器将产生复杂的响应行为,给电网带来难以预计的影响。文中考虑电网... 详细信息

三相电压源型并网换流器由于含有大量电力电子开关器件及相应控制环节,是一种强非线性系统。在交直流混合系统中受到电网侧故障或直流侧负载变化等扰动影响,大容量换流器将产生复杂的响应行为,给电网带来难以预计的影响。文中考虑电网中三相电压暂降的典型故障,基于平均模型分析了三相电压源型换流器电流环的非线性特性。在此基础上,根据李雅普诺夫稳定性判据分析了故障前后换流器的稳定性,并以故障深度和故障持续时间为指标对换流器故障耐受能力进行评估。最后,通过并网系统的逐周期仿真验证了理论分析和计算的有效性。

关键词：并网换流器非线性分析李雅普诺夫稳定性故障耐受能力

Ultra-Low Linewidth Frequency Stabilized Integrated Lasers: A New Frontier in Integrated Photonics

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ZTE Communications 2024年第4期22卷 29-39页

作者： gU Zhenqian YANg Zhen zha Lulu HU Junhui chi Nan SHEN Chao School of Information Science and Technology Fudan UniversityShanghai 200438China

With the advancement of photonic integration technology,ultra-low linewidth frequency-stabilized lasers have demonstrated significant potential in precision measurement,quantum communication,atomic clocks,*** review summarizes the latest developments in integrated photonics for achieving ultra-low linewidth lasers,particularly breakthroughs made by integrating Brillouin *** discuss the design principles,manufacturing processes,performance characteristics,and potential value of these lasers in various applications.

关键词： photonic integrated circuit(PIC) ultra-low linewidth Brillouin lasers high Q-factor

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基于剑桥模型的砂土剪胀模型

北京交通大学学报 2008年第1期32卷 49-53页

作者：迟明杰赵成刚北京交通大学土木建筑工程学院北京100044

对于中密砂和密砂,剪胀现象是一个比较显著的特征,相变线是描述剪胀现象的一条特征线,相变线比较容易确定.本文在已有弹塑性模型基础上,对硬化参数进行修正,并引入与相变线相关的状态参量,建立了一个新的剪胀性砂土本构模型.该模型尤其... 详细信息

对于中密砂和密砂,剪胀现象是一个比较显著的特征,相变线是描述剪胀现象的一条特征线,相变线比较容易确定.本文在已有弹塑性模型基础上,对硬化参数进行修正,并引入与相变线相关的状态参量,建立了一个新的剪胀性砂土本构模型.该模型尤其适合于中密砂和密砂,对于松砂则退化为原弹塑性模型.模型包含9个材料参数,参数比较容易确定.数值模拟结果能较好的描述砂土的主要特性.

关键词：岩土工程砂土本构模型硬化参数相变线状态参量

Dual ultra-wideband(UWB)radar-based sleep posture recognition system:Towards ubiquitous sleep monitoring

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Engineered Regeneration 2023年第1期4卷 36-43页

作者： Derek Ka-Hei Lai Li-Wen zha Tommy Yau-Nam Leung Andy Yiu-Chau Tam Bryan Pak-Hei So Hyo-Jung Lim Daphne Sze Ki Cheung Duo Wai-chi Wong James Chung-Wai Cheung Department of Biomedical Engineering Faculty of EngineeringThe Hong Kong Polytechnic UniversityHong Kong 999077China Department of Bioengineering Imperial CollegeLondon SW72AZUnited Kingdom Research Institute of Smart Ageing The Hong Kong Polytechnic UniversityHong KongChina School of Nursing The Hong Kong Polytechnic UniversityHong KongChina

Sleep posture monitoring is an essential assessment for obstructive sleep apnea(OSA)*** objective of this study is to develop a machine learning-based sleep posture recognition system using a dual ultra-wideband radar *** collected radiofrequency data from two radars positioned over and at the side of the bed for 16 patients performing four sleep postures(supine,left and right lateral,and prone).We proposed and evaluated deep learning approaches that streamlined feature extraction and classification,and the traditional machine learning approaches that involved different combinations of feature extractors and *** results showed that the dual radar system performed better than either single *** statistical features with random forest classifier yielded the best accuracy(0.887),which could be further improved via an ablation study(0.938).Deep learning approach using transformer yielded accuracy of 0.713.

关键词： Obstructive sleep apnea Deep learning Sleep monitoring Feature extraction Ablation study

The preparation and anti-angiogenic activity of polysaccharides from Karenia mikimotoi

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The preparation and anti-angiogenic activity of polysacchari...

相对论重离子碰撞中光子-光子相互作用的碰撞参数依赖性

中国化学会第十届全国天然有机化学学术会议

作者： Cheng-Yu Tan Liang Kong Shi-Jiao zha Xiao-Hui chi Bin Liu Marine Environmental Engineering College Dalian Ocean University

Karenia mikimotoi is a kind of bloom microalgae which can often result in serious ocean harm in the *** studies focused on its toxin from *** and its *** article was on the preparation of the polysaccharides from *** and their anti-angiogenic *** preparation process was assessed by comparing different extraction such as soxhlet’s extraction,

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物理学报 2023年第20期72卷 36-44页

作者：杨帅唐泽波杨驰查王妹华南师范大学量子物质研究院原子亚原子结构与量子调控教育部重点实验室广州510006 中国科学技术大学核探测与核电子学国家重点实验室合肥230026 山东大学前沿交叉科学青岛研究院粒子物理与粒子辐照教育部重点实验室青岛266237

在相对论重离子碰撞中,接近光速的重离子产生的超强电磁场,由于洛伦兹收缩效应可等效为线性极化的准实光子,进而诱发光子-光子相互作用产生正负轻子对.相对论重离子对撞机RHIC和大型强子对撞机LHC上的国际合作实验在非超周边重离子碰撞... 详细信息

在相对论重离子碰撞中,接近光速的重离子产生的超强电磁场,由于洛伦兹收缩效应可等效为线性极化的准实光子,进而诱发光子-光子相互作用产生正负轻子对.相对论重离子对撞机RHIC和大型强子对撞机LHC上的国际合作实验在非超周边重离子碰撞中观测到相干光致产生过程,发现正负轻子对的横动量分布相比于其在超周边碰撞发生显著的展宽,为研究解禁闭物质--夸克胶子等离子体的电磁性质提供了新途径.本文主要回顾相对论重离子碰撞中光子-光子相互作用对碰撞参数依赖的实验研究,并讨论其在侦测夸克胶子等离子体电磁性质方面的重要意义.

关键词：相对论重离子碰撞夸克胶子等离子体光子-光子相互作用前向中子标定双轻子

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融合InSAR和gA的开采沉陷预计参数反演模型研究

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武汉大学学报（信息科学版） 2018年第11期43卷 1635-1641页

作者：王磊张鲜妮池深深查剑锋安徽理工大学测绘学院安徽淮南232001 中国矿业大学国土环境与灾害监测国家测绘地理信息局重点实验室江苏徐州221116

针对基于常规合成孔径雷达差分干涉测量（differential interferometry synthetic aperture radar,D-InSAR）技术成果无法反演全部概率积分参数问题,开展了融合D-InSAR和遗传算法（genetic algorithm,gA）的概率积分参数反演方法研究。... 详细信息

针对基于常规合成孔径雷达差分干涉测量（differential interferometry synthetic aperture radar,D-InSAR）技术成果无法反演全部概率积分参数问题,开展了融合D-InSAR和遗传算法（genetic algorithm,gA）的概率积分参数反演方法研究。（1）依据采动区D-InSAR视线（line of sight,LOS）方向变形为下沉和南北、东西方向水平移动沿LOS方向投影的关系,基于gA理论,首次构建了融合D-InSAR和gA的概率积分法预计全参数反演模型,并编制了求参软件。模拟实验结果表明,q、tanβ、b、θ的相对误差不超过6.7%,拐点偏移距S相对误差不超过20%（参数敏感度低,对求参整体效果影响小）;求参拟合下沉误差为-5.90-6.10mm,拟合中误差约为±2.20mm,整体求参效果较好。（2）利用融合D-InSAR和gA的概率积分法预计全参数反演模型,在一定的假设条件下,对2012年3月11日9310工作面开采沉陷的动态概率积分参数进行了求取,得到q=0.172,b=0.13,tanβ=2.08,θ=88°,S1=-10m,S2=9m,S3=-85m,S4=30m,拟合误差约为-35.00-45.00mm,拟合中误差为±15.16mm,并认为S3=-85m主要为9310工作面开采导致邻近9312采空区边界的悬臂梁或砌体梁失稳＂活化＂所致。

关键词： D-InSAR LOS方向移动遗传算法概率积分参数