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Demarcating the membrane damage for the extraction of functional mitochondria

Microsystems & Nanoengineering 2018年第1期4卷 1-12页

作者： Md Habibur Rahman Qinru Xiao Shirui Zhao Fuyang Qu Chen Chang An-chi wei Yi-Ping Ho Department of Biomedical Engineering The Chinese University of Hong KongShatinNew TerritoriesHong Kong SARChina Shun Hing Institute of Advanced Engineering The Chinese University of Hong KongShatinNew TerritoriesHong Kong SARChina Graduate Institute of Biomedical Electronics and Bioinformatics National Taiwan University

Defective mitochondria have been linked to several critical human diseases such as neurodegenerative disorders,cancers and cardiovascular ***,the detailed characterization of mitochondria has remained relatively unexplored,largely due to the lack of effective extraction methods that may sufficiently retain the functionality of mitochondria,particularly when limited amount of sample is *** this study,we explore the possibility of modulating hydrodynamic stress through a cross-junction geometry at microscale to selectively disrupt the cellular membrane while mitochondrial membrane is *** operational conditions are empirically optimized to effectively shred the cell membranes while keeping mitochondria intact for the model mammalian cell lines,namely human embryonic kidney cells,mouse muscle cells and neuroblastoma ***,the disruption of cell membranes with higher elastic moduli(neuroblastoma)requires elevated *** study also presents a comparative analysis of total protein yield and concentrations of extracted functional mitochondria with two commercially available mitochondria extraction approaches,the Dounce Homogenizer and the Qproteome®Mitochondria Isolation Kit,in a range of cell *** findings show that the proposed“microscale cell shredder”yields at least 40%more functional mitochondria than the two other approaches and is able to preserve the morphological integrity of extracted mitochondria,particularly at low cell concentrations(5–20×10^(4) cells/mL).Characterized by its capability of rapidly processing a limited quantity of samples(200μL),demarcating the membrane damage through the proposed microscale cell shredder represents a novel strategy to extract subcellular organelles from clinical samples.

关键词： damage elevated preserve

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Separation, Purification and Identification of Antioxidant Compositions in Black Rice

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Agricultural Sciences in china 2006年第6期5卷 431-440页

作者： ZHANG Ming-wei GUO Bao-jiang ZHANG Rui-fen chi Jian-wei wei Zhen-cheng XU Zhi-hong ZHANG Yan TANG Xiao-jun Key Laboratory of Functional Food Ministry of Agriculture/Bio-Tech Research Institute Guangdong Academy of Agricultural Sciences Guangzhou 510640 P.R.China College of Life Science South China Normal University Guangzhou 510630 P.R.China

To separate, purify and identify the antioxidative compositions of black rice, using total antioxidation capacity （TAC） as an activity-monitoring parameter, different fractions of black rice antioxidative extracts were obtained using solvents of different polarities such as petroleum ether, chloroform, ethyl acetate and normal butyl alcohol. The main anfioxidative components were separated from the strongest antioxidative fractions by using Sephadex LH-20 resin and the structures were analyzed by ultraviolet-vis, infra-red, ESI-MS, ^1H-NMR and ^13C-NMR spectrums. Results showed that the water fraction and normal butyl alcohol antioxidafive extract fraction of black rice had the strongest antioxidation capacities and their TACs reached 383 and 392 ku g^-1, respectively. Four main antioxidative components were separated from the water fraction and their TACs reached 976, 878, 1 134 and 1087 ku g^-, respectively. The spectroscopy analysis indicated that the four active components of the antioxidative extract of black rice were four anthocyanin compounds of malvidin, pelargonidin- 3, 5-diglucoside, cyaniding-3-glucoside and cyaniding-3, 5-diglucoside. It is concluded that the anthocyanin compounds are the most important substantial foundations for antioxidation.

关键词： black rice antioxidative composition structure identification

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健康指数概念及理论模型

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中华疾病控制杂志 2022年第10期26卷 1118-1123,1128页

作者：迟蔚蔚山东健康医疗大数据管理中心济南250002

目的建立健康指数的理论模型及范式,构建健康指数的理论方法体系,为健康指数构建提供一般性、普适性的理论依据。方法针对健康指数构建中的瓶颈问题,在健康决定因素模型、社会生态模型、星球健康模型、人口健康模型和同一健康模型指导下... 详细信息

目的建立健康指数的理论模型及范式,构建健康指数的理论方法体系,为健康指数构建提供一般性、普适性的理论依据。方法针对健康指数构建中的瓶颈问题,在健康决定因素模型、社会生态模型、星球健康模型、人口健康模型和同一健康模型指导下,创建健康指数的理论模型及范式。结果构建了融现实世界社会生态模型和虚拟世界社会生态模型为一体的广义健康指数模型(generalized health index model,GHIM)。基于GHIM发展了包含“广义健康决定因素域、健康指数域、目标导向域、技术流程栈”的健康指数理论范式。结论所创建的健康指数理论模型及范式,为构建不同领域或层级的健康指数提供了一般性、普适性的理论模型及范式。

关键词：健康指数理论模型和范式广义健康指数模型

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面对相同供应商时市场进人决策的博弈分析

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系统工程理论与实践 2012年第6期32卷 1232-1240页

作者：叶青迟巍清华大学经济管理学院北京100084

论文研究了两个销售商在拥有各自销售市场并面对相同供应商情况下的市场进入策略.分析了单个企业进入对方市场和两个企业同时互相进入对方市场的情况,得出了在不同情况下供应商的最优定价策略,以及销售商们的采购数量和销售商们在各市... 详细信息

论文研究了两个销售商在拥有各自销售市场并面对相同供应商情况下的市场进入策略.分析了单个企业进入对方市场和两个企业同时互相进入对方市场的情况,得出了在不同情况下供应商的最优定价策略,以及销售商们的采购数量和销售商们在各市场上输出的产品数量.研究发现在大多数情况下销售商们的纳什均衡解为互相进入对方市场.但是当两个市场的市场规模和价格弹性相近的时候,销售商们面临"囚徒困境"——虽然互相进入对方的市场是市场进入博弈的纳什均衡,但销售商的利润值均低于各自选择不进入对方市场时的收益.

关键词：供应链市场进入博弈分析纳什均衡

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面向PCC决策的用户数据库构建方案研究

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移动通信 2017年第5期41卷 45-50页

作者：池炜成中国电信股份有限公司广东研究院广东广州510630

为了避免PCC决策中用户数据不一致问题,同时降低PCC业务运营复杂度,首先分析了用户数据在PCC决策过程中的作用,总结现有PCC决策用户数据库构建方案,然后探讨现有方案在运营中可能存在的问题,最后讨论了另一种面向PCC决策的用户数据库构... 详细信息

为了避免PCC决策中用户数据不一致问题,同时降低PCC业务运营复杂度,首先分析了用户数据在PCC决策过程中的作用,总结现有PCC决策用户数据库构建方案,然后探讨现有方案在运营中可能存在的问题,最后讨论了另一种面向PCC决策的用户数据库构建方案,以提高PCC决策的准确性,降低PCC业务运营难度和成本。

关键词：策略与计费控制决策用户签约存储库用户数据存储库业务支撑系统融合数据库

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A Kinase-Phosphatase-Transcription Factor Module Regulates Adventitious Root Emergence in Arabidopsis Root-Hypocotyl Junctions

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Molecular Plant 2020年第8期13卷 1162-1177页

作者： Zechen Bai Jing Zhang Xin Ning Hailong Guo Xiumei Xu Xiahe Huang Yingchun Wang Zhubing Hu Congming Lu Lixin Zhang wei chi Photosynthesis Research Center Key Laboratory of PhotobiologyInstitute of BotanyChinese Academy of SciencesNanxincun 20XiangshanBeijing 100093China University of chinese Academy of Sciences Beijing 100049China State Key Laboratory of Molecular Developmental Biology Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China State Key Laboratory of Crop Stress Adaptation and Improvement School of Life SciencesHenan University85 Minglun StreetKaifeng 475001China State Key Laboratory of Crop Biology College of Life SciencesShandong Agricultural UniversityTaianShandong 271018China The Innovative Academy of Seed Design Chinese Academy of SciencesBeijing 100101China

Adventitious roots form from non-root tissues as part of normal development or in response to stress or *** root primordia form in the source tissue,and during emergence the adventitious roots penetrate the inner cell layers and the epidermis;however,the mechanisms underlying this emergence remain largely ***,we report that a regulatory module composed of the AP2/ERF transcription factor ABSCISIC ACID INSENSITIVE 4(ABI4),the MAP kinases MPK3 and MPK6,and the phosphatase PP2C12 plays an important role in the emergence of junction adventitious roots(J-ARs)from the root-hypocotyl junctions in Arabidopsis ***4 negatively regulates J-AR emergence,preventing the accumulation of reactive oxygen species and death of epidermal cells,which would otherwise facilitate J-AR *** by MPK3/MPK6 activates ABI4 and dephosphorylation by PP2C12 inactivates ***3/MPK6 also directly phosphorylate and inactivate PP2C12 during J-AR *** propose that this"double-check"mechanism increases the robustness of MAP kinase signaling and finely regulates the local programmed cell death required for J-AR emergence.

关键词： ABI4 MPK3/MPK6 PP2C12 adventitious root root-hypocotyl junction PCD

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Biomechanical finite element analysis of typical tibiotalar arthrodesis

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Medicine in Novel Technology and Devices 2021年第3期11卷 226-235页

作者： Fangbo Bing chi wei Pan Liu Zhenzi Li Yu Deng Zuoqi Zhang Shaobo Zhu School of Civil Engineering Wuhan UniversityWuhanHubei430072PR China Zhongnan Hospital of Wuhan University WuhanHubei430071PR China

Tibiotalar arthrodesis(TTA)is a common surgical method for post-traumatic arthrosis and primary osteoarthrosis of *** screw fixation exhibits such advantages of higher fusion rate and fewer complications that it has become the mainstream surgical procedure in ***,there are still disputes over the choice of screw type,number and nailing *** this study,a 3D model of tibiotalar joint was established based on CT *** representative types of screws were modeled according to their real *** was done in Hypermesh 2017,and the finite element simulations of different surgical schemes and postoperative ankle joint movements were performed via Abaqus *** stress distribution in tibiotalar joints and screws,interarticular displacement,and screw deformation were extracted for comparative *** results show that:(1)the three-screw fixation scheme with smaller displacements and higher contact pressure is superior to the two-screw one;(2)the relative displacements of the tibia and talus of full-thread screw under all loads were reduced by approximately 50%compared with those of lag screw,showing better stability;(3)the 60scheme is more favorable for joint fusion and more stable under vertical load,while the 75has the best bending resistance;(4)the stress and strain distributions in screws indicate that the screw break is most likely to happen near its contact region with the bone inlet,the maximum stress reaches 212.2 *** study is of great significance for the selection and optimization of the surgical plan.

关键词： Surgical design and optimization Screw type and number Stability Stress and strain analyses Finite element method

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Antioxidations and Their Correlations with Total Flavones and Anthocyanin Contents in Different Black Rice Varieties

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Agricultural Sciences in china 2005年第11期4卷 811-819页

作者： ZHANG Ming-wei GUO Bao-jiang chi Jian-wei wei Zhen-cheng XU Zhi-hong ZHANG Yan ZHANG Rui-fen Key Laboratory of Functional Food Ministry of Agriculture/Bio-Tech Research Institute Guangdong Academy of Agricultural Sciences Guangzhou 510640 P.R.China College of Life Science Huanan Normal University Guangzhou 510630 P.R.China

The antioxidations and their correlations with total flavones and anthocyanin contents in different black rice varieties were studied. The results indicated that the great differences in total antioxidant capacity （TAC）, scavenging free radical capacity （SFRC）, total flavones and anthocyanin contents existed among 242 black rice varieties. Comparisons of TAC, SFRC, total flavones and anthocyanin contents of different black rice types showed that the differences between black rice and white rice, indica one and japonica one, and nonglutinous one and glutinous one were significant at 0.01 levels, respectively. This showed that the TAC, SFRC, total flavones and anthocyanin contents of japonica type were higher than those of indica one, and nonglutinous type were higher than glutinous one. According to the fast clustering procedure, 242 black rice varieties could be clustered into 10 clusters, 184 indica rice varieties into 10 clusters, and 58 japonica rice varieties into 6 clusters. Most significant （P〈0.01） correlations existed between TAC and total flavones and anthocyanin contents, and between SFRC and total flavones and anthocyanin contents of black rice, respectively. This showed that the antioxidation of black rice was closely correlated with its active compositions of flavones and anthocyanin.

关键词： Black rice Antioxidation Total flavones content Anthocyanin content Correlation

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The DELLA-ABI4-HY5 module integrates light and gibberellin signals to regulate hypocotyl elongation

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Plant Communications 2023年第5期4卷 223-233页

作者： Haibo Xiong Dandan Lu Zhiyuan Li Jianghao Wu Xin Ning weijun Lin Zechen Bai Canhui Zheng Yang Sun wei chi Lixin Zhang Xiumei Xu State Key Laboratory of Crop Stress Adaptation and Improvement School of Life SciencesHenan UniversityJinming AvenueKaifeng 475004China Sanya Institute of Henan University Sanya 572025China Photosynthesis Research Center Key Laboratory of PhotobiologyInstitute of BotanyChinese Academy of SciencesNanxincun 20XiangshanBeijing 100093China Shenzhen Institute of Advanced Technology Chinese Academy of SciencesShenzhen 518055China

Plant growth is coordinately controlled by various environmental and hormonal signals,of which light and gibberellin(GA)signals are two critical factors with opposite effects on hypocotyl *** interactions between the light and GA signaling pathways have been studied extensively,the detailed regulatory mechanism of their direct crosstalk in hypocotyl elongation remains to be fully ***,we reported that ABA INSENSITIVE 4(ABI4)controls hypocotyl elongation through its regulation of cellelongation-related genes,but whether it is also involved in GA signaling to promote hypocotyl elongation is *** this study,we showthat promotion of hypocotyl elongation by GA is dependent on ABI4 *** interact directly with ABI4 and inhibit its DNA-binding *** turn,ABI4 combined with ELONGATED HYPOCOTYL 5(HY5),a key positive factor in light signaling,feedback regulates the expression of the GA2ox GA catabolism genes and thus modulates GA *** together,our results suggest that the DELLA-ABI4-HY5 module may serve as a molecular link that integrates GA and light signals to control hypocotyl elongation.

关键词： light signal GA signal hypocotyl elongation DELLA ABI4 HY5

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A deadly needle-tract:Inward exsanguination

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World Journal of Emergency Medicine 2022年第1期13卷 74-74页

作者： chin-shen Lin chi-wei Lee Department of Emergency Medicine Kaohsiung Medical University HospitalKaohsiung 807TaiwanChina Research Center for Environmental Medicine Kaohsiung Medical UniversityKaohsiung 807TaiwanChina

Dear editor,A 41-year-old male with liver cirrhosis suff ered from abdominal fullness due to massive ascites.A 16-gauge needle catheter was inserted by Z-tract technique[1]into the right lower quadrant,and 3,000 mL of strawcolored ascites was drained.A pressure bandage was *** was no evidence of *** patient tolerated the procedure well and was discharged stable(blood pressure 124/72 mmHg[1 mmHg=0.133 kPa];pulse rate 83 beats/minute).

关键词： needle ascites bleeding

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