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Vesicle-bound and free microRNAs in spent cell culture medium and biological fluids

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Advances in Bioscience and Biotechnology 2012年第8期3卷 1091-1095页

作者： Gang Wang Ka-Bik Lai Bonnie Ching-Ha Kwan Kai-Ming Chow Philip Kam-Tao Li cheuk-chun szeto Department of Medicine & Therapeutics The Chinese University of Hong Kong Hong Kong China

Background: In addition to the control of gene transla- tion intra-cellularly, microRNAs (miRNAs) have been found to exist extracellularly. However, extracellular miRNA identified in previous studies were largely confined to microvesicles. It remains uncertain whether free extracellular miRNA exists. Methods: We quantify a panel of miRNAs (miRNA 200 family, miR-205, and miR-192) in spent culture medium of renal tubular epithelial cells, as well as serum and urine from patients with systemic lupus erythematosus and healthy controls. Microvesicle bound and free miRNA were separated by ultracentrifugation. Results: In spent cell culture medium, we found substantial amount of miR-200a, miR-200b, miR-200c, miR-141, miR-429, miR-205, and miR-192 in microvesicles, as well as the presence of miR-200a, miR-200b, miR-429, miR-205 and miR-192, but not miR-200c or miR-141 as non-vesicle bound free form. In healthy individuals, we found substantial amount of miR-200a, miR-200b, miR-200c, miR-141 and miR-192 in microvesicles from serum and urine, while only miR-141 exists as free form in both serum and urine. There was no significant difference in serum or urinary free miR-141 levels between SLE patients and healthy controls. In SLE patients, urinary free miR-141 level was signifiantly higher than serum. Neither serum nor urinary free miR-141 levels correlated with lupus disease activity. Conclusion: We found miRNA is present extracellularly, both within microvesicles and as free form, in spent cell culture medium, serum and urine. The biological role of extracellular miRNA requires further study.

关键词： Cell Signaling Fibrosis Cytokine

Intra-renal and urinary RNA expression of podcyte-associated molecules in patients with IgA nephropathy

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Advances in Bioscience and Biotechnology 2010年第3期1卷 232-237页

作者： Gang Wang Bonnie Ching Kwan Fernand Mac-Moune Lai Paul Cheung-Lung Choi Kai-Ming Chow Philip Kam-Tao Li cheuk-chun szeto 不详

Background Podocyte injury probably plays important roles in the pathogenesis of IgA nephropathy (IgAN). We studied intra-renal and urinary messenger RNA (mRNA) expression of podocyte-associated molecules in patients with IgAN. Methods We studied 43 consecutive patients with biopsy-proven IgAN. Intra-renal and urinary expression of mRNAs was determined and compared to that of 20 patients with nephrectomy for kidney cancer and 12 normal subjects. Results Intra-renal mRNA expression levels of nephrin, podocin and synaptopodin were significantly lower in patients with IgAN than that of controls. In contrast, their urinary mRNA expression levels were similar. Intra-renal gene expression of nephrin inversely correlated with proteinuria (r = –0.620, P <0.001), GFR (r = 0.538, P <0.001), and the degree of tubulointerstitial scarring (r = –0.423, P = 0.013). After followed for an average of 33.4 ? 12.6 months, intra-renal nephrin expression significantly correlated with the rate of GFR decline (r = 0.324, P = 0.041). Conclusions Intra-renal mRNA expression of podocyte associated molecules was down-regulated in patients with IgAN, and the degree of down- regulation of nephrin correlated with disease severity and the rate of progression. Our result supports the hypothesis that podocyte injury is an important component in the pathophysiology of IgAN.

关键词： Glomerulonephritis Proteinuria Chronic Kidney Diseases

The gene expression of NGAL and TLR9 in glomerulus and tubulo-interstitium of patients with lupus nephritis

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Advances in Bioscience and Biotechnology 2011年第1期2卷 33-39页

作者： Jianxin Lu Bonnie Ching-Ha Kwan Fernand Mac-Moune Lai Paul Cheung-Lung Choi Lai-Shan Tam Edmund Kwok-Ming Li Kai-Ming Chow Gang Wang Philip Kam-Tao Li cheuk-chun szeto 不详

Background The role of neutrophil gelatinase associated lipocalin (NGAL) and Toll-like receptor 9 (TLR9) in the pathogenesis of lupus nephritis remain elusive. Methods We quantified the glomerular and tubulointerstitial mRNA expression of NGAL and TLR9 in 42 patients with lupus nephritis (LN group) and 10 controls. Results As compared to controls, LN group had higher glomerular expression of TLR9, and higher tubulointerstitial expression of NGAL and TLR9. Tubulointerstitial NGAL expression significantly correlated with proteinuria (r = 0.492;p = 0.003), renal function (r = -0.386;p = 0.022) and histological chronicity index (r = 0.540;p = 0.004). Proteinuria had significant correlation with glomerular (r = 0.554;p = 0.001) and tubulointerstitial (r = 0.379;p = 0.043) TLR9 expression. Furthermore, there was a significant difference in tubulointerstitial expression of NGAL between treatment response groups. Conclusion There is an increase in intra-renal mRNA expression of NGAL and TLR9 in LN. Although tubulointerstitial expression of NGAL does not correlate with systemic disease activity, it correlates with proteinuria, renal function, and therapeutic response. The role of NGAL in the pathogensis in LN, as well as its application as biomarker for lupus nephritis, requires further study.

关键词： Lupus Nephritis Immunology Cytokine

Fast internal preheating of 4680 lithium-ion batteries in cold environments

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Nano Research 2024年第10期17卷 8794-8802页

作者： Chuyue Guan Harrison szeto Olivia Wander Vijay Kumar Raphaele JClément Yangying Zhu Department of Mechanical Engineering University of California Santa BarbaraSanta BarbaraCA 93106USA Department of Chemistry and Biochemistry University of California Santa BarbaraSanta BarbaraCA 93106USA Department of Materials University of California Santa BarbaraSanta BarbaraCA 93106USA

Lithium-ion batteries are expected to operate within a narrow temperature window around room temperature for optimal performance and ***,in cold environments,electric vehicle battery packs must be extensively preheated prior to charge or ***,conventional preheating is accomplished externally,which is slow and thus significantly increases charging ***,internal heating has been demonstrated as a potential solution to quickly and uniformly preheat a lithium-ion pouch ***,internal heating has not been evaluated in other battery formats such as cylindrical *** this work,we present a numerical model of a 4680 battery with internal heaters for fast preheating in cold *** effects that the number of heater layers,heating duration,resting duration,environmental temperature,and boundary heat transfer coefficient have on the temperature heterogeneity of the battery were *** results show that internal heating alone reduces the temperature variation within the battery by a factor of 5 compared to external heating,and by a factor of 20 when combining internal and external *** study further proves that internal preheating of lithium-ion batteries is a promising thermal management strategy,and provides guidance on potential design considerations and heating protocols to implement internal heating.

关键词： lithium-ion battery 4680 battery thermal management internal battery preheating COMSOL Multiphysics thermal simulation battery temperature heterogeneity

Vesicle-bound and free microRNAs in spent cell culture medium and biological fluids

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The gene expression of NGAL and TLR9 in glomerulus and tubulo-interstitium of patients with lupus nephritis

Advances in Bioscience and Biotechnology（生命科学与技术进展） 2012年第8期

作者： Gang Wang Ka-Bik Lai Bonnie Ching-Ha Kwan Kai-Ming Chow Philip Kam-Tao Li cheuk-chun szeto

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Theory of Critical Phenomena with Memory

Advances in Bioscience and Biotechnology（生命科学与技术进展） 2011年第1期

作者： Jianxin Lu Bonnie Ching-Ha Kwan Fernand Mac-Moune Lai Paul Cheung-Lung Choi Lai-Shan Tam Edmund Kwok-Ming Li Kai-Ming Chow Gang Wang Philip Kam-Tao Li cheuk-chun szeto Department of Medicine & Therapeutics and Department of Anatomical & Cellular Pathology Prince of Wales Hospital The Chinese University of Hong Kong Shatin Department of Nephrology Shanghai Ninth People’s Hospital Shanghai Jiao Tong University School of Medicine Division of Nephrology Renmin Hospital of Wuhan University

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Chinese Physics Letters 2022年第12期39卷 1-7页

作者： Shaolong Zeng Sue Ping szeto Fan Zhong State Key Laboratory of Optoelectronic Materials and Technologies School of PhysicsSun Yat-Sen UniversityGuangzhou 510275China

Memory is a ubiquitous characteristic of complex systems,and critical phenomena are one of the most intriguing phenomena in ***,we propose an Ising model with memory,develop a corresponding theory of critical phenomena with memory for complex systems,and discover a series of surprising novel *** show that a naive theory of a usual Hamiltonian with a direct inclusion of a power-law decaying long-range temporal interaction violates radically a hyperscaling law for all spatial dimensions even at and below the upper critical *** entails both indispensable consideration of the Hamiltonian for dynamics,rather than the usual practice of just focusing on the corresponding dynamic Lagrangian alone,and transformations that result in a correct theory in which space and time are inextricably interwoven,leading to an effective spatial dimension that repairs the hyperscaling *** theory gives rise to a set of novel mean-field critical exponents,which are different from the usual Landau ones,as well as new universality *** exponents are verified by numerical simulations of the Ising model with memory in two and three spatial dimensions.

关键词： critical Hamiltonian scaling

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彭锐基于五脏相关理论辨治高血压病经验介绍

新中医 2024年第15期56卷 228-232页

作者：司徒嘉雯彭锐广州中医药大学第一临床医学院广东广州510405 广州中医药大学第一附属医院广东广州510405 广州中医药大学顺德医院广东佛山528300

高血压病是常见的临床慢性疾病,容易发生心、脑、肾等多器官损伤,影响患者健康。彭锐教授以国医大师邓铁涛教授“五脏相关”理论为指导,结合多年临床经验将高血压诊疗思路概括为其本在肾,其治在肝,其害在心,其复在脾,其进在肺。总结彭... 详细信息

高血压病是常见的临床慢性疾病,容易发生心、脑、肾等多器官损伤,影响患者健康。彭锐教授以国医大师邓铁涛教授“五脏相关”理论为指导,结合多年临床经验将高血压诊疗思路概括为其本在肾,其治在肝,其害在心,其复在脾,其进在肺。总结彭教授基于五脏相关理论辨治高血压病的经验,为中医药治疗高血压病提供更多治疗思路。

关键词：高血压病五脏相关邓铁涛彭锐

Influence of Strong Electron-Electron Interaction on the Peierls Transition

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Chinese Physics Letters 1997年第1期14卷 63-66页

作者： HU Lian K.Y.szeto SUN Xin Department of Physics South China Normal UniversityGuangzhou 510631 Department of Physics The Hong Kong University of Science and TechnologyHong Kong Department of Physics Fudan UniversityShanghai 200433

The influence of short-ranged Coulomb repulsion of strongly correlated electrons on the Peierls transition for a quasi-one-dimensional system is *** shows that the influence varies with the band filling and proves that the condition of single-occupation leads to a singularity on the density of states at half-filling which enhances *** also shows that dimerization is suppressed by the increase of U in the region of large-U and proves that increasing nearest-neighbor interaction V will suppress the Peierls transition temperature at half-filling,and strongly enhance it for band-filling at n≈1/4,which means the enhancement of tetramer-ization for a quarter-filled band.

关键词： Electron Peierls singularity