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Effects of Jiazhu decoction in combination with cyclophosphamide on breast cancer in mice

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Journal of Traditional Chinese Medicine 2019年第5期39卷 642-648页

作者： Guan Huiting Xie Su Liu Shangyi Xie Qing hou Shengkai Liu Huarong Zhang Yunjie Hu Yaqing Zhang chenyu Academy of Clinical Medicine Guizhou Medical UniversityGuiyang 550004China Department of Chinese Medicine the Affiliated Hospital of Guizhou Medical UniversityGuiyang 550004China the First Affiliated Hospital of Guiyang University of Chinese Medicine Guiyang 550004 China Department of Chinese Medicine the Maternity and Child Health Care of ZaozhuangZaozhuang 277100China Academy of Clinical Medicine Guizhou Medical UniversityGuiyang 550004China

OBJECTIVE: To investigate the therapeutic effects of Jiazhu decoction (JZD) in combination with cyclophosphamide (CTX) on the growth of breast cancer in mice and to explore the possible molecular mechanisms of action. METHODS: BALB/c mice were randomly divided into four groups of 10 (untreated model group, JZD group, CTX group, and JZD + CTX group) and subcutaneously injected with 4T1 mouse breast cancer cells. Tumors were allowed to establish for ~7 d before initiation of treatment with CTX (100 mg/kg every week by intraperitoneal injection) and/or JZD (0.015 mL of 1.65 g/mL crude drug, administered daily by gavage). The model group received equivalent volumes of vehicle on the same schedules. Tumor volumes were measured every 3 d. Mice were sacrificed after 3 weeks of treatment, and tumors were excised and subjected to RT-qPCR and western blot analysis to evaluate expression of the Wnt/β-catenin signaling pathway components β-catenin, c-Myc, and cyclin D1 at the mRNA and protein levels. RESULTS: The mean tumor volume was smaller and the growth rate was slower in the CTX and JZD + CTX groups compared with the model group (P < 0.05), and in the JZD + CTX group compared with the CTX and JZD groups (P < 0.05). Tumor growth was inhibited by 35.4% and 48.1% by CTX and JZD + CTX treatment, respectively (P < 0.001). The expression of β-catenin, c-Myc, and cyclin D1 mRNA and protein in tumors was significantly lower in mice treated with JZD or JZD + CTX compared with the untreated mice (P < 0.05), and was significantly lower in mice treated with JZD + CTX compared with either JZD or CTX alone (P < 0.05). CONCLUSION: JZD inhibited the growth of mouse breast cancer cells in vivo, possibly by reducing the expression of β-catenin, c-Myc, and cyclin D1. Combination therapy with JZD plus CTX had a more potent inhibitory effect on breast cancer growth compared with either agent alone.

关键词： Breast neoplasms Cyclophosphamide Wnt signaling pathway Jiazhu decoction

Effects of fermented sweet potato residue on nutrient digestibility, meat quality, and intestinal microbes in broilers

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Animal Nutrition 2024年第2期17卷 75-86页

作者： Ting Yao chenyu Wang Lifen Liang Xuan Xiang Hui Zhou Wentao Zhou Ruoxin hou Tianli Wang Liuqin He Shiyu Bin Yulong Yin Tiejun Li Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process CAS Key Laboratory of Agro-Ecological Processes in Subtropical RegionNational Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry ProductionInstitute of Subtropical AgricultureChinese Academy of Sciences University of Chinese Academy of Sciences Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation Laboratory of Animal Nutrition and Human HealthCollege of Life SciencesHunan Normal University Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection Ministry of EducationCollege of Life ScienceGuangxi Normal University

This study aimed to investigate the effects of different proportions of dietary fermented sweet potato residue(FSPR) supplementation as a substitute for corn on the nutrient digestibility, meat quality, and intestinal microbes of yellow-feathered broilers. Experiment 1(force-feeding) evaluated the nutrient composition and digestibility of mixtures with different proportions of sweet potato residue(70%, 80%,90%, and 100%) before and after fermentation. In Experiment 2(metabolic growth), a total of 420 oneday-old yellow-feathered broilers were randomly allocated to 4 groups and fed corn-soybean mealbased diets with 0, 5%, 8%, and 10% FSPR as a substitute for corn. The force-feeding and metabolic growth experiments were performed for 9 and 70 d, respectively. The treatment of 70% sweet potato residue(after fermentation) had the highest levels of crude protein, ether extract, and crude fiber and improved the digestibility of crude protein and amino acids(P hough dietary FSPR supplementation at different levels had no significant effect on growth performance and intestinal morphology, it improved slaughter rate, half-chamber rate, full clearance rate, and meat color, as well as reduced cooking loss in the breast and thigh muscles(P < 0.05). Dietary supplementation with 8% and 10% FSPR increased the serum immunoglobulin M and immunoglobulin G levels in broilers(P < 0.05). Furthermore, 10% FSPR increased the Shannon index and Ruminococcaceae＿UCG-014, Ruminococcaceae＿UCG-010 and Romboutsia abundances and decreased Sutterella and Megamonas abundances(P < 0.05). Spearman's correlation analysis showed that meat color was positively correlated with Ruminococcaceae＿UCG-014(P < 0.05) and negatively correlated with Megamonas(P < 0.05). Collectively, 70% sweet potato residue(after fermentation) had the best nutritional value and nutrient digestibility. Dietary supplementation with 8% to 10%FSPR as a substitute for corn can improve the slaughter performance, meat quality

关键词： Fermented sweet potato residue Meat quality Intestinal microbiota Broiler

Factors in?uencing the somatic cell nuclear transfer ef?ciency in pigs

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Frontiers of Agricultural Science and Engineering 2019年第1期6卷 73-80页

作者： Yong JIN Manling ZHANG Xinrong JU Shuang LIANG Qiang XIONG Lihua ZHAO Xiaowei NIE Daorong hou Qiang LIU Junzheng WANG chenyu WANG Xiaokang LI Lining ZHANG Xiaorui LIU Ying WANG Haiyuan YANG Yifan DAI Rongfeng LI Jiangsu Key Laboratory of Xenotransplantation/Key Laboratory of Targeted Intervention of Cardiovascular Disease/Collaborative Innovation Center for Cardiovascular Disease Translational Medicine Nanjing Medical University

Using a data set from our laboratory, we assessed the effects of several factors on pig cloning ef?ciency. The results demonstrated that cells at high con?uence( > 90%) used as donor cell resulted in higher pregnancy rate, delivery rate and overall cloning ef?ciency(number of live offspring born per reconstructed embryo transferred to recipients) compared with the cells at 60% to79% con?uence and 80% to 89% con?uence. Cells with four, ?ve and six passages compromised the pregnancy and delivery rates compared with ?rst passage cells. The number of blastocysts transferred by somatic cell nuclear transfer(SCNT) did not signi?cantly affect the cloning ef?ciency, but transfer of blastocyst derived from in vitro culture 5 d after SCNT achieved a signi?cantly higher pregnancy rate compared with one to two cell SCNT embryos from overnight culture. The highest pregnancy rate, delivery rate and the largest litter size were obtained when Bama Miniature pig ?broblasts were used as donor cells and Landrace/Yorkshire hybrid gilts were used as recipients. Recipients treated with chemicals for estrus synchronization had higher pregnancy rates compared with untreated recipients. Our data might be helpful for improving SCNT ef?ciency in pigs.

关键词： blastocyst donor cell estrus synchronization pregnancy rate pig cloning somatic cell nuclear transfer

金属-有机笼的合成及其在色谱分离和选择性吸附中的应用研究进展

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北京理工大学学报 2021年第5期41卷 522-530页

作者：罗爱芹孙琛瑜汤波侯慧鹏孙立权北京理工大学生命学院北京100081

金属有机笼(metal organic cages,MOCs)作为新型多孔材料的一种,具有几何形状可调、空腔结构多样、易于修饰等特点,目前在传感、催化、分子识别等领域有着较多的应用,其合成方法途径也是多种多样,同时基于MOCs的选择性吸附和色谱分离应... 详细信息

金属有机笼(metal organic cages,MOCs)作为新型多孔材料的一种,具有几何形状可调、空腔结构多样、易于修饰等特点,目前在传感、催化、分子识别等领域有着较多的应用,其合成方法途径也是多种多样,同时基于MOCs的选择性吸附和色谱分离应用也引起了研究者的极大兴趣.本文总结了近几年MOCs的合成方法与在选择性吸附和色谱分离中的应用研究进展,并对其发展前景进行了展望.

关键词：金属有机笼有机合成选择性吸附色谱分离固定相

银行资本工具的市场约束作用提升了吗?——基于或有可转债发行的实证证据

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管理现代化 2019年第4期39卷 1-4页

作者：侯鑫谢梦陕晨煜复旦大学管理学院上海200433 广州银行金融研究院广东广州510627 上海财经大学金融学院上海200433

采用2010年到2017年全球或有可转债为样本,研究银行新资本工具或有可转债的市场约束有效性。研究发现,银行破产风险、风险承担动机以及股价崩盘风险”均会显著的影响或有可转债发行利差;这一结果仅在2016年风险事件爆发后才显现,2016年... 详细信息

采用2010年到2017年全球或有可转债为样本,研究银行新资本工具或有可转债的市场约束有效性。研究发现,银行破产风险、风险承担动机以及股价崩盘风险”均会显著的影响或有可转债发行利差;这一结果仅在2016年风险事件爆发后才显现,2016年以前,政府救助预期仍然是投资者主要关注的因素。研究表明,银行债权人对于银行的市场约束作用正逐步加强。

关键词：银行资本工具或有可转债市场约束

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肉制品中鸵鸟源性成分的实时荧光PCR检测

中国食品学报 2016年第7期16卷 246-251页

作者：齐春萌杨昕霆薛晨玉赵琳娜侯彩云中国农业大学食品科学与营养工程学院北京100083 北京市食品安全监控与风险评估中心北京100041

通过实时荧光PCR技术检测肉制品中鸵鸟源性成分。根据鸵鸟线粒体细胞色素C氧化酶亚基Ⅰ基因(COX1)序列,用Primer Express和DNAMAN软件设计特异性的引物和Taq Man探针,并用阳性样本进行验证。结果表明,引物和探针对鸵鸟源性成分的特异性... 详细信息

通过实时荧光PCR技术检测肉制品中鸵鸟源性成分。根据鸵鸟线粒体细胞色素C氧化酶亚基Ⅰ基因(COX1)序列,用Primer Express和DNAMAN软件设计特异性的引物和Taq Man探针,并用阳性样本进行验证。结果表明,引物和探针对鸵鸟源性成分的特异性良好,与常见物种DNA无非目标扩增,该方法的检测灵敏度达0.27 pg/反应,适用于鸵鸟源性成分的鉴定,从而建立了肉制品中鸵鸟源性成分的实时荧光PCR鉴定方法。

关键词：实时荧光聚合酶链式反应鸵鸟源性成分线粒体COX1基因

含联苯聚芳醚砜醚酮酮无规共聚物的合成与表征

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高分子材料科学与工程 2021年第9期37卷 39-44页

作者：刘勇军周宸羽潘阳刘晓玲盛寿日侯豪情江西师范大学化学化工学院江西南昌330022

以新合成的含联苯芳醚单体4,4’-二(4-联苯氧基)二苯砜(BBPOPS)与4,4’-二苯氧基二苯砜(DPODPS)、对苯二甲酰氯(TPC)为单体,以路易斯酸无水三氯化铝(AlCl_(3))为催化剂进行三元共缩聚,制备了大分子主链含联苯结构的聚芳醚砜醚酮酮(PESE... 详细信息

以新合成的含联苯芳醚单体4,4’-二(4-联苯氧基)二苯砜(BBPOPS)与4,4’-二苯氧基二苯砜(DPODPS)、对苯二甲酰氯(TPC)为单体,以路易斯酸无水三氯化铝(AlCl_(3))为催化剂进行三元共缩聚,制备了大分子主链含联苯结构的聚芳醚砜醚酮酮(PESEKK)无规共聚物。通过核磁共振仪、红外光谱仪、差示扫描量热仪、广角X射线衍射和紫外-可见分光光度计等分析方法表征了PESEKK无规共聚物的结构与性能。实验结果表明,在大分子主链引入联苯结构能提高PESEKK无规共聚物的耐热性,玻璃化转变温度(T_(g))高于189℃,且T_(g)随着共聚物中联苯结构含量的增加而升高;PESEKK无规共聚物为非晶态结构,其热分解温度(T_(d))为544℃,具有优异的热性能。无规共聚物可溶解于二甲基亚砜、N-甲基吡咯烷酮、氯仿等有机溶剂,并可涂膜制得柔韧性薄膜,薄膜拉伸强度大于84 MPa,弹性模量大于1.89GPa,力学性能较好。

关键词：聚芳醚砜醚酮酮无规共聚物联苯

基于荷载均衡的雄安新区城市水资源协同动态配置

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给水排水 2021年第12期47卷 72-79页

作者：王晨雨侯保灯王欣何凡侯效灵周毓彦鲁帆中国水利水电科学研究院流域水循环模拟与调控国家重点实验室北京100038 华北水利水电大学水利学院郑州450046

随着经济社会的快速发展,多数地区水资源已呈现"临界"或"超载"状态。开展区域水资源配置可以有效解决此类问题,其中水资源承载能力和水资源需求合理性分析是水资源配置的重要基础,在此基础上提出相应配置手段,如"强载"或"控荷"措施以应... 详细信息

随着经济社会的快速发展,多数地区水资源已呈现"临界"或"超载"状态。开展区域水资源配置可以有效解决此类问题,其中水资源承载能力和水资源需求合理性分析是水资源配置的重要基础,在此基础上提出相应配置手段,如"强载"或"控荷"措施以应对水资源荷载不均衡问题。基于荷载均衡和协同发展理论,按照多水源优化利用与联合调配的原则,以荷载均衡为目标,动态调控,多次配置,最终实现雄安新区城市水资源优化配置。研究成果可为保障雄安新区城市水资源安全,实现区域经济、社会、生态协同可持续发展提供技术支撑。

关键词：雄安新区荷载均衡协同配置水资源承载力

基于变权重和组合隶属度函数的GIS气体绝缘状态评估

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高压电器 2021年第6期57卷 102-106,114页

作者：刘长银宋人杰赵萌赵超平侯艳权赵晨宇侯艳强国网黑龙江省电力有限公司七台河供电公司黑龙江七台河154600 东北电力大学信息工程学院吉林吉林132012 国网浙江省电力有限公司宁波供电公司浙江宁波315000 国网河北省电力公司邯郸市永年区供电分公司河北邯郸057150

在GIS气体绝缘状态评估过程中,不同的权重确定和隶属度函数选择方法会导致评价结果的不同,进而影响评价结果的准确性。为了避免主观因素的影响,文中在利用熵权法确定权重的基础上,根据变权综合理论引入均衡函数来调整各评价指标的权重... 详细信息

在GIS气体绝缘状态评估过程中,不同的权重确定和隶属度函数选择方法会导致评价结果的不同,进而影响评价结果的准确性。为了避免主观因素的影响,文中在利用熵权法确定权重的基础上,根据变权综合理论引入均衡函数来调整各评价指标的权重。此外,采用梯形、Γ形以及k次抛物线型相结合来确定各指标的隶属度函数,以避免单一隶属度函数所造成的评价误差。最后,利用综合诊断模型来确定GIS气体的绝缘状态,并对比常权重和单一隶属度评价结果。实例分析表明,该方法可以准确有效的评估GIS设备气体的绝缘状态。

关键词： GIS 熵权法权重模糊隶属度函数模糊综合评价