检索结果-南通市图书馆

M2型巨噬细胞衍生外泌体促进小胶质细胞M2型极化

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中国组织工程研究 2025年第25期29卷 5320-5327页

作者：方俊魏伟薛亚婷崔臣龙卫嘉晟石筱杨丽娟杨保仲山西医科大学山西省太原市030032 太原市中心医院山西省太原市030009

背景:目前对于M2型巨噬细胞衍生外泌体的研究多集中于促进伤口愈合及成骨细胞的增殖和分化,而很少有研究关注其对小胶质细胞表型的调控作用。目的:探讨M2型巨噬细胞衍生外泌体对于小胶质细胞的表型调控作用及分子机制。方法:①提取骨髓... 详细信息

背景:目前对于M2型巨噬细胞衍生外泌体的研究多集中于促进伤口愈合及成骨细胞的增殖和分化,而很少有研究关注其对小胶质细胞表型的调控作用。目的:探讨M2型巨噬细胞衍生外泌体对于小胶质细胞的表型调控作用及分子机制。方法:①提取骨髓原代巨噬细胞,用50 ng/m L白细胞介素4刺激巨噬细胞24 h促进巨噬细胞M2型极化,流式细胞术和细胞免疫荧光鉴定M2型巨噬细胞标志物CD206;②提取和鉴定M2型巨噬细胞衍生外泌体;③将小胶质细胞BV2随机分为3组:对照组、脂多糖组、治疗组,对照组不做处理,脂多糖组加入500 ng/m L脂多糖干预24 h,治疗组同时加入500 ng/m L脂多糖和25μg/m L M2型巨噬细胞衍生外泌体干预24 h,ELISA检测培养上清中肿瘤坏死因子α和白细胞介素10的分泌量,q RT-PCR检测细胞中诱导型一氧化氮合酶、精氨酸酶1、白细胞介素1β和白细胞介素10的m RNA表达,Western blot检测诱导型一氧化氮合酶、精氨酸酶1的蛋白表达以及核因子κB信号通路相关蛋白表达。结果与结论:①ELISA结果显示,与对照组相比,脂多糖组肿瘤坏死因子α分泌明显增多;与脂多糖组相比,治疗组肿瘤坏死因子α的分泌减少而白细胞介素10的分泌增多;②q RT-PCR结果显示,与对照组相比,脂多糖组白细胞介素1β、诱导型一氧化氮合酶m RNA表达升高;与脂多糖组相比,治疗组白细胞介素1β、诱导型一氧化氮合酶m RNA表达降低,白细胞介素10、精氨酸酶1 m RNA表达升高;③Western blot结果显示,与对照组相比,脂多糖组诱导型一氧化氮合酶蛋白表达升高;与脂多糖组相比,治疗组诱导型一氧化氮合酶蛋白表达降低,而精氨酸酶1蛋白表达升高;(4)与对照组相比,脂多糖组核因子κB信号通路中P65、p-IκB-α蛋白表达降低;与脂多糖组相比,治疗组P65、p-IκB-α蛋白表达升高。结果表明:M2型巨噬细胞衍生外泌体可以显著抑制脂多糖诱导小胶质细胞的炎症反应,促进抗炎因子白细胞介素10的表达,抑制促炎因子肿瘤坏死因子α、白细胞介素1β的表达,促进小胶质细胞表型由M1型向M2型极化,其机制可能与M2型巨噬细胞衍生外泌体抑制核因子κB信号通路激活有关。

关键词： M2巨噬细胞外泌体小胶质细胞炎症 NF-κB信号通路神经病理性疼痛极化

Dynamic Event-Triggered MPC Enhanced with Time-Varying Move Blocking Strategy for Open Channel Systems

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Dynamic Event-Triggered MPC Enhanced with Time-Varying Move ...

microRNA-210-3p通过调控TET2的表达抑制大鼠炎性疼痛

第43届中国控制会议

作者： chenlong cui Lihui Cen Shaohui Zhang Xiaofang Chen Dewei Li State Key Laboratory of Simulation and Regulation of Water Cycle in River Basin China Institute of Water Resources and Hydropower Research Department of Automation Shanghai Jiao Tong University

This paper explores the Model Predictive Control(MPC) method for open channel systems. By employing spatial discretization, the Saint-Venant equations are transformed into an affine nonlinear model. Building upon this, a dynamic event-triggered MPC algorithm enhanced with time-varying move blocking strategy is proposed. This algorithm integrates the advantages of event-triggered and self-triggered, solving optimization problems only upon event triggers, reducing the solver update frequency. The time-varying move blocking strategy ensures that the obtained control sequence remains constant over a certain time, alleviating computational burdens at each step, and its integration with event-triggered control mitigates the potential impact of frequent gate openings on equipment wear. Recursive feasibility and stability of the Optimization Control Problem(OCP) are ensured. Simulation results validate the effectiveness and efficiency of the proposed algorithm.

关键词： Open Channel Dynamic Event-triggered Model Predictive Control Move blocking

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

山东大学学报（医学版） 2024年第6期62卷 17-29页

作者：卫嘉晟杨保仲魏伟薛亚婷崔臣龙方俊山西医科大学麻醉学院山西太原030001 太原市中心医院麻醉手术科山西太原030006

目的探讨microRNA-210-3p(miR-210-3p)与10-11易位蛋白2(ten-eleven translocation 2,TET2)在完全弗氏佐剂(complete freund's adjuvant,CFA)诱导的大鼠炎性疼痛模型中的作用及其相互调控机制。方法通过生物信息学方法和双荧光素酶实验,分析并确定大鼠miR-210-3p中可以靶向调节的基因。实验中的质粒和miR-210-3p共转染组合分为pmirGLO+mimics NC组、pmirGLO+mimics-miR-210-3p组、TET2-WT-pmirGLO+mimics-NC组、TET2-WT-pmirGLO+mimics-miR-210-3p组、TET2-MT-pmirGLO+mimics-NC组和TET2-MT-pmirGLO+mimics-miR-210-3p组;60只大鼠按随机数字表法分为正常对照(normal control,CON)组(n=20)、CFA组(n=20)、CFA+腺相关病毒载体阴性对照(adeno-associated virus negative control,AAV NC)组(n=10)、CFA+AAV miR-210-3p抑制剂(adeno-associated virus miR-210-3p inhibitor,AAVi)组(n=10)。通过在大鼠左后足底部皮下注入CFA的方式建立大鼠炎性疼痛模型;通过尾静脉注入miR-210-3p inhibitor的AAV建立干预模型;观察并测量大鼠行为学;采用RT-qPCR法检测miR-210-3p的表达量;采用Western blotting法和免疫荧光染色法检测L4~L6腰膨大节段脊髓中TET2蛋白的表达水平及荧光强度的变化;采用免疫荧光染色法观察TET2蛋白在大鼠脊髓中的细胞表达定位。结果生物信息学方法发现,TET2基因3'UTR区域存在与miR-210-3p的结合位点;双荧光素酶报告基因实验证实了miR-210-3p与TET2基因之间存在结合位点,呈负向调控关系;注射CFA显著减小了大鼠的机械缩足反射阈值(paw withdrawal mechanical threshold,PWMT)和热缩足潜伏期(paw thermal withdrawal latency,PTWL)(P<0.05);CFA组大鼠脊髓腰膨大中miR-210-3p的表达水平明显上调,伴随着TET2的表达水平降低(P<0.05);免疫荧光结果显示,TET2蛋白主要和神经元细胞存在共定位:CFA组大鼠脊髓内TET2蛋白表达水平降低(P<0.05);经过AAVi干预后,CFA+AAVi组大鼠在各个时间的PWMT和PTWL较CFA+AAV NC组大鼠升高(P<0.05);CFA+AAVi组大鼠脊髓组织中TET2蛋白表达较CFA+AAV NC组升高(P<0.05)。结论miR-210-3p可以抑制TET2蛋白表达,通过抑制miR-210-3p在炎性疼痛大鼠中的表达可以有效减轻炎性疼痛。

关键词： microRNA-210-3p 10-11易位蛋白2 炎性疼痛完全弗氏佐剂腺相关病毒载体

Biomaterial-assisted photoimmunotherapy for synergistic suppression of cancer progression

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2023年第9期34卷 76-84页

作者： Yanan Li Jing cui chenlong Li Caiting Deng Guowei Deng Hui Zhang Feifei An College of Medical Imaging Shanxi Medical UniversityTaiyuan 030001China College of Public Health Shanxi Medical UniversityTaiyuan 030001China School of Basic Medical Science Health Science CenterXi’an Jiaotong UniversityXi’an 710061China School of Public Health Health Science CenterXi’an Jiaotong UniversityXi’an 710061China College of Chemistry and Life Science Sichuan Provincial Key Laboratory for Structural Optimization and Application of Functional MoleculesChengdu Normal UniversityChengdu 611130China

Photoimmunotherapy is an emerging treatment modality that uses photothermal,photodynamic and photochemical processes to fight against cancer by eliciting a robust host immune ***,various nanoformulations of biomaterials have been rationally designed as highly effective photosensitive agents,immunoadjuvants or carriers to enhance phototherapeutic efficacy,boost immune stimulation,amplify nano-permeability and monitor cancer progression in ***,relying solely on a single-modality therapy may not completely ablate primary tumors,and the metastasis and recurrence of tumors remain a serious *** solve this issue,the strategy of combining photoimmunotherapy with other immunotherapies,such as immune checkpoint blockade,chimeric antigen receptor-T cell or cytokine therapy,can greatly enhance the effectiveness of oncology treatment and reduce the traditional adverse ***,it is very valuable to summarize the research progress in biomaterial-assisted combination photoimmunotherapy for clinical *** this review,the recent advances in constructing multifunctional nano-biomaterials for combinatorial photoimmunotherapy of cancer are ***,the opportunities,challenges,future trends and prospects in this field are also analyzed to pave the way for advancing the next generation of clinical cancer management strategies.

关键词： Biomaterial Phototherapy Immunotherapy Synergistic treatment Cancer management

Development and performance evaluation of the electric-hydraulic concave clearance control system based on maize feed rate mon

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

International Journal of Agricultural and Biological Engineering 2022年第2期15卷 156-164页

作者： chenlong Fan Dongxing Zhang Li Yang Tao cui Xiantao He Huihui Zhao College of Engineering China Agricultural UniversityBeijing 100083China Key Laboratory of Soil-Machine-Plant Systematic Technology Ministry of Agriculture and Rural AffairsBeijing 100083China

Complex field environments,diverse crop conditions,and varying feed rate fluctuations commonly result in a decline in the threshing performance and the clogging of the threshing cylinder for maize *** order to overcome these problems,an electric-hydraulic concave clearance automatic control system for the threshing unit was developed based on the maize feed rate monitoring,which can automatically realize the best match between the concave clearance and diverse feed rates during *** threshing performance of the electric-hydraulic control system was evaluated for varying and uneven maize feed rate fluctuations,such as the feed rate increased(6-8-10 kg/s),the feed rate decreased after an increase(6-10-8 kg/s,8-10-6 kg/s),the feed rate increased after a decrease(8-6-10 kg/s,10-6-8 kg/s),and the feed rate decreased(10-8-6 kg/s).In particular,the threshing rotor shaft peak torque,the range of threshing rotor shaft torque,the rate of broken grains(BGR),and the rate of unthreshed grains(UGR)with and without the electric-hydraulic control system were *** with the electric-hydraulic control system were adjustable concave clearance with the value of 45 mm,50 mm,and 55 *** without the electric-hydraulic control system were constant concave clearance(50 mm).Results demonstrate that the threshing unit with the electric-hydraulic control system outperformed the one without the electric-hydraulic control system,with threshing rotor peak torque,the range of threshing rotor axis torque,the BGR,and the UGR decreasing by 18.38%,38.27%,2.08%,and 0.10%,***,the rate of broken grains was lower than 5.00%,better than the national ***,the feed rate fluctuations and timely adjustment of the concave clearance were able to avoid blocking the rotor and improve the threshing performance compared to the constant concave clearance.

关键词： maize harvester threshing cylinder feed rate concave clearance electro-hydraulic control system

Continuous measurement method and mathematical model for soil compactness

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

International Journal of Agricultural and Biological Engineering 2022年第5期15卷 196-204页

作者： Huihui Zhao Tao cui Li Yang Qingyan Hou Weijun Yan Xiantao He chenlong Fan Jiaqi Dong Dongxing Zhang College of Engineering China Agricultural UniversityBeijing 100083China The Soil-Machine-Plant Key Laboratory of the Ministry of Agriculture of China Beijing 100083China Shandong Guofeng Machinery Co. LtdJining 272000ShandongChina

With the continuous improvement of agricultural mechanization,soil compaction becomes more and more *** soil compaction has been considered as an important negative factor affecting crop growth and *** measurement of soil compactness is a common method to measure the soil compaction *** order to solve the problems of discontinuous sampling,time-consuming and poor real-time soil compactness measurement,a real-time measurement method of soil compactness based on fertilizing shovel was proposed,and the mathematical model between fertilizing shovel arm deformation and soil compactness was *** on the interaction mechanism between fertilizing shovel and soil,through the force analysis of fertilizing shovel,it was found that the deformation of fertilizing shovel arm was positively correlated with the sum of soil compactness(SSC)within the range of tillage *** order to verify the theoretical analysis results and the detection accuracy of strain gauge,the static bench test was carried *** test results showed that the strain gauge signal for measuring the deformation of the fertilizing shovel arm was significantly correlated with the applied *** fitting curve of the linear correlation coefficient was 0.999,the maximum detection error was 0.68 kg,and the detecting accuracy was within the tolerance of 0.57%.Through field orthogonal experiments with four working depths and four compaction levels,a mathematical model of the strain gauge signal and the SSC within the range of tillage depth was *** experiment showed that compared with the other three depths,the linear correlation coefficient at the tillage depth of 5 cm(TD5)was the lowest,and the slope of the fitting curve was obviously different from the other three depths,so the 5 cm data were excluded when *** model between mean signal value and mean SSC within the range of tillage depth was established based on the data of sampling points with tillage dept

关键词： soil compactness measurement fertilizing shovel strain gauge precision agriculture

Continuous measurement method and mathematical model of soil 1 compactness based on the deformation of fertilizing shovel arm

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

International Journal of Agricultural and Biological Engineering 2022年第4期15卷 I0011-I0034页

作者： Huilhui Zhao Dongxing Zhang Li Yang Tao cui Xiantao He chenlong Fan Wentong Liu College of Engineering China Agricultural University17 Qinghua East RoadBeijing 100083China The Soi-Machine Plant key laboratory of the Ministry of Agriculture of China 17 Qinghua East RoadBeijing 100083China

Biographies:Huihui Zhao,PhD candidate,research interest:intelligent control for comn precision planters,Email:zhaohh0220@*** Zhang,PhD,Professor,research interest:full mechanization of cor production,Email:zhangdx@***... 详细信息

关键词： Email deformation soil

RSPSSL:A novel high-fidelity Raman spectral preprocessing scheme to enhance biomedical applications and chemical resolution visualization

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Light(Science & Applications) 2024年第2期13卷 366-386页

作者： Jiaqi Hu Gina Jinna Chen chenlong Xue Pei Liang Yanqun Xiang Chuanlun Zhang Xiaokeng Chi Guoying Liu Yanfang Ye Dongyu cui De Zhang Xiaojun yu Hong Dang Wen Zhang Junfan Chen Quan Tang Penglai Guo Ho-Pui Ho Yuchao Li Longqing Cong Perry Ping Shum State Key Laboratory of Optical Fiber and Cable Manufacture Technology Guangdong Key Laboratory of Integrated Optoelectronics IntellisenseDepartment of EEESouthern University of Science and TechnologyShenzhen 518055China College of Optical and Electronic Technology China Jiliang UniversityHangzhou 310018China Department of Nasopharyngeal Carcinoma Sun Yat-sen University Cancer CenterState Key Laboratory of Oncology in South ChinaCollaborative Innovation Center for Cancer MedicineGuangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and TherapyGuangzhou 510060China Department of Ocean Science and Engineering Southern University of Science and TechnologyShenzhen 518055China Department of Nephrology Chaozhou People’s HospitalChaozhou 521011China Clinical Research Design Division Sun Yatsen Memorial HospitalGuangzhouGuangdong 510120China School of Automation Northwestern Polytechnical UniversityXi’anShaanxi 710072China Department of Biomedical Engineering The Chinese University of Hong KongHong KongChina Guangdong Provincial Key Laboratory of Nanophotonic Manipulation Institute of NanophotonicsJinan UniversityGuangzhou 511443China

Raman spectroscopy has tremendous potential for material analysis with its molecular fingerprinting capability in many branches of science and *** is also an emerging omics technique for metabolic profiling to shape precision ***,precisely attributing vibration peaks coupled with specific environmental,instrumental,and specimen noise is *** Raman spectral preprocessing to remove statistical bias noise and sample-related errors should provide a powerful tool for valuable information ***,we propose a novel Raman spectral preprocessing scheme based on self-supervised learning(RSPSSL)with high capacity and spectral *** can preprocess arbitrary Raman spectra without further training at a speed of~1900 spectra per second without human *** experimental data preprocessing trial demonstrated its excellent capacity and signal fidelity with an 88%reduction in root mean square error and a 60%reduction in infinite norm(L__(∞))compared to established *** this advantage,it remarkably enhanced various biomedical applications with a 400%accuracy elevation(ΔAUC)in cancer diagnosis,an average 38%(few-shot)and 242%accuracy improvement in paraquat concentration prediction,and unsealed the chemical resolution of biomedical hyperspectral images,especially in the spectral fingerprint *** precisely preprocessed various Raman spectra from different spectroscopy devices,laboratories,and diverse *** scheme will enable biomedical mechanism screening with the label-free volumetric molecular imaging tool on organism and disease metabolomics profiling with a scenario of high throughput,cross-device,various analyte complexity,and diverse applications.

关键词： spectral scheme resolution

Verification of DNA Motifs in Arabidopsis using CRISPR/Cas9 Mediated Mutagenesis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Verification of DNA Motifs in Arabidopsis using CRISPR/Cas9 ...