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Peri-centrosomal localization of small interfering RNAs in C. elegans

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Science China Life Sciences 2025年

作者： Qile Jin Xuezhu Feng Minjie Hong Ke Wang Xiangyang Chen Jiewei Cheng Yan Kuang Xiaoyue Si Mingjing Xu Xinya Huang Shouhong Guang chengm.ng zhu Department of Obstetrics and Gynecology The First Affiliated Hospital of USTC The USTC RNA Institute Hefei National Research Center for Physical Sciences at the Microscale School of Life Sciences Division of Life Sciences and Medicine Biomedical Sciences and Health Laboratory of Anhui Province University of Science and Technology of China School of Basic Medicine Anhui Medical University

The centrosome is the microtubule-organizing center and a crucial part of cell division. Centrosomal RNAs(cnRNAs) have been reported to enable precise spatiotemporal control of gene expression during cell division in many species. Whether and how cnRNAs exist in C. elegans are unclear. Here, using the nuclear RNAi Argonaute protein NRDE-3 as a reporter, we observed potential peri-centrosome localized small interfering(si)RNAs in C. elegans. NRDE-3 was previously shown to associate with pre-mRNAs and pre-rRNAs via a process involving the presence of complementary siRNAs. We generated a GFP-NRDE-3 knock-in transgene through CRISPR/Cas9 technology and observed that NRDE-3 formed peri-centrosomal foci neighboring the tubulin protein TBB-2, other centriole proteins and pericentriolar material(PCM)components in C. elegans embryos. The peri-centrosomal accumulation of NRDE-3 depends on RNA-dependent RNA polymerase(RdRP)-synthesized 22G siRNAs and the PAZ domain of NRDE-3, which is essential for siRNA binding. Mutation of eri-1, ergo-1, or drh-3 significantly increased the percentage of pericentrosome-enriched NRDE-3. At the metaphase of the cell cycle, NRDE-3 was enriched in both the peri-centrosomal region and the spindle. Moreover, the integrity of centriole proteins and pericentriolar material(PCM) components is also required for the peri-centrosomal accumulation of NRDE-3. Therefore, we concluded that siRNAs could accumulate in the pericentrosomal region in C. elegans and suggested that the peri-centrosomal region may also be a platform for RNAi-mediated gene regulation.

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Topology modulation of tetraphenylethylene graphdiyne at a liquid-liquid interface

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Nano Research 2024年第6期17卷 4661-4667页

作者： Tingting Xu Pei Wen ng Shaofei Wu Danjun Fu Yunhan Ma Yao Liu Lidao Li Xiaofei Zhang Jun zhu Department of Chemistry National University of SingaporeSingapore 117543Singapore Institute of Materials Research and Engineering(IMRE) Agency for ScienceTechnology and Research(A*STAR)Singapore 138634Singapore

Monomers with low symmetries can form different topological structures in the preparation of organic two-dimensional(2D)***,it remains challenging to modulate the topologies in practical *** theoretical insights into the formation energy of potential structural configurations,we report the topology modulation of a graphdiyne(GDY)derivative constructed from two-fold symmetric tetrakis(4-ethynylphenyl)ethene precursor by changing solvent combinations in a liquid-liquid interfacial *** aqueous-organic(water-dichloromethane)interface afforded GDY with a kagome topology while a rhombic topology was formed at an organic-organic(hexane-acetonitrile)interface.A comprehensive evaluation of their structures and optoelectronic properties was conducted through various characterization techniques and theoretical *** study provided new insights to modulate the topology of not only GDY but also other framework structures and obtain topologically pure materials in situations where different topologies are possible during practical synthesis.

关键词： graphdiyne derivative tetraphenylethylene topology modulation aggregation-induced emission(AIE)two-dimensional(2D)polymers interfacial synthesis

Leader-follower: Human-centered intention-guided controller for novel SuperLimb with application to load-carrying scenarios

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Science China Technological Sciences 2025年第1期 325-338页

作者： Qinghua ZHAng Xianglong LI Hongwei JIng Kerui SUN Yuancheng LI Sikai ZHAO Lele LI Haotian JU Ziqi WAng Rui WU Tianjiao ZHEng Jie ZHAO Yanhe zhu The State Key Laboratory of Robotics and System Harbin Institute of Technology The Learning Algorithms and Systems Laboratory School of Engineeringécole Polytechnique Fédérale de Lausanne

Supernumerary robotic arms(SuperLimb) are a new type of wearable robot that works closely with humans as a third hand to augment human operation capability. Accurate conveyance of wearers' intentions, allocation of roles, and humancentered interaction considerations are the key points in the process of human–SuperLimb collaboration. This paper proposes a human-centered intention-guided leader–follower controller that relies on the dynamic modeling of SuperLimb with application to load-carrying scenarios. The proposed leader–follower controller takes the human as the leader and the SuperLimb as the follower, achieving effective information communication, autonomous coordination, and good force compliance between SuperLimb, humans, and the environment under human safety assurance. First, the human–SuperLimb dynamic system is modeled to achieve force interaction with the environment and wearer. Second, to achieve the precise intention extraction of humans, pose data from five visual odometry sensors are fused to capture the human state, the generalized position, the velocity of hands, and the surface electromyography signals from two myoelectric bracelets sensors are processed to recognize the natural hand gestures during load-carrying scenarios by a designed Swin transformer network. Then, based on the real-time distance detection between human and mechanical limbs, the security assurance and force-compliant interaction of the human–SuperLimb system are realized. Finally, the human hand muscle intention recognition, human–robot safety strategy verification,and comparative load-carrying experiments with and without the proposed method are conducted on the SuperLimb *** showed that the task parameters are well estimated to produce more reasonable planning trajectories, and SuperLimb could well understand the wearer's intentions to switch different SuperLimb actions. The proposed sensor-based human–robot communication framework motivates future studies of o

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智慧医疗情境下四方主体隐私行为的交互机理及演化趋势研究

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现代情报 2025年第1期45卷 135-149页

作者：朱光吴晗逸刘雯南京信息工程大学风险治理与应急决策研究院江苏南京210044 南京信息工程大学管理工程学院江苏南京210044

[目的/意义]本文针对智慧医疗情境下隐私行为研究在博弈主体、模型参数等方面的不足,探寻患者、医疗机构、智慧医疗平台和政府四方主体之间的隐私行为交互机理与演化趋势。[方法/过程]基于演化博弈理论,构建患者、医疗机构、智慧医疗平... 详细信息

[目的/意义]本文针对智慧医疗情境下隐私行为研究在博弈主体、模型参数等方面的不足,探寻患者、医疗机构、智慧医疗平台和政府四方主体之间的隐私行为交互机理与演化趋势。[方法/过程]基于演化博弈理论,构建患者、医疗机构、智慧医疗平台和政府四方博弈模型。根据不同参数调节下各方行为策略的收益、成本、损失等计算收益矩阵,求解主体的演化稳定策略,并考虑不同因素对主体行为策略及系统稳定状态的影响。[结果/结论]患者的隐私披露行为与披露隐私后获得的服务收益密切相关,医疗机构的隐私保护投入成本和隐私泄露概率对其隐私保护行为有显著影响,智慧医疗平台的政府奖惩、罚款收益和投入成本是影响其严格管理行为的关键因素,政府的监管行为与奖惩机制等因素密切相关。通过应用本文模型和方法,可以动态调整隐私保护机制,明确各方职责,推动智慧医疗可持续健康发展。

关键词：智慧医疗演化博弈隐私行为患者医疗机构智慧医疗平台政府

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Contemporary Value and Educational Enlightenment of zhu Xi's Moral Education Thoughts

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学术界 2016年第4期 314-318页

作者： zhu Huiyun Department of Philosophy Baoji University of Arts and Sciences

As a philosopher and educator,zhu Xi is famous for his rich moral education *** formation of these moral education thoughts was not only closely associated with his own moral education activities and academic research,but also indispensible from his own Confucian family background,social context of his time,and his own thoughts and cultural *** Xi’ s moral education thoughts are of great contemporary value,serving as reference for the construction of people’ s values,a harmonious society,civic virtues and civic *** these,his thoughts especially give significant enlightenment to modern education in China.

关键词： zhu Xi moral education thoughts contemporary value

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《民法典》用人者替代责任的司法解释完善

北京理工大学学报(社会科学版) 2025年第1期27卷 44-55页

作者：王竹帅仁策四川大学　法学院四川成都610207

《民法典侵权责任编解释（一）》第15条将“用工单位劳务关系”和“个体工商户用工关系”纳入《民法典》第1191条第1款规定的“用人单位”替代责任的适用范围，但未能明确网络平台用工替代责任的法律适用规则。《民法典》第1191～第119... 详细信息

《民法典侵权责任编解释（一）》第15条将“用工单位劳务关系”和“个体工商户用工关系”纳入《民法典》第1191条第1款规定的“用人单位”替代责任的适用范围，但未能明确网络平台用工替代责任的法律适用规则。《民法典》第1191～第1193条已构建“用人单位劳动关系—个人劳务关系—独立承揽关系”三分法用工关系谱系。应当以第1191条第1款为中心补正用工单位劳务关系替代责任规则的缺失，以第1192条为中心构建个体工商户用工关系替代责任法律适用框架，以第1193条为中心构建网络平台用工替代责任法律适用框架。

关键词：用人者替代责任用人单位用工单位个体工商户网络平台用工

Bifunctional bridging capping layer enables 24.5% efficiency of perovskite solar cells with polymer-based hole transport materials

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Science China Chemistry 2025年第1期 350-359页

作者： Can zhu Yiyang Wang Lei Meng Beibei Qiu Jing Li Shucheng Qin Ke Hu Xin Jiang Wenbin Lai Minchao Liu Zhe Liu Chenxing Lu Jinyuan Zhang Yongfang Li Beijing National Laboratory for Molecular Sciences CAS Key Laboratory of Organic Solids Institute of Chemistry Chinese Academy of Sciences School of Chemical Science University of Chinese Academy of Sciences Key Laboratory of Solid State Optoelectronic Devices of Zhejiang Province College of Physics and Electronic Information Engineering Zhejiang Normal University Key Laboratory of Photochemical Conversion and Optoelectronic Materials Technical Institute of Physics and ChemistryChinese Academy of Sciences Laboratory of Advanced Optoelectronic Materials College of Chemistry Chemical Engineering and Materials ScienceSoochow University

Developing a bridge capping layer between perovskite and hole transport layer materials（HTMs） in the n-i-p perovskite solar cells（pero-SCs） is an effective approach to modify the morphology of HTMs and passivate the perovskite ***, we select the quinoxaline-based bifunctional passivation agent, quinoxalin-6-yl-methylamine hydrochloride（Qx MACl）,as the bridging layer, and a D-A copolymer PBQ12 containing the same quinoxaline unit as an HTM for the n-i-p pero-SCs. Due to the π-π stacking among the common quinoxaline units in the bridge layer and HTM, Qx MACl induces the π-π stacking of the PBQ12 film and improves the film morphology of HTMs with better conductivity. Additionally, Qx MACl can effectively passivate the perovskite surface, and PBQ12 possesses appropriate energy levels and high hole mobility. The pero-SCs based on FAPbI3with PBQ12/Qx MACl treatment showed a higher power conversion efficiency（PCE） of 24.05% and outstanding stability, maintaining 95.4% and 92.1% of its initial PCE after 750 h of storage and after over 800 h of thermal annealing at 85 °C, respectively. To further enhance the PCE of the PBQ12/Qx MACl-based devices, we developed a non-metal ion dopant for the PBQ12 HTM. Through trace doping of PBQ12 HTM by the non-metal ion dopant, the PCE of the PBQ12/Qx MACl-based devices reached 25.24%（the calibrated PCE of 24.55% by the National Institute of Metrology, China）.

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Tuning the porosity of {Co12IICo8III（μ3-OH）24（X）}11+ supramolecular frameworks by reaction time for D2/H2 separation

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Science China Chemistry 2025年

作者： zhuozhou Xie zhu zhuo Zi-Ang Nan Qing Li Xiufang Li Zi-Xiu Lu Linzhe Lü Jin Liu Luyao Liu Wenjing Wang Wei Wang Daqiang Yuan You-Gui Huang State Key Laboratory of Structure Chemistry Fujian Institute of Research on the Structure of Matter Chinese Academy of Sciences University of Chinese Academy of Sciences Xiamen Key Laboratory of Rare Earth Photoelectric Functional Materials Xiamen Institute of Rare Earth Materials Haixi InstitutesChinese Academy of Sciences State Key Laboratory of Physical Chemistry of Solid Surfaces Department of Chemistry College of Chemistry and Chemical EngineeringXiamen University

Systematicly tuning the porosities of porous materials is crucial for targeted gas mixture separation,yet it remains a longstanding *** a common strategy,altering the phenylene ring count in the organic building units has been exploited to adjust pore apertures in metal organic frameworks （MOFs） and covalent organic frameworks （COFs）,resulting in only a stepwise pore size variation of approximately 2.8?.Unlike MOFs and COFs,the porosities for supramolecular assembly frameworks are highly dependent on the packings of the molecular building units in the *** above strategy cannot be applied to supramolecular assembly frameworks and other strategies are required to adjust pore apertures in supramolecular frameworks,especially to achieve porous materials with the optimal pore size for D2/H2separation （～3?）.Here,we successfully modulate the porosity of supramolecular frameworks of[CoII1+12CoIII8L12（μ3-OH）24（X）]1clusters （L=mono-deprotonated 1-iminoisoindole-3-amine,X=ClO?4or Cl?） by controlling the duration of self-assembly *** simply adjusting the reaction time,we obtain three[CoII12CoIII8L12（μ3-OH）24（X）]11+supramolecular frameworks:[CoII12CoIII8（μ3-OH）24（ClO4）L12)]·（Cl）4·（HCO2）7（1）,[CoII12CoIII8L12（μ3-OH）24（Cl）]·（ClOI4）4·（Cl）6·（HCO2）·（H2O）10（2）,and[CoI12CoIII8L12（μ3-OH）24（Cl）]·（Cl）11·（H2O）12（3）.The different stacking patterns of the[CoII12CoIII8L12（μ3-OH）24（X）]11+clusters in these frameworks lead to significantly varied stabilities and *** 1 exhibits permanent porosity with interconnected channels and maintains stability under harsh *** contrast,compound 2 is nearly nonporous,and compound 3 becomes unstable upon *** pores of compound 1 show higher affinity to D2than H2,and the pore aperture diameter of compound 1 （～3.06?） meets the optimal porosity for D2/***,compound 1 demonstrates moderate D2/H2separation at 77 K (retention time:～7 min/g for D2/H2/Ne （10/10/80 vol%） in a flow of 5 mL

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[Determination of 12 halogenated organic pollutants in edible fish by ultra performance liquid chromatography-high resolution mass spectrometry combined with ultrasound-assisted extraction and gel permeation chromatography purification]

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Se pu = Chinese journal of chromatography 2025年第1期43卷 68-77页

作者： Yi-Zhe zhu Rui-Fen Zheng Zi-Hao Fan Ling Liu Jing-Yao Ye Kai Wang Cai-Ming Tang Research Center for Eco-Environmental Engineering Dongguan University of Technology Dongguan 523808 China. Guangdong Key Laboratory of Environmental Resources Utilization and Protection Guangzhou Institute of Geochemistry Chinese Academy of Sciences Guangzhou 510640 China.

Halogenated organic pollutants (HOPs) have attracted considerable attention owing to their persistence, bioaccumulation, and toxicity. The development of methods to detect HOPs in fish is challenging owing to the compositional complexity of fish matrices, which contain high levels of lipids and relatively low concentrations of HOPs. In addition, the lipophilicity of most HOPs renders their extraction difficult. Moreover, the simultaneous determination of multiple HOPs to achieve the high-throughput screening of these analytes is complex. In this study, a reliable and efficient pretreatment method based on ultrasound-assisted extraction, gel permeation chromatography purification, and ultra performance liquid chromatography-high resolution mass spectrometry (UPLC-HRMS) was developed for the determination of 12 HOPs in edible fish. The procedures of sample extraction and purification and LC-HRMS detection parameters were optimized to improve the performance of the method. Fresh fish samples were thoroughly rinsed with water, and non-edible parts, including the skin, bones, and phosphorus, were removed. The fish were weighed, cut into small pieces, and vacuum freeze-dried for 48 h. Subsequently, a freeze grinder was used to grind the dried fish into a fine powder. Exactly 2 g of the fish powder was weighed, fortified with isotope-labeled internal standards of the HOPs, and allowed to stand for 5 min. Methanol-acetonitrile (1∶1, v/v) was then added, followed by vortex mixing and ultrasonication. After centrifugation, the supernatant was transferred to a fresh tube. The extraction process was repeated twice and all extracts were combined. The extract was evaporated under a gentle nitrogen flow and redissolved in a mixture of ethyl acetate-cyclohexane (1∶1, v/v). The sample mixture was cleaned using gel permeation chromatography, and the eluate was collected and concentrated under a nitrogen flow. Sample residuals were reconstituted with water-methanol (1∶1, v/v) prior to

关键词： edible fish gel permeation chromatography (GPC) halogenated organic pollutants (HOPs) ultra performance liquid chromatography-high resolution mass spectrometry (UPLC-HRMS)