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Scanning electron microscopy coupled with energydispersive X-ray spectrometry for quick detection of sulfuroxidizing bacteria in environmental water samples

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Chinese Journal of Oceanology and Limnology 2017年第1期35卷 185-191页

作者： sun chengjun JIANG Fenghua GAO Wei LI Xiaoyun YU Yanzhen YIN Xiaofei WANG Yong DING Haibing Marine Ecology Center First Institute of Oceanography State Oceanic Administration Qingdao 266061 China College of Chemistry and Chemical Engineering Ocean University of China Qingdao 266100 China Qingdao Hao Ao Environmental Engineering Ltd. Qingdao 266100 China Key Laboratory of Marine Chemistry Theory and Technology Ministry of Education Ocean University of China Qingdao 266100 China Qingdao Collaborative Innovation Center of Marine Science and Technology Ocean University of Chino Qingdao 266100 China

Detection of sulfur-oxidizing bacteria has largely been dependent on targeted gene sequencing technology or traditional cell cultivation, which usually takes from days to months to carry out. This clearly does not meet the requirements of analysis for time-sensitive samples and/or complicated environmental samples. Since energy-dispersive X-ray spectrometry(EDS) can be used to simultaneously detect multiple elements in a sample, including sulfur, with minimal sample treatment, this technology was applied to detect sulfur-oxidizing bacteria using their high sulfur content within the cell. This article describes the application of scanning electron microscopy imaging coupled with EDS mapping for quick detection of sulfur oxidizers in contaminated environmental water samples, with minimal sample handling. Scanning electron microscopy imaging revealed the existence of dense granules within the bacterial cells, while EDS identified large amounts of sulfur within them. EDS mapping localized the sulfur to these granules. Subsequent 16S rRNA gene sequencing showed that the bacteria detected in our samples belonged to the genus Chromatium, which are sulfur oxidizers. Thus, EDS mapping made it possible to identify sulfur oxidizers in environmental samples based on localized sulfur within their cells, within a short time(within 24 h of sampling). This technique has wide ranging applications for detection of sulfur bacteria in environmental water samples.

关键词： sulfur bacteria energy-dispersive X-ray spectrometry scanning electron microscopy bacteria detection environmental water samples 16S rRNA sequencing

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Degradation potential and diversity of oil-degrading bacteria isolated from the sediments of the Jiaozhou Bay, China

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Acta Oceanologica Sinica 2019年第6期38卷 54-64页

作者： Wei Gao Xiangxing Gao Tiezhu Mi Bin Han Yiran Zhang Xinzi Li Xiaofei Yin chengjun sun Qian Li Zhisong Cui Xiao Luan Zhigang Yu Li Zheng College of Marine Life Sciences Ocean University of ChinaQingdao 266003China Marine Ecology Research Center First Institute of OceanographyMinistry of Natural ResourcesQingdao 266061China National Deep Sea Center Qingdao 266237China College of Environmental Science and Engineering Ocean University of ChinaQingdao 266100China Key Laboratory of Marine Chemical Theory and Technology Ministry of EducationQingdao 266100China Laboratory for Marine Ecology and Environmental Science Pilot National Laboratory for Marine Science and Technology (Qingdao)Qingdao 266237China

A great deal of oil contaminated the shoreline by the Qingdao oil pipeline explosion in 2013. The four oildegrading consortia were enriched from sediment samples with crude oil as sole carbon and energy sources. The biodiversity and community analysis showed that the Luteibacter, Parvibaculum and a genus belonging to Alcanivoracaceae were found predominant bacteria in the four consortia, which belonged to Proteobacteria. Nine strains exhibiting distinct 16S rRNA gene sequences were isolated from the consortia. These strains were identified to eight genera based on 16S rRNA gene sequences. Five of the nine strains degraded more than 30% of the crude oil in two weeks by gravimetric method. From the analysis of GC-MS, most of the isolated strains tended to degrade n-alkanes rather than PAHs. Five strains showed high degrading ability of the total n-alkanes. Only Strain D2 showed great PAHs degrading ability and the degrading rates ranged from 34.9% to 77.5%. The sequencing analysis of the oil-degrading consortia confirmed that the genus of Alcanivorax was one of the dominant bacteria in Consortia A and E and Strain E4 might be one of the dominant bacteria. The strains obtained in this study demonstrated the potential for oil bioremediation in oil-contaminated beach ecosystems.

关键词： oil spill biodegradation microbial consortium dominant bacteria

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New yellow Ba_(0.93)Eu_(0.07)Al_(2)O_(4) phosphor for warm-white light-emitting diodes through single-emitting-center conversion

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Light(Science & Applications) 2013年第1期2卷 226-233页

作者： Xufan Li John D Budai Feng Liu Jane Y Howe Jiahua Zhang Xiao-Jun Wang Zhanjun Gu chengjun sun Richard S Meltzer Zhengwei Pan College of Engineering University of GeorgiaAthensGA 30602USA Department of Physics and Astronomy University of GeorgiaAthensGA 30602USA Materials Science and Technology Division Oak Ridge National LaboratoryOak RidgeTN 37831USA State Key Laboratory of Luminescence and Applications Changchun Institute of OpticsFine Mechanics and PhysicsChinese Academy of SciencesChangchun 130033China Department of Physics Georgia Southern UniversityStatesboroGA 30460USA Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety Institute of High Energy PhysicsChinese Academy of SciencesBeijing 100049China X-ray Science Division Argonne National LaboratoryArgonneIL 60439USA

Phosphor-converted white light-emitting diodes for indoor illumination need to be warm-white(i.e.,correlated color temperature,4000 K)with good color rendition(i.e.,color rendering index.80).However,no single-phosphor,single-emitting-center-converted white light-emitting diodes can simultaneously satisfy the color temperature and rendition requirements due to the lack of sufficient red spectral component in the phosphors’emission ***,we report a new yellow Ba_(0.93)Eu_(0.07)Al_(2)O_(4) phosphor that has a new orthorhombic lattice structure and exhibits a broad yellow photoluminescence band with sufficient red spectral ***-white emissions with correlated color temperature,4000 K and color rendering index.80 were readily achieved when combining the Ba_(0.93)Eu_(0.07)Al_(2)O_(4) phosphor with a blue light-emitting diode(440–470 nm).This study demonstrates that warm-white light-emitting diodes with high color rendition(i.e.,color rendering index.80)can be achieved based on single-phosphor,single-emitting-center conversion.

关键词： luminescence single-phosphor-conversion warm-white light-emitting diode yellow phosphor

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Acoustofluidics-enhanced biosensing with simultaneously high sensitivity and speed

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Microsystems & Nanoengineering 2024年第3期10卷 335-346页

作者： Yuang Li Yang Zhao Yang Yang Wenchang Zhang Yun Zhang Sheng sun Lingqian Zhang Mingxiao Li Hang Gao chengjun Huang Institute of Microelectronics of the Chinese Academy of Sciences Beijing 100029P.R.China University of Chinese Academy of Sciences Beijing 101408P.R.China Department of Medicine Brigham and Women’s HospitalHarvard Medical SchoolBostonMA 02115USA

Simultaneously achieving high sensitivity and detection speed with traditional solid-state biosensors is usually limited since the target molecules must passively diffuse to the sensor surface before they can be *** techniques have been applied to shorten the diffusion time by continuously moving molecules through the biosensing ***,the binding efficiencies of the biomolecules are still limited by the inherent laminar flow inside microscale *** this study,focused traveling surface acoustic waves were directed into an acoustic microfluidic chip,which could continuously enrich the target molecules into a constriction zone for immediate detection of the immune reactions,thus significantly improving the detection sensitivity and *** demonstrate the enhancement of biosensing,we first developed an acoustic microfluidic chip integrated with a focused interdigital transducer;this transducer had the ability to capture more than 91%of passed ***,polystyrene microbeads were pre-captured with human IgG molecules at different concentrations and loaded for detection on the *** representative results,~0.63,2.62,11.78,and 19.75 seconds were needed to accumulate significant numbers of microbeads pre-captured with human IgG molecules at concentrations of 100,10,1,and 0.1 ng/mL(~0.7 pM),respectively;this process was faster than the other methods at the hour level and more sensitive than the other methods at the nanomolar *** results indicated that the proposed method could significantly improve both the sensitivity and speed,revealing the importance of selective enrichment strategies for rapid biosensing of rare molecules.

关键词： fluid continuously simultaneous

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The on-chip thermoelectric cooler:advances,applications and challenges

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Chip 2024年第2期3卷 96-108页

作者： chengjun Li Yubo Luo Wang Li Boyu Yang Chengwei sun Wenyuan Ma Zheng Ma Yingchao Wei Xin Li Junyou Yang State Key Laboratory of Materials Processing and Die&Mould Technology School of Materials Science and EngineeringHuazhong University of Science and TechnologyWuhan 430074China

With the development of 5G technology and increasing chip integration,traditional active cooling methods struggle to meet the growing thermal demands of *** coolers(TECs)have garnered great attention due to their rapid response,significant cooling differentials,strong compatibility,high stability and controllable device *** this review,starting from the fundamental principles of thermoelectric cooling and device design,high-performance thermoelectric cooling materials are summarized,and the progress of advanced on-chip TECs is comprehensively ***,the paper outlines the challenges and opportunities in TEC design,performance and applications,laying great emphasis on the critical role of thermoelectric cooling in addressing the evolving thermal management requirements in the era of emerging chip technologies.

关键词： Chip Thermal management Thermoelectric materials Thermoelectric cooler Cooling performance

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The second-generation sequencing for analyzing mussel adhesive protein transcriptome

The second-generation sequencing for analyzing mussel adhesi...

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“全球变化下的海洋与湖沼生态安全”学术交流会

作者： YU Yanzhen ZHU Yaoyao JIANG Fenghua sun chengjun Marine Ecology Center The First Institute Of Oceanography. SOA Marine Chemistry Ocean University of China

Mussels can tightly attach to any substrate and in this process a number of adhesive proteins are expressed. These proteins play important roles in byssus formation and mussel adhesion. By using the second generation sequencing technology of Illumina Solexa, mussel foot transcriptome was measured and analyzed in order to identify adhesive protein gene. We analyzed the expression of mussel adhesive proteins through transcription of de novo. A number of adhesive proteins were identified. The importance of applying transcriptome to quickly screen and identify functional proteins is discussed.

关键词： Mussel adhesive protein transcriptome analysis

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用氧瓶燃烧-电位滴定法测定煤中有机硫和橡胶总硫

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分析测试学报 1999年第3期18卷 39-42页

作者：孙贤祥孙成俊江苏石油化工学院应用化学系中国人民解放军防化指挥工程学院

研究了煤中有机硫和橡胶中总硫的微量测定方法，样品经氧瓶分解后所转变的ＳＯ４２－在Ｆｅ（ＣＮ）６３－／Ｆｅ（ＣＮ）６４－电对存在下用Ｐｂ２＋滴定。提出了采用Ｚｎ３［Ｆｅ（ＣＮ）６］２、微酸性溶液和低浓度Ｆｅ（ＣＮ）６４... 详细信息

研究了煤中有机硫和橡胶中总硫的微量测定方法，样品经氧瓶分解后所转变的ＳＯ４２－在Ｆｅ（ＣＮ）６３－／Ｆｅ（ＣＮ）６４－电对存在下用Ｐｂ２＋滴定。提出了采用Ｚｎ３［Ｆｅ（ＣＮ）６］２、微酸性溶液和低浓度Ｆｅ（ＣＮ）６４－存在下，消除磷干扰的新方法，应用于合成样品和实际样品的分析，结果满意。其回收率＞９８％，相对标准偏差＜２．３％，绝对误差＜０．１５％。

关键词：有机硫电位滴定煤橡胶硫测定

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Analysis and Simulation for Capacity of Time Division-Synchronous Code Division Multiple Access System

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Journal of Systems Engineering and Electronics 2002年第4期13卷 91-97页

作者： sun chengjun, Zhang Zhongzhao, Ba Yong2 & Cao Pengzhi (Communication Research ("enter, Harbin Institute of Technology, Harbin 150001, P. R. China Department of Electronic Engineering, Tsinghua University, Beijing 100084, P. R. China Research and Development Division, Shanghai Bell Company Limited, Shanghai 200092, P. R. China) Communication Research Center Harbin Institute of Technology Harbin 150001 P. R. China Department ot Electronic Engineering Isinghua University Beijing 100084 P. R. China Research and Development Division Shanghai Bell Company Lirmted Shanghai 200092 P. R. China

Capcity both in uplink and downlink of TD-SCDMA (time division-synchronous code division multiple access) system is studied in a multi-cell environment. The theoretical expressions of the mean of intercell interference in uplink and the mean of sum of power allocation in downlink are given, by which uplink and downlink capacity is analyzed. Furthermore, we give the simulation models for both uplink and downlink capacity. The results from theoretical analysis and simulation fit very well. In the end, the maximum number of users that TD-SCDMA system can serve for 12.2 k speech service is given.

关键词： Code division multiple access Probability Resource allocation Simulation Time division multiple access

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Detection of Promoter Methylation Status of Suppressor of Cytokine Signaling 3(SOCS3) in Tissue and Plasma from Chinese Patients with Different Hepatic Diseases

Detection of Promoter Methylation Status of Suppressor of Cy...

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中国生物化学与分子生物学会第十二届全国会员代表大会暨2018年全国学术会议

作者： Ling Wei Yuan Huang Jing zhang Qiuying Liu Xueqin Ding chengjun sun Jingyang He Xiaoqin Yu Zhongjian Liu Aimin sun Yang Qin Department of Biochemistry and Molecular Biology West China School of Basic Medical Sciences & Forensic MedicineSichuan University West China College of Public Health Sichuan University Analytical & Testing Center Sichuan University

SOCS3 as an important negative regulator of IL6/JAK/STAT3 signaling pathway may be early critical determinants of *** study aimed to explore the aberrant promoter methylation of SOCS3 gene in circulating DNA as a noninvasive biomarker for screening hepatocellular carcinoma(HCC) high risk individuals and for prognosis of HCC patients after partial *** detected its methylation status in 116 liver tissues and 326 plasma specimens of different hepatic diseases and healthy subjects,and its mRNA and protein expression in *** methylation rate was remarkably detected in HCC(47.92%),compared with corresponding nontumor(25.0%),liver cirrhosis(LC)(10.0%),benign liver diseases(0%) and normalliver tissues(0%)(all P<0.05).SOCS3 mRNA level was significantly lower in methylated HCC tissues(P < 0.05).The expressions of SOCS3 and pSTAT3 were affected by methylation *** and consistency of SOCS3 methylation were found between cancer tissue and corresponding plasma(P < 0.001,κ=0.747).The detection rate of plasma for HCC reached 73.91%,SOCS3 methylation status both in tissue and plasma was significantly associated with AFP400,tumor size,tumor differentiation,metastasis and recurrence(all P<0.05).Patients with SOCS3 methylation were followed up a markedly poorer prognosis than those unmethylated for disease-free survival(P<0.05).These data indicate that methylation status of SOCS3 in plasma cell-free DNA can correctly reflect that in tissue DNA and be used as a noninvasive potential biomarker for poor prognosis of HCC.

关键词： hepatocellular carcinoma SOCS3 methylation plasma prognosis

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受海洋贝类生物启发的界面设计

受海洋贝类生物启发的界面设计

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2001北京国际粘接技术研讨会

作者： chengjun sun J.Herbert Waite MCDB Department Unviersity of CaliforniaSanta Barbara Santa BarbaraCA 93105

对贝类足丝机械性能的研究和关于贝类足丝形成的蛋白质的功能性研究将有助于加深对大分子结构和机械性能间关系的认识,为防污提供信息并有助于设计特殊功能的新型材料。

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