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Towards the understanding from sea surface to hadal zone—A multidisciplinary study of the Yap Trench

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Journal of Oceanology and Limnology 2020年第3期38卷 591-592页

作者： DING Haibing sun chengjun Key Laboratory of Marine Chemistry Theory and Technology Ocean University of ChinaQingdao 266100China Qingdao National Laboratory for Marine Science and Technology Qingdao 266237China Qingdao Collaborative Innovation Center of Marine Science and Technology Qingdao 266100China Key Laboratory of Marine Eco-environmental Science and Technology Marine Bioresource and Environment Research CenterFirst Institute of OceanographyMinistry of Natural ResourcesQingdao 266061China

The water column in the open ocean can be divided into epipelagic(photic),mesopelagic,bathyal,abyssal and hadal zones by *** recent years,more and more research has been focusing on the marine environment below photic zone,especially the deep *** trenches are considered as the deepest part of the ocean,providing ideal places for studying the physical,chemical,biological,and geological aspects of the marine environment from sea surface to the deep sea fl ***,limited by the high diffi culty to access and high cost in exploration,our understanding about oceanic trenches is still ***,with the development of series of manned and unmanned submersibles,we now have more opportunities to study the trench area.

关键词： ocean oceanic trench

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常见塑料薄膜在大气环境下的老化特征研究

海洋环境科学 2024年第2期43卷 273-281,291页

作者：蒋凤华刘心好王嘉蔚曹为孙承君自然资源部第一海洋研究所海洋生物资源与环境研究中心山东青岛266061 崂山实验室海洋药物与生物制品功能实验室山东青岛266071 香港理工大学应用生物及化学科技学系中国香港999007

塑料垃圾的老化降解是环境微塑料的重要来源之一。本文选取生活中常用的快递包装袋、外卖包装袋、垃圾包装袋等聚乙烯塑料袋、生物基环保塑料袋和全生物降解塑料袋在室外开展为期16周的暴露实验,通过力学性能测试,结合红外光谱和扫描电... 详细信息

塑料垃圾的老化降解是环境微塑料的重要来源之一。本文选取生活中常用的快递包装袋、外卖包装袋、垃圾包装袋等聚乙烯塑料袋、生物基环保塑料袋和全生物降解塑料袋在室外开展为期16周的暴露实验,通过力学性能测试,结合红外光谱和扫描电镜分析,探究它们在老化降解过程中的特征变化。结果显示,快递包装袋薄膜和外卖包装袋薄膜的断裂伸长率在第5周时迅速降低,垃圾包装袋薄膜和全生物降解塑料袋薄膜的断裂伸长率在第2周迅速降低,垃圾包装袋薄膜第7周之后无法进行力学性能测试,生物基环保塑料袋薄膜的断裂伸长率在实验期间变化不大;5种塑料薄膜的拉伸强度均随老化时间增加而逐渐降低。在老化过程中聚合物与氧气和水发生氧化反应产生羰基、乙烯基和羟基等降解产物,各塑料薄膜的羰基指数、乙烯基指数和羟基指数的变化基本相同,随老化时间增加均呈增加或先增加后降低的变化趋势,而结晶指数变化趋势不明显。经过大气老化后,快递包装袋薄膜和外卖包装袋薄膜表面变得粗糙,出现孔洞和裂纹;垃圾包装袋薄膜表面划痕增加,出现细小白色颗粒;生物基环保塑料袋薄膜表面的淀粉微球剥落并出现分层;全生物降解塑料袋薄膜表面裂纹坑洞变多,颗粒剥落。该结果可为研究包装用塑料袋薄膜的环境行为过程、微塑料来源及其环境风险评估等提供基础数据。

关键词：塑料大气老化拉伸性能红外光谱扫描电镜

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骨肉瘤多组学研究进展

肿瘤学杂志 2024年第5期30卷 419-425页

作者：王磊鑫孙承军武汉科技大学附属华润武钢总医院湖北武汉430080

骨肉瘤(osteosarcoma,OS)是最常见的原发性恶性骨肿瘤,具有高侵袭性和转移性。随着高通量测序技术的发展,组学综合分析在临床应用上变得更为便捷,为多种肿瘤治疗提供了新途径。在OS中,丰富的转录组学、基因组学、表观遗传学、蛋白质组... 详细信息

骨肉瘤(osteosarcoma,OS)是最常见的原发性恶性骨肿瘤,具有高侵袭性和转移性。随着高通量测序技术的发展,组学综合分析在临床应用上变得更为便捷,为多种肿瘤治疗提供了新途径。在OS中,丰富的转录组学、基因组学、表观遗传学、蛋白质组学数据提供了基因表达、基因突变、化学修饰等宝贵信息,有助于更深入地了解OS的分子机制和生物学特征。此外,新兴的单细胞测序能在单细胞水平上定量分析基因表达,揭示不同细胞类型的分子特征,比较OS细胞差异,甚至探究OS的起源等。回顾目前基因组学、转录组学、表观遗传学、蛋白质组学以及新兴的单细胞组学在OS上的研究进展,有助于更全面了解OS发生、进展机制,同时有望发现潜在的治疗靶点,为OS的临床治疗提供重要指导和启示。

关键词：骨肉瘤基因组学转录组学表观遗传学单细胞测序治疗靶点

Water characteristics of abyssal and hadal zones in the southern Yap Trench observed with the submersible Jiaolong

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Journal of Oceanology and Limnology 2020年第3期38卷 593-605页

作者： LIU Xuehai LIU Yongzhi CAO Wei sun chengjun First Institute of Oceanography Ministry of Natural ResourcesQingdao 266061China Laboratory for Regional Oceanography and Numerical Modeling Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China Key Laboratory of Marine Science and Numerical Modeling Ministry of Natural ResourcesQingdao 266061China

Using observations in an applied cruise of the submersible Jiaolong,water characteristics,geostrophic transport,and turbulent mixing in abyssal and hadal zones of the southern Yap Trench were *** spatial structures of deep water show that the abyssal water is cold,saline,and oxygen *** hadal water has very small changes in potential temperature and potential density,and a little decrease in salinity and obvious decrease in *** isotherm,isopycnal,and isohaline are depressed in abyss over the central *** turbulent mixing is enhanced in the near-bottom zone and the hadal water on the trench slope,especially at the steep slope,the dissipation rate and diffusivity is strong,which weakens the *** geostrophic flows move southward in the western region of the trench and northward in the eastern region,indicating cyclonic *** the central region of the trench,the water transport is^1.74 Sv *** the hadal zone,the northward and southward transports are *** analysis suggests that the abyssal water in the southern Yap Trench is from Lower Circumpolar Water(LCPW)and the hadal water seems to be of the isolated local water rather than LCPW.

关键词： Yap Trench abyssal and hadal the submersible Jiaolong diapycnal mixing geostrophic flows

Intermediate filament dynamics： Disassembly regulation

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International Journal of Biomathematics 2017年第1期10卷 273-294页

作者： chengjun sun Julien Arino Stephanie Portet School of Management and EconomicsKunming University of Science and Technology Kunming P. R. China Department of Mechanical Engineering University of Hong Kong Pokfulam Road Hong Kong Department of Mathematics University of Manitoba Winnipeg Manitoba R3T 2N2 Canada

A mechanism of intermediate filament disassembly regulation is proposed in which disas- sembly is regulated by the amount of proteins assembled in networks. It is Mso hypothe- sized that a delay might exist between regulation and actuM disassembly. Under realistic biological conditions of assembly and disassembly, it is shown that such a delay is harm- less and does not destabilize the organization of intermediate filaments in networks. However, for high rates of disassembly, the model predicts that delay can destabilize the organization, with the intermediate filament material oscillating between organizations mainly in networks and in nonfilamentous particles.

关键词： Cytoskeleton organization intermediate filaments time delay global asymp-totic stability bifurcation periodic solution.

AB-8大孔树脂-C18柱联用分离干腌牛肉抗氧化肽的研究

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食品与发酵工业 2024年第5期50卷 171-179页

作者：张迎阳陈文涛徐莹孙承骏邹林玲邹平满在伟常州大学药学院/生物与食品工程学院江苏常州213164

该文利用盐酸提取法提取了干腌牛肉多肽,借助AB-8型大孔树脂及C18球形硅胶柱的分离纯化出4种抗氧化肽,利用分子对接模拟了4种抗氧化肽与超氧化物歧化酶(superoxide dismutase,SOD)的结合位点。利用盐酸提取法提取了抗氧化肽并测试其在1... 详细信息

该文利用盐酸提取法提取了干腌牛肉多肽,借助AB-8型大孔树脂及C18球形硅胶柱的分离纯化出4种抗氧化肽,利用分子对接模拟了4种抗氧化肽与超氧化物歧化酶(superoxide dismutase,SOD)的结合位点。利用盐酸提取法提取了抗氧化肽并测试其在1、2、3、4、5 mg/mL的抗氧化能力,发现在5 mg/mL时,DPPH自由基、ABTS阳离子自由基和超氧阴离子自由基(·O_(2)^(-))清除率分别为86.24%、35.62%和54.34%。对比了AB-8、D101、S-8、X-S、HPD-500、NKA-9的吸附率与解吸率,AB-8的吸附率与解吸率最好,分别为92.55%和82.88%。研究优化了AB-8的上样流速、上样量、乙醇洗脱浓度和洗脱流速,在4 BV/h上样流速、39 mL上样量、75%(体积分数)乙醇溶液洗脱剂和1 mL/min洗脱流速时,可以分离出3个组分(A、B和C),测试3个组分的抗氧化能力,B组分的DPPH自由基、ABTS阳离子自由基和·O_(2)^(-)清除能力最高,分别为73.33%、21.01%和63.33%。对B组分进行C18柱分离,分离出4个抗氧化肽:FDGDF、TGPGPW、FLSDH和KPFDAK。与SOD(PDB ID;1E9O)进行分子对接,发现FDGDF、TGPGPW、FLSDH和KPFDAK均可以与SOD形成氢键,结合能分别为-6.7、-6.55、-6.6、-7.35 kcal/mol,1 mg/mL的FDGDF可以有效增加SOD活力11.64%,酶活力单位增加697 U/mL,研究结果为干腌牛肉制品的开发提供理论基础。

关键词：干腌牛肉抗氧化肽大孔树脂分离纯化分子对接

S-8大孔树脂-C18柱联用分离牡丹籽壳中短叶松素及抗氧化研究

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食品与发酵工业 2024年第6期50卷 152-158页

作者：张迎阳邹林玲陈文涛孙承骏邹平徐莹满在伟常州大学药学院/生物与食品工程学院江苏常州213164

以牡丹籽壳为原料,利用大孔树脂和C18反相键合硅胶柱(C18柱)联合分离短叶松素,回收率为(98.33±0.64)%。用70%(体积分数)乙醇提取牡丹籽壳粗黄酮(Mu Dan Ke Flavonoids,MDKF),得率最高为(10.54±0.13)%;比较了6种大孔树脂(AB-8、S-8、DM... 详细信息

以牡丹籽壳为原料,利用大孔树脂和C18反相键合硅胶柱(C18柱)联合分离短叶松素,回收率为(98.33±0.64)%。用70%(体积分数)乙醇提取牡丹籽壳粗黄酮(Mu Dan Ke Flavonoids,MDKF),得率最高为(10.54±0.13)%;比较了6种大孔树脂(AB-8、S-8、DM301、HPD600、HPD100和D101)的吸附率和解析率,发现S-8大孔树脂的吸附率和解析率最佳,分别为83.47%和84.46%;优化S-8大孔树脂分离MDKF的最佳条件为:上样液质量浓度1.6 mg/mL,上样液流速2.0 mL/min,洗脱剂乙醇体积分数60%,洗脱液流速1.5 mL/min,洗脱液体积100 mL;对经过C18柱分离后的组分进行LC-MS分析得到短叶松素,回收率为(98.33±0.64)%;分离前后抗氧化能力比较:C18纯化物>S-8大孔树脂纯化物>MDKF粗提物;分子对接实验表明,短叶松素与超氧化物歧化酶、过氧化氢酶、过氧化物酶、谷胱甘肽过氧化物酶均有结合能力,过氧化氢酶的结合能力最强为-9.1 kcal/mol。实验表明,短叶松素具有较好的抗氧化能力,可作为食品、药品或化妆品等抗氧化剂的添加,应用前景非常广泛,对牡丹籽壳的进一步开发利用提供理论支持。

关键词：牡丹籽壳短叶松素大孔树脂 C18 分子对接

A Review of Microplastics in China Marine Waters

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中国海洋大学学报:英文版 2023年第5期22卷 1326-1340页

作者： GAO Fenglei LI Jingxi HU Jun LI Xianguo sun chengjun Key Laboratory of Marine Eco-Environmental Science and Technology Marine Bioresource and Environment Research CenterFirst Institute of OceanographyMinistry of Natural ResourcesQingdao 266061China Key Laboratory of Marine Chemistry Theory and Technology Ministry of EducationOcean University of ChinaQingdao 266100China Research Vessel Operation Center First Institute of OceanographyMinistry of Natural ResourcesQingdao 266061China Laboratory of Marine Drugs and Bioproducts Pilot National Laboratory for Marine Science and TechnologyQingdao 266071China

Microplastics(<5 mm)are ubiquitous in the environment and can pose potential danger to the ecosystem and even human *** the sink of microplastics,the ocean,especially the densely populated coastal area,has become a hotspot for research on microplastic *** the last decade,the research of marine microplastics has been rapidly increasing in *** review summarized the microplastic research conducted in China marine waters so far,and introduced the trends and progress of microplastic research in the four seas along the coast of *** reviewed and compared the current sampling,extraction,and identification methodologies of China's microplastic *** to the sampling method,the 30 reviewed studies were separated into two categories,trawl sampling and bulk sampling,to summarize relevant data,including abundance,sizes,shapes,colors and polymer types of *** main results showed that the distribution of microplastics in China's marine environment varied significantly,with offshore mariculture zones and the South China Sea being the most contaminated ***,granules(or pellets)and fibers were the most dominant microplastic colors and shapes,and the size of microplastics was influenced significantly by the sampling ***(PE),polypropylene(PP)and polystyrene(PS)were the most common polymer types found in the China Sea,accounting for 49.96%,29.97%,and 12.38%of the total studies,*** with other global data,China's coastal microplastic pollution is at an intermediate level and does not seem to be a major microplastic pollution source.

关键词： microplastics seawater trawl sampling bulk sampling China

Distribution pattern and geochemical analysis of rare earth elements in deep-ocean sediments

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Journal of Oceanology and Limnology 2021年第1期39卷 79-88页

作者： Jingxi LI chengjun sun Fenghua JIANG Fenglei GAO Yifan ZHENG Key Laboratory of Marine Eco-environmental Science and Technology Marine Bioresource and Environment Research CenterFirst Institute of OceanographyMinistry of Natural ResourcesQingdao 266061China Laboratory of Marine Drugs and Bioproducts Qingdao National Laboratory for Marine Science and TechnologyQingdao 266071China

The content and distribution pattern of rare earth elements(REEs)in surface sediments from the Eastern and Western Pacifi c Ocean,the Northern and Southern Atlantic Ocean,and the Southwestern Indian Ocean were explored and the resources and geochemical characteristics of REEs in deep-ocean sediments from diff erent oceans were *** total REE abundances(ΣREE)in the diff erent oceans ranged as follows:Eastern Pacifi c,56.88–500.02μg/g;Western Pacifi c,290.68–439.94μg/g;Northern Atlantic,55.33–154.90μg/g;Southern Atlantic,40.83–69.30μg/g;and Southwestern Indian Ocean,20.24–64.76μg/*** corresponding LREE(La-Eu)/HREE(Gd-Lu)average values were 5.18,5.86,9.01,5.21,and 4.59,which indicated that the light REEs were all evidently enriched.δEu andδCe showed slight Eu-negative anomalies and signifi cant Ce-positive anomalies in all *** the contents of REEs in the sediments varied among the diff erent oceans,the distribution patterns of REEs were similar,and the correlation coeffi cient was greater than *** the Eastern Pacifi c sediments,ΣREE showed a signifi cantly positive correlation with Co,Cu,Zn,Mn,Mo and a weak correlation with *** the Western Pacifi c and Southern Atlantic sediments,ΣREE presented no obvious correlation and a weakly negative correlation with Co,Cu,Zn,Mn,Mo and Fe,respectively.ΣREE in the Southwestern Indian Ocean sediments positively correlated with Cu,Zn,Mn,Mo,Fe,and had a weakly negative correlation with Co.

关键词： rare earth elements(REE)geochemistry distribution pattern REE fractionation marine sediment