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Atg24-mediated Mitophagy is Required for Host Invasion by the Plant Pathogenic Oomycete Peronophythora litchii

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Atg24-mediated Mitophagy is Required for Host Invasion by th...

中国植物病理学会2023年学术年会

作者： ZHANG Xue yang chengdong YU Ge LV Lin CHEN Qinghe

Peronophythora litchii is an oomycete pathogen that causes litchi downy blight, which is a major disease of litchi and leads to huge yield *** is a cellular process to selectively remove mitochondria through ***-related gene ATG24 has been reported to be involved in the process of mitophagy, and played an essential role in the pathogenic process in filamentous fungi,but its function in oomycetes is still ***, we identified a homologous protein of yeast Atg24 in *** according to sequence alignment, named *** used CRISPR/Cas9 system and PEGmediated protoplast transformation to knock out PlATG24 to know its functions in *** results showed that the number of sporangium and oospore, zoospore release rate, and pathogenicity of the PIATG24 mutants were significantly decreased than the wild type strain, but there was no significant difference in colony growth *** addition, the sporangium cleavage rate of the PlATG24 deletion mutants is also reduced compared with the wild type by FM4-64 staining, we therefore speculated that this was probably the main reason for the lower zoospores release rate in PlATG24 ***, we found that the deletion of PlATG24 interfered with the mitophagy process of *** by mito-tracker ***, these results of this study can provide some theoretical basis and new ideas for exploring the pathogenic mechanism ***.

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杉木人工林根际土壤性质变化的研究

林业科学 1999年第6期35卷 2-9页

作者：杨承栋焦如珍中国林业科学研究院林业研究所北京100091

本文重点论述一代杉木人工林根际和非根际土壤生物活性、生物化学活性、土壤化学性质的差异及其在林木生长过程中的动态变化规律。研究结果表明：杉木幼龄林、中龄林及近熟林样地土壤中有机养分和无机养分含量，除去幼龄林土壤中全Ｋ ... 详细信息

本文重点论述一代杉木人工林根际和非根际土壤生物活性、生物化学活性、土壤化学性质的差异及其在林木生长过程中的动态变化规律。研究结果表明：杉木幼龄林、中龄林及近熟林样地土壤中有机养分和无机养分含量，除去幼龄林土壤中全Ｋ之外，均是根际土壤养分含量高于非根际土壤。不同发育阶段养分变化规律：速效Ｎ在根际和非根际均呈下降趋势；速效Ｐ含量也呈下降趋势，仅从中龄林至近熟林略有恢复，但全Ｐ含量一直呈下降趋势，速效Ｋ由幼龄林至中龄林呈升高趋势，但由中龄林至近熟林呈下降趋势，全Ｋ只是从中龄林至近熟林呈下降趋势。Ｃｕ、Ｚｎ、Ｍｎ含量，从幼龄林至中龄林呈大幅度下降，中龄林至近熟林在非根际土壤中略有恢复。关于土壤酶活性变化，无论是水解酶类、还是氧化还原酶类活性，在杉木幼龄林时期，均是根际土壤酶活性低于非根际土壤，随着林木生长至近熟林，则逐渐出现根际土壤酶活性高于非根际土壤。土壤微生物区系研究结果表明：在幼龄林阶段根际土壤细菌、放线菌和真菌均多于非根际土壤，从幼龄林至中龄林根际土壤和非根际土壤细菌和真菌均呈下降趋势，但放线菌数量在增多，由中龄林至近熟林，细菌、真菌和放线菌在根际和非根际土壤中数量趋于提高。在杉木生长过程中。

关键词：杉木人工林根际土壤非根际土壤土壤性质

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智慧城轨自动售检票系统的技术发展趋势

都市快轨交通 2021年第1期34卷 52-56页

作者：杨承东刘洋中铁第四勘察设计院集团有限公司武汉430063

针对自动售检票(AFC)系统建设存在标准各异、互通性差等问题,通过分析新形势下的AFC系统需求,从精简化系统架构、交易数据实时上传、票卡虚拟化、支付多元化、安检票检一体化、乘车实名制化、互联互通等多个方面,提出AFC系统的技术发展... 详细信息

针对自动售检票(AFC)系统建设存在标准各异、互通性差等问题,通过分析新形势下的AFC系统需求,从精简化系统架构、交易数据实时上传、票卡虚拟化、支付多元化、安检票检一体化、乘车实名制化、互联互通等多个方面,提出AFC系统的技术发展趋势,为智慧城轨AFC系统的发展、建设和运营提供参考。

关键词：智慧城轨 AFC系统架构安检票检一体化实名制数字货币

Green intelligent fertilizers——A novel approach for aligning sustainable agriculture with green development

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Frontiers of Agricultural Science and Engineering 2024年第1期11卷 186-196页

作者： Maoying WANG Lingyun CHENG chengdong HUANG yang LYU Lin ZHANG Zhenya LU Changzhou WEI Wenqi MA Zed RENGEL Jianbo SHEN Fusuo ZHANG State Key Laboratory of Nutrient Use and Management College of Resources and Environmental SciencesNational Academy of Agriculture Green DevelopmentKey Laboratory of Plant–Soil Interactions(Ministry of Education)China Agricultural UniversityBeijing 100193China College of Agronomy Shihezi UniversityShihezi 832000China College of Resources and Environmental Science Hebei Agricultural UniversityBaoding 071000China Soil Science and Plant Nutrition UWA School of Agriculture and EnvironmentThe University of Western AustraliaPerthWA 6009Australia Institute for Adriatic Crops and Karst Reclamation Split 21000Croatia

This review addresses the growing disparity between the current state of fertilizer production in China and the evolving demands of green agriculture in the 21st century. It explores major advances in fertilizers, proposes the concept of green intelligent fertilizers and develops new strategies aligned with the principles of green development in fertilizer industry and *** intelligent fertilizers may be designed to maximize the synergistic effects among plants, soils, microorganisms, nutrient sources and the environment. This concept emphasizes the integration of industry and agriculture toward green development for entire industry chain, using an interdisciplinary approach to drive the green transformation of fertilizer industry, and promote green and sustainable development of agriculture. By bridging the gap between the current state of fertilizer industry and a growing need for environmentally responsible agricultural practices, this review highlights a path toward harmonizing fertilizer production with the imperatives of green agriculture.

关键词： Root-nutrient feedback green development intelligent fertilizers new fertilizer plant-soil-fertilizer interaction rhizosphere engineering

Small GTPase FgRabl is required for the vegetative growth,DON production and pathogenicity in Fusarium graminearum

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Small GTPase FgRabl is required for the vegetative growth,DO...

中国植物病理学会2021年学术年会

作者： YUAN Yanping LI Jingjing yang chengdong CHEN Xin ZHENG Huawei ZHOU Jie College of Life Sciences Fujian Agriculture and Forestry University Marine and Agricultural Biotechnology Laboratory Institute of OceanographyMinjiang University

Fusarium graminearum is the major pathogen causing Fusarium head blight（FHB）,which not only severely reduces crop yields,but also contaminates grains with mycotoxins such as deoxynivalenol（DON）,that can seriously threaten to human and animal *** GTPases are key regulators of intracellular vesicle transport,and they modulate the development and pathogenicity of plant pathogens through mediated endocytosis and secretion ***,the biological function of FgRabl is still unclear in *** this study,we constructed the constitutively active（CA） and dominant negative（DN） vectors of FgRABl and transformed them into the wild type *** analyses of CA and DN transformants showed that FgRABl is important for the vegetative growth,cell wall formation and mycelial tip *** to wild type PH-1,the conidiation of Fgrab1 DN transformants was significantly reduced with abnormal conidial ***1 DN was significantly reduced pathogenicity in wheat *** localization analysis showed that FgRabl localized to the Golgi apparatus,endoplasmic reticulum and *** further found that the DON content in the mycelia and culture medium of the FgRab1 DN transformant was both significantly reduced compared with that of the wild type *** analyses demonstrated that the inactivation of FgRab1 impairs the production and secretion of DON by affecting the expression of DON biosynthesis genes and toxin transporter,as well as the localization of toxin ***,FgRabl was found to be crucial for FgSnc1-mediated fusion of secretory vesicles from Golgi to plasma membrane in *** summary,our results indicate that FgRab1 plays vital roles in the vegetative growth,conidiogenesis,DON production,and the pathogenicity of ***.

关键词： Fusarium graminearum FgRab1 dominant negative vesicle transport DON

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综合属性选择和删除的属性约简方法

智能系统学报 2013年第2期8卷 183-186页

作者：杨成东邓廷权临沂大学信息学院山东临沂276005 哈尔滨工程大学理学院黑龙江哈尔滨150001

属性约简能有效地消除信息冗余,广泛应用于人工智能、机器学习.通过实例指出基于辨识矩阵的经典的属性约简方法存在不能得到约简的可能性,仍具有冗余性.因此,提出了综合属性选择和删除算法的辨识矩阵属性约简方法,并有效解决该问题.通过... 详细信息

属性约简能有效地消除信息冗余,广泛应用于人工智能、机器学习.通过实例指出基于辨识矩阵的经典的属性约简方法存在不能得到约简的可能性,仍具有冗余性.因此,提出了综合属性选择和删除算法的辨识矩阵属性约简方法,并有效解决该问题.通过UCI标准数据集验证表明,新方法比经典方法进一步减少了属性的个数,凸显其实用性和有效性.

关键词：辨识矩阵属性约简信息冗余人工智能机器学习属性选择属性删除

Research on Change of Rhizosphere Soil Properties of Chinese fir Plantation

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Chinese Forestry Science and Technology 2002年第4期1卷 18-26页

作者： yang chengdong JIAO Ruzhen SUN QiwuResearch Institute of Forestry. Chinese Academy of Forestry. Beijing 100091. China

This article emphatically reviews the difference of soil biological activities, biochemical activities and soil chemical properties between the rhizosphere and non-rhizosphere soil of first rotation of Chinese fir (Cunninghamia lanceolata (Lamb) Hook) plantation. It also reviews their dynamic patterns during Chinese fir plantation development. The results show that the contents of organic and inorganic nutrients in the rhizosphere soil of young, half-mature and near-mature Chinese fir of first-rotation ...

关键词： Chinese fir plantation rhizosphere soil non-rhizosphere soil soil properties

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电液比例阀综合性能试验台的研制

机床与液压 2017年第22期45卷 172-175页

作者：谢鲲杨成东上海应用技术大学轨道交通学院上海201418 旌希数控技术(上海)有限公司上海201400

根据电液比例阀的技术要求和检测方法,研制电液比例阀综合性能试验台,介绍该试验台控制系统及液压系统的组成,尤其是对电控驱动信号部分进行了改进设计,叠加了颤振信号。测试结果表明:该试验台测试精度高、性能稳定、拆装快速准确、测... 详细信息

根据电液比例阀的技术要求和检测方法,研制电液比例阀综合性能试验台,介绍该试验台控制系统及液压系统的组成,尤其是对电控驱动信号部分进行了改进设计,叠加了颤振信号。测试结果表明:该试验台测试精度高、性能稳定、拆装快速准确、测试时间短、测试结果可视化程度高、历史数据查询方便,可为电液比例阀的研制和在线检测提供可靠的测试依据和试验手段。

关键词：电液比例阀液压试验台脉宽调制

PlAtg12 is Involved in the Vegetative Growth,Development and Pathogenicity of Peronophythora litchii

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PlAtg12 is Involved in the Vegetative Growth,Development and...