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Mutual regulation of microglia and astrocytes after Gas6 inhibits spinal cord injury

Neural Regeneration Research 2025年第2期20卷 557-573页

作者： Jiewen chen Xiaolin Zeng Le Wang Wenwu Zhang Gang Li Xing cheng Peiqiang Su Yong Wan Xiang Li Department of Spine Surgery The First Affiliated HospitalSun Yat-sen UniversityGuangzhouGuangdong ProvinceChina Guangdong Province Key Laboratory of Orthopedics and Traumatology GuangzhouGuangdong ProvinceChina

Invasive inflammation and excessive scar formation are the main reasons for the difficulty in repairing nervous tissue after spinal cord *** and astrocytes play key roles in the spinal cord injury micro-environment and share a close ***,the mechanisms involved remain *** this study,we found that after spinal cord injury,resting microglia(M0)were polarized into pro-inflammatory phenotypes(MG1 and MG3),while resting astrocytes were polarized into reactive and scar-forming *** expression of growth arrest-specific 6(Gas6)and its receptor Axl were significantly down-regulated in microglia and astrocytes after spinal cord *** vitro experiments showed that Gas6 had negative effects on the polarization of reactive astrocytes and pro-inflammatory microglia,and even inhibited the cross-regulation between *** further demonstrated that Gas6 can inhibit the polarization of reactive astrocytes by suppressing the activation of the Yes-associated protein signaling ***,in turn,inhibited the polarization of pro-inflammatory microglia by suppressing the activation of the nuclear factor-κB/p65 and Janus kinase/signal transducer and activator of transcription signaling *** vivo experiments showed that Gas6 inhibited the polarization of pro-inflammatory microglia and reactive astrocytes in the injured spinal cord,thereby promoting tissue repair and motor function ***,Gas6 may play a role in the treatment of spinal cord *** can inhibit the inflammatory pathway of microglia and polarization of astrocytes,attenuate the interaction between microglia and astrocytes in the inflammatory microenvironment,and thereby alleviate local inflammation and reduce scar formation in the spinal cord.

关键词： astrocytes Axl cell polarization Gas6 Hippo signal inflammatory micro-environment intercellular interaction microglia single-cell sequencing spinal cord injury

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Glutamatergic CYLD deletion leads to aberrant excitatory activity in the basolateral amygdala:association with enhanced cued fear expression

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Neural Regeneration Research 2025年第11期20卷 3259-3272页

作者： Huidong Li Faqin Li Zhaoyi chen Erwen Wu Xiaoxi Dai Danni Li Haojie An Shiyi Zeng Chunyan Wang Li Yang cheng Long School of Life Sciences South China Normal UniversityGuangzhouGuangdong ProvinceChina School of Life Sciences Guangzhou UniversityGuangzhouGuangdong ProvinceChina Guangdong Provincial Key Laboratory of Major Obstetric Diseases The Third Affiliated Hospital of Guangzhou Medical UniversityGuangzhouGuangdong ProvinceChina South China Normal University-Panyu Central Hospital Joint Laboratory of Translational Medical Research Panyu Central HospitalGuangzhouGuangdong ProvinceChina

Neuronal activity,synaptic transmission,and molecular changes in the basolateral amygdala play critical roles in fear ***(CYLD)is a deubiquitinase that negatively regulates the nuclear factor kappa-B *** is well studied in non-neuronal cells,yet underinvestigated in the brain,where it is highly *** studies have shown involvement of CYLD in the remodeling of glutamatergic synapses,neuroinflammation,fear memory,and anxiety-and autism-like ***,the precise role of CYLD in glutamatergic neurons is largely ***,we first proposed involvement of CYLD in cued fear *** next constructed transgenic model mice with specific deletion of Cyld from glutamatergic *** results show that glutamatergic CYLD deficiency exaggerated the expression of cued fear in only male ***,loss of CYLD in glutamatergic neurons resulted in enhanced neuronal activation,impaired excitatory synaptic transmission,and altered levels of glutamate receptors accompanied by over-activation of microglia in the basolateral amygdala of male ***,our study suggests a critical role of glutamatergic CYLD in maintaining normal neuronal,synaptic,and microglial *** may contribute,at least in part,to cued fear expression.

关键词： basolateral amygdala cued fear expression cylindromatosis deubiquitinase glutamate receptor 1 glutamatergic neuron microglial activation N-methyl-D-aspartate receptor 1 neuronal activation synaptic transmission

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Cerebral fat embolism following autologous fat injection in facial reconstruction:A case report

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World Journal of Clinical Cases 2025年第2期13卷 50-58页

作者： Xiu-Ying chen Fa Shen Chang cheng Yu-Han Wang Wen-Chao cheng De-Zhi Yuan Wen Huang Department of Neurology Chongqing Emergency Medical CenterChongqing University Central HospitalChongqing 400000China Department of Neurology Xinqiao HospitalThe Army Medical University(Third Military Medical University)Chongqing 400000China

BACKGROUND Autologous fat injection in facial reconstruction is a common cosmetic *** cerebral fat embolism(CFE)as a complication is rare,it carries serious health *** SUMMARY We present a case of a 29-year-old female patient who developed acute CFE following facial fat filling *** the surgery,the patient experienced symptoms including headache,nausea,vomiting,and difficulty breathing,which was followed by neurological symptoms such as slurred speech and left-sided *** physical examination and auxiliary investigations,including blood tests,head and neck computed tomography angiography,and cranial magnetic resonance diffusion-weighted imaging,were performed upon *** clinical diagnosis was acute cerebral embolism following facial fat filling *** included measures to improve cerebral circulation,dehydration for intracranial pressure reduction,nutritional support,and rehabilitation therapy for left limb *** patient showed a significant improvement in symptoms after 2 weeks of *** recovered left limb muscle strength to grade 5,had clear speech,and experienced complete relief of *** Our case highlights the potential occurrence of severe complications in patients undergoing fat injection in facial *** prevent these complications,plastic surgeons should enhance their professional knowledge and skills.

关键词： Cerebral fat embolism Mechanical thrombectomy Thrombolysis Steroid therapy Symptomatic treatment Case report

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T cell interactions with microglia in immune-inflammatory processes of ischemic stroke

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Neural Regeneration Research 2025年第5期20卷 1277-1292页

作者： Yuxiao Zheng Zilin Ren Ying Liu Juntang Yan Congai chen Yanhui He Yuyu Shi Fafeng cheng Qingguo Wang Changxiang Li Xueqian Wang School of Traditional Chinese Medicine Beijing University of Chinese MedicineBeijingChina Library Beijing University of Chinese MedicineBeijingChina Dongzhimen Hospital Beijing University of Chinese MedicineBeijingChina

The primary mechanism of secondary injury after cerebral ischemia may be the brain inflammation that emerges after an ischemic stroke,which promotes neuronal death and inhibits nerve tissue *** the first immune cells to be activated after an ischemic stroke,microglia play an important immunomodulatory role in the progression of the *** an ischemic stroke,peripheral blood immune cells(mainly T cells)are recruited to the central nervous system by chemokines secreted by immune cells in the brain,where they interact with central nervous system cells(mainly microglia)to trigger a secondary neuroimmune *** review summarizes the interactions between T cells and microglia in the immune-inflammatory processes of ischemic *** found that,during ischemic stroke,T cells and microglia demonstrate a more pronounced synergistic ***1,Th17,and M1 microglia can co-secrete proinflammatory factors,such as interferon-γ,tumor necrosis factor-α,and interleukin-1β,to promote neuroinflammation and exacerbate brain ***2,Treg,and M2 microglia jointly secrete anti-inflammatory factors,such as interleukin-4,interleukin-10,and transforming growth factor-β,to inhibit the progression of neuroinflammation,as well as growth factors such as brain-derived neurotrophic factor to promote nerve regeneration and repair brain *** interactions between microglia and T cells influence the direction of the subsequent neuroinflammation,which in turn determines the prognosis of ischemic stroke *** trials have been conducted on the ways to modulate the interactions between T cells and microglia toward anti-inflammatory communication using the immunosuppressant fingolimod or overdosing with Treg cells to promote neural tissue repair and reduce the damage caused by ischemic ***,such studies have been relatively infrequent,and clinical experience is still *** summary,in ischemic stroke,T cell subsets and activated micro

关键词： brain immune inflammation interaction ischemic stroke mechanism microglia neuron secondary injury T cells

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Human-induced pluripotent stem cell-derived neural stem cell exosomes improve blood-brain barrier function after intracerebral hemorrhage by activating astrocytes via PI3K/AKT/MCP-1 axis

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Neural Regeneration Research 2025年第2期20卷 518-532页

作者： Conglin Wang Fangyuan cheng Zhaoli Han Bo Yan Pan Liao Zhenyu Yin Xintong Ge Dai Li Rongrong Zhong Qiang Liu Fanglian chen ping Lei Department of Geriatrics Tianjin Medical University General HospitalTianjinChina School of Medicine Nankai UniversityTianjinChina Tianjin Neurological Institute TianjinChina

Cerebral edema caused by blood-brain barrier injury after intracerebral hemorrhage is an important factor leading to poor ***-induced pluripotent stem cell-derived neural stem cell exosomes(hiPSC-NSC-Exos)have shown potential for brain injury repair in central nervous system *** this study,we explored the impact of hiPSC-NSC-Exos on blood-brain barrier preservation and the underlying *** results indicated that intranasal delivery of hiPSC-NSC-Exos mitigated neurological deficits,enhanced blood-brain barrier integrity,and reduced leukocyte infiltration in a mouse model of intracerebral ***,hiPSC-NSC-Exos decreased immune cell infiltration,activated astrocytes,and decreased the secretion of inflammatory cytokines like monocyte chemoattractant protein-1,macrophage inflammatory protein-1α,and tumor necrosis factor-αpost-intracerebral hemorrhage,thereby improving the inflammatory *** sequencing indicated that hiPSC-NSC-Exo activated the PI3K/AKT signaling pathway in astrocytes and decreased monocyte chemoattractant protein-1 secretion,thereby improving blood-brain barrier *** with the PI3K/AKT inhibitor LY294002 or the monocyte chemoattractant protein-1 neutralizing agent C1142 abolished these *** summary,our findings suggest that hiPSC-NSC-Exos maintains blood-brain barrier integrity,in part by downregulating monocyte chemoattractant protein-1 secretion through activation of the PI3K/AKT signaling pathway in astrocytes.

关键词： AKT astrocyte blood-brain barrier cerebral edema exosomes human-induced pluripotent stem cells intracerebral hemorrhage neural stem cells neuroinflammation PI3K

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Optimization of Two-Dimensional Gel Electrophoresis for Kenaf Leaf Proteins

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Agricultural Sciences in China 2011年第12期10卷 1842-1850页

作者： chen Tao QI Jian-min XU Jian-tang chen pin-pin TAO Ai-fen chen Fu-cheng chen Wei Key Laboratory for Genetics Breeding and Multiple Utilization of Crops Ministry of Education/Fujian Agriculture and Forestry University Fuzhou 350002. P.R.China

To establish a suitable and effective protocol of protein extraction for two-dimensional gel electrophoresis （2-DE） analysis in kenaf leaf tissues, three extraction methods （trichloroacetic acid/acetone, urea/thiourea, and phenol extraction methods） were applied to the extraction of kenaf leaf protein. The results were compared in regard to protein extraction efficiency, sodiumdodecyl sulfate-polyacrylamide gel electrophoresis （SDS-PAGE）, and 2-DE gels. Furthermore, the 2-DE system was optimized for four aspects： the pH range of IPG （immobilized pH gradient） stripes, sampling methods, sample volumes, and concentration of polyacrylamide gels. The data presented showed that the phenol extraction method is the best method to perform 2-DE analysis of kenaf leaf protein. The protein extracted from phenol extraction method reached the purity of （26.40±0.859）%, showed （25.67±1.53） protein bands in one dimension SDS-PAGE gels, and （1 374±54.44） protein spots on 2-DE gels. The research also indicates that kenaf leaf protein spots were distributed mainly within the pH range of 4-8. More clear background with a better distribution effect and many protein spots could be obtained on 2-DE gels under the conditions of active rehydration loading, 24 cm IPG strips （linear pH gradient of 4-7）, 1.4 mg samples, and 12% SDS-PAGE gels.

关键词： kenaf protein extraction proteome two-dimensional gel electrophoresis

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Fundamental modes in waveguide pipe twisted by saturated double-well potential

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Frontiers of physics 2017年第1期12卷 107-116页

作者： Gui-Hua chen Hong-cheng Wang Zhao-pin chen Yan Liu Department of Electronic Engineering Dongguan University of Technology Dongguan 523808 China Department of Physical Electronics School of Electrical Engineering Faculty of Engineering Tel Aviv University Tel Aviv IL-69978 Israel Department of Applied Physics South China Agricultural University Guangzhou 510642 China

We study fundamental modes trapped in a rotating ring with a saturated nonlinear double-well potential. This model, which is based on the nonlinear Schrodinger equation, can be constructed in a twisted waveguide pipe in terms of light propagation, or in a Bose-Einstein condensate （BEC） loaded into a toroidal trap under a combination of a rotating π-out-of-phase linear potential and nonlinear pseudopotential induced by means of a rotating optical field and the Feshbach resonance. Three types of fundamental modes are identified in this model, one symmetric and the other two asymmetric. The shape and stability of the modes and the transitions between different modes are investigated in the first rotational Brillouin zone. A similar model used a Kerr medium to build its nonlinear potential, but we replace it with a saturated nonlinear medium. The model exhibits not only symmetry breaking, but also symmetry recovery. A specific type of unstable asymmetric mode is also found, and the evolution of the unstable asymmetric mode features Josephson oscillation between two linear wells. By considering the model as a configuration of a BEC system, the ground state mode is identified among these three types, which characterize a specific distribution of the BEC atoms around the trap.

关键词： Twisted double-well waveguide saturated nonlinear potential symmetry breaking symmetry recovery

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Prediction of electric-powered fixed-wing UAV endurance

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航空动力学报 2017年第9期32卷 2170-2179页

作者： cheng Feng WANG Hua CUI pin School of Astronautics Beijing University of Aeronautics and Astronautics Science and Technology on Space Physics Laboratory China Academy of Launch Vehicle Technology

To overcome the problems encountered in predicting the endurance of electricpowered fixed-wing unmanned aerial vehicles(UAVs),which were stemmed from the dynamic changes in electric power system efficiency and battery discharge characteristics under different operating conditions,the required battery power model and battery discharge model were *** required battery power model was determined using an approximate model of electric power system efficiency based on wind tunnel testing and the self-adaptive penalty ***,current correction and ambient temperature correction terms were proposed for the trained Kriging model representing the discharge characteristics under standard operation,and then the discharged capacity-terminal voltage model was *** numerical integration of this model with the required battery power model,the electric-powered fixed-wing UAV endurance prediction model was *** tests indicated that the proposed endurance model could precisely calculate the battery discharge time and accurately describe the battery discharge *** similarity of the theoretical and flight test results reflected the accuracy of the proposed endurance model as well as the importance of considering dynamic changes in power system efficiency in endurance *** proposed endurance model meeting precision requirements can be used in practical engineering applications.

关键词： fixed-wing unmanned aerial vehicle(UAV) endurance Peukert equation self-adaptive penalty function power system efficiency

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Rotorcraft flight endurance estimation based on a new battery discharge model

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Chinese Journal of Aeronautics 2017年第4期30卷 1561-1569页

作者： Feng cheng Hua WANG pin CUI School of Astronautics Beihang University Beijing 100083 China Science and Technology on Space Physics Laboratory Beijing 100076 China

To avoid the numerical complexities of the battery discharge law of electric-powered rotorcrafts,this study uses the Kriging method to model the discharge characteristics of Li-Po batteries under standard conditions.A linear current compensation term and an ambient temperature compensation term based on radial basis functions are then applied to the trained Kriging model,leading to the complete discharged capacity-terminal voltage *** an orthogonal experimental design and a sequential method,the coefficients of the current and ambient temperature compensation terms are determined through robust *** endurance calculation model for electric-powered rotorcrafts is then established,based on the battery discharge model,through numerical *** tests show that the maximum relative error of the proposed discharged capacity-terminal voltage model at detection points is 0.0086,and that of the rotorcraft endurance calculation model is 0.0195,thus verifying their accuracy.A flight test further demonstrates the applicability of the proposed endurance model to general electric-powered rotorcrafts.

关键词： Kriging model Orthogonal experimental design Robust optimization Rotorcraft endurance Sequential method

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Matched glass-to-Kovar seals in N_2 and Ar atmospheres

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International Journal of Minerals,Metallurgy and Materials 2013年第9期20卷 874-882页

作者： cheng-hsien Kuo Pi-ying cheng Chang-pin Chou Department of Mechanical Engineering Chiao Tung University

The oxidation of Kovar alloy was investigated, the wetting and spreading behavior of hard and soft glasses on Kovar alloy were studed by using the sessile drop method, and the quality and the seal strength of glass-Kovar seals were tested. The experimental results indicated that the preoxidation of Kovar alloy for approximately 10 min at 700℃ in air resulted in excellent adherence in glass-Kovar seals. The wetting and spreading behavior of glass on preoxidized Kovar alloy were superior to that on nonoxidized Kovar alloy. The wetting ability of ASF110 glass, at 950℃ and 980℃ in Ar and N2 atmospheres, was significantly superior to that of ASF200R and ASF700 glasses. The seal quality of the glass-preoxidized Kovar seal was superior to that of the glass-nonoxidized Kovar seal. The shear strength of the ASFll0 glass-preoxidized Kovar seal, which was prepared at 980℃ for 20 min in an Ar atmosphere, was approximately 3.9 MPa.

关键词： Kovar alloy glass sealing argon nitrogen oxidation wetting

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