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Banking of perinatal mesenchymal stem/stromal cells for stem cellbased personalized medicine over lifetime:Matters arising

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World Journal of Stem Cells 2023年第4期15卷 105-119页

作者： cheng-hai li Jing Zhao Hong-Yan Zhang Bin Wang Stem Cell Program of Clinical Research Center People’s Hospital of Zhengzhou University and Henan Provincial People’s HospitalZhengzhou 450003Henan ProvinceChina Department of Clinical Laboratory People’s Hospital of Zhengzhou University and Henan Provincial People’s HospitalZhengzhou 450003Henan ProvinceChina Department of Pharmacy Fuwai Central China Cardiovascular HospitalZhengzhou 450000Henan ProvinceChina Department of Neurosurgery People’s Hospital of Zhengzhou University and Henan Provincial People’s HospitalZhengzhou 450003Henan ProvinceChina

Mesenchymal stromal/stem cells(MSCs)are currently applied in regenerative medicine and tissue *** clinical studies have indicated that MSCs from different tissue sources can provide therapeutic benefits for *** derived from either human adult or perinatal tissues have their own unique advantages in their medical ***,clinical studies are conducted by using of cultured MSCs after thawing or short-term cryopreserved-then-thawed MSCs prior to administration for the treatment of a wide range of diseases and medical ***,cryogenically banking perinatal MSCs for potential personalized medicine for later use in lifetime has raised growing interest in China as well as in many other ***,this has led to questions regarding the availability,stability,consistency,multipotency,and therapeutic efficiency of the potential perinatal MSC-derived therapeutic products after longterm *** opinion review does not minimize any therapeutic benefit of perinatal MSCs in many diseases after short-term *** article mainly describes what is known about banking perinatal MSCs in China and,importantly,it is to recognize the limitation and uncertainty of the perinatal MSCs stored in cryobanks for stem cell medical treatments in whole *** article also provides several recommendations for banking of perinatal MSCs for potentially future personalized medicine,albeit it is impossible to anticipate whether the donor will benefit from banked MSCs during her/his lifetime.

关键词： Mesenchymal stromal/stem cells Adult mesenchymal stromal/stem cells Perinatal mesenchymal stromal/stem cells Perinatal tissue Stem cell bank Personalized medicine

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Synthesis and Herbicidal Activity of Novel Sulfonylureas Containing Thiadiazol Moiety

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Chemical Research in Chinese Universities 2008年第1期24卷 32-35页

作者： GUO Wan-cheng liU Xing-hai li Yong-hong WANG Su-hua li Zheng-ming Institute of Elemento-Organic Chemistry State Key Laboratory of Elemento-Organic Chemistry Nankai University Tianjin 300071 P. R. China

Thirteen novel sulfonylureas containing thiadiazole moiety were synthesized in a two-step reaction. Their structures were determined using IR, ^1H NMR, HRFTMS, and elemental analysis. Herbicidal activities of these compounds were determined in the green house bio-assay. The results show that four compounds among them exhibit some activity toward four tested herbs.

关键词： Sulfonylurea Synthesis Herbicidal activity

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liSR28 Is Involved in Pollen Germination by Affecting Filamentous Actin Dynamics

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Molecular Plant 2013年第4期6卷 1163-1175页

作者： li-Juan Cao Meng-Meng Zhao Chang liu Huai-Jian Dong Wang-cheng li hai-Yun Ren Key Laboratory of Cell Proliferation and Regulation Biology of Ministry of Education College of Life Science Beijing Normal University Beijing 100875China

Alternative splicing plays important roles in gene regulation and contributes to protein complexity. Previous studies suggest that alternative splicing exists in members of the villin/gelsolin/fragmin superfamily. In this study, a ser- ine/argine-rich （SR） protein cDNA with 28kDa protein （liSR28） was isolated from a lily （lilium Iongiflorum） expression library. Protein domain analysis showed that liSR28 had similar structures to Arabidopsis SR45 （AtSR45）, and liSR28 could complement the phenotype of loss of AtSR45 function. Therefore, overexpression of liSR28 and AtSR45 mutant （atsr45-1） were used in the following experiments. Overexpression of liSR28 in Arabidopsis completely inhibited pollen germina- tion. In contrast, the pollen germination of atsr45-1 was earlier than that of wild-type. In addition, pollen of atsr45-1 contained less F-actin at the corresponding hydration stage during pollen germination compared to that of wild-type. Alternative splicing analysis showed that Arabidopsis villinl （AtVLN1） transcript encoding the full-length protein was increased, and that encoding the truncated protein was decreased in atst45-1. Moreover, the mRNA expression level of other actin-binding proteins （ABPs） abundant in Arabidopsis pollen was also changed in atsr45-1. In conclusion, we hypothesize that liSR28 alters F-actin dynamics probably through its alternative splicing activities to affect directly or indirectly the alternative splicing of AtVLN1 and the expression of different ABPs, which then affects the pollen germination.

关键词： SR protein alternative splicing F-actin.

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On Network Security Situation Prediction Based on RBF Neural Network 36

On Network Security Situation Prediction Based on RBF Neural...

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第36届中国控制会议

作者： Yang Jiang cheng-hai li li-shan Yu Bo Bao College of Air and Missile Defense Air Force Engineering University

ISBN: (纸本)9781538629185

Aiming at the problem of network security situation prediction,this paper studies the prediction method based on RBF neural *** training the RBF neural network,find out the nonlinear mapping relationship between the front N data and the subsequent Mdata,and then adjust the value of N to explore the different prediction *** simulation result shows that the proposed method can accurately predict the results of the *** with the prediction based on BP neural network,the proposed method has a more accurate prediction and a faster convergence speed,a better prediction effect achieved.

关键词： Network security Situation prediction RBF

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Synthesis of a novel tri-antennary galactoside with high hepatocyte targeting

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Chinese Chemical Letters 2007年第5期18卷 513-515页

作者： Lei Tang Yong Wu Jiao Lu Zhi Rong Zhang Jin cheng Yang li hai Key Laboratory of Drug Targeting Education Ministry West China School of Pharmacy Sichuan University Chengdu 610041 PR China

A novel bifunctional compound carrying duster thiogalactoside as the cell targeting ligands was synthesized for gene delivery to hepatocytes. Tetra-antennary dendr-OMs4 5 was used as a scaffold for the attachment of three galactosides, while the other mesylate end was linked with cholesterol through poly（ethylene glycol） chain. This design provided an effective entry for the synthesis of the bifunctional compound.

关键词： Cluster Cholesterylated galactoside Targeting ligand Hepatocyte-specific delivery Gene delivery

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On Some Varieties of Soluble lie Algebras

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Communications in Mathematical Research 2012年第1期28卷 10-16页

作者： NAN JI-ZHU WANG cheng-cheng li hai-liNG School of Mathematical Sciences Dalian University of Technology Dalian Liaoning 116024

In this paper, we study a class of soluble lie algebras with variety relations that the commutator of m and n is zero. The aim of the paper is to consider the relationship between the lie algebra L with the variety relations and the lie algebra L which satisfies the permutation variety relations for the permutation ψ of {3, ··· , k}.

关键词： variety permutation nilpotent class

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A modified heuristics-based model for simulating realistic pedestrian movement behavior

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Chinese Physics B 2022年第9期31卷 362-370页

作者： Wei-li Wang hai-cheng li Jia-Yu Rong Qin-Qin Fan Xin Han Bei-Hua Cong Institute of Logistics Science and Engineering Shanghai Maritime UniversityShanghai 201306China Shanghai Institute of Disaster Prevention and Relief Tongji UniversityShanghai 200092China

Pedestrian movement simulation models are used in various areas,such as building evacuation,transportation engi-neering,and safety management of large *** also provides effective means to uncover underlying mechanisms of collective *** this work,a modified heuristics-based model is *** this model,the potential collisions in the moving process are explicitly ***,a series of simulations is conducted in two typical scenarios to demonstrate the influence of critical parameters on model *** is found that when facing a wide obstacle in a corridor,the larger the visual radius,the earlier the pedestrian starts to make a *** addition,when a pedestrian observes a large crowd walking toward him,he chooses to make a detour and moves in the flow in a uniform ***,the model can reproduce the lane formation pedestrian flow phenomena in relatively high-density *** the increase of pedestrian visual radius and the weight of potential collision resistance,more stable pedestrian lanes and fewer moving-through-the-counterflow pedestrians can be *** terms of model validation,the density-speed relationship of simulation results accords well with that of the published empirical *** results demonstrate that the modified heuristics-based model can overcome the deficiency of the original model,and reproduce more realistic pedestrian movement behavior.

关键词： heuristics-based model lane formation pedestrian flow potential collisions

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Study on the Intermediate in the o-Phenylenediamine Oxidative Reaction Using Hemoglobin as A Mimetic Peroxidase in Aqueous-Organic Two Phase

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Wuhan University Journal of Natural Sciences 2002年第1期7卷 117-121页

作者： li hai-cheng li De-jia Huang Bo Jin De-long Zou Guo-lin College of life Sciences Wuhan University Wuhan 430072 Hubei China

Hemoglobin was used as a mimetic enzyme for peroxidase to catalyze the oxidative reaction of o-phenylenediamine with H2O2 which functioned as an oxidant. The relationship between physicochemical properties of the intermediate and enzymatic activity of hemoglobin was studied. Since the solubility of the intermediate in the reaction is higher in butanol phase than in water phase, the intermediate itself diffused from the aqueous phase to the butanol phase. The experimental results showed that the rate of product and the stability of intermediate were associated with the temperature and the pH value of the buffer. The formation rate of intermediate and half-life period reveal the maximal in pH7, nevertheless, the whole rate of the catalytic reaction is the greatest in pH5, which the ratio of the initial rate in final product formation compared to that intermediate formation is the greatest.

关键词： hemoglobin mimetic enzyme intermediate two phase reaction mechanism

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Inhibition of connective tissue growth factor overexpression decreases growth of hepatocellular carcinoma cells in vitro and in vivo

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Chinese Medical Journal 2011年第22期124卷 3794-3799页

作者： JIA Xiao-qin cheng hai-qing li Hong ZHU Yan li Yu-hua FENG Zhen-qing ZHANG Jian-ping Department of Pathology School of Medicine Yangzhou University Yangzhou Jiangsu 225500 China

Background We have previously found that connective tissue growth factor （CTGF） is highly expressed in a rat model of liver cancer. Here, we examined expression of CTGF in human hepatocellular carcinoma （HCC） cells and its effect on celt growth. Methods Real-time PCR was used to observe expression of CTGF in human HCC cell lines HepG2, SMMC-7721, MHCC-97H and LO2. siRNA for the CTGFgene was designed, synthesized and cloned into a PIk0.1-GFP-SP6 vector to construct a lentivirus-mediated shRNA/CTGF. CTGF mRNA and protein expression in HepG2 cells treated by CTGF-specific shRNA was evaluated by real-time PCR and Western blotting. 3-（4,5-dimethylthiazol-2-yl）- 2,5-diphenyltetrazolium bromide （MTT） assay was utilized to evaluate the growth effect, and a colony formation assay was used for observing clonogenic growth. In vivo, tumor cell proliferation was evaluated in a nude mouse model of xenotransplantation. Statistical significance was determined by t test for comparison between two groups, or analysis of variance （ANOVA） for multiple groups. Results Immunohistochemical staining of CTGF was seen in 35 of 40 HCC samples （87.5%）. CTGF was overexpressed 5-fold in 20 HCC tissues, compared with surrounding non-tumor liver tissue. CTGF mRNA level was 5-8-fold higher in HepG2, SMMC-7721 and MHCC-97H than in LO2 cells. This indicated that the inhibition rate of cell growth was 43% after knockdown of CTGF expression （P 〈0.05）. Soft agar colony formation assay showed that siRNA mediated knockdown of CTGF inhibited colony formation in soft agar of HepG2 cells （P 〈0.05）. The volume of tumors from CTGF-shRNA-expressing cells only accounted for 35% of the tumors from the scrambled control-infected HepG2 cells （P 〈0.05）. Conclusions CTGF was overexpressed in human HCC cells and downregulation of CTGF inhibited HCC growth in vitro and in vivo. Knockdown of CTGFmay be a potential therapeutic strategy for treatment of HCC.

关键词： hepatocellular carcinoma connective tissue growth factor siRNA cell proliferation

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Comparison of the Developmental Potential and Clinical Results of In Vivo Matured Oocytes Cryopreserved with Different Vitrification Media

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Chinese Medical Journal 2015年第22期128卷 3029-3034页

作者： Mei li Miao-Miao Wang Hui liu Ke-liang Wu Shui-Ying Ma cheng li hai-Bin Zhao Zi-Jiang Chen Center for Reproductive Medicine Provincial Hospital Affiliated to Shandong University National Research Center for Assisted Reproductive Technology andReproductive Genetics Key Laboratory for Reproductive Endocrinology of Ministry of EducatLon Shandong Provincial Key Laboratory of Reproductive Medicine Jinan Shandong 250021 China

Background： Oocyte vitrification is widely used throughout the world, but its clinical efficacy is inconsistent and depends on the vitrification media. This study compared the developmental potential and clinical results of in vivo matured oocytes cryopreserved with different vitrification media. Methods： This retrospective study involved vitrified-warmed oocytes at one in vitro fertilization laboratory. Vitrification media kits comprised the MC kit （ethylene glycol （EG] plus 1,2-propanediol [PROH]）, the KT kit （EG plus dimethyl sulphoxide [DMSO]）, and the Modified kit （EG plus DMSO and PROH kit）. Rates of oocyte survival and subsequent developmental potential were recorded and analyzed. The t-test and the Chi-square test were used to evaluate each method＇s efficacy. Results： Oocyte survival rate was significantly higher for the Modified kit （92.0%） than for the MC kit （88.2%）（P 〈 0.05） and the KT kit （77.3%）（P 〈 0.001）. The rate of high-quality embryo development in the Modified kit group （35.8%） was significantly higher than in the MC kit group （29.0%） and the KT kit group （28.3%）（P 〈 0.001 ）. No significant differences were observed in the clinical pregnancy and implantation rates among the MC, KT, and Modified kit groups （37.2% vs. 30.2% vs. 39.6%; 21.9% vs. 18.8% vs. 27.4%, respectively）（P 〉 0.05）. The high-quality embryo rate per warmed oocyte was significantly higher （23.4%） in the Modified kit group than in the other groups （P 〈 0.001）. The embryo utilization and live birth rates per warmed oocyte were the highest in the Modified kit group, but not significantly （P 〉 0.05）. Conclusions： Modified vitrification media are efficient for oocyte vitrification and. with further verification, may be able to replace commercially available media in future clinical applications.

关键词： Fertilization live Birth Oocytes Pregnancy Vitrification Media

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