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Regulation of pepc gene expression in Anabaena sp. PCC 7120 and its effects on cyclic electron flow around photosystem I and tolerances to environmental stresses

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Journal of Integrative Plant Biology 2015年第5期57卷 468-476页

作者： Xiao-Hui jia Peng-Peng Zhang Ding-Ji Shi Hua-Ling Mi jia-cheng Zhu Xi-Wen Huang Pei-Min He Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources Shanghai Ocean UniversityMinistry of Education Shanghai Department of Biological Sciences Louisiana State University Institute of Botany the Chinese Academy of Sciences National Key Laboratory of Plant Molecular Genetics Institute of Plant Physiology and EcologyShanghai Institutes for Biological Sciencesthe Chinese Academy of Sciences

Since pepc gene encoding phosphoenolpyruvate carboxylase （PEPCase） has been cloned from Anabaena sp. PCC 7120 and other cyanobacteria, the effects of pepc gene expression on photosynthesis have not been reported yet. In this study, we constructed mutants containing either upregu-lated （forward） or downregulated （reverse） pepc gene in Anabaena sp. PCC 7120. Results from real‐time quantitative polymerase chain reaction （RT‐qPCR）, Western blot and enzymatic analysis showed that PEPCase activity was signifi-cantly reduced in the reverse mutant compared with the wild type, and that of the forward mutant was obviously increased. Interestingly, the net photosynthesis in both the reverse mutant and the forward mutant were higher than that of the wild type, but dark respiration was decreased only in the reverse mutant. The absorbance changes of P700 upon saturation pulse showed the photosystem I （PSI） activity was inhibited, as reflected by Y（I）, and Y（NA） was elevated, and＆amp;nbsp;dark reduction of P700t was stimulated, indicating enhanced cyclic electron flow （CEF） around PSI in the reverse mutant. Additional y, the reverse mutant photosynthesis was higher than that of the wild type in low temperature, low and high pH, and high salinity, and this implies increased tolerance in the reverse mutant through downregulated pepc gene.

关键词： Anabaena sp. PCC 7120 cyclic electron flow pepc gene photosynthesis tolerance

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Study on the preparation of Pt nanocapsules

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International Journal of Minerals,Metallurgy and Materials 2017年第1期24卷 109-114页

作者： Yi-fan Zhang Zhen Ji Ke Chen Bo-wen Liu cheng-chang jia Shan-wu Yang School of Materials Science and Technology University of Science and Technology BeijingBeijing 100083China

Ag@Pt core-shell nanoparticles（Ag@Pt NPs） were prepared by a co-reduction method. Pt nanocapsules with diameters of less than 10 nm were obtained by an electrochemical method. Cyclic voltammetry（CV） scanning was used to cavitate the Ag@Pt NPs, and the morphology, structure, and cavitation conditions were studied. The results indicate that the effective cavitation conditions to obtain Pt nanoparticles from Ag@Pt NPs are a scanning voltage of 0 to 0.8 V and continuous CV scanning over 2 h. This cavitation method is also applicable for the syntheses of Ir, Ru, and Ru-Pt nanocapsules.

关键词： platinum nanocapsules cavitation preparation methods

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Influence of plasma on the densification mechanism of SPS under multi-field effect

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International Journal of Minerals,Metallurgy and Materials 2014年第9期21卷 906-912页

作者： Yu-hong Chang Di Huang cheng-chang jia Zhao-wen Cui Cong-cong Wang Dong Liang School of Materials Science and Engineering University of Science and Technology Beijing

The densification mechanism of an Fe-based alloy powder containing tiny oxide particles under the synergic multi-field effect of spark plasma sintering （SPS） was investigated. Metallographic microscopy and scanning electron microscopy were used to observe the morphology of samples sintered at different temperatures, and the temperature dislribution in an individual spherical powder particle during sintering was calculated in consideration of the influence of plasma, which was qualified and quantified through the analysis of the U-Icurve. The plasma was observed to play a substantial role in activating and heating the samples at the very early stage of sintering, whereas the joule-heat effect played a dominant role during sintering. Moreover, the plasma also facilitated the diffusion and migration of materials for neck formation.

关键词： iron alloys spark plasma sintering plasma temperature distribution densification diffusion

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Successful treatment of invasive fungal rhinosinusitis caused by Cunninghamella: A case report and review of the literature

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World Journal of Clinical Cases 2019年第2期7卷 228-235页

作者： Yong-Cai Liu Min-Li Zhou Ke-jia cheng Shui-Hong Zhou Xue Wen cheng-Dong Chang Department of Otolaryngology the First Affiliated Hospital College of Medicine Zhejiang University Department of Pathology the First Affiliated Hospital College of Medicine Zhejiang University

BACKGROUND Invasive fungal rhinosinusitis(IFR) caused by Cunninghamella is very rare but has an extremely high fatality rate. There have been only seven cases of IFR caused by Cunninghamella reported in English and, of these, only three patients *** this article, we present another case of IFR caused by Cunninghamella, in which the patient was initially treated successfully but then deteriorated due to a relapse of leukemia 2 mo *** SUMMARY A 50-year-old woman presented with a 2-mo history of right ocular proptosis,blurred vision, rhinorrhea and nasal obstruction. Nasal endoscopic examination showed that the middle turbinate had become necrotic and fragile. Endoscopic sinus surgery and enucleation of the right orbital contents were performed successively. Additionally, the patient was treated with amphotericin B both systematically and topically. Secretion cultivation of the right eye canthus showed infection with Cunninghamella, while postoperative pathology also revealed fungal infection. The patient's condition gradually stabilized after surgery. However, the patient underwent chemotherapy again due to a relapse of leukemia 2 mo later. Unfortunately, her leukocyte count decreased dramatically,leading to a fatal lung infection and *** Aggressive surgical debridements, followed by antifungal drug treatment both systematically and topically, are the most important fundamental treatments for IFR.

关键词： Cunninghamella Invasive fungal rhinosinusitis Acute myeloid leukemia Treatment Prognosis Case report

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Dielectric properties of spark plasma sintered AlN/SiC composite ceramics

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International Journal of Minerals,Metallurgy and Materials 2014年第6期21卷 589-594页

作者： Peng Gao cheng-chang jia Wen-bin Cao Cong-cong Wang Dong Liang Guo-liang Xu School of Materials Science and Engineering University of Science and Technology Beijing

In this study, we have investigated how the dielectric loss tangent and permittivity of AlN ceramics are affected by factors such as powder mixing methods, milling time, sintering temperature, and the addition of a second conductive phase. All ceramic samples were pre-pared by spark plasma sintering （SPS） under a pressure of 30 MPa. AlN composite ceramics sintered with 30wt%-40wt%SiC at 1600℃ for 5 min exhibited the best dielectric loss tangent, which is greater than 0.3. In addition to AlN and β-SiC, the samples also contained 2H-SiC and Fe5Si3, as detected by X-ray difraction （XRD）. The relative densities of the sintered ceramics were higher than 93%. Experimental results indicate that nano-SiC has a strong capability of absorbing electromagnetic waves. The dielectric constant and dielectric loss of AlN-SiC ce-ramics with the same content of SiC decreased as the frequency of electromagnetic waves increased from 1 kHz to 1 MHz.

关键词： ceramic materials composite materials aluminum nitride silicon carbide spark plasma sintering dielectric losses

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Sustainable and practical semi-heterogeneous photosynthesis of 5-amino-1,2,4-thiadiazoles over WS_(2)/TEMPO

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Chinese Chemical Letters 2024年第8期35卷 250-253页

作者： jia-cheng Hou Hong-Tao Ji Yu-Han Lu jia-Sheng Wang Yao-Dan Xu Yan-Yan Zeng Wei-Min He School of Chemistry and Chemical Engineering University of South ChinaHengyang 421001China

An eco-friendly and practical method for the clean preparation of 5-amino-1,2,4-thiadiazoles was *** WS_(2)as the semiconductor photocatalyst,both TEMPO and O_(2)(in air)as the redox catalysts,a variety of thiadiazoles were semi-heterogeneously formed in high to quantitative yields and could be easily collected by CPME extraction and ***,the catalytic system can be reusable for at least 5 reaction runs.

关键词： Green chemistry Thiadiazoles Heterogeneous catalyst Redox catalyst Extraction

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Detection of ryanodine receptor target-site mutations in diamide insecticide-resistant Spodoptera frugiperda in China

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Insect Science 2021年第3期28卷 639-648页

作者： Sheng-Lan Lv Yang Shi jia-cheng Zhang Pei Liang Lei Zhang Xi-Wu Gao College of Plant Protection China Agricultural UniversityBeijingChina

Spodoptera frugiperda(Lepidoptera:Noctuidae)is a widely distributed pest of *** it invaded China in 2018,it has caused serious damage to local corn ***,an anthranilic diamide insecticide,has been widely used to control lepidopteran *** is a new allosteric modulator insecticide devel-oped based on chlorantraniliprole,so it has a similar mechanism and insecticidal *** this study,we investigated resistance levels to chlorantraniliprole and tetrachloropyramid in *** from 13 populations in *** the populations tested,the relative highest resistance to chlorantraniliprole occurred in the Guangzhou population,and the most susceptible to chlorantraniliprole was found in the Wuhan *** lethal dosage LD50 value of the Guangzhou population against chlorantraniliprole was 27.8-fold higher than that of the Wuhan *** differences were observed among 5".frugiperda populations in terms of sensitivity to *** mutations at the 14734 site of the ryanodine receptor(RyR)were found,while no mutations were found in the G4891 *** mutations were detected in only two of the 786 individuals analyzed one from the Qinzhou population and other from the Anshun population(frequency below 2%in both cases).There were no significant differences in the expression levels of RyR between Guangzhou and Wuhan *** summary,our results indicate that:(i)*** has low resistance levels to diamide insecticides in China;and(ii)the differences in relative resistance among the 13 populations analyzed are not caused by the mutations in RyR or the expression of RyR.

关键词： diamide insecticides resistance resistance risk Spodoptera frugiperda

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Allergic fungal rhinosinusitis accompanied by allergic bronchopulmonary aspergillosis: A case report and literature review

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World Journal of Clinical Cases 2019年第22期7卷 3821-3831页

作者： Ke-jia cheng Min-Li Zhou Yong-Cai Liu Shui-Hong Zhou Department of Otolaryngology The First Affiliated Hospital College of Medicine Zhejiang University

BACKGROUND Concomitant allergic fungal rhinosinusitis(AFRS)and allergic bronchopulmonary aspergillosis(ABPA)are extremely rare,with no more than 20 cases reported in the English *** SUMMARY A 52-year-old female patient complained of right-sided nasal obstruction,rhinorrhea,sneezing,epistaxis,and hyposmia for a period of around 5 *** examination detected paleness and edema of the nasal mucous membrane and a polyp in the right middle meatus.A computed tomography(CT)scan of the sinuses revealed a ground-glass opacity filling the right maxillary and ethmoid sinuses,along with bone absorption in the medial wall of the right maxillary *** resonance images were obtained with T1-weighted,T2-weighted,and gadolinium-enhanced T1-weighted sequences.A well-defined mass,located in the right maxillary and ethmoid sinuses and displaying obvious hypointense features,was observed on both T1-and T2-weighted images,with peripheral enhancement on gadolinium-enhanced T1-weighted *** patient also has a 20-year history of cough and *** CT revealed columned and cystiform bronchiectasis in the bilateral bronchus,surrounded by a large number of spotted and funicular high-density *** level of serum total IgE was>5000 kU/*** IgE levels related to house dust and aspergillus showed a positive result,with the values being 3.5 kU/L and 1.2 kU/*** performed functional endoscopic sinus surgery under local *** surgery,topical glucocorticoids and saline irrigation were applied in the nasal cavity until the present *** oral glucocorticoid(methylprednisolone 16 mg/d)and antifungal agent(itraconazole 200 mg/d)were also used for a period of 4 *** was prescribed at 10 mg/d until the present *** endoscopic examination showed that the patient was recovering well at 3 mo after *** Since different specialists treat ABPA and AFRS,their coexistence may be *** accompanied by ABPA requires surgical t

关键词： Allergic fungal rhinosinusitis Allergic bronchopulmonary aspergillosis Aspergillus Clinical characteristics Treatment Surgery

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Fabrication of tungsten films by metallorganic chemical vapor deposition

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International Journal of Minerals,Metallurgy and Materials 2012年第12期19卷 1149-1153页

作者： Yi Li Jin-pu Li cheng-chang jia Xue-quan Liu School of Materials Science and Engineering University of Science and Technology Beijing Beijing 100083 China Powder Metallurgy Laboratory Central Iron & Steel Research Institute Beijing 100081 China

Tungsten films growing on copper substrates were fabricated by metallorganic chemical vapor deposition （MOCVD）. The chemi-cal purity, crystallographic phase, cross-sectional texture, and resistivity of the deposited films both before and after annealing treatment were investigated by X-ray energy-dispersive spectroscopy （EDS）, X-ray diffraction （XRD）, scanning electron microscopy （SEM）, and four-point probe method. It is found that the films deposited at 460°C are metastable β-W with （211） orientation and can change into α-W when an-nealed in high-purity hydrogen atmosphere at high temperature. There are small amounts of C and O in the films, and the W content of the films increases with increasing deposition temperature and also goes up after annealing in high-purity hydrogen atmosphere. The films have columnar microstructures and the texture evolution during their growth on copper substrates can be divided into three stages. The resistivity of the as-deposited films is in the range of 87-104 μΩ·cm, and low resistivity is obtained after annealing in high-purity hydrogen atmosphere.

关键词： thin films tungsten metallorganic chemical vapor deposition crystallography textures electric properties

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Accumulation of cadmium and its effects on growth, development and hemolymph biochemical compositions in Boettcherisca peregrina larvae （Diptera： Sarcophagidae）

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Insect Science 2006年第1期13卷 31-39页

作者： GUO-XING WU GONG-YIN YE CUI HU jia-AN cheng Institute of Applied Entomology College of Agriculture and Biotechnology Zhejiang University Hangzhou China

Newly emerged larvae of the fleshfly, Boettcherisca peregrina were exposed to two different CdCl2 concentrations of 100μg/g and 400 mg/g diet fresh weight （DFW）. They were administered in the diets until the end of larval stage. Cd-exposed larvae accumulated significant amounts of Cd and this accumulation increased with the exposure dose and time. The body weights were lightened and lengths of larvae were shortened considerably after Cd exposure, especially at the higher Cd concentration. The total larval duration was also extremely affected due to Cd exposure. The average duration was prolonged significantly by 14 h at the lower Cd concentration, while it was increased by 33.7 h over controls at the higher Cd concentration. A significant decrease in contents of either soluble proteins, total lipids or caloric values in the hemolymph occurred due to Cd exposure throughout the entire tested period but after 120 h of Cd exposure. In contrast, when exposed to Cd with its higher concentration, total sugar contents in the hemolymph were increased strikingly over the whole tested time, except after 96 h of Cd exposure, while they were not apparently altered except after 24 h of Cd exposure at the lower concentration. Thus, it is suggested that Cd exposure shows significant adverse impact on the growth and development, as well as metabolism, in larvae of this fleshfly, depending on its exposure time and dose.

关键词： Boettcherisca peregrina cadmium caloric value growth and development,lipids proteins total sugar

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