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基于CT多平面重建的原发性肠脂垂炎3例报道

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云南医药 2024年第3期45卷 119-120页

作者：郭凡明邓承彭佩乾怒江傈僳族自治州人民医院放射科云南怒江673100 昆明医科大学第二附属医院放射科云南昆明650032

1临床资料病例1:男,40岁,无明显诱因出现左下腹疼痛4天,为间断性刺痛,疼痛与进食无关,无畏寒发热,以“腹痛原因待查”入院,患者血常规检查示:白细胞、淋巴细胞计数、中性粒细胞等均为阴性,行CT平扫(多平面重建)示:左下腹结肠降段与乙状... 详细信息

1临床资料病例1:男,40岁,无明显诱因出现左下腹疼痛4天,为间断性刺痛,疼痛与进食无关,无畏寒发热,以“腹痛原因待查”入院,患者血常规检查示:白细胞、淋巴细胞计数、中性粒细胞等均为阴性,行CT平扫(多平面重建)示:左下腹结肠降段与乙状结肠交界区腹侧下方可见一椭圆形脂肪密度影,边缘呈环形等密度、与周围脂肪分界清楚,多平面重建示病灶大小约1.5×1.2×1.0cm,见图1A (冠状位);病灶内见细线状等密度影,见图1B (横断位);病灶内见小点状等密度影;图1C示CT增强示病灶边缘轻度强化,其内小点状或条状等密度影未见明显强化。诊断为:左下腹降结肠及乙状结肠交界区脂肪密度影,多考虑结肠脂垂炎。给予对症治疗后好转出院。

关键词： CT 多平面重建原发性肠脂垂炎

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基于人工智能的运营商故障分析能力提升研究

邮电设计技术 2024年第6期 72-77页

作者：朱宏邓程王瑜宋文杰中国联通智网创新中心江苏南京210019

传统的故障分析手段将运维经验固化为故障分析规则或脚本,这种方式针对特定故障模式较为有效,但是无法应对新网络业务、组网变化,一旦规则、脚本需要调整,需投入较大成本进行适配改造,且时效性较差。而人工智能技术在大数据统计、分析... 详细信息

传统的故障分析手段将运维经验固化为故障分析规则或脚本,这种方式针对特定故障模式较为有效,但是无法应对新网络业务、组网变化,一旦规则、脚本需要调整,需投入较大成本进行适配改造,且时效性较差。而人工智能技术在大数据统计、分析、推理、自适应学习上有着先天优势,能快速基于新网络、新业务的变化重训练AI模型参数,给出最佳推荐值。基于此,重点研究了如何利用人工智能提升运营商故障分析能力。

关键词：故障分析智能分析人工智能大数据网络运维

Drain-site hernia after laparoscopic rectal resection:A case report and review of literature

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World Journal of Clinical Cases 2022年第8期10卷 2637-2643页

作者： Jin Su cheng deng Hui-Ming Yin Department of General Surgery Zhuzhou Central HospitalThe Affiliated Zhuzhou Hospital of Xiangya Medical College of Central South UniversityZhuzhou 412000Hunan ProvinceChina Division of Science and Education Zhuzhou Central HospitalThe Affiliated Zhuzhou Hospital of Xiangya Medical College of Central South UniversityZhuzhou 412000Hunan ProvinceChina Department of General Surgery the Second Affiliated Hospital of Hunan University of Traditional Chinese MedicineChangsha 410000Hunan ProvinceChina

BACKGROUND Drain-site hernia(DSH)has an extremely low morbidity and has rarely been *** bowel obstruction is a frequent concurrent condition in most cases of DSH,which commonly occurs at the≥10 mm *** we report a rare case of DSH at the lateral 5 mm port site one month postoperatively without visceral ***,a brief review of the literature was conducted focusing on the risk factors,diagnosis,and prevention strategies for *** SUMMARY A 76-year-old male patient was admitted to our institution with intermittent abdominal pain and a local abdominal mass which occurred one month after laparoscopic radical resection of rectal cancer one year ago.A computed tomography scan showed an abdominal wall hernia at the 5 mm former drain-site in the left lower quadrant,and that the content consisted of the large *** elective herniorrhaphy was performed by closing the fascial defect and reinforcing the abdominal wall with a synthetic mesh *** postoperative period was *** patient was discharged seven days after the operation without surgery-related complications at the 1-mo follow-up *** Emphasis should be placed on DSH despite the decreased use of intra-abdominal *** is recommended that placement of a surgical drainage tube at the≥10 mm trocar site should be ***,it is advisable to have a comprehensive understanding of the risk factors for DSH and complete closure of the fascial defect at the drainage site for high-risk patients.

关键词： Drain-site hernia Abdominal hernia Laparoscopic surgery Surgical drainage Risk factor Case report

Animal secretory endolysosome channel discovery

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Zoological Research 2021年第2期42卷 141-152页

作者： Yun Zhang Qi-Quan Wang Zhong Zhao cheng-Jie deng Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences&Yunnan Province Kunming Institute of ZoologyChinese Academy of SciencesKunmingYunnan 650223China Center for Excellence in Animal Evolution and Genetics Chinese Academy of SciencesKunmingYunnan 650223China

Secretory pore-forming proteins(PFPs) have been identified in organisms from all kingdoms of life. Our studies with the toad species Bombina maxima found an interaction network among aerolysin family PFPs(af-PFPs) and trefoil factors(TFFs). As a toad af-PFP, Bm ALP1 can be reversibly regulated between active and inactive forms, with its paralog Bm ALP3 acting as a negative regulator. Bm ALP1 interacts with Bm TFF3 to form a cellular active complex called βγ-CAT. This PFP complex is characterized by acting on endocytic pathways and forming pores on endolysosomes, including stimulating cell macropinocytosis. In addition, cell exocytosis can be induced and/or modulated in the presence of βγ-CAT. Depending on cell contexts and surroundings, these effects can facilitate the toad in material uptake and vesicular transport, while maintaining mucosal barrier function as well as immune defense. Based on experimental evidence,we hereby propose a secretory endolysosome channel(SELC) pathway conducted by a secreted PFP in cell endocytic and exocytic systems, with βγ-CAT being the first example of a SELC protein. With essential roles in cell interactions and environmental adaptations, the proposed SELC protein pathway should be conserved in other living organisms.

关键词： Pore-forming protein Secretory endolysosome channel(SELC) Endocytosis Exocytosis Vesicular transport

LABEL-FREE DETECTION OF PROTEIN MICROARRAY WITH HIGH THROUGHPUT SURFACE PLASMON RESONANCE IMAGING(SPRI)

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Journal of Innovative Optical Health Sciences 2008年第1期1卷 107-114页

作者： SHUKUAN XU GUOLIANG HUANG cheng deng JIANG ZHU CHAO HAN XIAOYONG YANG Medical Systems Biology Research Center Tsinghua University School of Medicine Beijing China National Engineering Research Center for Beijing Biochip Technology BeijingChina

A surface plasmon resonance imaging(SPRI)system was developed for the discrimination of proteins on a gold *** a label-free and high-throughput technique,SPRI enables simultaneously monitoring of the biomolecular interactions at low *** used SPRI as a label-free and parallel method to detect different proteins based on protein *** Serum Albumin(BSA),Casein and Immunoglobulin G(IgG)were immobilized onto the Au surface of a gold-coated glass chip as spots forming a 6×6 *** proteins can be discriminated directly by changing the incident angle of *** reproducibility for label-free detection of protein molecules was *** SPRI platform represents a simple and robust method for performing high-sensitivity detection of protein microarray.

关键词： Surface plasmon resonance imaging protein microarray label-free highthroughput

Construction and Characterization of a cDNA Library from the Pulp of Cara Cara Navel Orange （Citrus sinensis Osbeck）

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Journal of Integrative Plant Biology 2006年第3期48卷 315-319页

作者： Neng-Guo Tao Juan Xu Yun-Jiang cheng Xiu-Xin deng State Key Laboratory of Crop Genetic Improvement National Center of Citrus Breeding Huazhong Agricultural University Wuhan 430070 China

A cDNA library was constructed end characterized from the pulp of Cera Care navel orange （Citrus sinensis Osbeck） at different stages of ripening. Tittering results revealed that approximately 5.086×10^5 independent clones were included in this library. Electrophoresls gel results of 15 randomly selected clones revealed that the size of the insertion fragments ranged from 400 bp to 2 kb, with an average size of 900 bp. Sequencing results of 150 randomly picked clones showed that the recombination rate was 94%. During subsequent sequence analysis, 41 of 139 clones failed to be identified end the amino sequence of 71 clones shared less than 30% identity with related plants in GenBank. Of 27 clones whose amino sequences shared more than 60% identity with other related plants in GenBenk, 17 clones showed an 80% identity with the corresponding candidate genes of citrus. The clone recognized as the type Ⅲ metallothlonein-llke （MT） gene was observed to occur 13 tlmes, Indlcatlng that the protein may play an important role In frult development and rlpenlng.

关键词： Cara Cara navel orange cDNA library Citrus, pulp.

DETECTION AND APPLICATION OF MICROFLUIDIC ISOTHERMAL AMPLIFICATION ON CHIP

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Journal of Innovative Optical Health Sciences 2008年第2期1卷 257-265页

作者： GUOLIANG HUANG XIAOYONG YANG JIANG ZHU SHUKUAN XU cheng deng CHAO HAN Medical Systems Biology Research Center Tsinghua University School of Medicine Beijing 100084China National Engineering Research Center for Beijing Biochip Technology Beijing 102206China

Loop-mediated isothermal amplification(LAMP)is a novel nucleic acid amplification *** with the widely utilized polymerase chain reaction(PCR),LAMP has higher speed and efficiency as well as lower requirement for system temperature control because the whole amplification process is isothermal and no efforts are needed to switch between different *** this paper,we designed and fabricated different kinds of polycarbonate(PC)microfluid chips,explored appropriate reaction condition for LAMP in microenvironment(1 nL→10μL),and developed a microfluidic isothermal amplification detection *** DNA optimal amplification temperature is obtained;the starting time of exponential amplification of DNA is put forward *** optimal condition of DNA amplification in microenvironment,with a little reaction materials and early starting exponential amplification time of DNA are very important for clinic DNA detection and the application of Lab-on-a-Chip.

关键词： Loop-mediated isothermal amplification Lab-on-a-Chip microfluid chips polymerase chain reaction DNA amplification

Metabolomic characteristics of spontaneously hypertensive rats under chronic stress and the treatment effect of Danzhi Xiaoyao Powder, a traditional Chinese medicine formula

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Journal of Integrative Medicine 2022年第1期20卷 73-82页

作者： Shuai Chen Jin Hu deng-cheng Lu Hong-yi Liu Shan-shan Wei College of Basic Medical Sciences Yunnan University of Chinese MedicineKunming 650500Yunnan ProvinceChina Department of Endocrinology Yunnan Provincial Hospital of Traditional Chinese MedicineKunming 650021Yunnan ProvinceChina Key Laboratory of Syndrome Micro-Differentiation of Yunnan Province Kunming 650500Yunnan ProvinceChina

Objective: Numerous studies have demonstrated the close relationship between chronic stress and blood pressure(BP). Hypertensive subjects exhibit exaggerated reactions to stress, especially higher BP. The mechanisms by which stress affects pre-existing hypertension still need to be explored. Danzhi Xiaoyao Powder(DP), a historical traditional Chinese medicine formula, is a promising treatment for BP control in hypertensive patients under stress. The present study investigated the metabolomic disruption caused by chronic stress and the treatment effect and mechanism of ***: Spontaneously hypertensive rats(SHRs) were subjected to chronic restraint stress(CRS) for 4 weeks. BP was measured via the tail-cuff method, and anxiety-like behavior was quantified using the elevated-plus-maze test. Meanwhile, DP was administered intragastrically, and its effects were observed. Global metabolomic analysis was performed using ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry, followed by multivariate statistical analysis to detect differential metabolites and ***: DP alleviated the CRS-induced increase in BP and anxiety-like behavior. Systematic metabolic differences were found among the three study groups. A total of 29 differential plasma metabolites were identified in both positive-and negative-ion modes. These metabolites were involved in triglyceride metabolism, amino acid(phenylalanine, tryptophan, and glycine) metabolism, and steroid hormone ***: These findings expose the metabolomic disturbances induced by chronic stress in SHRs and suggest an innovative treatment for this disorder.

关键词： Hypertension Stress Metabolomics Danzhi Xiaoyao Powder

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过氧化氢催化分解材料及反应机理的研究进展

应用化工 2024年第7期53卷 1652-1657页

作者：李海云商怡然邓橙衣颖吴金辉赵蕾朱孟府军事科学院系统工程研究院卫勤保障技术研究所天津300161

过氧化氢消毒因具有强氧化性、低腐蚀性、优秀的渗透性和扩散性等优势,已成为最具前景的空间消毒技术之一。然而消毒结束后,环境中残留的高浓度过氧化氢不仅易致爆,还会对人体产生不可逆的危害,因此开展过氧化氢催化分解技术的研究具有... 详细信息

过氧化氢消毒因具有强氧化性、低腐蚀性、优秀的渗透性和扩散性等优势,已成为最具前景的空间消毒技术之一。然而消毒结束后,环境中残留的高浓度过氧化氢不仅易致爆,还会对人体产生不可逆的危害,因此开展过氧化氢催化分解技术的研究具有重要意义。总结了目前常用的过氧化氢催化分解材料及其研究应用现状,概述了过氧化氢在催化剂表面的分解反应机理及其研究动态,并指出了目前过氧化氢催化分解技术存在的主要问题和研究重点,为高性能过氧化氢催化剂的设计和开发提供理论支撑,最后对催化剂的未来发展方向进行了展望。

关键词：过氧化氢(H_(2)O_(2)) 催化分解催化剂反应机理