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影像科学与光化学 2010年第2期28卷 131-136页

作者：谭广慧魏树权赵俊明张鹏王丙南郑鹏刘岩金英学哈尔滨师范大学化学化工学院黑龙江哈尔滨150025

邻苯二甲酰化的二肽(1)在光照下,经过单电子转移形成分子内双自由基(5),自由基(5)分子内耦合成环得到含哌嗪酮的化合物.此反应产率较好,可以成为合成哌嗪酮类化合物的新方法.所有新化合物均经NMR和MS确定其结构.

关键词：二肽单电子转移环化反应哌嗪酮

SRAP分析体系的优化及在枇杷种质资源研究上的应用

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果树学报 2008年第3期25卷 348-352页

作者：乔燕春林顺权刘成明杨向晖华南农业大学园艺学院广州510642

以me7(5’-TGAGTCCAAACCGGTCC-3’)和em7(5’-GACTGCGTACGAATTCAA-3’)正反向引物组合对西班牙枇杷品种Javierin进行了SRAP分析体系的优化,结果表明,在25μL反应体系中,5种主要成分的适宜浓度或用量分别是:dNTPs0.3mmol/L,Mg2+2.5mmol/L,TaqDNA聚合酶1.0U,引物0.3μmol/L,模板DNA20ng,优化的扩增程序为:94℃预变性5min,94℃变性1min,35℃复性1min,72℃延伸1min30s,5个循环;之后94℃变性1min,50℃复性1min,72℃延伸1min30s,35个循环;最后72℃下延伸10min。并将该优化的体系在来自中国、西班牙、日本、意大利和美国的46份枇杷种质资源上进行了SRAP扩增的初步应用,经琼脂糖和聚丙烯酰胺凝胶电泳均获得了清晰、重复性好的SRAP指纹图谱。

关键词：枇杷种质资源 SRAP 体系优化

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经颅电刺激运动诱发电位监测麻醉方法的建立

中华神经外科杂志 2010年第12期26卷 1090-1093页

作者：刘海洋程灏金海龙王明然刘淑玲乔慧韩如泉首都医科大学附属北京天坛医院麻醉科 100050 北京市神经外科研究所电生理室

目的对比相同麻醉深度下丙泊酚、七氟醚分别复合瑞芬太尼维持麻醉对经颅电刺激运动诱发电位（TES-MEPs）波幅和潜伏期的影响,以建立术中MEP监测时的适宜麻醉方法.方法 36例脊髓脊柱神经外科手术患者随机分为两组：丙泊酚麻醉组和七氟... 详细信息

目的对比相同麻醉深度下丙泊酚、七氟醚分别复合瑞芬太尼维持麻醉对经颅电刺激运动诱发电位（TES-MEPs）波幅和潜伏期的影响,以建立术中MEP监测时的适宜麻醉方法.方法 36例脊髓脊柱神经外科手术患者随机分为两组：丙泊酚麻醉组和七氟醚麻醉组,每组18例.对比麻醉诱导后30 min、60 min、90 min和120 min MEP的波幅和潜伏期.结果两组病例麻醉期间血流动力学均维持稳定,组间脑电双频指数、呼气末二氧化碳分压和体温的差异均无统计学意义.丙泊酚组MEP所需阈刺激强度显著低于七氟醚组[（172±23）V：（217±42）V,P＜0.05].丙泊酚组各时间点上肢MEP波幅显著高于七氟醚组（P＜0.05）,潜伏期显著短于七氟醚组（P＜0.05）.两组内各时间点上肢MEP波幅和潜伏期差异无统计学意义.结论在相同麻醉深度下,丙泊酚复合瑞芬太尼全凭静脉麻醉对MEP波幅的抑制作用显著优于七氟醚复合瑞芬太尼静吸复合麻醉方案,是用于脊髓脊柱手术TES-MEPs监测的适宜麻醉方法.

关键词：运动诱发电位神经外科麻醉七氟醚丙泊酚

祖厉河流域1:50000与1:250000比例尺DEM信息容量的比较

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干旱区研究 2007年第1期24卷 131-134页

作者：刘慧明许宝泉李凤民阎天成孙国钧兰州大学干旱与草地生态教育部重点实验室甘肃兰州730000 甘肃省水利厅农村水利处甘肃兰州730000

以甘肃省祖厉河流域为实验样区,以1∶50 000 DEM为基准,运用比较分析与数理统计的方法,研究该区域1∶250 000 DEM描述地表形态的精度特征。基本技术平台为ArcGIS 8.3软件。研究结果表明,利用1∶250 000DEM所提取的流域各级河网、地面坡... 详细信息

以甘肃省祖厉河流域为实验样区,以1∶50 000 DEM为基准,运用比较分析与数理统计的方法,研究该区域1∶250 000 DEM描述地表形态的精度特征。基本技术平台为ArcGIS 8.3软件。研究结果表明,利用1∶250 000DEM所提取的流域各级河网、地面坡度、地面坡向3种因子与基于1∶50 000 DEM所提取的各因子相比较,均存在不同程度的信息损失,1∶50 000 DEM能较好地模拟真实的地面信息。研究成果为半干旱区集水农业工程的规划和水土保持等应用标准的制定提供了理论依据。

关键词：数字高程模型比例尺地形因子精度甘肃

A practical community-based response strategy to interrupt Ebola transmission in sierra Leone, 2014–2015

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Infectious Diseases of Poverty 2016年第1期5卷 670-679页

作者： Zhong-Jie Li Wen-Xiao Tu Xiao-Chun wang Guo-Qing Shi Zun-Dong Yin Hai-Jun Su Tao Shen Da-Peng Zhang Jian-Dong Li Shan Lv Chun-Li Cao Rui-Qian Xie Hong-Zhou Lu Rong-Meng Jiang Zheng Cao Zhi-Jie An Lei-Lei Li Jie Xu yan-Wen Xiong Wei Zang Wei Zhang Hong-Wei Zhang Wen-Sen Chen Hua Ling Wen Xu Jian Cai Huan-Jin Luo Xue-Sheng Xing Can-Jun Zheng Qiang Wei Xin-Xu Li Mei Li Hai Jiang Li-quan Deng ming-quan Chen Xiang Huo Feng Xu Xue-hui Lai Xi-Chen Bai Long-Jie Ye Jian-Yi Yao Wen-Wu Yin Jiao-Jin Sun Lin Xiao Fu-Qiang liu Xiao-Qiang liu Hong-Wei Fan Zeng-Qiang Kou Ji-Kun Zhou Hao Zhang Da-Xin Ni Thomas TSamba Qun Li Hong-Jie Yu Yu wang Xiao-Feng Liang Division of Infectious Disease Key Laboratory of Surveillance and Early-warning on Infectious DiseaseChinese Center for Disease Control and PreventionBeijingChina Public Health Emergency Center Chinese Center for Disease Control and PreventionBeijingChina National Center for AIDS/STD Control and Prevention Chinese Center for Disease Control and PreventionBeijingChina Chinese Field Epidemiology Training Program Chinese Center for Disease Control and PreventionBeijingChina National Immunization Program Chinese Center for Disease Control and PreventionBeijingChina Bureau of Disease Prevention and Control National Health and Family Planning Commission of the People’s Republic of ChinaBeijingChina National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and PreventionBeijingChina National Institute of Parasitic Disease Chinese Center for Disease Control and PreventionShanghaiChina Chinese Center for Health Education BeijingChina Department of Infectious Diseases Shanghai Public Health Clinical CenterShanghaiChina Beijing Ditan Hospital Capital Medical UniversityBeijingChina Health News BeijingChina National Institute for Communicable Disease Control and Prevention Chinese Center for Disease Control and PreventionBeijingChina Department of Training Chinese Center for Health EducationBeijingChina Center for Infectious Diseases Beijing Youan HospitalCapital Medical UniversityBeijingChina Infection Management The First Affiliated Hospital of Nanjing Medical UniversityNanjingChina Chongqing Municipal Center for Disease Control and Prevention ChongqingChina Yunnan Provincial Center for Disease Control and Prevention KunmingChina Division of Infectious Disease Control and Prevention Zhejiang Provincial Center for Disease Control and PreventionHangzhouChina Guangdong Provincial Center for Disease Control and Prevention GuangzhouChina Division of Acute Infectious Disease Control and Prevention Hubei Provincial Center for Disease Control and PreventionW

Background:The Ebola virus disease spread rapidly in West Africa in 2014,leading to the loss of thousands of *** engagement was one of the key strategies to interrupt Ebola transmission,and practical community level measures needed to be explored in the field and tailored to the specific context of ***:First,community-level education on Ebola virus disease(EVD)prevention was launched for the community’s social mobilizers in six districts in Sierra Leone beginning in November ***,from January to May of 2015,in three pilot communities,local trained community members were organized to engage in implementation of EVD prevention and transmission interruption measures,by involving them in alert case report,contact tracing,and social *** epidemiological indicators of transmission interruption in three study communities were ***:A total of 6016 community social mobilizers from 185 wards were trained by holding 279 workshops in the six districts,and EVD message reached an estimated 631680 *** three pilot communities,72 EVD alert cases were reported,with 70.8%of them detected by trained local community members,and 14 EVD cases were finally *** tracing detected 64.3%of EVD *** median duration of community infectivity for the cases was 1 *** secondary attack rate was 4.2%,and no third generation of infection was *** health worker was infected,and no unsafe burial and noncompliance to EVD control measures were *** community-based measures were modeled to reduce 77 EVD cases,and the EVD-free goal was achieved four months earlier in study communities than whole country of Sierra ***: The community-based strategy of social mobilization and community engagement was effective in casedetection and reducing the extent of Ebola transmission in a country with weak health system. The successfullypractical experience to reduce the risk of Ebola transmission in the

关键词： Ebola virus disease Community engagement Health education Outbreak control

CB-GFP融合基因在COS-7细胞中的表达与定位

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军事医学科学院院刊 2005年第6期29卷 516-519,522页

作者：甘慧周勇孙萍王嘉军刘敏霞高明孙红琰王全立军事医学科学院野战输血研究所北京100850

目的:构建以绿色荧光蛋白(green fluorescent prote in,GFP)为报告基因的重组表达质粒pEGFP-N1-CB,转染体外培养的COS-7细胞,以观察CB重组蛋白在真核细胞中的表达及定位。方法:PCR方法扩增得到去除终止密码的CB融合基因序列,克隆入真核... 详细信息

目的:构建以绿色荧光蛋白(green fluorescent prote in,GFP)为报告基因的重组表达质粒pEGFP-N1-CB,转染体外培养的COS-7细胞,以观察CB重组蛋白在真核细胞中的表达及定位。方法:PCR方法扩增得到去除终止密码的CB融合基因序列,克隆入真核表达载体pEGFP-N1中,构建重组表达载体pEGFP-N1-CB。脂质体法转染体外培养的COS-7细胞后,以RT-PCR和W estern印迹方法验证其mRNA及蛋白的表达,并在活细胞状态下用荧光显微镜、激光共聚焦显微成像技术直接观察CB-GFP融合蛋白在细胞中的分布和定位。结果:RT-PCR及W estern印迹结果均证明CB-GFP融合基因表达载体pEGFP-N1-CB在COS-7细胞中获得了表达。荧光显微镜观察显示,在空载体pEGFP-N1转染组中,COS-7细胞内荧光呈弥散分布;重组质粒pEGFP-N1-CB转染组中,绿色荧光主要聚集在细胞浆中。结论:CB融合基因能在真核细胞COS-7中得到高效表达,且蛋白表达主要定位于细胞浆中,本试验为CB重组蛋白的提取及进一步功能研究奠定了基础。

关键词：绿色荧光蛋白 CB融合基因重组蛋白质类真核细胞基因表达共聚焦转染

金黄色葡萄球菌感染中IL-17的分子机制研究进展

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中国感染控制杂志 2023年第6期22卷 731-737页

作者：崔岩王天明付琦吴志航李日成陈全鑫刘美王波潘海邦甘肃中医药大学第一临床医学院甘肃兰州730000 甘肃中医药大学护理学院甘肃兰州730000

金黄色葡萄球菌(SA)是当前全球常见的感染性致病菌,抗菌药物的滥用导致SA感染的预防和治疗更加困难。寻求新型有效的抗菌药物替代品或者疫苗至关重要,这也是目前乃至未来研究的热点。大量研究报道白细胞介素-17(IL-17)对于SA的清除和预... 详细信息

金黄色葡萄球菌(SA)是当前全球常见的感染性致病菌,抗菌药物的滥用导致SA感染的预防和治疗更加困难。寻求新型有效的抗菌药物替代品或者疫苗至关重要,这也是目前乃至未来研究的热点。大量研究报道白细胞介素-17(IL-17)对于SA的清除和预防至关重要,IL-17家族种类繁多、机制复杂,人体IL-17与SA携带之间的联系虽尚未被广泛研究,但大多数的动物试验结果也带来了克服SA感染的希望。IL-17信号通路的优势也驱使着越来越多的科研人员开始关注其在免疫方面的作用,有望成为抗SA疫苗制剂的关键靶点。本文对IL-17及其信号通路、与SA感染的分子机制的研究进展进行综述。

关键词：金黄色葡萄球菌白细胞介素-17 分子机制感染免疫 IL-17

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T3期声门型喉癌的外科治疗选择

中华肿瘤杂志 2011年第11期33卷 860-863页

作者：温树信王斌全刘涛皇甫辉张海利张春明高伟冯彦山西医科大学第一医院耳鼻咽喉头颈外科太原030001

目的探讨T3期声门型喉癌合适的外科治疗方式。方法回顾性分析57例乃期声门型喉癌患者的临床病理特征、手术方式和预后，分析不同手术方式和不同年龄组患者的3年无瘤生存率。结果57例仍期声门型喉癌患者全部行喉部手术治疗，失访7例，总... 详细信息

目的探讨T3期声门型喉癌合适的外科治疗方式。方法回顾性分析57例乃期声门型喉癌患者的临床病理特征、手术方式和预后，分析不同手术方式和不同年龄组患者的3年无瘤生存率。结果57例仍期声门型喉癌患者全部行喉部手术治疗，失访7例，总的3年无瘤生存率为63．2％（36／57）。采用喉全切除术24例，喉近全切除术8例，喉部分切除术25例，3年无瘤生存率分别为66．7％（16／24）、50．0％（4／8）和64．0％（16／25），差异无统计学意义（P=0．694）。970岁者和〈70岁者的3年无瘤生存率分别为70．0％（7／10）和61．7％（29／47），差异亦无统计学意义（P=0．621）。36例患者行颈淋巴结清扫术，其中全颈淋巴结清扫2例，改良颈淋巴结清扫6例，分区性颈淋巴结清扫28例，颈部淋巴结转移率为17．5％（10／57）。局部复发10例，其中喉部分切除术后7例，喉近全切除术后2例，喉全切除术后1例。结论喉全切除术与不同的喉部分切除术均是仍期声门型喉癌的重要手术方式，应根据患者局部病变与全身状况的具体情况综合考虑，选择合适的个体化手术方式。≥70岁的B期声门型喉癌患者，应慎行喉部分切除术，喉全切除术也可以取得满意疗效。

关键词：喉肿瘤癌,鳞状细胞声门喉切除术预后

新型冠状病毒肺炎疫情期口腔医院防护标准(七)——生物气溶胶的防控

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实用口腔医学杂志 2020年第2期36卷 184-187页

作者：李广文李卉李刚王瑞常蓓侯锐刘雯刘谢诚刘蕊孔亮张铭军事口腔医学国家重点实验室口腔疾病国家临床医学研究中心陕西省口腔疾病国际联合研究中心第四军医大学口腔医院西安710032 西南医科大学公共卫生学院第四军医大学唐都医院第四军医大学火箭军特色医学中心

在口腔诊疗过程中产生的生物气溶胶携带的病原体对口腔医务人员具有潜在感染风险,本文阐述了气溶胶的危害、产生来源、传染疾病特点及预防控制措施等,以期提高新型冠状病毒肺炎流行期口腔医务人员防护意识,加强防护措施减少感染风险。

关键词：新型冠状病毒肺炎生物气溶胶防控

Proportion of Uterine Malignant Tumors in Patients with Laparoscopic Myomectomy： A National Multicenter Study in China

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Chinese Medical Journal 2017年第22期130卷 2661-2665页

作者： Hua yang Xiao-Chuan Li Chen Yao Jing-He Lang Hang-Mei Jin ming-Rong Xi Gang wang Lu-Wen wang Min Hao yan Ding Jie Chen Jian-Qing Zhang Lu Han cheng-Xiu Guo Xiang Xue yan Li Jian-Hua Zheng Man-Hua Cui Huai-Fang Li Guang-Shi Tao Long Chen Su-Min wang An-Wei LU Ze-Hua Huang Qing liu Ya-Li Zhuang Xiang-Hua Huang Gen-Hai Zhu Ou-Ping Huang Li-Na Hu Mu-Jun Li Hong-Lin Zhou Jing-hui Song Lan Zhu Department of Obstetrics and Gynecology Peking Union Medical College Hospital Beijing 100730 China Department of Biostatistics Peking University Clinical Research Institute Peking University First Hospital Beijing 100871 China Department of Obstetrics and Gynecology Zhejiang University Affiliated Obstetrics and Gynecology Hospital Zhejiang Hangzhou 310006 China Department of Obstetrics and Gynecology West China Second Hospital of Sichuan University Cbengdu Sichuan 610041 China Department of Obstetrics and Gynecology The First People's Hospital of Foshan City Foshan Guangdong 528010 China Department of Obstetrics and Gynecology Maternal and Child Health Hospital of Henan Province Zhengzhou Henan 450052 China Department of Obstetrics and Gynecology The Second Hospital of Shanxi Medical University Datong Shanxi 030001 China Department of Obstetrics and Gynecology The First Affiliated Hospital of Xinjiang Medical University Urumchi Xinjiang 830000 China Department of Obstetrics and Gynecology People's Hospital of Fujian Province Fuzhou Fujian 350000 China Department of Obstetrics and Gynecology Qinghai Red Cross Hospital Xining Qinghai 810000 China Department of Obstetrics and Gynecology Dalian Maternity Hospital Dalian Liaoning 116021 China Department of Obstetrics and Gynecology Tianjin Central Maternity Hospital Tianjin 300100 China Department of Obstetrics and Gynecology The Second Affiliated Hospital of Xi'an Jiaotong University Xi'an Shaanxi 710061 China Department of Obstetrics and Gynecology General Hospital of Ningxia Medical University Yinchuan Ningxia Hui Autonomous Region 750003 China Department of Obstetrics and Gynecology The First Affiliated Hospital of Harbin Medical University Harbin Heilongjiang 150007 China Department of Obstetrics and Gynecology The Second Hospital of Jilin University Changchun Jilin 130041 China Department of Obstetrics and Gynecology Shanghai Tongji Hospital Shanghai 200065 China Department of Obstetrics and Gynec

Background： The Food and Drug Administration recently announced that the use of morcellation may cause fibroids or pelvic dissemination and metastasis of uterine sarcoma; therefore, the use of morcellation is limited in the USA. A large sample study is necessary to assess the proportion of uterine malignant tumors found in patients with laparoscopic myomectomy. Methods： A national multicenter study was performed in China. From 2002 to 2014, 33,723 cases were retrospectively selected. We calculated the prevalence and recorded the clinical characteristics of the patients with malignancy after morcellation application. A total of 62 cases were finally pathologically confirmed as malignant postoperatively. Additionally, the medical records of the 62 patients were analyzed in details. Results： The proportion of postoperative malignancy after morcellation application was 0.18% （62/33,723） for patients who underwent laparoscopic myomectomy. Nearly 62.9% （39/62） of patients had demonstrated blood flow signals in the uterine fibroids before surgery. And, 23 （37.1%） patients showed rapid growth at the final preoperative ultrasound. With respect to the pathological types, 38 （61.3%） patients had detectable endometrial stromal sarcoma, 13 （21.0%） had detectable uterine leiomyosarcoma, only 3 （3.2%） had detectable carcinosarcoma, and 5 （8.1%） patients with leiomyoma had an undetermined malignant potential. Conclusions： The proportion of malignancy is low after using moreellation in patients who undergo laparoscopie myomectomy. Patients with fast-growing uterine fibroids and abnormal ultrasonic tumor blood flow should be considered for malignant potential, and morcellation should be avoided.

关键词： Laparoscopic Myomectomy Morcellation Sarcoma