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The Crop Journal 2024年第2期12卷 456-469页

作者： Jingli Ding chenchen Ji Lu yu Chuang Wang Guangda Ding Sheliang Wang Lei Shi Fangsen Xu Hongmei Cai Research Center of Microelement Huazhong Agricultural UniversityWuhan 430070HubeiChina College of Resources and Environment Huazhong Agricultural UniversityWuhan 430070HubeiChina National Key Laboratory of Crop Genetics and Improvement Huazhong Agricultural UniversityWuhan 430070HubeiChina

Transcription factors regulating crop uptake and translocation of the micronutrient Cu have not been identified.We isolated a novel R2R3-MYB transcription factor,OsMYB84,and showed that it was a positive regulator involved in uptake and transport of Cu via activation of OsCOPT2 and OsHMA expression.OsMYB84 was highly expressed in roots and anthers and induced by Cu.Overexpression of OsMYB84 promoted uptake and root-to-shoot translocation of Cu in rice,facilitated Cu distribution into grain and increased grain yield.In contrast,mutation of OsMYB84 reduced Cu concentration in xylem sap.OsMYB84 bound to the promoter region of OsCOPT2 and OsHMA5 and upregulated their expression.OsCOPT2 mutants showed reduced uptake of Cu and OsHMA5 overexpression lines showed increased root-to-shoot translocation of Cu.

关键词： OsMYB84 OsCOPT2 OsHMA5 Copper Rice

Unraveling the Rheology of Nanocellulose Aqueous Suspensions:A Comprehensive Study on Biomass-Derived Nanofibrillated Cellulose

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Journal of Renewable Materials 2024年第3期12卷 443-455页

作者： Mingyue Miao Fei Wang Qing Li Longchen Tao chenchen Dai yu Liu Shujuan Han Wenshuai Chen Ping Lu Key Laboratory of Bio-Based Material Science and Technology Ministry of EducationNortheast Forestry UniversityHarbin150040China Department of Chemistry and Biochemistry Rowan UniversityGlassboroNew Jersey08028USA

The rheological properties of nanocellulose aqueous suspensions play a critical role in the development of nanocellulose-based bulk materials.High-crystalline,high-aspect ratio,and slender nanofibrillated cellulose(NFC)were extracted from four biomass resources.The cellulose nanofibrils and nanofibril bundles formed inter-connected networks in the NFC aqueous suspensions.The storage moduli of the suspensions with different concentrations were higher than their corresponding loss moduli.As the concentration increased,the storage and loss modulus of NFC dispersion increased.When the shear rate increased to a certain value,there were differences in the changing trend of the rheological behavior of NFC aqueous suspensions derived from different biomass resources and the suspensions with different solid concentrations.NFC dispersion’s storage and loss modulus increased when the temperature rose to nearly 80℃.We hope this study can deepen the understanding of the rheological properties of NFC colloids derived from different biomass resources.

关键词： Biomass resources nanofibrillated cellulose high-pressure homogenizer suspension rheology properties

Indoor environment of nearly zero energy residential buildings with conventional air conditioning in hot-summer and cold-winter zone

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Energy and Built Environment

Energy and Built Environment 2022年第2期3卷 129-138页

作者： Jiankai Dong Haixia Lan yu Liu Xiaotao Wang chenchen yu School of Architecture Harbin Institute of TechnologyHarbinChina Key Laboratory of Cold Region Urban and Rural Human Settlement Environment Science and Technology Ministry of Industry and Information TechnologyChina CAS Key Laboratory of Cryogenics Technical Institute of Physics and ChemistryChina

According to the few researches on Nearly zero energy residential buildings(NZERB)in hot-summer and cold-winter zone,although it could reduce the cooling load of buildings due to its high thermal insulation and air tightness,it still needed for certain cooling in summer.This paper studied indoor environment of NZERB un-der three kinds of air-conditioners(split-type air-conditioner,multi-line air-conditioner and ceiling radiant air-conditioner).Firstly,a simulation model of NZERB was established based on Nanjing,a typical city in hot-summer and cold-winter zone.Secondly,variation of indoor air temperature and building load characteristics with outdoor air temperature were studied.Thirdly,indoor environment and energy consumption under three selected con-ventional air-conditioners in summer were simulated.Finally,the discussion was given,and an air-conditioner combining with convective and radiant cooling were proposed.The results indicated that the air-conditioner needed to be turned on in NZERB in hot-summer and cold-winter zone due to the room air temperature in off-air condition ranged from 32℃to 36℃,which was higher than designed indoor environment temperature in sum-mer,but the indoor environment of NZERB under three selected conventional air-conditioners could not meet the requirements of energy saving and comfort at the same time,and a proposed convective-radiant air-conditioner could be fast,stable,and energy saving.The findings can provide a reference for conducting active technology in NZERB.

关键词： Nearly zero energy residential building Load characteristics Air-conditioners Indoor environment Hot-summer and cold-winter zone

LncRNA IDH1-AS1 sponges miR-518c-5p to suppress proliferation of epithelial ovarian cancer cell by targeting RMB47

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The Journal of Biomedical Research 2024年第1期38卷 51-65页

作者： Juan Zhou Yiran Xu Luyao Wang yu Cong Ke Huang Xinxing Pan Guangquan Liu Wenqu Li chenchen Dai Pengfei Xu Xuemei Jia Department of Gynecology Women's Hospital of Nanjing Medical UniversityNanjing Maternity and Child Health Care HospitalNanjingJiangsu 210004China Nanjing Maternity and Child Health Medical Institute Women's Hospital of Nanjing Medical UniversityNanjing Maternity and Child Health Care HospitalNanjingJiangsu 210004China.

Long noncoding RNA(lncRNA)IDH1 antisense RNA 1(IDH1-AS1)is involved in the progression of multiple cancers,but its role in epithelial ovarian cancer(EOC)is unknown.Therefore,we investigated the expression levels of IDH1-AS1 in EOC cells and normal ovarian epithelial cells by quantitative real-time PCR(qPCR).We first evaluated the effects of IDH1-AS1 on the proliferation,migration,and invasion of EOC cells through cell counting kit-8,colony formation,EdU,transwell,wound-healing,and xenograft assays.We then explored the downstream targets of IDH1-AS1 and verified the results by a dual-luciferase reporter,qPCR,rescue experiments,and Western blotting.We found that the expression levels of IDH1-AS1 were lower in EOC cells than in normal ovarian epithelial cells.High IDH1-AS1 expression of EOC patients from the Gene Expression Profiling Interactive Analysis database indicated a favorable prognosis,because IDH1-AS1 inhibited cell proliferation and xenograft tumor growth of EOC.IDH1-AS1 sponged miR-518c-5p whose overexpression promoted EOC cell proliferation.The miR-518c-5p mimic also reversed the proliferation-inhibiting effect induced by IDH1-AS1 overexpression.Furthermore,we found that RNA binding motif protein 47(RBM47)was the downstream target of miR-518c-5p,that upregulation of RBM47 inhibited EOC cell proliferation,and that RBM47 overexpressing plasmid counteracted the proliferation-promoting effect caused by the IDH1-AS1 knockdown.Taken together,IDH1-AS1 may suppress EOC cell proliferation and tumor growth via the miR-518c-5p/RBM47 axis.

关键词： lncRNA IDH1-AS1 epithelial ovarian cancer miR-518c-5p RBM47

Effects of plant diversity on greenhouse gas emissions in microcosms simulating vertical constructed wetlands with high ammonium loading

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Journal of Environmental Sciences 2019年第3期31卷 229-237页

作者： Wenjuan Han Guiying Luo Bin Luo chenchen yu Hai Wang Jie Chang Ying Ge College of Life Sciences Zhejiang University College of Chemistry and Life Sciences Zhejiang Normal University College of Life Sciences Shaoxing University

Wastewater with relatively high nitrogen concentrations is a major source of nitrous oxide(N_2O) and methane(CH_4) emissions and exerts multiple stresses on the environment.Studies have shown that plant diversity plays an important role in ecosystem functioning.However, the effects of plant species diversity on CH_4 and N_2O emissions under high ammonium(NH_4^+-N) loading rates remain unclear. In this study, a microcosm experiment simulating vertical constructed wetlands supplied with high NH_4^+-N water levels was established. The treatments included four species richness levels(1, 2, 3, 4) and 15 species compositions. There was no significant relationship between species richness and N_2O emissions. However, N_2O emissions were significantly reduced by specific plant species composition. Notably, the communities with the presence of Rumex japonicus L. reduced N_2O emissions by 62% compared to communities without this species. This reduction in N_2O emissions may have been a result of decreased N concentrations and increased plant biomass. CH_4 emissions did not respond to plant species richness or species identity.Overall, plant species identity surpassed species richness in lowering N_2O emissions from constructed wetlands with high NH_4^+-N water. The results also suggest that communities with R. japonicus could achieve higher N removal and lower greenhouse gas emissions than other wetland species.

关键词： Plant species richness Species identity Methane Nitrous oxide Nitrogen removal Ecosystem functioning

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改进的YOLOv4头盔佩戴目标检测研究

沈阳工程学院学报（自然科学版） 2024年第1期20卷 75-81页

作者：余晨晨安徽理工大学机械工程学院安徽淮南232001

针对骑手在骑行时是否佩戴头盔对交通安全的影响问题,提出了一种改进的YOLOv4算法,能够更准确地识别和检测骑手是否佩戴头盔,从而为骑手提供安全保障。首先,选择轻量级网络MobileNetv1作为主干特征网络,并将YOLOv4网络中尺寸为3×3、步... 详细信息

针对骑手在骑行时是否佩戴头盔对交通安全的影响问题,提出了一种改进的YOLOv4算法,能够更准确地识别和检测骑手是否佩戴头盔,从而为骑手提供安全保障。首先,选择轻量级网络MobileNetv1作为主干特征网络,并将YOLOv4网络中尺寸为3×3、步长为1的标准卷积层均替换为深度可分离卷积,减少模型计算量的同时提升检测速度;其次,引入ECA注意力机制,关注重点特征并抑制非必要特征,增加特征网络表现力;最后,引入改进的损失函数Focal-EIOU,改善常见的样本不均衡问题。实验结果表明:改进的YOLOv4算法生成的模型权重大小为48.43 M,是YOLOv4算法权重大小的19.3%,检测速度由33.40帧/秒提升至50.40帧/秒,mAP值为95.56%,在满足精确性的前提下更有利于轻量化部署。

关键词： YOLOv4 目标检测 MobileNetv1 ECA注意力机制 Focal-EIOU

镍锰氧化物复合电化学传感器对抗坏血酸的检测

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精细化工 2024年第5期41卷 1022-1029,1035页

作者：韩春然岳振歌遇世友王鑫黎晨晨张丝瑶哈尔滨商业大学食品工程学院黑龙江哈尔滨150028

首先,通过一步水热法制备了镍锰氧化物复合材料(Mn-Ni-Oxide),并将其修饰玻碳电极构建抗坏血酸快速检测的电化学传感器。利用XRD、SEM、EDS及FTIR对Mn-Ni-Oxide进行了表征。采用滴涂法将Mn-Ni-Oxide修饰于电极表面,并用循环伏安(CV)法... 详细信息

首先,通过一步水热法制备了镍锰氧化物复合材料(Mn-Ni-Oxide),并将其修饰玻碳电极构建抗坏血酸快速检测的电化学传感器。利用XRD、SEM、EDS及FTIR对Mn-Ni-Oxide进行了表征。采用滴涂法将Mn-Ni-Oxide修饰于电极表面,并用循环伏安(CV)法、差分脉冲伏安(DPV)法测试了抗坏血酸在Mn-Ni-Oxide修饰电极上的电化学行为。结果表明,Mn-Ni-Oxide的外表面为带有孔隙的纳米球形结构,主要组分为Mn_(2)O_(3)、NiMnO_(3)。在pH为4的乙酸/乙酸钠缓冲溶液条件下,修饰电极的Mn-Ni-Oxide修饰量为10μL,抗坏血酸浓度(c)在0.1~9000μmol/L时与氧化峰电流(I_(p))呈线性关系,线性方程为I_(p)=3.2435c+17.198(R^(2)=0.9920),检出限为0.025μmol/L,灵敏度为3.2435μA·m·L/(mol·cm)。该传感器实际应用于果汁饮料中抗坏血酸的测定,加标回收率为95.5%~103.1%,表明其在食品分析等方面具有良好的应用前景。

关键词：抗坏血酸镍锰氧化物复合材料电化学传感器功能材料

Maillard reaction affecting immunobinding activity and digestibility of tropomyosin in Alectryonella plicatula food matrix

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Food Science and Human Wellness 2023年

作者： chenchen yu Xinrong He Nairu Ji Shuai Kang Dongxiao Li Huilin Zhang Yang Yang Dong Lai Qingmei Liu Guangming Liu College of Ocean Food and Biological Engineering Xiamen Key Laboratory of Marine Functional Food Fujian Provincial Engineering Technology Research Center of Marine Functional Food Jimei University The Second Affiliated Hospital of Xiamen Medical College

In recent years， the allergy rate of oysters has surged， and daily food processing methods make it hard to reduce heat resistance and digestive allergy such as tropomyosin (TM). In this study， the Maillard reaction with xylose significantly reduced the IgE binding capacity of Alectryonella plicatula food matrix (AFM)， that reduced by (77.81 ± 2.68)%. The study found the Maillard reaction changes the structure of the AFM， in which the content of α-helix decreased by (24.64 ± 1.46)%. Structural transformation further explains why the Maillard reaction alters the immunobinding activity of AFM. In addition， the Maillard reaction reduces the digestive stability of the AFM and makes tropomyosin (TM) in the A. plicatula food matrix Maillard reaction products (AFM-MRPs) more easily digested. Based on the above research， 10 amino acids on the 7 IgE epitopes of TM were modified. This result indicates that the Maillard reaction reduces the immunobinding activity of the AFM by changing the structure and modifying the amino acids on the epitope.

关键词：

蛇床子素通过NF-kB信号通路调控退变髓核细胞的细胞外基质表达、炎症反应及凋亡

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中华中医药学刊 2024年第3期42卷 142-146页

作者：高峰于喜微李晨辰锦州医科大学附属第三医院辽宁锦州121000

目的研究蛇床子素对退变髓核细胞的细胞外基质表达、炎症反应和凋亡的作用及其潜在机制。方法采用氧糖剥夺(Oxygen Glucose Deprivation, OGD)培养建立退变髓核细胞模型,将细胞分为:对照组(正常培养)、OGD组(OGD培养)、蛇床子素组(OG... 详细信息

目的研究蛇床子素对退变髓核细胞的细胞外基质表达、炎症反应和凋亡的作用及其潜在机制。方法采用氧糖剥夺(Oxygen Glucose Deprivation, OGD)培养建立退变髓核细胞模型,将细胞分为:对照组(正常培养)、OGD组(OGD培养)、蛇床子素组(OGD培养+蛇床子素处理)、PDTC组(OGD培养+NF-κB通路抑制剂PDTC处理)和蛇床子素+PDTC组(OGD培养+蛇床子素+PDTC处理)。采用Western blot检测细胞中Ⅱ型胶原(Collagen typeⅡ,Col2al)、聚集蛋白聚糖(Aggrecan)、P65和p-P65蛋白表达,ELISA法检测细胞培养液中炎性因子血清白细胞介素-1β(interleukin-1 β,IL-1β),白细胞介素-6(interleukin-6,IL-6)和肿瘤坏死因子-α(Tumor necrosis factor-α,TNF-α)的水平,流式细胞仪检测细胞凋亡率。结果与对照组比较,OGD组细胞中Col2al、Aggrecan蛋白相对表达水平明显降低,细胞培养液中IL-1β、IL-6和TNF-α水平升高,细胞凋亡率明显升高,细胞中p-P65蛋白表达明显升高;与OGD组比较,蛇床子素组和PDTC组细胞中Col2al、Aggrecan蛋白相对表达水平明显升高,细胞培养液中IL-1β、IL-6和TNF-α水平降低,细胞凋亡率明显降低,细胞中p-P65蛋白表达明显降低,而蛇床子素+PDTC组上述指标改变最为明显。结论蛇床子素可能通过抑制核因子激活的B细胞的κ轻链增强(nuclear factorκB,NF-κB)信号通路的活性,促进退变髓核细胞的细胞外基质合成、抑制退变髓核细胞的炎症反应和凋亡。

关键词：髓核细胞蛇床子素 NF-kB信号通路细胞外基质炎症反应凋亡

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气基竖炉直接还原数值模拟分析

钢铁 2024年第1期59卷 41-48页

作者：白晨晨师学峰王明阳于浩胡长庆韩涛华北理工大学冶金与能源学院河北唐山063210

针对气基竖炉直接还原过程无法直接观察还原反应运行程度的难点,基于三界面未反应核模型,在忽略模型球团内部温度及假设球团还原反应的热效应完全发生在固相的条件下,建立了气基竖炉直接还原模型,对铁氧化物价态转变进行了数值模拟和验... 详细信息

针对气基竖炉直接还原过程无法直接观察还原反应运行程度的难点,基于三界面未反应核模型,在忽略模型球团内部温度及假设球团还原反应的热效应完全发生在固相的条件下,建立了气基竖炉直接还原模型,对铁氧化物价态转变进行了数值模拟和验证。结果表明,由于所建立的气固模型包含3个界面,随着铁氧化物的逐级还原,每个界面的反应半径最终趋于0,而还原反应速率随着竖炉深度的增加呈现出先升高后降低的趋势。球团在竖炉内下降到3 m深度时,出现半径为15 mm的FeO反应界面,此时球团还原率约为28%。随着球团继续在竖炉内下行约2 m到达5 m的深度时,Fe_(3)O_(4)的界面半径减小为0,此时铁氧化物完全转变成了浮氏体形态,球团还原率约为34%。通过改变不同的工艺参数进行模拟可以发现,还原球团金属化率和还原率随着气体温度的升高而增大。当气体温度以50℃、还原气体流量以5040 m^(3)/h梯度增大时,其对应的球团金属化率分别增大8%和4%左右。相比之下,球团金属化率受下料速度的影响远超过气体温度和还原气体流量,具体表现为,当下料速度增大0.02 t/h时,金属化率降低约0.07%。而当球团粒度减小时,金属化率会表现增大的趋势,球团粒度减小1 mm,球团金属化率增大2.23%。

关键词：气基竖炉气基法直接还原数值模拟还原率金属化率