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Chinese Medical Journal 2022年第1期135卷 95-97页

作者： Yanan Yu Miaorong Xie Jiabao Li Chenchen Hang Fei Shao Chunsheng Li Department of Emergency Medicine Beijing Friendship HospitalCapital Medical UniversityBeijing 100020China Department of Critical Care Medicine Beijing Friendship HospitalCapital Medical UniversityBeijing 100020China Department of Emergency Medicine Beijing Chaoyang HospitalCapital Medical UniversityBeijing 100020China

To the Editor:Cardiac arrest(CA)is the leading cause of morbidity and mortality among hospitalized patients globally and has low rates of overall survival.After the return of spontaneous circulation(ROSC),patients with CA and systemic ischemia/reperfusion injury can manifest as sepsis-like syndromes and immune disorders that increase the risk of infections.CD4^(+)T lymphocytes play an important role in organ ischemia-reperfusion injury.T-helper(Th)types 1 and 2 cells are classical subsets of CD4^(+)T cells.Extensive research on their functions in infectious and immune-related inflammatory diseases indicates that both subsets participate in host defense and direct immune responses.Data from CA/cardiopulmonary resuscitation porcine models and patients have revealed imbalances in Th1/Th2 cell ratio.[1]Therefore,Th1 and Th2 cells are important for understanding post-ROSC immune dysfunction in patients with CA.

关键词： Th1/Th2 patients Th2

Enhanced tissue regeneration through immunomodulation of angiogenesis and osteogenesis with a multifaceted nanohybrid modified bioactive scaffold

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Bioactive Materials 2022年第12期7卷 552-568页

作者： hang Xue Zhenhe Zhang Ze Lin Jin Su Adriana CPanayi Yuan Xiong Liangcong Hu Yiqiang Hu Lang Chen chenchen Yan Xudong Xie Yusheng Shi Wu Zhou Bobin Mi Guohui Liu Department of Orthopaedics Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430022China Hubei Province Key Laboratory of Oral and Maxillofacial Development and Regeneration Wuhan430022China State Key Laboratory of Materials Processing and Die&Mould Technology School of Materials Science and EngineeringHuazhong University of Science and TechnologyWuhan430074China Division of Plastic Surgery Brigham and Women’s HospitalHarvard Medical SchoolBostonMA02215USA

Major traumatic tissue defects are common clinical problems often complicated by infection and local vascular dysfunction, processes which hinder the healing process. Although local application of growth factors or stem cells through various tissue engineering techniques are promising methods for the repair of tissue defects, limitations in their clinical application exist. Herein, we synthesized multifaceted nanohybrids composed of Quaternized chitosan (QCS), Graphene oxide (GO), and Polydopamine (PDA;QCS-GO-PDA). Covalent grafting of QCS and GO at a mass ratio of 5:1 (5QCS-1GO) displayed excellent biocompatibility and enhanced osteogenic ability, while addition of PDA (5QCS-1GO-PDA) reduced the level of reactive oxygen species (ROS). 5QCS-1GO-PDA was able to achieve wound tissue regeneration by reducing the inflammatory response and enhancing angiogenesis. Furthermore, Polylactic acid/hydroxyapatite (PLA/HA) composite scaffolds were printed using Selective Laser Sintering (SLS) and the hybrid nanomaterial (5QCS-1GO-PDA) was used to coat the PLA/HA scaffold (5QCS-1GO-PDA@PLA/HA) to be used for rapid bone regeneration. 5QCS-1GO-PDA not only improved angiogenesis and osteogenic differentiation, but also induced M2-type polarization of macrophages and promoted bone regeneration via the BMP2/BMPRs/Smads/Runx2 signaling pathway. The bidirectional enhanced healing ability of the multifaceted nanohybrids 5QCS-1GO-PDA provides a promising method of effectively treating tissue defects.

关键词： Multifunctional nanohybrids Quaternary chitosan Polydopamine Angiogenesis Tissue regeneration

Bone-targeting engineered small extracellular vesicles carrying anti-miR-6359-CGGGAGC prevent valproic acid-induced bone loss

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Signal Transduction and Targeted Therapy 2024年第2期9卷 751-765页

作者： Xudong Xie Peng Cheng Liangcong Hu Wu Zhou Detai Zhang Samuel Knoedler Guodong Liu Yuan Xiong hang Xue Yiqiang Hu Barbara Kern Doha Obed Adriana CPanayi Lang Chen chenchen Yan Ze Lin Guandong Dai Bobin Mi Yingze Zhang Guohui Liu Department of Orthopedics Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Hubei Province Key Laboratory of Oral and Maxillofacial Development and Regeneration Wuhan 430022China Department of Clinical Laboratory Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei 430022P.R.China Division of Plastic Surgery Brigham and Women’s HospitalHarvard Medical SchoolBostonMA 02152USA Department of Plastic Surgery and Hand Surgery Klinikum Rechts der IsarTechnical University of MunichMunichGermany Medical Center of Trauma and War Injuries Daping HospitalArmy Medical UniversityChonqing 400042China Department of Plastic Surgery Campus CharitéMitte|Campus Virchow-KlinikumCharité-Universitätsmedizin Berlincorporate member of Freie Universität BerlinHumboldt-Universität zu Berlin and Berlin Institute of HealthBerlinGermany Berlin Institute of Health Charité-Universitätsmedizin BerlinBerlinGermany Department of Plastic AestheticHand and Reconstructive SurgeryHannover Medical SchoolHannoverGermany Department of Hand Plastic and Reconstructive SurgeryMicrosurgeryBurn CenterBG Trauma Center LudwigshafenUniversity of HeidelbergLudwig-Guttmann-Strasse 1367071 Ludwigshafen/RhineGermany Pingshan District People’s Hospital of Shenzhen Pingshan General Hospital of Southern Medical UniversityShenzhenGuangdong 518118China Department of Orthopaedic Surgery The Third Hospital of Hebei Medical UniversityNO.139 Ziqiang RoadShijiazhuang 050051China

The clinical role and underlying mechanisms of valproic acid(VPA)on bone homeostasis remain controversial.Herein,we confirmed that VPA treatment was associated with decreased bone mass and bone mineral density(BMD)in both patients and mice.This effect was attributed to VPA-induced elevation in osteoclast formation and activity.Through RNA-sequencing,we observed a significant rise in precursor miR-6359 expression in VPA-treated osteoclast precursors in vitro,and further,a marked upregulation of mature miR-6359(miR-6359)in vivo was demonstrated using quantitative real-time PCR(qRT-PCR)and miR-6359 fluorescent in situ hybridization(miR-6359-FISH).Specifically,the miR-6359 was predominantly increased in osteoclast precursors and macrophages but not in neutrophils,T lymphocytes,monocytes and bone marrow-derived mesenchymal stem cells(BMSCs)following VPA stimulation,which influenced osteoclast differentiation and bone-resorptive activity.Additionally,VPA-induced miR-6359 enrichment in osteoclast precursors enhanced reactive oxygen species(ROS)production by silencing the SIRT3 protein expression,followed by activation of the MAPK signaling pathway,which enhanced osteoclast formation and activity,thereby accelerating bone loss.Currently,there are no medications that can effectively treat VPA-induced bone loss.Therefore,we constructed engineered small extracellular vesicles(E-sEVs)targeting osteoclast precursors in bone and naturally carrying anti-miR-6359 by introducing of EXOmotif(CGGGAGC)in the 3’-end of the anti-miR-6359 sequence.We confirmed that the E-sEVs exhibited decent bone/osteoclast precursor targeting and exerted protective therapeutic effects on VPA-induced bone loss,but not on ovariectomy(OVX)and glucocorticoid-induced osteoporotic models,deepening our understanding of the underlying mechanism and treatment strategies for VPA-induced bone loss.

关键词： miR protective stimulation

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基于图网络融合的交通状态预测方法研究

武汉理工大学学报（交通科学与工程版） 2022年第2期46卷 195-200页

作者：徐东伟商学天魏臣臣彭航浙江工业大学网络空间安全研究院杭州310023 浙江工业大学信息工程学院杭州310023

文中考虑道路节点之间的时间相关性,利用皮尔逊相关性系数构建逻辑相关路网;通过图聚合算法聚合道路节点邻居信息,融合原始交通路网与逻辑相关路网提取的时空特征信息,以最小化损失函数为目标,返回最优模型参数,构建基于图网络融合的交... 详细信息

文中考虑道路节点之间的时间相关性,利用皮尔逊相关性系数构建逻辑相关路网;通过图聚合算法聚合道路节点邻居信息,融合原始交通路网与逻辑相关路网提取的时空特征信息,以最小化损失函数为目标,返回最优模型参数,构建基于图网络融合的交通路网模型.采用西雅图高速路网速度数据集(seattle)和加州流量数据集(PEMS08)作试验验证,图网络融合模型提高了在交通状态预测精度.在短时交通状态预测中,Seattle的MAE指标为2.57、MAPE指标为6.48;PEMS08的MAE指标为14.23、MAPE指标为7.15;长时交通状态预测结果均优于LSTM、T-GCN等模型.

关键词：智能交通交通流预测图网络路网交通状态数据特征融合相关性网络

Exosomal PD-L1 induces osteogenic differentiation and promotes fracture healing by acting as an immunosuppressant

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Bioactive Materials 2022年第7期7卷 300-311页

作者： Ze Lin Yuan Xiong Weilin Meng Yiqiang Hu Lili Chen Lang Chen hang Xue Adriana CPanayi Wu Zhou Yun Sun Faqi Cao Guodong Liu Liangcong Hu chenchen Yan Xudong Xie Chuanchuan Lin Kaiyong Cai Qian Feng Bobin Mi Guohui Liu Department of Orthopaedics Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430022China Hubei Province Key Laboratory of Oral and Maxillofacial Development and Regeneration Wuhan430022China Key Laboratory of Biorheological Science and Technology Ministry of EducationCollege of BioengineeringChongqing UniversityChongqing400044China Division of Plastic Surgery Brigham and Women’s HospitalHarvard Medical SchoolBostonMA02152USA Department of Neurosurgery Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430022China Medical Center of Trauma and War Injuries Daping HospitalArmy Medical UniversityChongqing400042China Department of Stomatology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430022China

A moderate inflammatory response at the early stages of fracture healing is necessary for callus formation.Over-active and continuous inflammation,however,impairs fracture healing and leads to excessive tissue damage.Adequate fracture healing could be promoted through suppression of local over-active immune cells in the fracture site.In the present study,we achieved an enriched concentration of PD-L1 from exosomes(Exos)of a genetically engineered Human Umbilical Vein Endothelial Cell(HUVECs),and demonstrated that exosomes overexpressing PD-L1 specifically bind to PD-1 on the T cell surface,suppressing the activation of T cells.Furthermore,exosomal PD-L1 induced Mesenchymal Stem Cells(MSCs)towards osteogenic differentiation when pre-cultured with T cells.Moreover,embedding of Exos into an injectable hydrogel allowed Exos delivery to the surrounding microenvironment in a time-released manner.Additionally,exosomal PD-L1,embedded in a hydrogel,markedly promoted callus formation and fracture healing in a murine model at the early over-active inflammation phase.Importantly,our results suggested that activation of T cells in the peripheral lymphatic tissues was inhibited after local administration of PD-L1-enriched Exos to the fracture sites,while T cells in distant immune organs such as the spleen were not affected.In summary,this study provides the first example of using PD-L1-enriched Exos for bone fracture repair,and highlights the potential of Hydrogel@Exos systems for bone fracture therapy through immune inhibitory effects.

关键词： PD-L1 Exosome Fracture healing Hydrogel Immunotherapy

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急诊医学临床教学与规培方法探索

中国病案 2023年第10期24卷 78-81页

作者：王涛杭晨晨唐子人首都医科大学附属北京朝阳医院急诊科北京市100020

急诊医学作为一门跨专业跨学科的综合性学科,患者病情多急、重、复杂,较多病情隐匿,变化迅速,涉及疾病谱广,医师承担风险极大,急诊医师的培养应当着重于培养扎实、涉及多学科的医学理论知识和临床技能,使其具备独立、规范地承担常见多... 详细信息

急诊医学作为一门跨专业跨学科的综合性学科,患者病情多急、重、复杂,较多病情隐匿,变化迅速,涉及疾病谱广,医师承担风险极大,急诊医师的培养应当着重于培养扎实、涉及多学科的医学理论知识和临床技能,使其具备独立、规范地承担常见多发疾病诊疗工作的能力。急诊医师教师梯队资源薄弱,多数带教教师往往同时担负着诊疗工作,现场带教时由于患者病情急且复杂,环境差,自身工作强度大,既达不到预期带教效果,还容易存在医疗隐患。针对急诊医疗的教学特点,注重团队合作能力的培训,构建和谐安全的医疗环境、强化理论与操作并行、因材施教、加强医患沟通与人文医学教学、重视综合素质培养、充分利用新科技进行多元化教学以及加强教师队伍建设,以完善临床教学及规培体系,培养更加优秀、全面的临床医师。

关键词：急诊医学临床教学住院医师规范化培训医患沟通综合素质

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基于热成像的机房热点成因自动诊断方法

计算机测量与控制 2020年第4期28卷 66-70页

作者：刘航鲍晨晨谢婷高山大连理工大学电子信息与电气工程学部辽宁大连116023 大连理工大学辽宁省集成电路与生物医学电子系统重点实验室辽宁大连116023 大连理工大学网络与信息化中心辽宁大连116023

服务器设备的异常高温在机房内部会形成热点,不仅会影响服务器的稳定和寿命,还会导致机房制冷效率的降低,从而增加机房的制冷能耗,增加运营费用;导致产生热点的原因有很多,例如空气流通不畅、风扇失灵、长时间满负荷运行等等;通过自动... 详细信息

服务器设备的异常高温在机房内部会形成热点,不仅会影响服务器的稳定和寿命,还会导致机房制冷效率的降低,从而增加机房的制冷能耗,增加运营费用;导致产生热点的原因有很多,例如空气流通不畅、风扇失灵、长时间满负荷运行等等;通过自动诊断热点的成因,可以有针对性的消除热点,为机房环境控制提供数据支持,有助于降低机房制冷能耗;根据热像仪拍摄的服务器出风口一侧的红外图像,利用人工智能技术,提出了自动诊断热点成因的方法;针对实际工程应用中热点样本数量不足的问题,提出了基于深度卷积对抗生成网络(DCGAN)合成热点样本的解决方案;通过多组实验验证了方法的有效性,热点成因的诊断准确率约为95%。

关键词：信息处理技术自动诊断热成像对抗生成网络卷积神经网络

吡唑并[1,5-a]吲哚类化合物的合成及其初步抗肿瘤活性的研究

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苏州大学学报（自然科学版） 2010年第2期26卷 73-75页

作者：杭臣臣朱永明苏州大学药学院江苏苏州215123

以取代的邻溴苯乙酸为原料,经过一系列转化得到吡唑类化合物,再经Cu(I)催化下的分子内氨基化反应得到吡唑并[1,5-a]吲哚结构的中间体,最终得到吡唑并[1,5-a]吲哚类目标衍生物5 a、5b、5 c、5d,并采用MTT法评价目标物5的抗肿瘤活性.

关键词：吡唑并[1,5-a]吲哚类衍生物合成抗肿瘤

一株有色金属矿山1-亚硝基-2-萘酚降解菌的筛选、鉴定和降解特性

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微生物学报 2022年第11期62卷 4385-4396页

作者：李欣媛孟行姚俊李浩朱潇哲赵陈晨刘建丽中国地质大学(北京)水资源与环境学院北京100083

1-亚硝基-2-萘酚是一类有色选矿药剂代表性的新型浮选药剂,在采选冶行业为了提高低品位矿物资源的利用率,被大量投入使用,该药剂的高稳定性进一步增大了矿山环境中重金属与有机选冶药剂复合污染的治理难度。微生物修复作为稠环芳烃(poly... 详细信息

1-亚硝基-2-萘酚是一类有色选矿药剂代表性的新型浮选药剂,在采选冶行业为了提高低品位矿物资源的利用率,被大量投入使用,该药剂的高稳定性进一步增大了矿山环境中重金属与有机选冶药剂复合污染的治理难度。微生物修复作为稠环芳烃(polycyclic aromatic hydrocarbons,PAHs)类污染物重要技术手段之一,具有安全、经济、高效和无二次污染等特点。【目的】本研究从我国广西河池市周边的典型有色金属尾矿环境中分离出1株高效降解1-亚硝基-2-萘酚的菌株,并分析其降解特性及其潜在的代谢途径,从而探究矿山复合污染生态系统中稠环芳烃类污染物的微生物修复技术的应用前景。【方法】从有色金属尾矿样本中筛选到以1-亚硝基-2-萘酚为唯一碳源的菌株,经16S rRNA基因序列鉴定,结合气相色谱-质谱联合(gas chromatography-mass spectrometer,GC-MS)检测分析菌株对1-亚硝基-2-萘酚的降解特性及中间代谢产物,并推测可能的代谢途径。【结果】筛选获得1株高效降解1-亚硝基-2-萘酚的Pseudomonas putida CUGB-JL11菌株,经鉴定为革兰氏阴性杆的恶臭假单胞菌。该菌株最适温度为30℃左右,最适pH值范围为6-8条件下,菌株5 d内对40 mg/L 1-亚硝基-2-萘酚的降解率高达81%。降解中间代谢产物主要为苯环类物质:苯甲羟肟酸甲酯和苯丙胺,但其母体及大部分中间产物都降解为小分子物质或者被完全降解。【结论】恶臭假单胞菌P. putida CUGB-JL11具有良好的1-亚硝基-2-萘酚降解能力和较强的环境适应性,有进一步被开发为微生物菌剂以用于稠环芳烃类污染修复的巨大潜力,为有色金属矿山生态系统中重金属和有机选冶药剂复合污染的微生物修复研究提供了理论依据和可利用的微生物资源。

关键词：有色金属选冶渣场 1-亚硝基-2-萘酚降解菌分子鉴定气相色谱-质谱(GC-MS)

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基于支持向量机的时深建模方法

大庆石油地质与开发 2018年第3期37卷 153-157页

作者：袁航谢玮毕臣臣岳占伟刘伟刘学清中国地质大学(北京)地球物理与信息技术学院北京100083 北京京能油气资源开发有限公司北京100022

为了解决海上勘探初期地震资料解释过程中的时深关系问题,提出基于粒子群和支持向量机的时深建模方法,利用粒子群算法来优化支持向量机的参数。首先由合成地震记录标定得到每口井的时深关系;然后利用支持向量机方法建立时间与深度之间... 详细信息

为了解决海上勘探初期地震资料解释过程中的时深关系问题,提出基于粒子群和支持向量机的时深建模方法,利用粒子群算法来优化支持向量机的参数。首先由合成地震记录标定得到每口井的时深关系;然后利用支持向量机方法建立时间与深度之间的关系模型;最后以此模型对时间域等T0图进行转换,从而得到深度域的构造图。实际测井数据和三维地震资料试验结果表明,该方法建立的时深关系模型适用于整个研究区,且时间域转换深度与井分层深度、构造深度之间的误差较小,能够满足精细构造解释的精度要求。

关键词：地震资料解释粒子群算法支持向量机时深关系建模