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Loss-of-function mutations of microRNA-142-3p promote ASH1L expression to induce immune evasion and hepatocellular carcinoma progression

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World Journal of Gastroenterology 2025年第1期31卷 126-145页

作者： Xing-Hui yu Yan Xie Jian yu Kun-Ning Zhang Zhou-Bo Guo Di Wang Zhao-Xian li Wei-Qi Zhang yu-Ying Tan li Zhang Wen-Tao Jiang School of Medicine Nankai UniversityTianjin 300192China Tianjin Key Laboratory of Molecular Diagnosis and Treatment of liver Cancer Tianjin First Center HospitalTianjin 300192China Department of liver Transplantation Tianjin First Center HospitalTianjin 300192China First Central Clinical School Tianjin Medical UniversityTianjin 300192China

BACKGROUND Hepatocellular carcinoma(HCC)has been a pervasive malignancy throughout the world with elevated *** therapeutic targets are beneficial to treat and predict the ***,the exact molecular mechanisms leading to the progression of HCC are still *** has shown that the microRNA-142-3p level decreases in HCC,whereas bioinformatics analysis of the cancer genome atlas database shows the ASH1L expression increased among liver tumor *** this paper,we will explore the effects and mechanisms of microRNA-142-3p and ASH1L affect the prognosis of HCC patients and HCC cell bioactivity,and the association between *** To investigate the effects and mechanisms of microRNA-142-3p and ASH1L on the HCC cell bioactivity and prognosis of HCC *** In this study,we grouped HCC patients according to their immunohistochemistry results of ASH1L with pathological tissues,and retrospectively analyzed the prognosis of HCC ***,explored the roles and mechanisms of microRNA-142-3p and ASH1L by cellular and animal experiments,which involved the following experimental methods:Immunohistochemical staining,western blot,quantitative real-time-polymerase chain reaction,flow cytometric analysis,tumor xenografts in nude mice,*** statistical methods involved in this study contained t-test,one-way analysis of variance,theχ^(2)test,the Kaplan-Meier approach and the log-rank *** In this study,we found that HCC patients with high expression of ASH1L possess a more recurrence rate as well as a decreased overall survival ***1L promotes the tumorigenicity of HCC and microRNA-142-3p exhibits reduced expression in HCC tissues and interacts with ASH1L through targeting the ASH1L 3′untranslated ***,microRNA-142-3p promotes apoptosis and inhibits proliferation,invasion,and migration of HCC cell lines in vitro via *** the exploration mechanism,we found ASH1L may promote an immunosuppressive mi

关键词： Hepatocellular carcinoma MicroRNA-142-3p ASH1L Immune evasion Tumor immune microenvironment Apoptosis

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蓝莓花色苷对小鼠淋巴细胞和腹腔巨噬细胞的免疫调节作用

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食品工业科技 2018年第19期39卷 296-301页

作者：刘玉晨李敬双于洋锦州医科大学食品科学与工程学院辽宁锦州121001

为探讨蓝莓花色苷对淋巴细胞和腹腔巨噬细胞的免疫调节作用。无菌分离淋巴细胞和腹腔巨噬细胞,制备淋巴细胞和腹腔巨噬细胞悬液,以终浓度为4、8、16、32、64 mg/m L的蓝莓花色苷作用于体外培养的淋巴细胞(48 h)和腹腔巨噬细胞(24 h),采... 详细信息

为探讨蓝莓花色苷对淋巴细胞和腹腔巨噬细胞的免疫调节作用。无菌分离淋巴细胞和腹腔巨噬细胞,制备淋巴细胞和腹腔巨噬细胞悬液,以终浓度为4、8、16、32、64 mg/m L的蓝莓花色苷作用于体外培养的淋巴细胞(48 h)和腹腔巨噬细胞(24 h),采用MTT法检测蓝莓花色苷对淋巴细胞增殖率及中性红吞噬实验检测腹腔巨噬细胞吞噬功能的影响,EliSA法检测蓝莓花色苷对淋巴细胞(IL-6白细胞介素-6)、(TNF-α肿瘤坏死因子-α)和腹腔巨噬细胞(IL-4白细胞介素-4)、(IL-12白细胞介素-12)分泌的影响。结果显示,蓝莓花色苷各浓度组淋巴细胞增殖率、腹腔巨噬细胞的吞噬活性、培养上清中淋巴细胞IL-6、TNF-α及腹腔巨噬细胞IL-12的分泌高于空白组(p <0.05),各浓度组与阳性组腹腔巨噬细胞IL-4分泌量均低于空白对照组(p <0.05)。蓝莓花色苷可能通过促进淋巴细胞增殖和提高腹腔巨噬细胞吞噬及分泌功能,提高机体免疫功能。

关键词：蓝莓花色苷淋巴细胞增殖巨噬细胞吞噬细胞因子免疫调节

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溪黄草黄酮对肝癌细胞增殖,迁移和侵袭的影响及相关机制

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世界华人消化杂志 2018年第17期26卷 1029-1035页

作者：李晨瑜张喜红台州恩泽医疗中心(集团)恩泽医院药剂科浙江省台州市318050

目的探讨溪黄草黄酮[Flavonoids from Rabdosia serra(Maxim.)Hara]对人肝癌细胞株Hep G2的增殖、迁移与侵袭能力的影响,并研究初步机制.方法 CCK-8法检测不同浓度溪黄草黄酮(0、1、5、10、20和40μmol/L)对Hep G2细胞活性的影响;划痕... 详细信息

目的探讨溪黄草黄酮[Flavonoids from Rabdosia serra(Maxim.)Hara]对人肝癌细胞株Hep G2的增殖、迁移与侵袭能力的影响,并研究初步机制.方法 CCK-8法检测不同浓度溪黄草黄酮(0、1、5、10、20和40μmol/L)对Hep G2细胞活性的影响;划痕实验和Transwell侵袭实验分别检测细胞的迁移和侵袭能力;Western blot检测Notch-1,Cyclin D1,MMP-2和MMP-9蛋白表达水平变化.结果 CCK-8法检测结果显示溪黄草黄酮呈浓度依赖性抑制Hep G2细胞的生长,当浓度到达20μmol/L时,抑制作用达到最大;经溪黄草黄酮干预后,Hep G2细胞迁移和侵袭能力显著下降,Notch-1,Cyclin D1,MMP-2和MMP-9蛋白表达水平显著下降.结论溪黄草黄酮具有抑制肝癌细胞株Hep G2增殖、迁移和侵袭的作用,其作用机制可能与溪黄草黄酮抑制Notch-1-MMP-2/-9和Notch-1-Cyclin D1信号通路相关.

关键词：溪黄草黄酮增殖迁移侵袭肝癌细胞

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核磁共振成像评价“接力赛”电针法对腹型肥胖女性腹部脂肪的影响

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针刺研究 2016年第5期41卷 451-456页

作者：雷红陈晓胡动刚陈玉婷冯立成陈镇燕李芳华中科技大学同济医学院附属普爱医院针灸科武汉430033 华中科技大学同济医学院附属同济医院放射科武汉430000 华中农业大学理学院武汉430070 华中科技大学同济医学院附属同济医院临床营养科武汉430000 湖北省咸宁市咸安区中医院咸宁437000

目的:应用核磁共振成像(MRI)评价"接力赛"电针法对女性腹部脂肪的影响。方法:30例腹型肥胖女性随机分为对照组和电针治疗组,每组15例。对照组不接受任何减肥治疗,电针治疗组采用"接力赛"电针法,主穴选择滑肉门、天枢、外陵、水道、腹结... 详细信息

目的:应用核磁共振成像(MRI)评价"接力赛"电针法对女性腹部脂肪的影响。方法:30例腹型肥胖女性随机分为对照组和电针治疗组,每组15例。对照组不接受任何减肥治疗,电针治疗组采用"接力赛"电针法,主穴选择滑肉门、天枢、外陵、水道、腹结、大横等,每周治疗3次,共治疗3个月。治疗前后对体质量指数(BMI)、腰围(WC)进行测量,MRI扫描计算L4、L5、S3下缘及耻骨联合上缘水平腹壁脂肪厚度及L4-S3所有层面累积脂肪体积并进行疗效评估。结果:治疗后,电针治疗组与对照组比较,BMI,WC,L4、L5、S3下缘及耻骨联合上缘水平腹壁脂肪厚度减少值及L4-S3脂肪体积减少值差异均有统计学意义(P<0.01),电针治疗组优于对照组。电针治疗组耻骨联合上缘水平腹壁脂肪厚度及L4-S3区间脂肪体积治疗后较治疗前明显下降(P<0.01,P<0.05)。结论:"接力赛"电针法可以有效减少腹型肥胖女性腹部脂肪,运用MRI评价腹部脂肪变化更为客观。

关键词：电针腹型肥胖核磁共振成像腹部脂肪

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Laparoscopic liver resection utilizing the ventral avascular area of the inferior vena cava:A retrospective cohort study

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World Journal of Gastroenterology 2025年第1期31卷 42-57页

作者： Kun Huang Zhu chen Heng Xiao Hai-Yang Hu Xing-yu chen cheng-You Du Xiang Lan Department of Hepatobiliary Surgery The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400000China Department of General Surgery Mianyang Hospital of Traditional Chinese MedicineMianyang 621000Sichuan ProvinceChina

BACKGROUND Laparoscopic liver resection(LLR)can be challenging due to the difficulty of establishing a retrohepatic tunnel under *** the third hepatic hilum before parenchymal transection often leads to significant liver mobilization,tumor compression,and bleeding from the short hepatic veins(SHVs).This study introduces a novel technique utilizing the ventral avascular area of the inferior vena cava(IVC),allowing SHVs to be addressed after parenchymal transection,thereby reducing surgical complexity and improving outcomes in in situ *** To introduce and evaluate a novel LLR technique using the ventral avascular area of the IVC and compare its short-term outcomes with conventional *** The clinical cohort data of patients with pathologically confirmed hepatocellular carcinoma or intrahepatic cholangiocarcinoma who underwent conventional LLR and novel LLR between July 2021 and July 2023 at the First Affiliated Hospital of Chongqing Medical University were retrospectively *** novel LLR,we initially separated the caudate lobe from the IVC using dissecting forceps along the ventral avascular area of the ***,we transected the parenchyma of the left and right caudate lobes from the caudal side to the cephalic side using the avascular area as a ***,we addressed the SHVs and finally dissected the root of the right hepatic vein or left hepatic *** short-term postoperative outcomes and oncological results of the two approaches were evaluated and *** A total of 256 patients were included,with 150(58.59%)undergoing conventional LLR and 106(41.41%)undergoing novel *** novel technique resulted in significantly larger tumor resections(6.47±2.96 cm vs 4.01±2.33 cm,P<0.001),shorter operative times(199.57±60.37 minutes vs 262.33±83.90 minutes,P<0.001),less intraoperative blood loss(206.92±37.09 mL vs 363.34±131.27 mL,P<0.001),and greater resection volume(345.11±31.40 mL vs 264.38±31.98 mL,P<0.001)comp

关键词： Laparoscopic liver resection Inferior vena cava Retrohepatic tunnel Short hepatic veins Complex liver tumors Intraoperative bleeding control

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LncRNA编码小肽的功能及研究现状

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中国生物化学与分子生物学报 2021年第12期37卷 1577-1583页

作者：黎秋慧陈瑜丽刘向华南京医科大学基础医学院生物化学与分子生物学系南京211166

长非编码RNA(long non-coding RNAs,lncRNA)是一类长度大于200 nt的RNA,在表观遗传的调控中发挥重要作用。由于缺乏编码性开放阅读框(open reading frame,ORF)、序列保守性低及缺乏已知的蛋白质结构域,lncRNA被认为不能编码蛋白质。随... 详细信息

长非编码RNA(long non-coding RNAs,lncRNA)是一类长度大于200 nt的RNA,在表观遗传的调控中发挥重要作用。由于缺乏编码性开放阅读框(open reading frame,ORF)、序列保守性低及缺乏已知的蛋白质结构域,lncRNA被认为不能编码蛋白质。随着质谱、RNA测序和核糖体分析等先进技术的快速发展和应用,短开放阅读框(short open reading frames,sORFs)被证实具有编码蛋白质的潜力。LncRNA具有sORFs并且能够与核糖体结合。另外,lncRNA类似于mRNA也是由RNA聚合酶II转录而来,因此lncRNA极有可能进行翻译。目前,一些在线工具和实验技术联合运用,成功检测到lncRNA编码的小肽。由于当前实验技术仍存在不足,例如小分子量的肽很难被检测到、小肽的抗体合成困难等,只有较少一部分lncRNA编码小肽被鉴定出来。LncRNA编码的小肽在生物体内发挥重要作用,例如参与胚胎发育、肌细胞发生及肿瘤发生发展等过程。其中,在肿瘤中异常表达的lncRNA编码小肽,有望成为肿瘤治疗的小分子药物、药物靶标和生物标志物,表现出诱人的应用前景。本文对常用的预测和验证lncRNA编码小肽的在线工具和实验技术进行了简单介绍,总结了lncRNA编码小肽的功能并探讨了其在肿瘤中的应用前景,同时也分析了目前研究lncRNA编码小肽所面临的挑战,为更好地理解和研究lncRNA编码小肽提供参考。

关键词：长非编码RNA 短开放阅读框小肽预测与鉴定小肽的功能

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CNKSR2 interactome analysis indicates its association with the centrosome/microtubule system

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Neural Regeneration Research 2025年第8期20卷 2420-2432页

作者： lin Yin Yalan Xu Jie Mu yu Leng Lei Ma yu Zheng Ruizhi li Yin Wang Peifeng li Hai Zhu Dong Wang Jing li Institute for Translational Medicine The Affiliated Hospital of Qingdao UniversityMedical CollegeQingdao UniversityQingdaoShandong ProvinceChina School of Basic Medicine Qingdao UniversityQingdaoShandong ProvinceChina College of life Sciences and School of PharmacyMedical CollegeQingdao UniversityQingdaoShandong ProvinceChina Department of Urology Qingdao Municipal Hospital Affiliated to Qingdao UniversityQingdaoShandong ProvinceChina

The protein connector enhancer of kinase suppressor of Ras 2(CNKSR2),present in both the postsynaptic density and cytoplasm of neurons,is a scaffolding protein with several protein-binding *** of the CNKSR2 gene have been implicated in neurodevelopmental disorders,particularly intellectual disability,although the precise mechanism involved has not yet been fully *** has demonstrated that CNKSR2 plays a role in facilitating the localization of postsynaptic density protein complexes to the membrane,thereby influencing synaptic signaling and the morphogenesis of dendritic ***,the function of CNKSR2 in the cytoplasm remains to be *** this study,we used immunoprecipitation and high-resolution liquid chromatography-mass spectrometry to identify the interactors of *** a combination of bioinformatic analysis and cytological experiments,we found that the CNKSR2 interactors were significantly enriched in the proteome of the *** also showed that CNKSR2 interacted with the microtubule protein DYNC1H1 and with the centrosome marker *** colocalization analysis confirmed the centrosomal localization of *** we downregulated CNKSR2 expression in mouse neuroblastoma cells(Neuro 2A),we observed significant changes in the expression of numerous centrosomal *** manipulation also affected centrosome-related functions,including cell size and shape,cell proliferation,and ***,we found that CNKSR2 interactors were highly enriched in de novo variants associated with intellectual disability and autism spectrum *** findings establish a connection between CNKSR2 and the centrosome,and offer new insights into the underlying mechanisms of neurodevelopmental disorders.

关键词： autism spectrum disorder centrosome CNKSR2 intellectual disability interactome mass spectrometry microtubule neurodevelopmental disease protein complexes protein-protein interactions

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Observation of acoustic hybrid topological phases induced by the p-d orbital interactions

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 132-139页

作者： Xiao Xiang Feng Gao yu-Gui Peng Peng Wu Zong-lin li Xue-Feng Zhu School of Physics and Innovation Institute Huazhong University of Science and Technology

In spinless systems,growing attention has recently been attracted to synthetic gauge fields,which redefine the fundamental crystal symmetries by utilizing the projective algebraic ***,synthetic gauge fields have been commonly investigated in single orbital systems,and explorations on hybrid orbitals remain elusive in ***,we report the experimental observation of hybrid topological phases induced by hybrid synthetic gauge flux,which is formed by the interaction between p and d orbitals embedded in acoustic *** breaking the translation symmetries of Lxand/or Ly,we unambiguously demonstrate the first-order M?bius edge states and higher-order corner *** work establishes a platform for seeking exotic topological phases induced by hybrid-orbitals interactions and initializing the framework of hybrid-orbitalsrelated topological *** applications can be anticipated in scenarios such as high-quality sensing and robust wave trapping due to the stepwise energy distribution of the hybrid topological phase.

关键词： topological insulator synthetic gauge field projective symmetry corner states

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Depression mechanism of sulfite ions on sphalerite and Pb^(2+)activated sphalerite in the flotation separation of galena from sphalerite

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International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 335-345页

作者： Feng Zhang chenyang Zhang linlin Wu Wei Sun Hongliang Zhang Jianhua chen Yong Pei Songjiang li Engineering Research Center of Ministry of Education for Carbon Emission Reduction in Metal Resource Exploitation and Utilization Hunan International Joint Research Center for Efficient and Clean Utilization of Critical Metal Mineral ResourcesSchool of Minerals Processing and BioengineeringCentral South UniversityChangsha 410083China State Key Laboratory of Mineral Processing Beijing General Research Institute of Mining&MetallurgyBeijing 100160China Hunan Provincial Key Laboratory of Micro&Nano Materials Interfaces Science College of Chemistry and Chemical EngineeringCentral South UniversityChangsha 410083China School of Resources Environment and MaterialsGuangxi UniversityNanning 530004China Department of Chemistry Xiangtan UniversityXiangtan 411105China China Railway Resource Group Co.Ltd Beijing 102300China

The depression mechanism of sulfite ions on sphalerite and Pb^(2+)activated sphalerite in the flotation separation of galena from sphalerite still lacked in-depth ***,the depression mechanism of sulfite ions on sphalerite and Pb^(2+)activated sphalerite in the flotation separation of galena from sphalerite was further systematically investigated with experiments and density functional theory(DFT)*** X-ray photoelectric spectroscopy(XPS)results,DFT calculation results,and frontier molecular orbital analysis indicated that sulfite ions were difficult to be adsorbed on sphalerite surface,suggesting that sulfite ions achieved depression effects on sphalerite through other non-adsorption ***,the oxygen content in the surface of sphalerite treated with sulfite ions in creased,which enhanced the hydrophilicity of the sphalerite and further increased the difference in hydrophilicity between sphalerite and ***,sulfite ions were chelated with lead ions to form PbSO_(3)in *** hydrophilic PbSO_(3)was more easily adsorbed on sphalerite than *** interaction between sulfite ions and lead ions could effectively inhibit the activation of *** addition the UV spectrum showed that after adding sulfite ions,the peak of perxanthate in the sphalerite treated xanthate solution was significantly stronger than that in the galena with xanthate solution,indicating that xanthate interacted more readily with sulfite ions and oxygen mo lecules within the sphalerite system,leading to the formation of ***,sulfite ions hardly depressed the flotation of ga lena and could promote the flotation of galena to some *** study deepened the understanding of the depression mechanism o sulfite ions on sphalerite and Pb^(2+)activated sphalerite.

关键词： sphalerite galena sulfite ion density functional theory depression mechanism

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Influence of introducing Zr,Ti,Nb and Ce elements on externally solidified crystals and mechanical properties of high-pressure die-casting Al–Si alloy

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 147-153页

作者： Junjie li Wenbo yu Zhenyu Sun Weichen Zheng liangwei Zhang Yanling Xue Wenning liu Shoumei Xiong Center of Materials Science and Engineering School of Mechanical and Electronic Control EngineeringBeijing Jiaotong UniversityBeijing 100044China Traffic&Transportation Engineering Central South UniversityChangsha 410075China Shanghai Synchrotron Radiation Facility Shanghai Advanced Research InstituteChinese Academy of SciencesShanghai 201204China School of Materials Science and Engineering Tsinghua UniversityBeijing 100084China

High pressure die casting(HPDC)AlSi10Mn Mg alloy castings are widely used in the automobile *** can optimize the mechanical properties of castings through heat treatment,while the release of thermal stress arouses the deformation of large integrated ***,the development of non-heat treatment Al alloys is becoming the hot *** addition,HPDC contains externally solidified crystals(ESCs),which are detrimental to the mechanical properties of *** achieve high strength and toughness of non-heat treatment die-casting Al-Si alloy,we used AlSi9Mn alloy as matrix with the introduction of Zr,Ti,Nb,and *** influences on ESCs and mechanical properties were systematically investigated through three-dimensional reconstruction and thermodynamic *** results reveal that the addition of Ti increased ESCs'size and porosity,while the introduction of Nb refined ESCs and decreased ***,large-sized Al_3(Zr,Ti)phases formed and degraded the mechanical *** introduction of Ce resulted in the poisoning effect and reduced mechanical properties.

关键词： aluminium alloy high-pressure die-casting externally solidified crystals non-heat treatment

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