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Review:Mitochondrial functions on oocytes and preimplantation embryos

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2009年第7期10卷 483-492页

作者： Li-ya WANG Da-hui WANG Xiang-yang ZOU chen-ming xu Department of Biotechnology Dalian Medical University Dalian 116044 China The Sperm Laboratory Institute of Cell Biology and Genetics College of Life Sciences Zhejiang University Hangzhou 310058 China Department of Reproductive Endocrinology Women's Hospital School of Medicine Zhejiang University Hangzhou 310006 China

Oocyte quality has long been considered as a main limiting factor for in vitro fertilization （IVF）. In the past decade, extensive observations demonstrated that the mitochondrion plays a vital role in the oocyte cytoplasm, for it can provide adenosine triphosphate （ATP） for fertilization and preimplantation embryo development and also act as stores of intracellular calcium and proapoptotic factors. During the oocyte maturation, mitochondria are characterized by distinct changes of their distribution pattern from being homogeneous to heterogeneous, which is correlated with the cumulus apoptosis. Oocyte quality decreases with the increasing maternal age. Recent studies have shown that low quality oocytes have some age-related dysfunctions, which include the decrease in mitochondrial membrane potential, increase of mitochondrial DNA （mtDNA） damages, chromosomal aneuploidies, the incidence of apoptosis, and changes in mitochoncLrial gene expression. All these dysfunctions may cause a high level of de- velopmental retardation and arrest of preimplantation embryos. It has been suggested that these mitochondrial changes may arise from excessive reactive oxygen species （ROS） that is closely associated with the oxidative energy production or calcium overload, which may trigger permeability transition pore opening and subsequent apoptosis. Therefore, mitochondria can be seen as signs for oocyte quality evaluation, and it is possible that the oocyte quality can be improved by enhancing the physical function of mitochondria. Here we reviewed recent advances in mitochondrial functions on oocytes.

关键词： Mitochondria Oocyte Preimplantation embryo

Effect of preparation methods on Pt/alumina catalysts for the hydrogen iodide catalytic decomposition

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Chinese Chemical Letters 2009年第1期20卷 102-105页

作者： Zhi Chao Wang Lai Jun Wang Ping Zhang Song Zhe chen Jing ming xu Jing chen Laboratory 201 Institute of Nuclear and New Energy Technology Tsinghua University Beijing 102201 China

Three kinds of Pt/alumina catalysts were prepared by impregnation-hydrogen reduction, impregnation-hydrazine reduction and electroless plating methods. Their differences in the structures, specific areas and particle sizes were characterized by XRD, BET and TEM, respectively. Furthermore, their catalytic activities for the hydrogen iodide （HI） decomposition were evaluated in a fixed bed reactor. The results show that the catalyst 5%Pt/Al2O3 prepared by the electroless plating has the optimum catalytic properties for HI decomposition owing to the high dispersion of the platinum nano-particles （〈5 nm） on the alumina supports.

关键词： Hydrogen iodide Catalytic decomposition Pt/At2O3 Preparation methods

Accuracy of a combined score of zygote and embryo morphology for selecting the best embryos for IVF

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2008年第8期9卷 649-655页

作者： Yu-li QIAN Ying-hui YE chen-ming xu Fan JIN He-feng HUANG Department of Reproductive Endocrinology Women＇s Hospital School of Medicine Zhejiang University Hangzhou 310006 China

Objective:To evaluate the accuracy of a scoring system combining zygote and embryo morphology in predicting the outcome of in vitro fertilization (IVF) treatment. Methods: In a study group, 117 consecutive IVF or intracytoplasmic sperm injection (ICSI) cycles with embryo transfer were carried out and 312 embryos were scored using a combined scoring system (CSS) of zygote and embryo morphology before transplantation. In a control group, a total of 420 IVF or ICSI cycles were carried out and 1176 embryos were scored using a cumulative embryo score (CES). The effects of the combined scoring system on the embryo implantation rate and pregnancy rate per cycle were analyzed. Results: Using the combined scoring system, the embryo implan-tation rate (27.6%) and the clinical pregnancy rate (48.7%) were significantly higher than those in the control group (20.8% and 38.6%, respectively). Also, the implantation rate of embryos scoring ≥70 (38.5%: 82 sacs/213 embryos) was significantly higher (P<0.001) than that of embryos scoring <70 (4%: 4 sacs/99 embryos). The pregnancy rate of patients with embryos scoring ≥70 using the combined scoring system (66.7%) was significantly higher (P<0.001) than that of patients with embryos scoring ≥20 using the cumulative embryo score (59.0%). Conclusion: The results suggest that selecting embryos with a high score (≥70) using the combined scoring system could increase the implantation rate and pregnancy rate, and that using a scoring system combining assessments of human zygotes and pre-implantation embryos might predict IVF outcomes more accurately than using a cumula-tive embryo score.

关键词： Embryo score Combined scoring system (CSS) Implantation In vitro fertilization (IVF) Pregnancy

On Generality of the Data Plane and Scalability of the Control Plane in Software-Defined Networking

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China Communications 2014年第2期11卷 55-64页

作者： ZUO Qingyun chen ming DING Ke xu Bo College of Command Information Systems PLA University of Science and Technology Nanjing 210007 China

The control and data planes are decoupled in software-defined networking(SDN),which enables both planes to evolve independently,and brings about many advantages such as high flexibility,programmability,and rapid implementation of new network ***,in order to improve the scalability of the control plane at present,some control functionalities are added to the data plane,which is probably to impact on the generality of the data *** key challenge of adding control functionalities to the data plane is to strike a careful balance between the generality of the data plane and the scalability of the control *** propose some basic principles that both control and data planes should comply with,based on the evolutionary trend of ***,we take two approaches for reference according to the principles,viewed from the control messages in OpenFlow-based *** evaluations demonstrate that the approaches can maintain the generality of the data plane and improve the scalability of the control plane.

关键词： OpenFlow SDN architecture control functionalities control message

Chemokine stromal cell-derived factor 1/CXCL12 increases homing of mesenchymal stem cells to injured myocardium and neovascularization following myocardial infarction

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Chinese Medical Journal 2009年第2期122卷 183-187页

作者： ZHUANG Yu chen Xin xu ming ZHANG Lei-yang XIANG Fei Department of Thoracic and Cardiovascular Surgery Nanjing First Hospital Affiliated to Nanjing Medical University Nanjing Jiangsu 210006 China

Background Heart failure due to ischemic heart disease is still a major health problem. Myocardium regeneration emerges as a novel therapeutic method for treating myocardial infarction （MI）. However, it is affected by many factors. The present study was aimed to investigate the effect of chemokine stromal cell-derived factor 1 （SDF-1）/CXCL12 on mesenchymal stem cells （MSCs） homing to injured myocardium in a rat myocardial infarction model. Methods A rat myocardial infarction model was established by permanent left anterior descending branch ligation. Mesenchymal stem cells from donor rats were cultured in IMDM and labeled with bromodeoxyuridine. The rats were divided into two groups. SDF-1 expression was measured by in situ hybridization and immunohistochemistry in the sham operated or infarcted hearts at 1, 2, 4, 7, 14 and 28 days post operation in the SDF-1 detection group. The rats in the intervention groups were injected with SDF-1, anti-SDF-1 antibody or saline 4 days after myocardial infarction. Then, a total of 5×10^6 cells in 2.5 ml of phosphate-buffered saline were injected through the tail vein. The number of the labeled MSCs in the infarcted hearts was counted on the 3rd day post injection. Cardiac function and blood vessel density were assessed on the 28th day post injection. Results Self-generating SDF-1 expression was increased at the first day post MI, peaked at the 7th day and decreased thereafter while it remained unchanged in sham operated hearts. The MSCs enrichment in the host hearts were more abundant in the MI groups than in the non-MI group （P=0.000）; the MSCs enrichment in the host hearts were more abundant in the SDF-1 injected group than in the anti-SDF-1 antibody and saline injected groups （P = 0.000）. Cardiac function was improved more in the SDF-1 injected group than in the anti-SDF-1 antibody and saline injected groups （P = 0.000）. Neovascularization in the SDF-1 injected group increased significantly compared to the other groups （P= 0.

关键词： mesenchymal stem cells homing behavior myocardial infarction chemokine CXCL12

First clinical experience of preimplantation genetic diagnosis(PGD) for XLP

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First clinical experience of preimplantation genetic diagnos...

Effects of ＂Phone Consultation＂ to Mental Status and Quality of Life of Patients with Cancer-related Pain and Physicians＇ Work and Life

2013浙江省医学遗传学学术年会

作者： Song-Chang chen chen-ming xu Key Laboratory of Reproductive Genetics National Ministry of Education(Zhejiang University)Women’s Reproductive Healthy Laboratory of Zhejiang Province

X-linked lymphoproliferative(XLP) is a rare inherited immunodeficiency disease that is characterized by severe immune dysregulation following Epstein-Barr virus *** 60 percent of cases of XLP are caused by mutations in SH2D1A that encodes for signaling lymphocytic activation molecule(SLAM)-associated protein(SAP).Pre-implantation genetic diagnosis(PGD ) refers to genetic profiling of embryos prior to implantation and is considered an alternative form of prenatal *** combining in vitro fertilization (IVF) with single-cell genetic analysis at the preimplantation stages,PGD offers a way to test patient’s embryos for genetic disorders and only allows unaffected embryos to be transferred to the *** has been applied worldwide for a great variety of single-gene disorders over the last 20 years. The aim of this work was to perform PGD for a couple at risk of X-linked lymphoproliferative carrying SH2D1A gene mutation(c.192G>A).Whole genome amplification(WGA) followed by PCR and directly sequencing was used to identify SH2D1A mutation for PGD.10 oocytes were fertilized,5 embryos were suitable for embryo biopsy,2 were diagnosed as unaffected,1 were carriers,2 were *** unaffected embryo were transfered and an unaffected embryo was implanted,leading to a successful *** our knowledge,this is the first report of clinical experience of preimplantation genetic diagnosis(PGD) for XLP.

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麻醉与监护论坛 2011年第2期18卷 119-121页

作者： Zhi-yang chen Jian-ming xu Yue-ling chen Pain Clinic Anesthesia Department of Cancer Hospital Department of Oncology Fudan University Shanghai P.R.China 200032

Application and Challenge of Preimplantation Genetic Testing in Reproductive Medicine

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Reproductive and Developmental Medicine 2019年第3期3卷 129-132页

作者： xue-Li Liu chen-ming xu He-Feng Huang Department of Reproductive Medicine Institute of Embryo-Fetal Original Adult DiseaseSchool of MedicineInternational Peace Maternity and Child Health HospitalShanghai Jiao Tong UniversityShanghai 200030China

There has been a very rapid development in the area of preimplantation genetic testing(PGT)since the first baby applied PGT technology in the world was born in the last 30 *** an alternative treatment of prenatal diagnosis,the transfer of the fetus with normal testing result has benefited many *** is mainly classified into PGT for aneuploidies(PGT-A),PGT for chromosomal structural rearrangement(PGT-SR),and PGT for monogenic/single-gene defects(PGT-M).Here,we reviewed the application,development,limitations,and challenges of the current PGT in reproductive medicine.

关键词： diagnosis fetus implantation

过渡金属催化的有机硼试剂对亚胺的不对称加成研究进展

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有机化学 2017年第7期37卷 1589-1612页

作者：陈雕徐明华中国科学院上海药物研究所新药研究国家重点实验室上海201203

手性胺是有机合成中一类非常重要的合成砌块,在天然产物、生物活性分子及药物分子中广泛存在,因此发展高效的构建手性胺类化合物的方法,一直都是合成有机化学家们关注的重点.有机硼试剂近年来以其低毒、稳定、易得等优点逐渐受到合成化... 详细信息

手性胺是有机合成中一类非常重要的合成砌块,在天然产物、生物活性分子及药物分子中广泛存在,因此发展高效的构建手性胺类化合物的方法,一直都是合成有机化学家们关注的重点.有机硼试剂近年来以其低毒、稳定、易得等优点逐渐受到合成化学家的青睐,被越来越多地用于过渡金属催化的反应中.本文综述了近十多年来基于手性辅剂及手性催化策略的过渡金属催化的有机硼试剂对亚胺的不对称加成研究进展,着重探讨了各种手性配体在反应中的应用以及α-手性胺类化合物的立体选择性构建.

关键词：不对称合成手性胺金属催化不对称加成有机硼试剂