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Single-cell RNA-sequencing combined with bulk RNA-sequencing analysis of peripheral blood reveals the characteristics and key immune cell genes of ulcerative colitis

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World Journal of Clinical Cases 2022年第33期10卷 12116-12135页

作者： Yan-cheng Dai Dan Qiao chen-ye fang Qiu-Qin chen Ren-ye Que Tie-Gang Xiao Lie Zheng Li-Juan Wang Ya-Li Zhang Department of Gastroenterology Shanghai Traditional Chinese Medicine-Integrated HospitalShanghai University of Traditional Chinese MedicineShanghai 200082China Department of Gastroenterology Traditional Chinese Medicine Hospital of NingboNingbo 315000Zhejiang ProvinceChina Department of Pathology Shanghai Traditional Chinese Medicine-Integrated HospitalShanghai University of Traditional Chinese MedicineShanghai 200082China Department of Gastroenterology Traditional Chinese Medicine Hospital of Xi’an 730000Shaanxi ProvinceChina Experimental Education Center Shanghai University of Traditional Chinese MedicineShanghai 201203China Institute of Digestive Diseases LongHua Hospital Shanghai University of Traditional Chinese MedicineShanghai 200032China

BACKGROUND Ulcerative colitis(UC)is a complicated disease caused by the interaction between genetic and environmental factors that affects mucosal homeostasis and triggers an inappropriate immune ***-cell RNA sequencing(scRNA-seq)can be used to rapidly obtain the precise gene expression patterns of thousands of cells in the intestine,analyze the characteristics of cells with the same phenotype,and provide new insights into the growth and development of intestinal organs,the clonal evolution of cells,and immune cell *** findings can provide new ideas for the diagnosis and treatment of intestinal *** identify clinical phenotypes and biomarkers that can predict the response of UC patients to specific therapeutic drugs and thus aid the diagnosis and treatment of *** Using the Gene Expression Omnibus(GEO)database,we analyzed peripheral blood cell subtypes of patients with UC by scRNA-seq combined with bulk RNA sequencing(RNA-seq)to reveal the core genes of *** then combined weighted gene correlation network analysis(WGCNA)and least absolute shrinkage and selection operator(LASSO)analysis to reveal diagnostic markers of *** After processing the scRNA-seq data,we obtained data from approximately 24340 cells and identified 17 cell *** intercellular communication analysis,we selected monocyte marker genes as the candidate gene set for the prediction *** of a WGCNA coexpression network identified RhoB,cathepsin D(CTSD)and zyxin(ZYX)as core *** infiltration analysis showed that these three core genes were strongly correlated with immune *** enrichment analysis showed that the differentially expressed genes were closely related to immune and inflammatory responses,which are associated with many challenges in the diagnosis and treatment of *** Through scRNA-seq analysis,LASSO diagnostic model building and WGCNA,we identified RhoB,CTSD and ZYX as core genes of UC that are closel

关键词： Ulcerative colitis Single-cell RNA-seq Bulk RNA-seq Peripheral blood Key genes

A Blockchain-Based Proxy Re-Encryption Scheme with Conditional Privacy Protection and Auditability

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China Communications 2024年第7期21卷 267-277页

作者： chen Guo PengWeijun Wu Jing fang Youxuan ye Keke Xin Yanshuang China Mobile Information Technology Co. LtdBeijing 100037China China Mobile Information Technology Co. LtdShenzhen 518048China

With the development of Internet of Things technology,intelligent door lock devices are widely used in the field of house *** the traditional housing leasing scenario,problems of door lock information disclosure,tenant privacy disclosure and rental contract disputes frequently occur,and the security,fairness and auditability of the housing leasing transaction cannot be *** solve the above problems,a blockchain-based proxy re-encryption scheme with conditional privacy protection and auditability is *** scheme implements fine-grained access control of door lock data based on attribute encryption technology with policy hiding,and uses proxy re-encryption technology to achieve auditable supervision of door lock information *** encryption technology and zero-knowledge proof technology are introduced to ensure the confidentiality of housing rent information and the fairness of rent *** construct a decentralized housing lease transaction architecture,the scheme realizes the efficient collaboration between the door lock data ciphertext stored under the chain and the key information ciphertext on the chain based on the blockchain and InterPlanetary File ***,the security proof and computing performance analysis of the proposed scheme are carried *** results show that the scheme can resist the chosen plaintext attack and has low computational cost.

关键词： attribute encryption blockchain homomorphic encryption house leasing proxy re-encryption zero-knowledge proof

Pairs of thiol-substituted 1,2,4-triazole-based isomeric covalent inhibitors with tunable reactivity and selectivity

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Chinese Chemical Letters 2024年第7期35卷 329-335页

作者： Shiqi Xu Zi ye Shuang Shang Fengge Wang Huan Zhang Lianguo chen Hao Lin chen chen fang Hua Chong-Jing Zhang State Key Laboratory of Bioactive Substance and Function of Natural Medicines Beijing Key Laboratory of Active Substance Discovery and Druggability EvaluationInstitute of Materia MedicaChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100050China State Key Laboratory of Bioactive Substance and Function of Natural Medicines Beijing Key Laboratory of New Drug Mechanisms and Pharmacological Evaluation Study(BZ0150)Institute of Materia MedicaPeking Union Medical College and Chinese Academy of Medical SciencesBeijing 100050China The First Affiliated Hospital of Wenzhou Medical University Wenzhou 325000China

Covalent bioactive compounds are successfully used in clinic and attracted intense research efforts in the fundamental study as well as drug *** advantageous effects of covalent compounds compared with non-covalent ones are highly dependent on electrophilic ***,electrophilic warheads with tunable reactivity and selectivity are highly demanded in fields of medicinal chemistry and chemical ***,we report a novel electrophilic warhead,chloromethyl group activated by thiol-substituted 1,2,***,a pair of regioisomers could be simultaneously occurred in the step of alkylation during the synthesis of this unique *** is a rare example that the alkylation could simultaneously generate these two separable regioisomers of 1,2,4-triazole at the nitrogen or sulfur *** covalent-working mechanism of this new warhead is confirmed by various chemoproteomics experiments including target identification and binding site ***,the reactivity and selectivity of this new electrophilic warhead could be efficiently tuned by virtue of stereo ***,one pair of regioisomers(19S and 19X)induced distinct modes of cell *** 19S could induce apoptosis of colon cancer cells while 19X could induce both apoptosis and ***,this study provides pairs of novel electrophilic warheads that could be useful not only in supporting the design of covalent compounds for drug discovery but also in providing chemical probes for the fundamental biological study.

关键词： Electrophilic warhead Covalent inhibitor 1,2,4-Triazole Chemical proteomics GSTO1

Advancing cell-based therapy in sepsis:An anesthesia outlook

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Chinese Medical Journal 2024年第13期137卷 1522-1534页

作者： Hui ye Xiaoyu Zou Xiangming fang Department of Anesthesiology and Intensive Care The First Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhouZhejiang 310003China The Children’s Hospital National Clinical Research Center for Child HealthSchool of MedicineZhejiang UniversityHangzhouZhejiang 312000China

Sepsis poses a health challenge globally owing to markedly high rates of morbidity and *** employing bundle therapy over two decades,approaches including transient organ supportive therapy and clinical trials focusing on signaling pathways have failed in effectively reversing multiple organ failure in patients with *** and appropriate perioperative management for surgical patients with concurrent sepsis is ***,innovative therapies focusing on remedying organ injuries are *** therapy has emerged as a promising therapeutic avenue for repairing local damage to vital organs and restoring homeostasis during perioperative treatment for *** the pivotal role of immune cell responses in the pathogenesis of sepsis,stem cell-based interventions that primarily modulate immune responses by interacting with multiple immune cells have progressed into clinical *** strides made in single-cell sequencing and gene-editing technologies have advanced the understanding of disease-specific immune responses in *** antigen receptor(CAR)-immune cell therapy offers an intriguing option for the treatment of *** review provides a concise overview of immune cell therapy,its current status,and the strides made in the context of sepsis research,discussing potential strategies for the management of patients with sepsis during perioperative stages.

关键词： Sepsis Perioperative Receptors,chimeric antigen Immunotherapy,adoptive Perfusion Tissue repair Multiple organ failure Shock,septic

Data-driven discovery of linear dynamical systems from noisy data

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Science China(Technological Sciences) 2024年第1期67卷 121-129页

作者： WANG YaSen YUAN ye fang HuaZhen DING Han School of Mechanical Science and Engineering Huazhong University of Science and TechnologyWuhan 430074China State Key Lab of Digital Manufacturing Equipment and Technology Huazhong University of Science and TechnologyWuhan 430074China School of Artificial Intelligence and Automation Huazhong University of Science and TechnologyWuhan 430074China Department of Mechanical Engineering University of KansasLawrence 66045USA

In modern science and engineering disciplines,data-driven discovery methods play a fundamental role in system modeling,as data serve as the external representations of the intrinsic mechanisms within ***,empirical data contaminated by process and measurement noise remain a significant obstacle for this type of *** this study,we have developed a data-driven method capable of directly uncovering linear dynamical systems from noisy *** method combines the Kalman smoothing and sparse Bayesian learning to decouple process and measurement noise under the expectation-maximization framework,presenting an analytical method for alternate state estimation and system ***,the discovered model explicitly characterizes the probability distribution of process and measurement noise,as they are essential for filtering,smoothing,and stochastic *** have successfully applied the proposed algorithm to several simulation *** results demonstrate its potential to enable linear dynamical system discovery in practical applications where noise-free data are intractable to capture.

关键词： system identification sparse Bayesian learning Kalman smoothing process and measurement noise

Effect of yttrium and manganese addition on catalytic soot combustion activity and anti-high-temperature stability of CeO_(2) catalyst

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Journal of Rare Earths 2024年第2期42卷 334-344,I0004页

作者： Yi Zhu Xingyu Liu Wei Shi Jia Li Changbing ye Ruimei fang Huifeng Zhu Shanhu chen Li Lan College of Chemistry Biology and Environment Yuxi Normal UniversityYuxi 653100China Institute of Biology and Environmental Engineering Yuxi Normal UniversityYuxi 653100China College of Environment and Resources Chongqing Technology and Business UniversityChongqing 400067China College of Chemistry and Chemical Engineering Jiangxi Science and Technology Normal UniversityNanchang 330013China College of Materials and Mechatronics Jiangxi Science and Technology Normal UniversityNanchang 330013China

In order to analyze the influence of the addition of yttrium and manganese on the soot combustion performance and high temperature stability of CeO_(2) catalyst,a series of Y/Mn-modified CeO_(2) catalysts were *** effects of structural properties,textural properties,oxygen vacancies,Ce^(3+),surface adsorbed oxygen species,reduction properties and desorption properties of oxygen species on the activity were analyzed by various characterization *** results of the activity test show that the addition of manganese is beneficial to enhancement of the activity,while the addition of yttrium increases the amount of reactive oxygen species,but decreases the *** aging at 700℃,the activity of the CeMn catalyst decreases most sharply,while the catalytic activity of the CeY catalyst can be maintained to a certain ***,the addition of yttrium and manganese at the same time can stabilize the *** fundamental reason is that yttrium and manganese move to the surface of the solid solution after aging,which increases the reduction performance of the catalyst,thus contributing to the increase of *** the activity of CeYMn catalyst decreases after aging at 800℃,it is still higher than that of other catalysts aged at 700℃.

关键词： CeO_(2)catalyst Soot catalytic combustion Diesel pollution control Rare earths High temperature stability Low temperature reduction performance

Liquid-metals-induced formation of MXene/polyacrylamide composite organohydrogels for wearable flexible electronics

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Nano Research 2024年第3期17卷 1913-1922页

作者： Minying Wu Bin chen Xueliang Fan Tong ye Yuanyuan fang Qian Zhang fangzhou Zhou Yajun Wang Yi Tang Department of Chemistry Fudan UniversityShanghai 200433China College of Chemistry&Materials Engineering Wenzhou UniversityWenzhou 325027China

Organohydrogels have demonstrated superior environmental adaptability and frost resistance compared to conventional hydrogels,thereby prompting considerable interests in the development and design of innovative ***,we report an effective one-pot method for fabricating MXene/polyacrylamide(MXene/PAM)composite organohydrogel(MAOH)by employing Ga liquid metals(Ga LMs)as a highly reactive component in the induced free radical polymerization reaction,without the need for additional heating or cross-linking *** synthetic protocol addresses the time-consuming and organic solvent waste concerns associated with traditional solvent displacement methods for organohydrogel *** incorporation of MXene not only highly enhances the conductivity but also confers improved mechanical properties of *** MAOH exhibits excellent environmental adaptability(>7 d),sustained moisture retention,remarkable self-healing capabilities,and outstanding mechanical properties under low temperatures(-20℃).It demonstrates exceptional performance in micro-motion monitoring,rapid response time(125 ms),superior stretchability,and a broad range of strains(0.3%–600%).Therefore,the designed MAOH has great potential for applications in diverse fields such as prosthetics,electronic skin,human–machine interaction,and smart terminals.

关键词： organohydrogels MXene conductivity mechanical properties environmental adaptability

Imrecoxib: a novel and selective cyclooxygenase 2 inhibitor with anti-inflammatory effect

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Acta Pharmacologica Sinica 2004年第7期25卷 81-85页

作者： Xiao-hong chen, Jin-ye BAI, fang SHEN, Ai-ping BAI, Zong-ru GUO, Gui-fang chenG2 Departmentof Pharmacology, Institute of Materia Medica, Chinese Academyof Medical Sciencesand Peking Union Medical College, Beijing 100050, China Department of Pharmacology Institute of Materia Medica Chinese Academy of Medical Sciences and Peking Union Medical College 北京 100050 Department of Pharmacology Institute of Materia Medica Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100050 Department of Pharmacology Institute of Materia Medica Chinese Academy of Medical Sciences and Peking Union Medical College

AIM: To investigate the inhibitory effect of imrecoxib, a synthetic compound of completely new structure, on cyclooxygenase 1 (COX-1) and 2 (COX-2) and its anti-inflammatory effect in vivo. METHODS: The inhibitory effects of imrecoxib on cyclooxygenase 1 and 2 were studied using whole cell assay with murine peritoneal mac- rophages induced by calcimycin and LPS. The inhibitory effects of imrecoxib on mRNA level of COX-1 and COX- 2 in human macrophage cell line U937 were detected by reverse transcription polymerase chain reaction (RT-PCR) analysis. Effects of imrecoxib on acute and chronic inflammation were evaluated in rat carrageenan induced edema model and rat adjuvant-induced arthritis model, respectively. RESULTS: Imrecoxib was found to inhibit COX-1 and COX-2 with IC50 value of 115±28 nmol/L and 18±4 nmol/L, respectively. Imrecoxib was shown to selectively and dose-dependently inhibit COX-2 mRNA level. Imrecoxib effectively inhibited carrageenan-induced acute in- flammation at the doses of 5, 10, and 20 mg·kg-1 ig and adjuvant-induced chronic inflammation at the doses of 10 and 20 mg·kg-1·d-1 ig. CONCLUSION: Imrecoxib is a novel and moderately selective COX-2 inhibitor that pos- sesses anti-inflammatory effect by inhibition of COX-2 mRNA expression.

关键词： imrecoxib cyclooxygenase 1 cyclooxygenase 2 cyclooxygenase inhibitors inflammation

Spatially explicit reconstruction of cropland cover in Europe from AD 1800 to 2000

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Science China Earth Sciences 2024年第9期67卷 2953-2971页

作者： Jun LI Yu ye Xiuqi fang Diyang ZHANG Zhilei WU Faculty of Geographical Science Beijing Normal UniversityBeijing 100875China Key Laboratory of Environmental Change and Natural Disaster Ministry of EducationBeijing Normal UniversityBeijing 100875China College of Geographical Science Qinghai Normal UniversityXining 810016China

One of the challenges in global change research is the significant uncertainty in global historical land use and land cover(LUCC)datasets,which are widely used as foundational *** addition to the regional cropland area reconstructions,improving the grid allocation method is another feasible way to raise the reliability of historical LUCC *** this study,an integrated reconstruction of the national cropland areas over the past 200 years was developed for 36 European *** that,the allocation algorithm was built using physiogeographic variables and historical city sites for accounting for land suitability and cultivation preferences,***,cropland data in Europe with a spatial resolution of 5′×5′at five time sections from AD 1800 to 2000 were generated using the optimal allocation algorithm in accordance with the stages of the regional *** results were as follows:(1)The dominant factors governing the distribution of croplands in Europe vary at different agricultural stages,but the results can be merged *** suitability was more optimal for allocation during the modern agricultural stage(AD 1950 and 2000);the priority index combined with land suitability and cultivation preference was more reasonable for allocation during the traditional agricultural stage(AD 1800).The average of the allocations by priority index and the land suitability could be adopted as the allocation results during the transitional stage(AD 1850 and 1900)because the grids for absolute differences within±10 and±20 percentage points between the results obtained from the above two allocations were above 80% and 95%,respectively,which means the two allocation results could be merged.(2)Over the past 200 years,the total cropland area in Europe first increased to a peak in AD 1900 and then ***,the centre of the higher cropland fraction shifted from the western part of Europe in AD 1800 to the eastern part of the continent after AD 1950.(3)

关键词： Land use and land cover change Historical cropland Gridding allocation Europe Past 200 years