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Association mapping of quantitative trait loci for yield-related agronomic traits in rice(Oryza sativa L.)

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Journal of Integrative Agriculture 2016年第10期15卷 2192-2202页

作者： XU Fei-fei JIN Liang HUANG yan TONG Chuan chen ya-ling BAO Jin-song Institute of Nuclear Agricultural Sciences Zhejiang University Research & Development Centre of Flower Zhejiang Academy of Agricultural Sciences

High yield in rice mainly depends on large grain weight, ideal plant architecture and proper flowering time adapting to various geographic regions. To help achieve higher yield, phenotype variations of heading date（HD）, plant architecture and grain shape in a panel of 416 rice accessions were investigated in this study. A total of 143 markers including 100 simple sequence repeat（SSR） markers and 43 gene-tagged markers were employed in association mapping to detect quantitative trait loci（QTL） responsible for these variations. Among the 7 subpopulations, POP5 in japonica group showed the largest values of HD and grain width（GW）, but the smallest values of grain length（GL） and grain length to width ratio（GLW）. Among the six indica groups, POP7 had the largest values of HD, GL, GLW, and 1 000-grain weight（TGW）. A total of 27 QTLs were detected underlying these phenotypic variations in single year, while 12 of them could be detected in 2006 and 2007. GS3 marker was closely associated with GL, GW and GLW, and widely distributed in different groups. The starch synthesis related gene markers, SSI, SSIIa, SBE1, AGPL4, and ISA1, were linked to plant height（PH）, panicle length（PL）, flag leaf length（FLL）, GW, and GLW. The SSR markers, RM267, RM340 and RM346, were linked to at least two traits. Therefore, these new markers will probably be used to improve rice grain yield or plant architecture when performing marker-assisted selection of proper alleles.

关键词： yield plant architecture heading date association mapping marker-assisted selection

Rapid mapping of candidate genes for cold tolerance in Oryza rufipogon Griff. by QTL-seq of seedlings

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Journal of Integrative Agriculture 2018年第2期17卷 265-275页

作者： LUO Xiang-dong LIU Jian ZHAO Jun DAI Liang-fang chen ya-ling ZHANG ling ZHANG Fan-tao HU Biao-lin XIE Jian-kun College of Life Science Jiangxi Normal University Rice Research Institure Jiangxi Academy of Agricultural Sciences

Cold stress is a major problem in rice production. To rapidly identify genes for cold tolerance in Dongxiang wild rice（DWR, Oryza rufipogon Griff.）, sequencing-based bulked segregant analysis of QTL-seq method was used to resequence the extremely resistant（R） and susceptible（S） bulks of a backcross inbred lines（BILs） population（derived from Oryza sativa×O. rufipogon） and their parents. Single nucleotide polymorphisms（SNP）-index graphs and corresponding Δ（SNPindex） graphs（at 99 and 95% confidence levels） for R-and S-bulks detected a total of 2 609 candidate SNPs, including 58 candidate cold-tolerance genes. Quantitative real-time PCR analysis revealed that 5 out of the 58 candidate genes had significant differences in expression between O. sativa and O. rufipogon. Structural variation and functional annotations of the 5 candidate genes were also analyzed, and allowed us to identify 2 insertion-deletion（InDel） markers（12-7 and 12-16） that were linked with candidate genes on chromosome 12 in DWR. These results are helpful for cloning and using cold tolerance genes from common wild rice in cultivated rice.

关键词： common wild rice cold tolerance QTL-seq single nucleotide polymorphisms(SNP)

Genetic Diversity and Genetic Changes in the Introgression Lines Derived from Oryza sativa L. Mating with O. rufipogon Griff.

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Journal of Integrative Agriculture 2012年第7期11卷 1059-1066页

作者： DENG Xiao-juan LUO Xiang-dong DAI Liang-fang chen ya-ling HU Biao-lin XIE Jian-kun College of Life Science Jiangxi Normal University Nanchang 330022 P.R.China Rice Research Institute Jiangxi Academy of Agricultural Sciences Nanchang 330200 P.R.China

The objectives of the present study were to estimate genetic diversity and genetic changes of introgression lines （ILs） which derived from cultivated rice （Oryza sativa L. cv. Xieqingzao B, XB） mating with common wild rice （O. rufipogon Griff., CWR）. The genetic data of 239 ILs were based on a total of 131 polymorphic microsatellite （SSR） markers distributed across the 12 chromosomes of rice. On average, these ILs possessed 77.1 and 14.31% homozygous bands from XB and CWR, respectively. Most of the ILs were clustered together with XB individual, which was revealed by principal coordinate analysis （PCA） and the program STRUCTURE analysis. The result from PCA demonstrated that some intermediate genotypes between XB and CWR were also found. Moreover, there were some genomic sequence changes including parental bands elimination and novel bands emergence in the ILs. The average Nei＇s gene diversity （He） was 0.296, which was higher than that of cultivated rice. It suggested that interspecific hybridization and gene introgression could broaden the base of genetic variation and lay an important foundation for rice genetic improvement. These different genotypic ILs would provide a better experimental system for understanding the evolution of rice species and the mechanism of alien gene introgression.

关键词： Oryza rufipogon Grill. alien gene introgression genetic diversity genetic variation

Genotype × Environment Interactions for Agronomic Traits of Rice Revealed by Association Mapping

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Rice science 2014年第3期21卷 133-141页

作者： XU Fei-fei TANG Fu-fu SHAO ya-fang chen ya-ling TONG Chuan BAO Jin-song Institute of Nuclear Agricultural Sciences College of Agriculture and BiotechnologyHuajiachi CampusZhejiang University

Agronomic traits are important determinants to rice yield, which are controlled by complex genetic factors as well as genotype by environment （G × E） interaction effects. The G × E effects for agronomic traits of rice have been dissected with various approaches, but not with the current available approach, the association studies. In this study, a total of 32 655 single nucleotide polymorphisms were used to carry out associations with 14 agronomic traits among 20 rice accessions in two environments. The G × E interaction effects for all the agronomic traits were at highly significant levels （P〈0.01）, accounting for 3.4%-22.3% of the total sum of squares except for the length of brown rice. Twenty three putative quantitative trait loci （QTLs）, including five previously known and several new promising associations, were identified for 10 of 14 traits. Analysis of the relationships between the traits for which QTLs and the genotype effects could be identified suggested that the higher the genotypic effect, the higher the possibility to identify QTLs for the given trait. The new QTLs detected in this study will facilitate dissection of the complex agronomic traits and may give insight into the G × E effects with association mapping.

关键词： rice yield agronomic trait association mapping single nucleotide polymorphism

THE　MULTI─ASSOCIATION　MODEL　OF　THE　ESR─ACIDITY　SENSITIVE　FREE　RADICAL：A　THEORETICAL　STUDY

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中国科学院大学学报(中英文) 1995年第1期 52-60页

作者： Zhu Jina yan Jing chen ya-ling Zhang Jian-zhong yan Da-yu

ＴＨＥＭＵＬＴＩ─ＡＳＳＯＣＩＡＴＩＯＮＭＯＤＥＬＯＦＴＨＥＥＳＲ─ＡＣＩＤＩＴＹＳＥＮＳＩＴＩＶＥＦＲＥＥＲＡＤＩＣＡＬ：ＡＴＨＥＯＲＥＴＩＣＡＬＳＴＵＤＹ￥ＺｈｕＪｉｎａ；ＹａｎＪｉｎｇ；ＣｈｅｎＹａ－ｌｉｎｇ；... 详细信息

ＴＨＥＭＵＬＴＩ─ＡＳＳＯＣＩＡＴＩＯＮＭＯＤＥＬＯＦＴＨＥＥＳＲ─ＡＣＩＤＩＴＹＳＥＮＳＩＴＩＶＥＦＲＥＥＲＡＤＩＣＡＬ：ＡＴＨＥＯＲＥＴＩＣＡＬＳＴＵＤＹ￥ＺｈｕＪｉｎａ；ＹａｎＪｉｎｇ；ＣｈｅｎＹａ－ｌｉｎｇ；ＺｈａｎｇＪｉａｎ－ｚｈｏｎｇ；Ｙ...

关键词： multi-association model ESR-acidity free radical

求解分布式两阶段装配流水车间调度的帝国竞争-协作算法

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控制理论与应用 2021年第12期38卷 1957-1967页

作者：陈雅玲雷德明武汉理工大学自动化学院湖北武汉430070

针对分布式两阶段装配流水车间调度问题(DTAFSP),提出了一种新型优化方法-帝国竞争-协作算法(ICCA)以最小化总延迟时间.该算法在帝国竞争算法基础上,结合历史进化数据,提出一种基于最强帝国搜索能力与最弱帝国计算资源之间自适应交换的... 详细信息

针对分布式两阶段装配流水车间调度问题(DTAFSP),提出了一种新型优化方法-帝国竞争-协作算法(ICCA)以最小化总延迟时间.该算法在帝国竞争算法基础上,结合历史进化数据,提出一种基于最强帝国搜索能力与最弱帝国计算资源之间自适应交换的帝国间协作方法,并给出一种新型帝国竞争策略,以提高算法搜索效率.进行了大量计算实验,计算结果验证了ICCA策略的合理性和有效性以及它在求解DTAFSP方面较强的搜索优势.

关键词：帝国竞争算法分布式调度帝国协作装配

古典教师角色的现代性异化及复归——基于汉娜·阿伦特的哲学视角

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教师教育研究 2019年第3期31卷 6-12页

作者：陈亚凌华中师范大学教育学院

教师角色是教师在教育领域开展教学的形象预设,它承载着社会对教师的期望,也是教师对自身身份认同的价值选择。在古典视域中,教师角色根源于对真理的追求、对文化的接续、对责任的持守。然而,在现代性浪潮的裹挟下,理性的自负、价值追... 详细信息

教师角色是教师在教育领域开展教学的形象预设,它承载着社会对教师的期望,也是教师对自身身份认同的价值选择。在古典视域中,教师角色根源于对真理的追求、对文化的接续、对责任的持守。然而,在现代性浪潮的裹挟下,理性的自负、价值追求的异化、学校公共性的丧失,导致古典教师角色呈现现代性异化。美国哲学家汉娜·阿伦特的教育思想对解决这一问题有着重要的借鉴意义,因此,教师角色应汲取其哲学智慧,为实现其复归探寻可能路径。

关键词：古典教师角色现代性汉娜·阿伦特

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挠性卫星姿态运动的鲁棒控制技术

厦门大学学报（自然科学版） 2001年第2期40卷 211-219页

作者：陈亚陵厦门大学自动化系福建厦门361005

具有挠性振动和液体晃动的新一代挠性卫星姿态运动是一个不确定系统 ,运用 H∞ 优化与 μ综合技术研究具有抑制振动和晃动能力以及强鲁棒性的卫星姿态控制系统设计问题 ,并对所提出的几种鲁棒控制系统设计方案进行计算机仿真和全物理仿... 详细信息

具有挠性振动和液体晃动的新一代挠性卫星姿态运动是一个不确定系统 ,运用 H∞ 优化与 μ综合技术研究具有抑制振动和晃动能力以及强鲁棒性的卫星姿态控制系统设计问题 ,并对所提出的几种鲁棒控制系统设计方案进行计算机仿真和全物理仿真实验研究 .

关键词：卫星姿态运动鲁棒控制挠性振动回路成形物理仿真液体晃动姿态控制航天技术

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“倒计时”制度:学校时间观念的批判性反思

教育理论与实践 2017年第16期37卷 28-31页

作者：陈亚凌华中师范大学教育学院湖北武汉430079

"倒计时"制度藉由提示特殊事件的到来,深切地影响着每一个学生的学习生活。它通过塑造心灵和驯化行为的方式将学校时间观念加速传递,使学生异化为时间的奴隶。其实,"倒计时"制度的盛行是由于效率主义的覆盖、制度教育的紧缩、教师定位... 详细信息

"倒计时"制度藉由提示特殊事件的到来,深切地影响着每一个学生的学习生活。它通过塑造心灵和驯化行为的方式将学校时间观念加速传递,使学生异化为时间的奴隶。其实,"倒计时"制度的盛行是由于效率主义的覆盖、制度教育的紧缩、教师定位的偏差等诸多方面的原因导致的。为超脱这一困境,有必要重申时间制度的属人立场,促进时间制度的视域转换,构建时间制度的革新保障。

关键词： “倒计时”制度学校时间观念塑造心灵驯化行为效率主义制度教育教师定位