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抗IL-5纳米抗体筛选及活性检测

中国生物工程杂志 2024年第2期44卷 59-68页

作者：李诗洁代维燕王雪莲刘畅梁耀极白仲虎陈永奇江南大学粮食发酵工艺与技术国家工程实验室无锡214122 珠海瑞思普利医药科技有限公司珠海519040

白介素-5(IL-5)是一种同源二聚体细胞因子,是嗜酸性粒细胞(eosinophilic,EOS)增殖、活化和成熟的重要调节因子。抗IL-5单克隆抗体能阻断IL-5与IL-5受体亚单位α(IL-5Rα)结合,已成功用于治疗嗜酸性粒细胞哮喘。目前上市的单克隆抗体药... 详细信息

白介素-5(IL-5)是一种同源二聚体细胞因子,是嗜酸性粒细胞(eosinophilic,EOS)增殖、活化和成熟的重要调节因子。抗IL-5单克隆抗体能阻断IL-5与IL-5受体亚单位α(IL-5Rα)结合,已成功用于治疗嗜酸性粒细胞哮喘。目前上市的单克隆抗体药物需要反复注射给药,严重影响了患者的依从性,而且注射给药的全身暴露率高。为获得适合吸入给药的抗体,在羊驼天然库中通过3轮淘选,挑取单克隆通过Phage ELISA初筛,共获得461个阳性克隆,其中50个为序列独特的分子,最终选择30个分子进行重组表达纯化。通过ELISA结合、ELISA阻断、FACS阻断、TF-1增殖抑制等实验对候选抗体进行体外活性检测,成功获得一个具有阻断IL-5和IL-5Rα结合的纳米抗体AIL-A96-Fc。通过与人和猴IL-5的ELISA结合实验表明,该分子具有良好人猴交叉活性,而且在FACS阻断实验和ELISA阻断实验中,AIL-A96-Fc表现出良好的阻断效果。该开发方法不仅提供了一个靶向IL-5的候选纳米抗体AIL-A96-Fc,也为后续开发更多靶向IL-5的候选纳米抗体提供了指导意义。

关键词：白介素-5 纳米抗体阻断活性噬菌体展示哮喘

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沸石增强砂土微生物固化效果研究

辽宁工程技术大学学报（自然科学版） 2024年第3期43卷 304-309页

作者：朱文羲邓华锋李建林熊雨程雷黄小芸陈勇琪三峡库区地质灾害教育部重点实验室湖北宜昌443002 三峡大学土木与建筑学院湖北宜昌443002

为提高砂土的微生物固化效果,考虑沸石作为吸附材料具有多孔的特性,通过宏观物理力学试验和微细观检测,系统分析了沸石对微生物固化砂土的增强效果。研究结果表明:沸石能够显著提高砂土微生物加固过程中的固菌率。与常规微生物固化相比... 详细信息

为提高砂土的微生物固化效果,考虑沸石作为吸附材料具有多孔的特性,通过宏观物理力学试验和微细观检测,系统分析了沸石对微生物固化砂土的增强效果。研究结果表明:沸石能够显著提高砂土微生物加固过程中的固菌率。与常规微生物固化相比,掺入沸石后,固菌率提升约5.5倍,抗压强度提升39.35%,渗透系数减小71.94%。掺入沸石,一方面能够增加碳酸钙沉淀生成量,并改善其分布均匀性;另一方面,沸石周围的碳酸钙沉淀对两侧砂颗粒起到良好的桥接作用,能够增强砂颗粒之间的结构性及试样的整体性。研究成果为进一步优化微生物诱导碳酸钙沉积技术提供参考。

关键词：微生物诱导碳酸钙沉积沸石固菌率微生物固化碳酸钙生成量

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一种基于频率选择的输电线路故障行波测距方法

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电气技术 2024年第2期25卷 31-36,44页

作者：周志通陈永琦黄璜蒋亮大唐水电科学技术研究院有限公司成都610036 西南石油大学电气信息学院成都610500

为解决行波测距法中行波频率过高使测距所需波头幅值降低,进而导致测距失败的问题,本文通过分析频率对行波波头幅值的影响规律,提出一种基于频率选择的故障行波测距方法。首先对故障行波信号做经验模态分解(EMD),获得不同频率段的固有... 详细信息

为解决行波测距法中行波频率过高使测距所需波头幅值降低,进而导致测距失败的问题,本文通过分析频率对行波波头幅值的影响规律,提出一种基于频率选择的故障行波测距方法。首先对故障行波信号做经验模态分解(EMD),获得不同频率段的固有模态函数(IMF)分量,同时对IMF分量做Hilbert变换和一阶微分处理,提取并绘制不同高频IMF分量对应的幅值-时间曲线,通过选择波头幅值较大且频率相对低的高频IMF分量对行波波头进行检测,在保证波头检测精度的前提下,减少因行波频率过高导致信号衰减加剧的影响,最后结合三端行波测距算法完成故障测距。仿真结果表明,本文所提方法的相对误差在±0.3%以内,绝对误差在±0.9km以内,与传统的瞬时频率定位方法相比,具有更高测距精度。

关键词：故障测距频率选择瞬时振幅输电线路

DOPO基反应型阻燃剂的制备及其阻燃热塑性聚氨酯

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工程塑料应用 2024年第2期52卷 142-147页

作者：齐鸣张文佳单德鹏王金龙彭正杨承刚陈泳齐宋佳音刘鲁斌国网黑龙江省电力有限公司哈尔滨150040 黑龙江省阻燃材料分子设计与制备重点实验室哈尔滨150040 国网四川电力送变电建设有限公司成都610000 东北林业大学计算机与控制工程学院哈尔滨150040

以10-(2,5-二羟基苯基)-10-氢-9-氧杂-10-磷杂菲-10-氧化物(DOPO-HQ)和甲苯二异氰酸酯(TDI)为原料,通过加成反应成功制备了含端异氰酸酯基的DOPO基反应型阻燃剂(DRFR),并将其引入到热塑性聚氨酯(TPU)基体中,从而制备阻燃TPU复合材料,并... 详细信息

以10-(2,5-二羟基苯基)-10-氢-9-氧杂-10-磷杂菲-10-氧化物(DOPO-HQ)和甲苯二异氰酸酯(TDI)为原料,通过加成反应成功制备了含端异氰酸酯基的DOPO基反应型阻燃剂(DRFR),并将其引入到热塑性聚氨酯(TPU)基体中,从而制备阻燃TPU复合材料,并对TPU/DRFR复合材料的阻燃性能、燃烧行为、热降解性能和力学性能进行研究。结果表明,当DRFR的质量分数为26%时,TPU/DRFR复合材料的阻燃等级达到UL 94 V-0级,极限氧指数达27.6%,且熔滴得到了明显的抑制。DRFR的引入促进了TPU基体的提前降解炭化,从而使TPU/DRFR复合材料表面形成了致密、均一且坚硬的炭层,其有效地在固相中发挥了“挡板”作用,使得TPU/DRFR复合材料的热释放速率峰值和总热释放量与纯TPU相比分别降低了49.64%和34.65%。与此同时,DRFR在分解过程中,产生的含磷自由基和惰性气体有效地在气相中发挥了抑制作用,从而使得TPU/DRFR复合材料获得了优异的火安全性能。此外,DRFR的引入对TPU分子链段起到了交联作用,显著增强了TPU复合材料的拉伸强度。

关键词：热塑性聚氨酯 DOPO基反应型阻燃剂阻燃性能力学性能

Interval analysis using least squares support vector fuzzy regression

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控制理论与应用（英文版） 2012年第4期10卷 458-464页

作者： yongqi chen Qijun chen College of Science and Technology Ningbo University Department of Control Science and Engineering Tongji University

A least squares support vector fuzzy regression model （LS-SVFR） is proposed to estimate uncertain and imprecise data by applying the fuzzy set principle to weight vectors. This model only requires a set of linear equations to obtain the weight vector and the bias term, which is different from the solution of a complicated quadratic programming problem in existing support vector fuzzy regression models. Besides, the proposed LS-SVFR is a model-free method in which the underlying model function doesn＇t need to be predefined. Numerical examples and fault detection application are applied to demonstrate the effectiveness and applicability of the proposed model.

关键词： Interval analysis Least squares Fuzzy regression Fuzzy sets

Acupotomy alleviates knee osteoarthritis in rabbit by regulating chondrocyte mitophagy via Pink1-Parkin pathway

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Journal of Traditional Chinese Medicine 2024年第3期44卷 468-477页

作者： ZHU Wenting GUO Changqing DU Mei MA Yunxuan CUI yongqi chen Xilin GUO Changqing the Third Affiliated Hospital of Beijing University of Chinese Medicine School of Acupuncture-Moxibustion and Tuina Beijing University of Chinese Medicine Department of Medical Technology Shijiazhuang Medical College

OBJECTIVE: To investigate the effect of acupotomy, on mitophagy and the Pink1-Parkin pathway in chondrocytes from rabbits with knee osteoarthritis(KOA).METHODS: A KOA model was established via the modified Videman method. Rabbits were randomly divided into a control group(CON), KOA group and KOA + acupotomy group(Acu). Rabbits in the acupotomy group were subjected to acupotomy for 4 weeks after model establishment. The behavior of the rabbits before and after intervention was recorded. Cartilage degeneration was evaluated by optical microscopy and fluorescence microscopy. The level of mitophagy was evaluated by transmission electron microscopy,immunofluorescence and enzyme-linked immunosorbent assay(ELISA). The expression of phosphatase and tensin homolog(PTEN)-induced kinase 1(Pink1)-Parkin mitophagy pathway components was evaluated by immunofluorescence, Western blotting and real-time polymerase chain ***: In rabbits with KOA, joint pain, mobility disorders and cartilage degeneration were observed, the Mankin score was increased, collagen type Ⅱ(Col-Ⅱ)expression was significantly decreased, mitophagy was inhibited, mitochondrial function was impaired, and factors associated with the Pink1-Parkin pathway were inhibited. Acupotomy regulated the expression of Pink1-Parkin pathway-related proteins, the mitophagy-related protein microtubule-associated protein-1 light chain-3,the translocase of the outer membrane, and the inner mitochondrial membrane 23; increased the colocalization of mitochondria and autophagosomes; promoted the removal of damaged mitochondria;restored mitochondrial adenosine-triphosphate(ATP) production;and alleviated cartilage degeneration in rabbits with ***: Acupotomy played a role in alleviating KOA in rabbits by activating mitophagy in chondrocytes via the regulation of proteins that are related to the Pink1-Parkin pathway.

关键词： PTEN phosphohydrolase acupuncture therapy cartilage mitophagy osteoarthritis, knee

Structural vibration passive control and economic analysis of a high-rise building in Beijing

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Earthquake Engineering and Engineering Vibration 2009年第4期8卷 561-568页

作者： chen yongqi Cao Tiezhu Ma Liangzhe Luo Chaoying Beijing Qitai Shock Control and Scientific Development Co. Ltd Beijing 10003 7 China John A. Martin ＆ Associates Inc. Los Angeles USA

Performance analysis of the Pangu Plaza under earthquake and wind loads is described in this paper. The plaza is a 39-story steel high-rise building, 191 m high, located in Beijing close to the 2008 Olympic main stadium. It has both fluid viscous dampers （FVDs） and buckling restrained braces or unbonded brace （BRB or UBB） installed. A repeated iteration procedure in its design and analysis was adopted for optimization. Results from the seismic response analysis in the horizontal and vertical directions show that the FVDs are highly effective in reducing the response of both the main structure and the secondary system. A comparative analysis of structural seismic performance and economic impact was conducted using traditional methods, i.e., increased size of steel columns and beams and/or use of an increased number of seismic braces versus using FVD. Both the structural response and economic analysis show that using FVD to absorb seismic energy not only satisfies the Chinese seismic design code for a ＂rare＂ earthquake, but is also the most economical way to improve seismic performance both for one-time direct investment and long term maintenance.

关键词： fluid viscous dampers (FVD) damping ratio secondary system One-time investment maintenance factor

Sentiment Analysis Using E-Commerce Review Keyword-Generated Image with a Hybrid Machine Learning-Based Model

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Computers, Materials & Continua 2024年第7期80卷 1581-1599页

作者： Jiawen Li Yuesheng Huang Yayi Lu Leijun Wang yongqi Ren Rongjun chen School of Computer Science Guangdong Polytechnic Normal UniversityGuangzhou510665China Hubei Province Key Laboratory of Occupational Hazard Identification and Control Wuhan University of Science and TechnologyWuhan430065China

In the context of the accelerated pace of daily life and the development of e-commerce,online shopping is a mainstreamway for consumers to access products and *** understand their emotional expressions in facing different shopping experience scenarios,this paper presents a sentiment analysis method that combines the ecommerce reviewkeyword-generated imagewith a hybrid machine learning-basedmodel,inwhich theWord2Vec-TextRank is used to extract keywords that act as the inputs for generating the related images by generative Artificial Intelligence(AI).Subsequently,a hybrid Convolutional Neural Network and Support Vector Machine(CNNSVM)model is applied for sentiment classification of those keyword-generated *** method validation,the data randomly comprised of 5000 reviews from Amazon have been *** superior keyword extraction capability,the proposedmethod achieves impressive results on sentiment classification with a remarkable accuracy of up to 97.13%.Such performance demonstrates its advantages by using the text-to-image approach,providing a unique perspective for sentiment analysis in the e-commerce review data compared to the existing ***,the proposed method enhances the reliability and insights of customer feedback surveys,which would also establish a novel direction in similar cases,such as social media monitoring and market trend research.

关键词： Sentiment analysis keyword-generated image machine learning Word2Vec-TextRank CNN-SVM

基于人工智能的肺磨玻璃密度结节三维测量与病理切片测量的相关性研究

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中国中西医结合影像学杂志 2024年第4期22卷 371-374,378页

作者：吴瑜张莲杨玉婵丁晓青张冬晴陈永其詹松华张敏上海中医药大学附属曙光医院影像医学科上海201203 上海市嘉定区中医医院放射科上海201800 上海中医药大学附属曙光医院病理科上海201203

目的:分析肺磨玻璃密度结节人工智能测量、半自动测量与病理数字切片测量之间的相关性。方法:收集91例表现为磨玻璃密度结节的T1a期肺腺癌的CT图像及病理切片资料。使用人工智能肺部影像分析系统(AILIAS)及半自动分割技术(SLLS)测量CT... 详细信息

目的:分析肺磨玻璃密度结节人工智能测量、半自动测量与病理数字切片测量之间的相关性。方法:收集91例表现为磨玻璃密度结节的T1a期肺腺癌的CT图像及病理切片资料。使用人工智能肺部影像分析系统(AILIAS)及半自动分割技术(SLLS)测量CT图像上结节的平均直径和体积,并在病理数字切片上测量结节的平均直径与面积。AILIAS及SLLS与病理数字切片平均直径测量值、AILIAS与SLLS体积测量值两两比较行Wilcoxon符号秩检验。采用Spearman秩相关分析AILIAS及SLLS体积测量值与病理数字切片面积测量值的相关性。结果:AILIAS与SLLS所测平均直径均大于病理数字切片(Z=-7.310,-8.557;均P<0.001)。AILIAS与SLLS所测直径间差异无统计学意义(Z=-0.744,P=0.457)。SLLS所测体积小于AILIAS(Z=-6.218,P<0.001)。AILIAS和SLLS体积测量值与病理数字切片面积测量值均显著相关(rs=0.729,0.727;均P<0.001)。结论:人工智能可多维度测量肺磨玻璃结节的直径和体积,与病理数字切片面积测量值有良好的相关性及一致性,可为磨玻璃结节的良恶性判定及临床疗效评估提供数据支持。

关键词：肺腺癌磨玻璃密度结节体层摄影术,X线计算机肺结节分割肺结节三维测量