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Yolk free egg substitute improves the serum phospholipid profile of mice with metabolic syndrome based on lipidomic analysis

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Food Science and Human Wellness 2024年第1期13卷 482-493页

作者： Zhihui Yu Lingyu Fan Fei Tai Lixin Zhang Xiaoyu Zhang yisheng chen College of Food Science and Engineering Shanxi Agricultural UniversityTaigu 030801China Insitute of Food Nutrition and Safety Shanxi Agricultural UniversityTaiyuan 030031China Shanxi Key Laboratory of Edible Fungi for Loess Plateau Taigu 030801China

In this study,the impacts of egg consumption on mice model of metabolic syndrome(Met S)were comparatively *** were divided into five groups(n=8):normal diet group(ND),high-fat diet group(HFD),HFD with whole egg group(WE),HFD with free-yolk egg substitute group(YFES),and HFD with lovastatin group(Lov).Main biochemical indexes and a non-targeted lipidomic analysis were employed to insight the lipid profile changes in *** was revealed that WE could significantly improve serum biochemical indexes by reducing body weight,low-density lipoprotein cholesterol(LDL-C)and total cholesterol(TC),while increasing high-density lipoprotein *** exhibited remarkably better performance in increasing phosphatidylglycerol and phosphatidic acids,while decreasing phosphatidylinositol than WE.A total of 50 differential lipids biomarkers tightly related to glycerophospholipids metabolism were screened *** C18:2 and C12:1,SM(d18:0/12:0),and SM(d18:1/14:1)were significantly upregulated in YFES compared to *** reduced expression of SREBP-1c and Cpt1a,while did not affect the expression of PPAR-α.Sphingomyelin biomarkers were positively related to the TC(|r|>0.6),while PPAR-αwas negatively correlated with triglyceride and LDL-C *** sum up,YFES attenuated HFD-induced Met S by improving the serum phospholipids,which account for its modulation of glycerophospholipid metabolism.

关键词： Metabolic syndrome Whole egg Yolk free egg substitute Serum Lipidomics

Facile SERS screening of melamine in bovine milk with 2D printed AgNPs/glass fabric filter paper as the flexible substrate

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Food Science and Human Wellness 2024年第5期13卷 2595-2600页

作者： Shiyao Wang Yanan Zhao Xiao Wei yisheng chen College of Food Science and Engineering Shanxi Agricultural UniversityTaigu 030801China Food Nutrition and Safety Institute Shanxi Agricultural UniversityTaiyuan 030031China School of Food Science and Technology Jiangnan UniversityWuxi 214122China

Melamine is one of the most frequently detected adulterants in dairy *** current study proposes a surface-enhanced Raman spectroscopy(SERS)-based analytical tool for fast and reliable screening of melamine in bovine milk.A hand-held Raman spectrometer was used in conjunction with a substrate composed of silver nanoparticles(AgNPs)that was 2D printed onto glass fiber(GF)filter *** optimized conditions,a sensitive and fingerprint-like signal at 674 cm^(-1) was *** AgNPs/GF substrate exhibited high sensitivity to melamine in milk down to 1.9498×10^(-5)mg/mL,which is well below the USA and EU safety limits(2.5×10^(-3)mg/mL).Remarkably,the proposed technology was also highly reproducible,showing spot-to-spot and block-to-block variations below 3.3%and 4.9%at 674 cm^(-1) in Raman intensity,*** characteristic peak intensity and concentration of melamine showed an acceptable linear relationship(R^(2)=0.9909)within the range of 0.0001-1 mg/***,the method established in this study can provide an efficient and effective method for the quantitative target screening and detection of melamine in dairy products.

关键词： 2D print Melamine Milk Surface-enhanced Raman spectroscopy(SERS) flexible substrate

Effects of phosphorylated ovalbumin on the quality of pork myofibrillar protein gel:an insight into gelling and physicochemical properties

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Journal of Future Foods 2024年第2期4卷 149-158页

作者： Zhihui Yu Yating Gao Meng Wu Chaofan Zhao Xiubin Liu Xiaoyu Zhang Lixin Zhang yisheng chen College of Food Science and Engineering Shanxi Agricultural UniversityTaigu 030801China College of Animal Science and Technology Hunan Agricultural UniversityChangsha 410128China Houji Laboratory in Shanxi Province Shanxi Agricultural UniversityTaiyuan 030031China

This study aimed to investigate the effects of phosphorylated-ovalbumin(P-OVA)at different concentrations(0.5%and 1.0%)on the gel properties of pork myofibrillar protein(MP).The results showed that the textural properties such as gel hardness,cohesiveness,springiness and chewiness were improved with P-OVA addition at 0.5%.The water holding capacity(up to 75.89%)and gel strength(up to 168.56 g·mm)of MP gel were markedly increased after P-OVA *** absolute value of zeta potential reached 13.85 mV and maximum hydrophobicity(15.2μg)resulted from the addition of 0.5%*** storage modulus(G’)and loss modulus(G’’)of MP gel were significantly increased from 50°C,and the G’and G’’significantly increased after 1.0%P-OVA addition,evidencing that the cross-linking effect of MP protein gel was *** addition,the P-OVA addition improved the structure of MP gel protein by reducing theα-helix,while increasing theβ-sheet and the r-value(the ratio between two ultraviolet second-derivative peak-to-trough distances),which further promoted the uniform and compact gel network *** work demonstrated that P-OVA is a highly effective modifier that significantly improves the quality of MP *** a view of practice,0.5%P-OVA is the optimal addition amount.

关键词： Phosphorylated ovalbumin Myofibrillar proteins Gel properties Structures

Insight into effects of egg- and soybean-derived phosphatidylcholine on non-alcoholic steatosis in mice and HepG2 cells by lipidomics

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Food Science and Human Wellness 2024年

作者： Zhihui Yu Chunyou Liu Yating Gao Haoran Guo Huiling Duan Lixin Zhang yisheng chen College of Food Science and Engineering， Shanxi Agricultural University Institute of Food Nutrition and Safety， Shanxi Agricultural University Department of Food Science and Technology， Guangxi University of Science and Technology

Dietary phosphatidylcholine (PC) is an ideal source of bioactive lipids showing efficacy to alleviate non-alcoholic fatty liver disease (NAFLD). In this study， the lipidomic profiles of nonalcoholic steatosis in mice and HepG2 cells treated with PC isolated from eggs (EPC) and soybean (SPC) were evaluated. EPC and SPC could significantly ameliorate fatty liver disease by down-regulating triglyceride (TG) and diglyceride (DG) levels. Remarkably， EPC exhibited better performance in regulating the hepatic lipid profile leading to decreased PC and TG， ceramides， phosphatidylethanolamine and phosphatidylserine levels. A total of 117 and 20 lipid biomarkers in liver and HepG2 cells involved in glycerophospholipid metabolism were screened out， respectively. The regulatory mechanism of EPC may be attributed to a decrease in the expression of ACACA and SREBP-1c. EPC， via suppressing lipogenic gene expression， which was superior to that of SPC in alleviating nonalcoholic steatosis. Overall， EPC and SPC attenuated HFD-induced NAFLD by improving the hepatic lipid profile. The more significant effect observed with EPC may be attributed to its modulation of glycerophospholipid metabolism.

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HPTLC-fluorescent densitometry for screening aflatoxin B1 in millet and buckwheat

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Food Science and Human Wellness 2024年

作者： Xudong Shi Xingjun Xi Yuetao Jia Zhijian Wang Jiawei Guo Shiyao Wang Xiaoqian Tang yisheng chen College of Food Science and Engineering， Shanxi Agricultural University China National Institute of Standardization Key Laboratory of Detection for Mycotoxins， Ministry of Agriculture and Rural Affairs Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences

Given severe health-hazardous effects of aflatoxin B1 (AFB1) widely occurring in cereal grains and animal feeds， it is highly urgent to develop analytical methods for its rapid screening. In this work， we proposed a simple and high-throughput method for the determination of AFB1 in millet and buckwheat samples using high performance thin layer chromatography (HPTLC) linked to fluorescence densitometry. The first step was to optimize the solid-liquid extraction for the crude clean-up of the samples. The QuEChERS (Quick， Easy， Cheap， Effective， Robust and Safe) extraction strategy was used and different solvent systems for their extraction efficiency of AFB1 from the samples were evaluated. Then， trichloromethane/ethyl acetate (7/3， v/v) was used as the mobile phase to realize the separation of the targeted compound from background noises on silica gel plates. Quantification was readily performed with densitometry in fluorescence mode. In order to fix the optimal excitation wavelength， spectra scanning from 250-400 nm was carried out， revealing that 364 nm light gave the highest signal. With the optimized optical system， high sensitivity to AFB1 was achieved， with a limit of detection (LOD) at 3 μg/kg. Apart from that， good linearity (0.999) was obtained within the range of 1-80 ng/band of AFB1. To assess the analysis accuracy， two levels of AFB1 (50 and 100 μg/kg) were spiked into real grain samples. The obtained results showed that the recovery rates were within the range of (81.6–114.0)%. The proof-of-concept results of this work evidenced that HPTLC is a promising analytical tool for the screening of mycotoxin in difficult samples.

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Impacts of Nutlin-3a and exercise on murine double minute 2-enriched glioma treatment

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Neural Regeneration Research 2025年第4期20卷 1135-1152页

作者： yisheng chen Zhongcheng Fan Zhiwen Luo Xueran Kang Renwen Wan Fangqi Li Weiwei Lin Zhihua Han Beijie Qi Jinrong Lin Yaying Sun Jiebin Huang Yuzhen Xu Shiyi chen Department of Sport Medicine Huashan HospitalFudan UniversityShanghaiChina Department of Orthopedic Surgery Hainan Province Clinical Medical CenterHaikou Affiliated Hospital of Central South University Xiangya School of MedicineHaikouHainan ProvinceChina Department of Basic Medicine Shanghai Jiao Tong University School of MedicineShanghai Jiao Tong UniversityShanghaiChina Department of Neurosurgery Second Affiliated Hospital of Zhejiang University School of MedicineZhejiang UniversityHangzhouZhejiang ProvinceChina Department of Orthopedics Shanghai General HospitalSchool of Medicine Shanghai Jiao Tong UniversityShanghaiChina Department of Infectious Diseases Children’s Hospital of Fudan UniversityNational Children’s Medical CenterShanghaiChina Department of Rehabilitation The Second Affiliated Hospital of Shandong First Medical UniversityTaianShandong ProvinceChina

Recent research has demonstrated the impact of physical activity on the prognosis of glioma patients,with evidence suggesting exercise may reduce mortality risks and aid neural *** role of the small ubiquitin-like modifier(SUMO)protein,especially post-exercise,in cancer progression,is gaining attention,as are the potential anti-cancer effects of *** used machine learning to create the exercise and SUMO-related gene signature(ESLRS).This signature shows how physical activity might help improve the outlook for low-grade glioma and other *** demonstrated the prognostic and immunotherapeutic significance of ESLRS markers,specifically highlighting how murine double minute 2(MDM2),a component of the ESLRS,can be targeted by *** underscores the intricate relationship between natural compounds such as nutlin-3 and immune *** comprehensive CRISPR screening,we validated the effects of specific ESLRS genes on low-grade glioma *** also revealed insights into the effectiveness of Nutlin-3a as a potent MDM2 inhibitor through molecular docking and dynamic ***-3a inhibited glioma cell proliferation and activated the p53 *** efficacy decreased with MDM2 overexpression,and this was reversed by Nutlin-3a or *** using a low-grade glioma mouse model highlighted the effect of physical activity on oxidative stress and molecular pathway ***,both physical exercise and Nutlin-3a administration improved physical function in mice bearing tumors derived from MDM2-overexpressing *** results suggest the potential for Nutlin-3a,an MDM2 inhibitor,with physical exercise as a therapeutic approach for glioma *** research also supports the use of natural products for therapy and sheds light on the interaction of exercise,natural products,and immune regulation in cancer treatment.

关键词： exercise and SUMO-related gene signatures(ESLRS) glioblastoma management low-grade glioma natural bioactives neural regeneration physical exercise

基于纳米银负载的SERS拭纸制备与果蔬表面农残无损检测应用:以福美铁为例

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食品科学 2024年第10期45卷 265-271页

作者：张超凡赵亚楠王诗瑶张奇陈益胜山西农业大学食品科学与工程学院山西晋中030600 中国农业科学院油料作物研究所农业农村部生物毒素检测重点实验室湖北武汉430000

通过简单的浸泡方法制备可以得到柔性的滤纸表面增强拉曼散射(surface-enhanced Raman scattering,SERS)拭纸,本研究通过改变其合成和保存条件使其SERS检测性能得到优化。优化后的SERS基底在果蔬表面福美铁残留的快速无损检测中表现出... 详细信息

通过简单的浸泡方法制备可以得到柔性的滤纸表面增强拉曼散射(surface-enhanced Raman scattering,SERS)拭纸,本研究通过改变其合成和保存条件使其SERS检测性能得到优化。优化后的SERS基底在果蔬表面福美铁残留的快速无损检测中表现出优异性能。研究表明:将滤纸基底在不同浓缩倍数的纳米银中分别浸泡不同的时间,并采用不同的干燥方法,可对SERS基底的检测性能产生显著影响。其中,将滤纸于5倍浓缩纳米银溶胶中浸泡8 h制备所得SERS基底性能明显优于浸泡其余不同时长和浓缩倍数纳米银溶胶制备的基底。采用真空干燥所制备的SERS基底相较于其他干燥方式所得的基底性能更加优秀。在-20℃保存条件下,优化后的基底在保存9 d后信号值仍能达到原始峰值的50%左右。同时优化后的滤纸基底具有良好的灵敏性、均匀性和重复性,在玻璃板上检测福美铁的检出限(limit of detection,LOD)可达4.07×10^(-6)mg/m L,在菠菜、油桃表面对福美铁的LOD分别是0.043 mg/kg和0.062 mg/kg。该研究可为滤纸SERS基底的开发利用提供有效依据。

关键词：柔性基底表面增强拉曼散射农药残留福美铁

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智能建造机器人局部路径规划研究综述

计算机工程与应用 2024年第10期60卷 16-29页

作者：于军琪陈易圣冯春勇苏煜聪郭聚刚西安建筑科技大学信息与控制工程学院西安710055 西安建筑科技大学机电工程学院西安710055 西安建筑科技大学建筑设备科学与工程学院西安710055

智能建造机器人作为落实建筑业智能化转型的关键设备,其自主施工能力代表着智能建造的水平,而局部路径规划是机器人高效施工的关键技术。针对建筑施工现场的环境特点,探究了智能建造机器人局部路径规划的难点。首先分析阐述了人工势场... 详细信息

智能建造机器人作为落实建筑业智能化转型的关键设备,其自主施工能力代表着智能建造的水平,而局部路径规划是机器人高效施工的关键技术。针对建筑施工现场的环境特点,探究了智能建造机器人局部路径规划的难点。首先分析阐述了人工势场、时间弹性带、Bug、动态窗口等经典局部路径规划算法;其次对强化学习、深度强化学习、模糊控制、群智能等人工智能的局部路径规划算法进行了归纳总结;最后分析了各种方法在施工现场环境中智能建造机器人应用的局限性,讨论了智能建造机器人局部路径规划算法的发展趋势,旨在为复杂施工现场智能建造机器人局部路径规划研究提供一定的思路和建议,提高机器人的自主施工能力,促进智能建造技术的发展。

关键词：智能建造建筑机器人局部路径规划经典规划算法人工智能

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镧-铈混合添加对A356铝合金的变质研究

特种铸造及有色合金 2024年第10期44卷 1388-1392页

作者：邢博岩陈义胜闫永旺刘艺畅内蒙古科技大学材料与冶金学院包头014017

采用金相显微镜、钨灯丝扫描电镜、场发射扫描电镜等手段,研究了0~0.35%的La、Ce混合稀土添加对A356铝合金的微观组织和力学性能的影响。结果表明,当混合稀土添加量为0.35%时,A356铝合金的组织与力学性能最好,α-Al晶粒等效直径和二次... 详细信息

采用金相显微镜、钨灯丝扫描电镜、场发射扫描电镜等手段,研究了0~0.35%的La、Ce混合稀土添加对A356铝合金的微观组织和力学性能的影响。结果表明,当混合稀土添加量为0.35%时,A356铝合金的组织与力学性能最好,α-Al晶粒等效直径和二次枝晶臂间距(SDAS)均达到最小值,分别为24.24μm和38.07μm,共晶Si细化理想,抗拉强度和屈服强度分别达到178.5 MPa、145.6MPa,断裂类型由脆性断裂逐渐变为韧性断裂。

关键词： A356铝合金混合稀土微观组织力学性能

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