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Status Quo of Biodiversity in Ta’e Basin of Xinjiang and Its Maintenance Function

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Meteorological and Environmental Research 2019年第5期10卷 59-62页

作者： chen Li Li Xiaoran Xinjiang Academy of Environmental Protection Sciences Urumqi 830011China Xinjiang Key Laboratory of Environmental Pollution Monitoring and Risk Early Warning Urumqi 830011China Xinjiang Cleaner Production Engineering Technology Research Center Urumqi 830011China National Environmental Protection Scientific Observation and Research Station of Junggar Desert-Oasis Ecotone Urumqi 830011China

For status quo of biodiversity in Ta'e Basin of Xinjiang and assessment on biodiversity maintenance ability of ecosystem,overall situation of biodiversity and existing problems in Ta'e Basin are analyzed,and regional measures and suggestions for biodiversity protection are proposed,which could provide theoretical basis for regional eco-environment protection and management.

关键词： Ta'e Basin Biodiversity Service function

Lycium barbarum glycopeptide(wolfberry extract)slows N-methyl-N-nitrosourea-induced degradation of photoreceptors

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Neural Regeneration Research 2024年第10期19卷 2290-2298页

作者： Qihang Kong Xiu Han Haiyang cheng Jiayu Liu Huijun Zhang Tangrong Dong Jiansu chen Kwok-Fai So Xuesong Mi Ying Xu Shibo Tang Department of Ophthalmology Aier Eye HospitalJinan UniversityGuangzhouGuangdong ProvinceChina Department of Ophthalmology The First Affiliated Hospital of Jinan UniversityGuangzhouGuangdong ProvinceChina Guangdong-Hong Kong-Macao Institute of CNS Regeneration Key Laboratory of CNS Regeneration(Ministry of Education)Jinan UniversityGuangzhouGuangdong ProvinceChina Department of Ophthalmology Guangzhou Panyu Central HospitalGuangzhouGuangdong ProvinceChina School of Stomatology Jinan UniversityGuangzhouGuangdong ProvinceChina Aier Academician Station ChangshaHunan ProvinceChina Co-Innovation Center of Neuroregeneration Nantong UniversityNantongJiangsu ProvinceChina State Key Laboratory of Brain and Cognitive Sciences Hong Kong Special Administrative RegionChina

Photoreceptor cell degeneration leads to blindness, for which there is currently no effective treatment. Our previous studies have shown that Lycium barbarum(L. barbarum) polysaccharide(LBP) protects degenerated photoreceptors in rd1, a transgenic mouse model of retinitis pigmentosa. L. barbarum glycopeptide(Lb GP) is an immunoreactive glycoprotein extracted from LBP. In this study, we investigated the potential protective effect of Lb GP on a chemically induced photoreceptor-degenerative mouse model. Wild-type mice received the following: oral administration of Lb GP as a protective pre-treatment on days 1–7;intraperitoneal administration of 40 mg/kg N-methylN-nitrosourea to induce photoreceptor injury on day 7;and continuation of orally administered Lb GP on days 8–14. Treatment with Lb GP increased photoreceptor survival and improved the structure of photoreceptors, retinal photoresponse, and visual behaviors of mice with photoreceptor degeneration. Lb GP was also found to partially inhibit the activation of microglia in N-methyl-N-nitrosourea-injured retinas and significantly decreased the expression of two pro-inflammatory cytokines. In conclusion, Lb GP effectively slowed the rate of photoreceptor degeneration in N-methyl-N-nitrosourea-injured mice, possibly through an anti-inflammatory mechanism, and has potential as a candidate drug for the clinical treatment of photoreceptor degeneration.

关键词： anti-inflammation inherited retinal diseases Lycium barbarum glycopeptide N-methyl-N-nitrosourea opsin photoreceptor reactive gliosis retinal degeneration retinitis pigmentosa rhodopsin

Neuroprotective effects of ZL006 in Aβ1–42-treated neuronal cells

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Neural Regeneration Research 2020年第12期15卷 2296-2305页

作者： Wen-Yuan Tao Lin-Jie Yu Su Jiang Xiang Cao Jian chen Xin-Yu Bao Fei Li Yun Xu Xiao-Lei Zhu Department of Neurology Drum Tower HospitalMedical School of Nanjing UniversityNanjingJiangsu ProvinceChina The State Key Laboratory of Pharmaceutical Biotechnology Nanjing UniversityNanjingJiangsu ProvinceChina Jiangsu Key Laboratory for Molecular Medicine NanjingJiangsu ProvinceChina Taizhou People’s Hospital TaizhouJiangsu ProvinceChina Department of Medicinal Chemistry School of PharmacyNanjing Medical UniversityNanjingJiangsu ProvinceChina

Amyloid beta(Aβ)-induced neurotoxicity and oxidative stress plays an important role in the pathogenesis of Alzheimer’s disease(AD).ZL006 is shown to reduce over-produced nitric oxide and oxidative stress in ischemic stroke by interrupting the interaction of neuronal nitric oxide synthase and postsynaptic density protein ***,few studies are reported on the role of ZL006 in *** investigate whether ZL006 exerted neuroprotective effects in AD,we used Aβ1–42 to treat primary cortical neurons and N2a neuroblastoma cells as an in vitro model of *** neurons were incubated with ZL006 or dimethyl sulfoxide for 2 hours and treated with Aβ1–42 or NH3·H2O for another 24 *** results of cell counting Kit-8(CCK-8)assay and calcein-acetoxymethylester/propidium iodide staining showed that ZL006 pretreatment rescued the neuronal death induced by Aβ1–*** and western blot assay were used to detect oxidative stress and apoptosis-related proteins in each group of *** showed that ZL006 pretreatment decreased neuronal apoptosis and oxidative stress induced by Aβ1–*** results of CCK8 assay showed that inhibition of Akt or NF-E2-related factor 2(Nrf2)in cortical neurons abolished the protective effects of ***,similar results were also observed in N2a neuroblastoma ***006 inhibited N2a cell death and oxidative stress induced by Aβ1–42,while inhibition of Akt or Nrf2 abolished the protective effect of *** results demonstrated that ZL006 reduced Aβ1–42-induced neuronal damage and oxidative stress,and the mechanisms might be associated with the activation of Akt/Nrf2/heme oxygenase-1 signaling pathways.

关键词： Akt Alzheimer’s disease amyloid-beta apoptosis heme oxygenase-1 neurotoxicity Nrf2 oxidative stress treatment ZL006

Lithiophilic hyperbranched Cu nanostructure for stable Li metal anodes

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SmartMat 2023年第3期4卷 96-107页

作者： Jianyu chen Xin Qiao Wei Fu Xuran Han Qiang Wu Yizhou Wang Yu Zhang Li Shi Jin Zhao Yanwen Ma State Key Laboratory for Organic Electronics and Information Displays(KLOEID)&Jiangsu Key Laboratory for Biosensors Institute of Advanced Materials(IAM)Nanjing University of Posts&TelecommunicationsNanjingChina Materials Science and Engineering King Abdullah University of Science and Technology(KAUST)ThuwalSaudi Arabia New Energy Technology Engineering Lab of Jiangsu Province School of ScienceNanjing University of Posts&TelecommunicationsNanjingChina Suzhou Vocational Institute of Industrial Technology SuzhouChina

Porous copper(Cu)current collectors are regarded as a promising host for stabilizing lithium(Li)metal anodes but suffer from uncontrollable Li metal deposition due to the intrinsic lithiophobic nature of *** study proposes a vertically aligned Cu host with hyperbranched CuxO nanostructure to provide lithiophilic nucleation sites for homogeneous Li metal ***,the vertically aligned Cu nanostructure dramatically reduces the local current density and brings homogeneous Li‐ion *** lithiophilic hyperbranched CuxO nanostructure with a low nucleation barrier could induce homogeneous Li nucleation and *** a result,the Cu@CuxO nanostructured host exhibits a low nucleation overpotential of 44.3 mV and achieves highly electrochemical reversibility with high Coulombic efficiency of 98.33%in a half‐*** Cu@CuxO nanostructured electrode is capable of working under different current densities varying from 0.5 to 5 mA/cm2 in a symmetric *** assembled full cell coupling of the Li/Cu@CuxO composite anode with the LiFePO4 cathode manifests stable long‐term cycling life at 1 *** study elaborates on the synergistic effect of electrode structure design and interfacial chemistry modification to regulate the Li deposition/dissolution behavior,thus exhibiting remarkable electrochemical performances for next‐generation Li‐metal batteries.

关键词： current collector Li metal anode lithiophilicity low nucleation overpotential vertically aligned structure

鸡胚胎素对小鼠红细胞数量及免疫器官质量的影响

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中国组织工程研究与临床康复 2007年第28期11卷 5507-5510页

作者：张晓红陈正跃邱培勇许建文王璐张光军张积霞王永学郑州卫校微生物与免疫教研室河南省郑州市450003 新乡医学院药物研究室河南省新乡市453003

目的:建立血虚和免疫抑制动物模型,观察鸡胚胎低温提取物对其红细胞造血以及免疫器官质量的影响。方法:实验于2001-04/2002-09在新乡医学院药物研究室完成。①实验材料:健康昆明种小鼠50只,雌雄各半。鸡胚胎素[中国发明专利公开(公告)号... 详细信息

目的:建立血虚和免疫抑制动物模型,观察鸡胚胎低温提取物对其红细胞造血以及免疫器官质量的影响。方法:实验于2001-04/2002-09在新乡医学院药物研究室完成。①实验材料:健康昆明种小鼠50只,雌雄各半。鸡胚胎素[中国发明专利公开(公告)号:CN1748713],符合研究者申报专利时提出的质量检验标准。②鸡胚胎素对血虚模型小鼠红细胞数值及血红蛋白含量的影响:取20只小鼠,随机排列表法分为鸡胚胎素组、模型对照组,10只/组,建立失血性血虚动物模型。失血后24h当红细胞数0.05)。末次给鸡胚胎素后2h与模型对照组比较,鸡胚胎素组红细胞数值、血红蛋白含量均明显升高(t=3.39,P<0.01;t=2.52,P<0.05)。②末次给环磷酰胺2h后与模型对照组比较,鸡胚胎素组、正常对照组的胸腺质量指数和脾脏质量指数均明显升高(t=6.62,P<0.01;t=2.47,P<0.05)。结论:鸡胚胎素灌胃对血虚小鼠具有较好的促红细胞造血功能,同时对环磷酰胺造成的免疫器官质量下降具有明显的增重作用。

关键词：鸡胚胎素鸡免疫红细胞环磷酰胺

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骨髓源性神经样细胞体外存活、增殖的特性

中国组织工程研究与临床康复 2007年第15期11卷 2846-2849页

作者：李涛洪光祥李进陈振兵康皓王发斌冯亚高黄启顺翁雨雄华中科技大学同济医学院附属协和医院手外科湖北省武汉市430022

目的:寻找骨髓间充质干细胞体外诱导分化为神经元样/星形胶质样细胞的有效方法,并评价其体外存活和增殖特性。方法:实验于2005-07/2006-08在华中科技大学同济医学院附属协和医院完成。采用贴壁筛选法分离培养大鼠骨髓间充质干细胞。收集... 详细信息

目的:寻找骨髓间充质干细胞体外诱导分化为神经元样/星形胶质样细胞的有效方法,并评价其体外存活和增殖特性。方法:实验于2005-07/2006-08在华中科技大学同济医学院附属协和医院完成。采用贴壁筛选法分离培养大鼠骨髓间充质干细胞。收集第5代骨髓间充质干细胞单细胞悬液,以1×109L-1置入6孔培养板中,加入DMEM+20%胎牛血清生长培养基,24h后更换生长培养基为DMEM/F12+2%B27+40μg/L碱性成纤维生长因子+20μg/L内皮生长因子诱导分化培养基,诱导10～20d,即采用多因子分阶段逐步诱导方法定向诱导骨髓间充质干细胞分化为神经前体样细胞。更换诱导分化培养基为DMEM/5%胎牛血清生长培养基,分两组进行诱导:一组向神经元样诱导分化:全反式维甲酸连续加样,首次浓度0.5μmol/L,以后每天全量加样;二组向星形胶质样细胞诱导分化:10μg/L血小板衍生生长因子BB首次加样,以后每天半量加样,均连续诱导10～14d,进而分化为神经元样/星形胶质样细胞。应用活细胞计数试剂盒CCK-8检测神经元样/星形胶质样细胞的增殖及存活情况,绘制生长曲线及细胞存活曲线。结果:①碱性成纤维生长因子+表皮生长因子连续诱导7d后骨髓间充质干细胞分化为球团样的神经前体样细胞,巢蛋白表达阳性。②全反式维甲酸、血小板衍生生长因子BB分别连续诱导10d后骨髓间充质干细胞逐步分化出神经元样、星形胶质样细胞,并分别表达纤维丝蛋白200及胶质纤维酸性阳性蛋白。③神经元样细胞早期生长较缓慢,具有一定的增殖能力,六七天后达到高峰,此后增殖能力明显下降,细胞存活率明显下降,十四五天之后细胞存活率下降到7.6%以下,难以传代培养。星形胶质样细胞初期生长慢,5d后生长加速,进入对数生长期,七八天后进入平台期,仍保持较高的细胞存活率。结论:采用多因子分阶段诱导方法可成功诱导骨髓间充质干细胞分化为神经元样/星形胶质样细胞。神经元样细胞存活、增殖能力较差,难以传代,星形胶质样细胞存活、增殖能力较强,可建立传代的二倍体细胞系。

关键词：间充质干细胞细胞因子诱导分化神经元样细胞星形胶质样细胞

Expression of visual system homeobox 1 in human keratoconus

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International Journal of Ophthalmology(English edition) 2019年第2期12卷 201-206页

作者： Ya-Ni Wang Xian-Ning Liu Xiao-Dong Wang Yong Yin Yan chen Xiang-Hua Xiao Kun Xu Xiu-Ping Zhu Xi’an No.1 Hospital Shaanxi Institute of Ophthalmology Shaanxi Key Laboratory of Ophthalmology Clinical Research Center for Ophthalmology Diseases of Shaanxi Province First Affiliated Hospital of Northwestern University

AIM: To investigate the expression of visual system homeobox 1(VSX1) and myofibroblast marker alpha smooth muscle actin(α-SMA) in keratoconus(KC). METHODS: Thirty corneal tissue were collected from KC patients after corneal transplantation and 15 normal donor corneas were obtained. All corneal tissues divided into 4 parts for different detections. Scanning electron microscopy was used to observe the ultrastructure of the specimens. VSX1 and α-SMA localization in cornea tissues was detected using immunofluorescence histochemistry. Reverse transcription-quantitative polymerase chain reaction(RT-qPCR) and Western blot were performed to analyze the expression level of VSX1 and α-SMA. RESULTS: Compared to normal cornea tissue, the collagen fibers in KC stroma were distortional and attenuated and keratocytes were abnormally changed. VSX1 and α-SMA located in the corneal stroma. The mRNA and protein expression level of VSX1 in KC were about 3 times as high as that of normal tissue(P<0.001). α-SMA was hardly expressed in the normal corneas, however, its expression in the KC was about 1.5 times higher than that of the normal corneas(P<0.0001). CONCLUSION: Compared with normal corneal the expression of VSX1 and α-SMA in KC both increased. VSX1 is related to the activation of keratocytes and involved in the pathogenesis of keratoconus.

关键词： visual system homeobox 1 keratoconus alpha smooth muscle actin keratocytes

Comparison of ocular biometric parameters between two swept-source optical coherence tomography devices and Scheimpflug tomography in patients with cataract

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International Journal of Ophthalmology(English edition) 2024年第8期17卷 1437-1446页

作者： Shan Ma cheng Li Jing Sun Jun Yang Kai Wen Xi-Teng chen Fang-Yu Zhao Rong-Yu Gao Fang Tian Tianjin Key Laboratory of Retinal Functions and Diseases Tianjin Branch of National Clinical Research Center for Ocular DiseaseEye Institute and School of OptometryTianjin Medical University Eye HospitalTianjin 300380China Zhengda Guangming Eye Group Weifang 261000Shandong ProvinceChina Binzhou Polytechnic Binzhou 256603Shandong ProvinceChina

AIM:To assess and compare the variations and agreements across different ocular biometric parameters using swept-source optical coherence tomography(SS-OCT)and Scheimpflug tomography in patients diagnosed with ***:This prospective case series was conducted at Tianjin Medical University Eye *** total,212 eyes from 212 patients scheduled for phacoemulsification were *** were evaluated preoperatively using two SSOCT devices(IOLMaster700 and CASIA2)and Scheimpflug tomography(Pentacam).Central corneal thickness(CCT),anterior chamber depth(ACD),aqueous depth(AQD),white-to-white distance(WTW),flat simulated keratometry(Kf),steep simulated keratometry(Ks),mean keratometry(Km),and total corneal keratometry(TKm)were *** correlation coefficient(ICC),95%confidence intervals(CI)and limits of agreement(LoA)widths were conducted to assess differences and correlations between ***:All parameters,except for Ks,were significantly *** comparison revealed no significant differences between keratometry obtained by IOLMaster 700 and *** widths of all paired comparisons for Ks were>0.80 *** for WTW between IOLMaster 700 and CASIA2 and between CASIA2 and Pentacam,other Pearson’s coefficients between devices showed a strong correlation(all r>0.95).The ICC of WTW(ICC=0.438,95%CI 0.167-0.625)showed poor *** reliability of CCT,ACD,and AQD was excellent(all ICC>0.95),whereas that of TKm was good(ICC=0.827,95%CI 0.221-0.939).A significant linear correlation was also observed among ***:The ocular parameters derived from the use of IOLMaster700,CASIA2,and Pentacam exhibit significant discrepancies;as such,measurements from these devices should not be deemed as interchangeable.

关键词： ocular parameters swept-source optical coherence tomography cataract

Genome-wide analysis of soybean DnaJA-family genes and functional characterization of GmDnaJA6 responses to saline and alkaline stress

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The Crop Journal 2023年第4期11卷 1230-1241页

作者： Binshuo Zhang Zhen Liu Runnan Zhou Peng cheng Haibo Li Zhiyang Wang Ying Liu Mingyang Li Zhenqing Zhao Zhenbang Hu Qingshan chen Xiaoxia Wu Ying Zhao College of Agriculture Northeast Agricultural UniversityHarbin 150030HeilongjiangChina

Plant Dna JA proteins act as molecular chaperones in response to environmental *** purpose of this study was to characterize the function and regulatory mechanisms of Dna JA genes in *** expression profiles in various soybean tissues at various stages of development indicated that Gm Dna JAs function in the coordination of stress and plant hormone *** Dna JA6 was identified as a candidate regulator of saline and alkaline stress resistance and Gm Dna JA6 overexpression lines showed increased soybean saline and alkaline *** J interacted with Hsp70,and Gm Hsp70 increased the saline and alkaline tolerance of plants with chimeric soybean hairy roots.

关键词： Soybean Heat shock proteins DnaJ Salt stress Alkali stress