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Non-genetic mechanisms of diabetic nephropathy

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Frontiers of Medicine 2017年第3期11卷 319-332页

作者： Qiuxia Han Hanyu Zhu xiangmei chen Zhangsuo Liu Department of Nephrology Chinese PLA General Hospital Chinese PLA Institute of Nephrology State Key Laboratory of Kidney DiseasesNational Clinical Research Center for Kidney Diseases Beijing Key Laboratory of Kidney Diseases Beijing 100853 China Department of Nephrology The First Affiliated Hospital of Zhengzhou University Zhengzhou 450052 China

Diabetic nephropathy （DN） is one of the most common microvascular complications in diabetes mellitus patients and is characterized by thickened glomeruIar basement membrane, increased extracellular matrix formation, and podocyte loss. These phenomena lead to proteinuria and altered glomerular filtration rate, that is, the rate initially increases but progressively decreases. DN has become the leading cause of end-stage renal disease. Its prevalence shows a rapid growth trend and causes heavy social and economic burden in many countries. However, this disease is multifactorial, and its mechanism is poorly understood due to the complex pathogenesis of DN. In this review, we highlight the new molecular insights about the pathogenesis of DN from the aspects of immune inflammation response, epithelial-mesenchymal transition, apoptosis and mitochondrial damage, epigenetics, and podocyte-endothelial communication. This work offers groundwork for understanding the initiation and progression of DN, as well as provides ideas for developing new prevention and treatment measures.

关键词： diabetic nephropathy immune inflammatory response epithelial-mesenchymal transition apoptosis mitochondrial damage epigenetics podocyte-endothelial communication

Potential use of serum HBV RNA in antiviral therapy for chronic hepatitis B in the era of nucleos（t）ide analogs

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Frontiers of Medicine 2017年第4期11卷 502-508页

作者： Fengmin Lu Jie Wang xiangmei chen Dongping Xu Ningshao Xia State Key Laboratory of Natural and Biomimetic Drugs Department of Microbiology & Infectious Disease Center School of Basic Medical Sciences Peking University Health Science Center Beijing 100191 China Institute of Infectious Diseases Beijing 302 Hospital Beijing 100039 China State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics School of Public Health Xiamen UniversityXiamen 361102 China

Although the efficacy of nucleos（t）ide analogue （NA） has been confirmed for treatment of chronic hepatitis B, long-term therapy has been recommended due to the high frequency of off-therapy viral DNA rebound and disease relapse. In this review, the RNA virion-like particles of hepatitis B virus （HBV） are integrated into the Hfe cycle of HBV replication, and the potential significance of serum HBV RNA is systematically described. The production of HBV RNA virion-like particles should not be blocked by NA; in this regard, serum HBV RNA is found to be a suitable surrogate marker for the activity of intrahepatic covalently closed circular DNA （cccDNA）, particularly among patients receiving NA therapy. Therefore, the concept of virological response is redefined as persistent loss of serum HBV DNA and HBV RNA. In contrast to hepatitis B surface antigen （HBsAg） that can originate from either the cccDNA or the integrated HBV DNA fragment, serum HBV RNA, with pregenomic RNA origination, can only be transcribed from cccDNA. Therefore, the loss of serum HBV RNA would likely be a promising predicator for safe drug discontinuation. The clinical status of consistent loss of serum HBV RNA accompanied with low serum HBsAg levels might be implicated as a ＂para-functional cure,＂ a status nearly close to the functional cure of chronic hepatitis B, to distinguish the ＂functional cure＂ characterized as serum HBsAg loss with or without anti-HBs seroconversion.

关键词： chronic hepatitis B serum HBV RNA nucleos(t)ide analogs virological response para-functional cure

Cloning and Function Identification of a Phytoene Desaturase Gene from Eucommia ulmoides

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Phyton-International Journal of Experimental Botany 2023年第5期92卷 1377-1389页

作者： Jiali Wang xiangmei chen Xiaozhen Huang Yichen Zhao Degang Zhao The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education) College of Tea SciencesCollege of Life SciencesGuizhou UniversityGuiyang550000China Plant Conservation Technology Center Guizhou Key Laboratory of Agricultural BiotechnologyGuizhou Academy of Agricultural SciencesGuiyang550000China

The phytoene desaturase(PDS)encodes a crucial enzyme in the carotenoid biosynthesis *** or inhibiting PDS expression leads to the appearance of mottled,chlorosis,or albino *** this study,the CDS sequence of EuPDS(Eucommia ulmoides Phytoene Desaturase)was first cloned and then PDS was silenced in Nicotiana *** showed the expression level of EuPDS in leaves was higher than that in the roots and *** *** leaves,which were treated by Agrobacterium for 24 h,photo-bleaching was shown on the fresh leaves one week after injection and the transcript level of PDS was down-regulated during the period of *** suggested that EuPDS could silence PDS of ***,so as to cause the phenotype of leaf *** is the main reporter gene involved in virus-induced gene silencing(VIGS).This study offered molecular evidence for identifying PDS gene involved in Carotenoid’s biosynthesis pathway and the regulation networks in *** also laid a useful foundation for study on leaf discoloration mechanism of other woody plants.

关键词： Eucommia ulmoides EuPDS virus-induced gene silencing TRV relative expression

Immune Responses to Six Synthetic Peptides of Capsid Protein with Sera from HIV-1 Infected Individuals

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Cellular & Molecular Immunology 2005年第4期2卷 289-293页

作者： Guangjie Liu Liumeng Yang Jianhua Wang Gaohong Zhang xiangmei chen Yongtang Zheng Laboratory of Molecular Immunopharmacology Kunming Institute of Zoology Chinese Academy of Sciences Kunming Yunnan 650223 China The Graduate School of the Chinese Academy of Sciences Beijing 100039 China

Many B cell epitopes within p24 of human immunodeficiency virus type 1 （HIV-1） were identified, while most of them were determined by using murine monoclonal antibodies reacting with overlapping peptides of p24. Therefore these epitopes may not represent the actual epitopes recognized by the HIV-1 infected individuals. In the present study, immune responses of 67 HIV-1 positive sera from Yunnan Province, China to five peptides on p24 of HIV-1 and one of HIV-2 were analyzed. All of 67 sera did not recognize peptide GA-12 on HIV-1 and peptide AG-23 on HIV-2, which indicated that GA-12 was not human B cell epitope and AG-23 did not cross-react with HIV-1 positive serum. Except 13 sera （19.4%）, all remaining sera did not recognize peptides NI-15, DR-16, DC-22 and PS-18, which indicated that these four peptides represented B cell linear epitopes of HIV-1 p24 in some HIV-1 infected individuals but not the immuno-dominant epitopes in most individuals.

关键词： HIV-1 human serum immune response p24 synthetic peptide

Expression of EGFP/SDCT1 fusion protein,subcellular localization signal analysis,tissue distribution and electrophysiological function study

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Science China(Life Sciences) 2004年第6期47卷 530-539页

作者： BAI Xueyuan chen xiangmei FEN Zhe WU Di HOU Kai chenG Genyang PENG Lixia Chinese PLA Kidney Center & Key Laboratory for Nephrology Chinese PLA General Hospital & Military Medical Postgraduate CollegeBeijing 100853China

Full-length cDNA gene of sodium-dependent dicarboxylate co-transporter protein 1 (SDCT1) is cloned from normal human kidney tissue and inserted into EGFP (enhanced green fluorescent protein) expression vector along with N-terminal and C-terminal truncated SDCT1 genes, so to construct the eukaryotic expression vectors of EGFP/SDCT1 fusion proteins, which are transfected into human renal tubular epithelial cells (HKC). Subcellular localizations of these fusion proteins are observed by laser confocal microscope to determine the localization signal of the SDCT1 protein. Duplex PCR analysis validates that the fusion protein genes have been in- tegrated into the genome of HKC. Western blot indicates that the fusion proteins have been ex- pressed in HKC. Confocal microscopy analysis shows that human SDCT1 predominantly locates on the plasma membrane, which is consistent with the results predicted by bioinformatics ap- proach; in HKC transfected with N-terminal truncated SDCT1 gene, the green fluorescence is mainly distributed on the plasma membrane; in HKC transfected with C-terminal truncated SDCT1 gene, the green fluorescence is mainly distributed in the cytoplasm. EGFP/SDCT1 mRNAs obtained by in vitro transcription are microinjected into Xenopus laevis oocytes for ex- pression and the trans-membrane currents are measured by using two-microelectrode volt- age-clamp technique. Na+ inward currents are present on cellular membrane of the injected oo- cytes. Immunohistochemical staining shows that human SDCT1 proteins are expressed on lu- men membrane of the renal proximal tubule, but are negative in distal tubule, collecting duct, renal interstitium and glomerulus. The above-mentioned studies suggest that human SDCT1 protein is located on the lumen membrane of the renal proximal tubule, the C-terminal sequence of the SDCT1 is required for delivery and targeting localization, and the plasma membrane lo- calization signal of the SDCT1 protein maybe locate in the C-terminal se

关键词： dicarboxylate co-transporter tricarboxylic acid cycle renal tubule subcellular localization.

An in-depth analysis of proteomics expression profiling in rat glomeruli utilizing LC-MS

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Chinese Science Bulletin 2010年第20期55卷 2142-2151页

作者： HONG Quan XUE Peng LU Yang chen xiangmei QI Ka WU Di Kidney Department & Institute of Nephrology Division of Clinical Internal Medicine Chinese PLA General Hospital Beijing 100853 China Laboratory of Proteomics Institute of Biophysics Chinese Academy of Sciences Beijing 100101 China

Glomeruli are an essential functional element of renal *** majority of renal diseases caused by glomerular sclerosis or fibrosis may result in renal dysfunction.A fomulate protein profile,a comprehensive analysis of glomeruli of normal rats was conducted in this study via protein *** annotation and classification of these proteins were performed and it was found that 26 had the same glomerule(endothelial cells,podocytes and mesangial cells)markers with proteins.

关键词：蛋白质组学肾小球大鼠 MS法 LC 作者内皮细胞肾功能不全

Cell Cycle Arrest Protein CDKN2C Is Not an HBV Host Factor

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Virologica Sinica 2021年第4期36卷 810-813页

作者： Guiwen Guan Liwei Zheng Jingyuan Xi Xingwen Yang xiangmei chen Fengmin Lu Peking University People's Hospital Peking University Hepatology InstituteBeijing Key Laboratory of Hepatitis C and Immunotherapy for Liver DiseasesBeijing 100044China Department of Microbiology&Infectious Disease Center School of Basic Medical SciencesPeking University Health Science CenterBeijing 100191China

Dear Editor,Hepatitis B virus(HBV)infection remains a major cause of liver diseases worldwide,which affects approximately 250 million people globally(Yuen et al.2018).Individuals with chronic HBV infection are at high risk of developing liver cirrhosis,and ultimately hepatocellular carcinoma(HCC).HBV is a Hepadnavirus DNA virus,which specifically infects and efficiently replicates in *** has been proposed for decades that HBV replicates preferentially in non-dividing quiescent hepatocytes(Ozer et al.1996).

关键词： al. infection diseases

Sugemule-10:Source of prescription and modern clinical application

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Chinese Herbal Medicines 2022年第3期14卷 376-384页

作者： xiangmei chen Liangliang Hadanbaoligao Zhang Buriebao Xiulan Wang Wurihan Tai Affiliated Hospital of Inner Mongolia Minzu University Tongliao 028005China NMPA Key Laboratory for Quality Control of Traditional Chinese Medicine(Mongolian Medicine) Tongliao 028000China National and Local Joint Engineering Research Center for Mongolian Medicine Research and Development(Development and Reform Commission) Tongliao 028000China Key Laboratory of Mongolian Medicine R&D Engineering Ministry of EducationTongliao 028000China Mongolian Medicine Research and Health Science Center of Inner Mongolia Minzu University Tongliao 028000China

Sugemule-10,one of the traditional Mongolian medicine(TMM)formulae,is derived from Four Medical Classics(Vol.4)and composed of 10 Mongolian *** is used to treat kidney cold,low back pain,urinary obstruction,kidney/bladder stones,and is the main prescription for kidney *** current research on Sugemule-10 is mostly focused on its clinical efficacy,and few papers are available upon its historical ***,we systematically reviewed Sugemule-10 from the aspects of prescription source,prescription interpretation,efficacy evolution,and modern clinical applications.

关键词： clinical application prescription interpretation prescription source Sugemule-10

Risk factors of prognosis after acute kidney injury in hospitalized patients

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Frontiers of Medicine 2017年第3期11卷 393-402页

作者： Sasa Nie Zhe Feng Lihua Xia Jiuxu Bai Fenglin Xiao Jian Liu Li Tang xiangmei chen Department of Nephrology Chinese PLA General Hospital Chinese PLA Institute of Nephrology State Key Laboratory of Kidney DiseasesNational Clinical Research Center for Kidney Diseases Beijing Key Laboratory of Kidney Diseases Beijing 100853 China

The risk factors, especially laboratory indicators, of prognosis after acute kidney injury （AK1） remain unclear. We conducted a retrospective survey of Chinese People＇s Liberation Army General Hospital from January 1, 2012 to December 31, 2012 according to the AKI diagnosis standard issued by Kidney Disease Improving Global Outcomes. The epidemiological features and factors influencing hospital mortality and renal function recovery were evaluated through logistic regression analysis. Among 77 662 cases of hospitalized patients, 1387 suffered from AKI. The incidence rate and mortality of AKI were 1.79~ and 14.56%, respectively. Multivariate logistic regression analysis revealed that high AKI stage, age greater than 80 years, neoplastic disease, low cardiac output, increased white blood cell count, and decreased platelet count and serum albumin levels were the risk factors affecting the mortality of AKI patients. Conversely, body mass index between 28 and 34.9 was a protective factor. Increased AKI stage, tumor disease, post-cardiopulmonary resuscitation, and RRT were the risk factors of renal function recovery upon discharge. In addition to traditional risk factors, white blood cell count, platelet count, albumin, and BMI were the predictors of the mortality of AKI patients. No laboratory indicators were found to be the risk factors of renal function recovery in AKI patients.

关键词： acute kidney injury risk factors prognosis