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Comparative Transcriptome Profiling of Glycine soja Roots Under Salinity and Alkalinity Stresses Using RNA-seq

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Journal of Northeast Agricultural University(English Edition) 2018年第3期25卷 29-43页

作者： Zhu Yan-ming Li Ji-na DuanMu Hui-zi Yin kui-de cheng Shu-fei chen Chao Cao Lei Duan Xiang-bo chen ran-ran College of Life Sciences Northeast Agricultural University College of Life Sciences Heilongjiang University College of Life Sciences and Technology Heilongjiang Bayi Agricultural University

Saline-alkaline stress can dramatically inhibit plant growth and limit crop production. Wild soybean （Glycine soja） is a crop that adapts well to such environmental stresses. In this study, RNA-sequencing technology was used to analyze the transcriptome profles of G. soja roots subjected to 50 mmol·L^-1 NaHCO3 and 150 mmol·L^-1 NaCl treatments. Totally, 2 125 differentially-expressed genes （DEGs） after NaCl treatment and 1 839 DEGs after NaHCO3 treatment were identifed. The top 14 DEGs revealed by RNA-seq were analyzed using qRT-PCR （quantitative real-time polymerase chain reaction）. Gene ontology （GO） annotation showed that most of DEGs under salt and alkali stresses were enriched in ＂metabolic process＂, ＂catalytic activity＂ and ＂binding＂ terms. To search for transcription factors （TFs） among DEGs, the data were screened against TF database PlantTFDB, and it was found that fve TF families, Apetala2/ethylene-responsive element binding proteins （AP2-EREBP）, V-myb avian myeloblastosis viral oncogene homolog （MYB）, WRKYGQK and Zinc fnger motif （WRKY）, NAM, ATAF1/2, CUC1/2 （NAC） and Cys2/His2 （C2H2） were involved in salt stress response. Other fve TF families, NAC, WRKY, MYB, AP2-EREBP and bZIP were involved in response to alkali stress. These two stress treatments shared NAC, WRKY, AP2-EREBP and MYB, and the only two different TFs were bZIP and C2H2. Forty-eight MYB TFs were differentially expressed under salt and alkali stresses, and most of them were up-regulated. This study provided useful information for further investigation of DEGs and TFs in response to saline and alkaline stresses and helped in understanding the molecular basis of the response of G. soja to saline and alkaline stresses.

关键词： RNA-seq wild soybean salt stress alkali stress

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建筑业发展阶段划分

土木工程与管理学报 2017年第3期34卷 57-61,90页

作者：郑磊陈光冲冉然东南大学土木工程学院江苏南京210096

建筑业作为国民经济的重要物质生产部门,对国民经济发展至关重要。以世界先进建筑业的发展特征即工业化、绿色化、信息化为主要研究对象,借鉴工业发展历程的划分方法,按照革命性技术、理念、标准的产生将建筑业"三化"的发展划分为不同... 详细信息

建筑业作为国民经济的重要物质生产部门,对国民经济发展至关重要。以世界先进建筑业的发展特征即工业化、绿色化、信息化为主要研究对象,借鉴工业发展历程的划分方法,按照革命性技术、理念、标准的产生将建筑业"三化"的发展划分为不同的阶段,利用时间轴将建筑业"三化"集成融合,形成建筑业1.0、建筑业2.0、建筑业2.5、建筑业3.0、建筑业4.0五个阶段。根据建筑业发展阶段划分标准,结合江苏建筑业发展现状,得出江苏建筑业处于建筑业2.5向3.0的发展阶段。

关键词：建筑业发展阶段工业化绿色化信息化

Salsolinol as an RNA m~6A methylation inducer mediates dopaminergic neuronal death by regulating YAP1 and autophagy

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Neural Regeneration Research 2025年第3期20卷 887-899页

作者： Jianan Wang Yuanyuan ran Zihan Li Tianyuan Zhao Fangfang Zhang Juan Wang Zongjian Liu Xuechai chen Beijing International Science and Technology Cooperation Base for Antiviral Drugs College of Chemistry and LifeBeijing University of TechnologyBeijingChina Department of Rehabilitation Beijing Rehabilitation HospitalCapital Medical UniversityBeijingChina

Salsolinol(1-methyl-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline,Sal)is a catechol isoquinoline that causes neurotoxicity and shares structural similarity with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine,an environmental toxin that causes Parkinson's ***,the mechanism by which Sal mediates dopaminergic neuronal death remains *** this study,we found that Sal significantly enhanced the global level of N~6-methyladenosine(m~6A)RNA methylation in PC12 cells,mainly by inducing the downregulation of the expression of m~6A demethylases fat mass and obesity-associated protein(FTO)and alk B homolog 5(ALKBH5).RNA sequencing analysis showed that Sal downregulated the Hippo signaling *** m~6A reader YTH domain-containing family protein 2(YTHDF2)promoted the degradation of m~6A-containing Yes-associated protein 1(YAP1)mRNA,which is a downstream key effector in the Hippo signaling ***,downregulation of YAP1 promoted autophagy,indicating that the mutual regulation between YAP1 and autophagy can lead to *** findings reveal the role of Sal on m~6A RNA methylation and suggest that Sal may act as an RNA methylation inducer mediating dopaminergic neuronal death through YAP1 and *** results provide greater insights into the neurotoxic effects of catechol isoquinolines compared with other studies and may be a reference for assessing the involvement of RNA methylation in the pathogenesis of Parkinson's disease.

关键词： ALKBH5 autophagy FTO Hippo pathway m~6A Parkinson's disease RNA methylation salsolinol YAP1 YTHDF2

基于VFM的污水处理项目PPP模式适用性研究

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中国农村水利水电 2017年第1期 49-53,57页

作者：陈然然丰景春薛松河海大学商学院南京211100 河海大学项目管理研究所南京211100 江苏省"世界水谷"与水生态文明协同创新中心南京211100 河海大学国际河流研究中心南京211100

污水处理是城市基础设施建设的重要内容,近年来,我国污水处理行业处于快速成长期,对污水处理项目的建设无论是从技术上还是资金支持上都带来一定程度的压力。PPP模式在我国已得到初步推广使用,将PPP模式引入污水处理项目,可以带来诸多... 详细信息

污水处理是城市基础设施建设的重要内容,近年来,我国污水处理行业处于快速成长期,对污水处理项目的建设无论是从技术上还是资金支持上都带来一定程度的压力。PPP模式在我国已得到初步推广使用,将PPP模式引入污水处理项目,可以带来诸多好处。然而,并非所有的项目都适用PPP模式,政府部门在进行PPP项目决策时需要科学的方法与依据。将物有所值方法应用到PPP模式适用性的评估中,结合我国污水处理行业的特点,分析物有所值定性和定量评价方法,为PPP模式的推广提供科学依据。

关键词：污水处理 PPP 物有所值适用性

NOX4 exacerbates Parkinson's disease pathology by promoting neuronal ferroptosis and neuroinflammation

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Neural Regeneration Research 2025年第7期20卷 2038-2052页

作者： Zhihao Lin Changzhou Ying Xiaoli Si Naijia Xue Yi Liu ran Zheng Ying chen Jiali Pu Baorong Zhang Department of Neurology The Second Affiliated HospitalZhejiang University School of MedicineHangzhouZhejiang ProvinceChina

Parkinson's disease is primarily caused by the loss of dopaminergic neurons in the substantia nigra ***,a novel form of regulated cell death characterized by iron accumulation and lipid peroxidation,plays a vital role in the death of dopaminergic ***,the molecular mechanisms underlying ferroptosis in dopaminergic neurons have not yet been completely *** oxidase 4 is related to oxidative stress,however,whether it regulates dopaminergic neuronal ferroptosis remains *** aim of this study was to determine whether NADPH oxidase 4 is involved in dopaminergic neuronal ferroptosis,and if so,by what *** found that the transcriptional regulator activating transcription factor 3 increased NADPH oxidase 4 expression in dopaminergic neurons and astrocytes in an 1-methyl-4-phenyl-1,2,3,6 tetrahydropyridine-induced Parkinson's disease *** oxidase 4 inhibition improved the behavioral impairments observed in the Parkinson's disease model animals and reduced the death of dopaminergic ***,NADPH oxidase 4 inhibition reduced lipid peroxidation and iron accumulation in the substantia nigra of the Parkinson's disease model ***,we found that NADPH oxidase 4 interacted with activated protein kinase Cαto prevent ferroptosis of dopaminergic ***,by lowering the astrocytic lipocalin-2 expression,NADPH oxidase 4 inhibition reduced 1-methyl-4-phenyl-1,2,3,6 tetrahydropyridine-induced *** findings demonstrate that NADPH oxidase 4 promotes ferroptosis of dopaminergic neurons and neuroinflammation,which contribute to dopaminergic neuron death,suggesting that NADPH oxidase 4 is a possible therapeutic target for Parkinson's disease.

关键词： dopaminergic neuron ferroptosis NADPH oxidase 4(NOX4) neuroinflammation Parkinson's disease

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环状RNA与疾病关系的研究进展

基础医学与临床 2016年第9期36卷 1295-1300页

作者：陈冉冉付玉荣伊正君潍坊医学院医学检验学系山东潍坊261031

环状RNA是最近发现的一类内源性非编码RNA,由内含子配对驱动的环化等模型产生,受相关调控因子调控。环状RNA的一个重要生物学功能是作为miRNA海绵,使miRNA水平在短时间内发生明显改变。环状RNA与疾病密切联系,是具有诊断和治疗潜力的分... 详细信息

环状RNA是最近发现的一类内源性非编码RNA,由内含子配对驱动的环化等模型产生,受相关调控因子调控。环状RNA的一个重要生物学功能是作为miRNA海绵,使miRNA水平在短时间内发生明显改变。环状RNA与疾病密切联系,是具有诊断和治疗潜力的分子。本文论述环状RNA的生物合成及调控、环状RNA的生物学功能以及环状RNA与疾病的关系。

关键词：环状RNA 非编码RNA 内含子配对驱动的环化 miRNA海绵

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影响我国税收收入经济因素的实证分析

西南交通大学学报（社会科学版） 2011年第3期12卷 64-66页

作者：陈然然西南交通大学公共管理学院四川成都610031

税收收入是我国财政收入的基础,现有研究成果表明,有很多经济性因素都影响着税收收入。通过对包括国内生产总值、财政支出、商品零售价格指数等五个指标影响税收收入的回归模型分析,可得出以下结论:影响我国税收收入的主要因素是国内生... 详细信息

税收收入是我国财政收入的基础,现有研究成果表明,有很多经济性因素都影响着税收收入。通过对包括国内生产总值、财政支出、商品零售价格指数等五个指标影响税收收入的回归模型分析,可得出以下结论:影响我国税收收入的主要因素是国内生产总值、财政支出和商品零售价格指数;而各因素数量的变化引起税收总量变化的程度由各自的系数来反映。

关键词：税收收入国内生产总值财政支出商品零售价格指数进出口总额固定资产投资