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Chemokine-like factor 1 (CKLF1) is expressed in myocardial ischemia injury in vivo and in vitro

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BIOCELL 2024年第6期48卷 981-990页

作者： JULING FENG HAODONG chen YANGBO LIU QIDI AI YANTAO YANG LEI ZHAO SHIFENG CHU naihong chen School of Pharmacy Department of PharmacyHunan Engineering Technology Center of Standardization and Function of Chinese Herbal Decoction PiecesHunan University of Chinese MedicineChangsha410208China Department of Pharmacology State Key Laboratory of Bioactive Substances and Functions of Natural MedicinesInstitute of Materia Medica&Neuroscience CenterChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing100050China Department of Diagnostics Research Lab of Translational Medicine Hengyang Medical CollegeUniversity of South ChinaHengyang421001China Department of Gastrointestinal Surgery The First Affiliated HospitalHengyang Medical CollegeUniversity of South ChinaHengyang421001China

Introduction:Chemokine-like factor 1(CKLF1)is a chemokine that is overexpressed in several *** previousﬁndings revealed a signiﬁcant increase in CKLF1 expression in the ischemic brain,suggesting its potential as a therapeutic target for ischemic ***:In this study,we examined the expression dynamics of CKLF1 in both in vivo and in vitro models of ischemic cardiac *** infarction(MI)was induced in vivo by ligation of the left anterior descending artery(LAD)of the rat *** levels of CKLF1,Creatine Kinase MB Isoenzyme(CK-MB),and Lactate dehydrogenase(LDH)in the serum were detected using Enzyme-linked immunosorbent assay(ELISA).The expression of CKLF1 in the infarcted area was detected by immunohistochemistry,immunoﬂuorescence,quantitative PCR(qPCR),and Western blotting(WB).H9C2 and AC16 cardiomyocytes cultured in vitro were subjected to oxygen and glucose deprivation(OGD).LDH was used to detect cell damage,and CKLF1 expression was detected by qPCR and ***:CKLF1 mRNA and protein expression were signiﬁcantly increased in h9c2 cells at 1.5 h and in AC16 cells at 4 h after *** serum CK-MB in rats increased signiﬁcantly on theﬁrst day after infarction,while the LDH concentration increased signiﬁcantly on the third day after ***1 blood levels signiﬁcantly increased on theﬁrst day following MI in ***1 expression notably increased in the infarct area on days 1,3,and 7 *** MI tissue,CKLF1 colocalizes with cardiomyocytes,macrophages,and ***:CKLF1 was substantially expressed during myocardial ischemia injury both in vivo and in vitro and was colocalized with macrophages and neutrophils,indicating that CKLF1 is expected to be a biomarker and a drug target for the treatment of myocardial infarction.

关键词： Chemokine-like factor 1 Overexpression Myocardial infarction

Protein kinase-based neural signaling pathways for ginsenosides:a retrospective review

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Journal of Traditional Chinese Medicine 2015年第3期35卷 349-354页

作者： He Wenbin Zhang Junlong chen naihong Department of Basic Medicine Shanxi University of Traditional Chinese Medicine(Shanxi Key Laboratory of Chinese Medicine EncephalopathyHigher Education Key Laboratory of Brain Science in Shanxi Province) Department of Pharmacology Institute of Materia MedicaChinese Academy of Medical Sciences & Peking Union Medical College(Key Laboratory of Bioactive Substances and Resources UtilizationMinistry of Education)

Ginsenosides are the main active components of ginseng,which have been reported to target brain tissues and produce multiple neuroprotective *** have been shown to improve learning ability and memory in normal aged animals,and in an animal model of memory ***,its underlying pharmacological mechanisms are very complicated,especially with regard to its effects on the activation of protein kinases in *** reports have shown that some protein kinases may be affected by ginsenosides,including protein kinase C,calcium/calmodulin-dependent protein kinase Ⅱ,c-Jun-N terminal kinase,and protein tyrosine *** this paper,protein kinases that may underlie the mechanisms of ginsenosides will be discussed.

关键词：蛋白激酶C 神经保护作用人参皂甙传导通路蛋白酪氨酸激酶动物模型钙调蛋白蛋白激酶Ⅱ

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抑郁症发病机制:聚焦蛋白水平与基因异常

中华神经科杂志 2023年第9期56卷 1078-1083页

作者：王惠芹陈乃宏湖南中医药大学药学院湖南省中药饮片标准化及功能工程技术研究中心长沙410208 中国医学科学院药物研究所神经科学中心北京100050

抑郁症是常见的精神类疾病,临床表现为情绪低落、精神运动迟缓、动力减弱、快感缺失、绝望甚至自杀。抑郁症患者临床表现的异质化表明其发病机制具有多样性。蛋白水平及基因异常是抑郁症发病的一个重要因素。文中聚焦抑郁症相关蛋白的... 详细信息

抑郁症是常见的精神类疾病,临床表现为情绪低落、精神运动迟缓、动力减弱、快感缺失、绝望甚至自杀。抑郁症患者临床表现的异质化表明其发病机制具有多样性。蛋白水平及基因异常是抑郁症发病的一个重要因素。文中聚焦抑郁症相关蛋白的水平及其调控基因,进一步阐述抑郁症的发病机制,并提出目前抑郁症研究领域存在的问题和挑战,为开发新型抗抑郁药物提供理论基础。

关键词：抑郁症蛋白质基因发病机制

Helioscopianoids A–Q,bioactive jatrophane diterpenoid esters from Euphorbia helioscopia

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Acta Pharmaceutica Sinica B 2018年第5期8卷 805-817页

作者： Zhenpeng Mai Gang Ni Yanfei Liu Zhao Zhang Li Li naihong chen Dequan Yu State Key Laboratory of Bioactive Substance and Function of Natural Medicines Institute of Materia Medica Chinese Academy of Medical Sciences and Peking Union Medical College

The EtOH extracts of the whole plants of Euphorbia helioscopia afforded 17 new jatrophane diterpenoid esters, helioscopianoids A–Q(1–17), along with eight known compounds(18–25). Their structures were elucidated by extensive spectroscopic methods and Mo_2(OAc)_4-induced ECD analysis,and the structures of compounds 1, 2, and 7 were confirmed by X-ray crystallography. Compounds 1–17 were evaluated for inhibitory effects on P-glycoprotein(P-gp) in an adriamycin(ADM)-resistant human breast adenocarcinoma cell line(MCF-7/ADR) and neuroprotective effects against serum deprivationinduced and rotenone-induced PC12 cell damage. Compounds 8 and 16 increased the accumulation of ADM in MCF-7/ADR cells by approximately 3-fold at a concentration of 20 μmol/L. Compound 8 could attenuate rotenone-induced PC12 cell damage, and compounds 2, 8, and 12 showed neuroprotective activities against serum deprivation-induced PC12 cell damage.

关键词： Euphorbia helioscopia Jatrophanes Helioscopianoids A–Q P-glycoprotein(ABCB1) inhibitory activity Neuroprotective effect

Efficacy of Lidan Tang on high-fat-diet induced gallstone in mice and possible mechanism

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Journal of Traditional Chinese Medicine 2020年第4期40卷 584-592页

作者： Gao Yan Liu Caipin Li Juntong Zhai Yingying Lin Meiyu Wu Qinglin Chu Shifeng Zhang Zhao Li Jianping Zhou Xin Li Yueting chen naihong State Key Lab of Bioactive Substances and Functions of Natural Medicines Institute of Materia MedicaNeuroscience CenterChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100050China Department of Pharmacy Xuanwu Hospital of Capital Medical UniversityBeijing 100053China Department of Surgery Beijing Hospital of Integrated Traditional and Western MedicineBeijing 100039China Institute of Clinical Pharmacology Guangzhou University of Chinese MedicineGuangzhou 510405China College of Pharmacy Hunan University of Chinese MedicineChangsha 410208China

OBJECTIVE:To investigate efficacy of Lidan Tang(LDT)on gallstone induced by high fat diet in mice,and to study its underlying ***:Mice were fed with high fat diet every day and treated with LDT(9.01 times of human clinic dosage).Mice were randomly divided into 6 groups as control group,gallstone model group(high-fat diet),positive control ursodeoxycholic acid(UDCA)group(80 mg·kg^-1·d^-1,i.g.),LDT low dose group(6 kg/d,i.g.),LDT middle dose group(12 kg/d,i.g.),and LDT high dose group(24 kg/d,i.g.).The whole experiment was lasted for 4 *** levels of ALT,AST,LDH,CHO,HDL-C and LDL-C in serum were measured,the pathological sections were observed by hematoxylin-eosin staining,the activities of antioxidant enzymes were measured by kits,and the proteins related to oxidative stress and lipid transport were detected by Western blot ***:LDT could significantly reduce the contents of ALT and AST in serum and improve the pathological tissue of *** could significantly reduce the content of MDA and LPO,and increase the level of GSH and GSH-PX in liver *** data of Western blot showed that LDT had antioxidant effect promoting Keap1/Nrf2 pathway and regulated the process of lipid transport,which was statistically *** addition,LDT treatment inhibited the expression of ATP-binding cassette transports ABCG5/8 in liver,and reduced cholesterol transport from the hepatocytes to the ***:LDT has protective effect on gallstones induced by high fat diet in mice,which might be based on the protective effect on liver,including enhancing the antioxidant capacity of liver and reducing the production of lipid peroxides.

关键词： Cholelithiasis ATP-binding cassette transporters Oxidative stress Lipids Lidan Tang

Insight into Medicinal Chemistry Behind Traditional Chinese Medicines: p-Hydroxybenzyl Alcohol-Derived Dimers and Trimers from Gastrodia elata

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Natural Products and Bioprospecting 2021年第1期11卷 31-50页

作者： Yanan Wang Min Zhang Xue Zhou chengbo Xu chenggen Zhu Yuhe Yuan naihong chen Yongchun Yang Qinglan Guo Jiangong Shi State Key Laboratory of Bioactive Substance and Function of Natural Medicines Institute of Materia MedicaChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100050China

From an aqueous extract of“tian ma”(the steamed and dried rhizomes of Gastrodia elata),ten new compounds gastrodiben-zins A−D(1−4)and gastrotribenzins A−F(5−10),along with known analogues(11−20),having structure features coupling between two and three p-hydroxybenzyl-derived units via carbon-and/or ether-bonds,were isolated and characterized by spectroscopic data ***,the new compounds 5a,6a,8a,22,and 23,as well as the known derivatives 13a,14a,15,17−21,24,25,and p-hydroxybenzyl aldehyde were isolated and identified from a refluxed aqueous solution of p-hydroxybenzyl *** of 5a and 6a in methanol and ethylation of 6a,8a,13a,and 14a in ethanol produced 5 and 6 and 7,8,13,and 14,*** ultra-performance liquid chromatography high-resolution electrospray ioniza-tion mass spectrometry(UPLC-HRESIMS)analysis of the refluxed solutions of p-hydroxybenzyl alcohol and the refluxed extracts of the fresh *** rhizome and“tian ma”extracts indicated consistent production and variation of the dimeric and trimeric derivatives of p-hydroxybenzyl alcohol upon extracting solvents and refluxing *** various assays,the dimeric and trimeric derivatives showed more potent activities than p-hydroxybenzyl alcohol itself and gastrodin,which are the main known active constituents of“tian ma”.These results revealed for the first time that the more effective dimers and trimers can be produced through condensation of the co-occurring p-hydroxybenzyl alcohol during processing and decocting of the *** rhizomes,demonstrating insights into medicinal chemistry behind application protocols of traditional Chinese medicines.

关键词： Orchidaceae Gastrodia elata p-Hydroxybenzyl alcohol dimer p-Hydroxybenzyl alcohol trimer Gastrodibenzins Gastrotribenzins Medicinal chemistry behind Chinese medicines

Antidepressant-like effect of active fraction of Polyrhachisvicina Roger in a rat depression model

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Journal of Traditional Chinese Medicine 2018年第1期38卷 12-21页

作者： Wei Guining Chu Shifeng Su Qibiao Su Hua Lin Meiyu He Fei Lu Wenjie Lu Guoshou Huang Zhoufeng Tan Xiao Lin Xiao Zeng Xianbiao Wei Baowei chen naihong Department of Pharmacology Guangxi Institute of Chinese Medicine and Pharmaceutical ScienceNanning 530022China State Key Laboratory of Bioactive Substances and Functions of Natural Medicines Institute of Materia&Neuroscience CenterChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100050China College of Health Science Guangdong Pharmaceutical UniversityGuangzhou 510006China College of Pharmacy Hunan University of Chinese MedicineChangsha 410208China

OBJECTIVE: To investigate the antidepressant-likeeffect of active fraction of Polyrhachis vicina Roger(AFPR) in a rat depression model, and to elucidate the underlying ***: AFPR was extracted with ethanol followed by petroleum ether. Its antidepressant-like effect was investigated in mice by tail suspension test(TST), forced swimming test(FST) and open field test(OPT). A repeated dose of reserpine(0.5 mg/kg, daily for 14 d) was used to establish a rat depression model. Fluoxetine was used as positive control agent. The effect of AFPR on reserpine-induced ptosis, hypothermia and akinesia, the levels of monoamines and their metabolites, and the activity of monoamine oxidase(MAO) in hippocampus and prefrontal cortex were ***: Administration of AFPR by gavage at 160 and 320 mg/kg significantly reduced the duration of immobility in the FST and TST, and did not affect locomotor activity in the OPT. In the reserpine-induced depression model, AFPR attenuated anhedonia, demonstrated by reversing hypothermia, akinesia and sucrose consumption. AFPR significantly increased the concentration of monoamines, including dopamine, serotonin, noradrenaline and ***: AFPR normalized the metabolism rates of noradrenaline, serotonin and dopamine,and the activity of MAO, which were altered by chronic reserpine exposure. The findings suggest that modulation of the monoaminergic neurotransmitter system likely underlies the antidepressant-like effect of AFPR.

关键词：抗抑郁剂老鼠模型利血平持续时间调查结果低体温多巴胺

Systematic Review of Chinese Medicinal Formula for Depression in Parkinson＇s Disease

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中国药理通讯 2013年第3期30卷 44-45页

作者： Yi Zhang Zhenzhen Wang Hongmei Sun Ping Li Yunfeng Li naihong chen Department of Anatomy School of Preclinical Medicine Beijing University of Chinese Medicine Beijing 100029 China State Key Laboratory of Bioactive Substances and Functions of Natural Medicines Department of Pharmacology Institute of Materia Medica Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100050 China Department of Pharmacology Institute of Clinical Medical Sciences China Japan Friendship Hospital Beijing 100029 China Department of New Drug Evaluation Beijing Institute of Pharmacology and Toxicology Beijing 100850 China

Aim of the Study:Depression is the most common non-motor syndrome of Parkinson' s *** medicinal formula (CMF) has been used to treat depression for more than 2000 years. Recently, a few evidence-based reviews on CMF f... 详细信息

关键词：姜黄素中脑胶质细胞保护作用 CYP2E1表达活性

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高职院校在线开放课程质量评价研究

黑龙江科学 2022年第15期13卷 47-49页

作者：陈乃鸿景姗娜南京城市职业学院南京211200

为提升课程教学质量,论述了在线开放课程质量评价指标体系建设和在线开放课程质量评价方法,并基于在线开放课程建设现状,提出了在线开放课程建设策略:提升师资素养,完善内容架构,丰富功能负载,导引教师工作方向,动态监控学生学习过程,... 详细信息

为提升课程教学质量,论述了在线开放课程质量评价指标体系建设和在线开放课程质量评价方法,并基于在线开放课程建设现状,提出了在线开放课程建设策略:提升师资素养,完善内容架构,丰富功能负载,导引教师工作方向,动态监控学生学习过程,实现完善的评价反馈,更好地引导学生自主学习。

关键词：高职院校在线开放课程质量评价建设策略