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Evaluation of Genetic and Epigenetic Modification in Rapeseed(Brassica napus)Induced by Salt Stress

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Journal of Integrative Plant Biology 2007年第11期49卷 1599-1607页

作者： Guangyuan Lu Xiaoming Wu Biyun chen guizhen Gao Kun Xu Brassica Germplasm Division Oil Crops Research Institute Chinese Academy of Agricultural Science Wuhan 430062 China

Salinity is an important limiting environmental factor for rapeseed production worldwide. In this study, we assessed the extent and pattern of DNA damages caused by salt stress in rapeseed plants. Amplified fragment length polymorphism （AFLP） analysis revealed dose-related increases in sequence alterations in plantlets exposed to 10-1000 mmol/L sodium chloride. In addition, individual plantlets exposed to the same salt concentration showed different AFLP and selected region amplified polymorphism banding patterns. These observations suggested that DNA mutation in response to salt stress was random in the genome and the effect was dose-dependant. DNA methylation changes in response to salt stress were also evaluated by methylation sensitive amplified polymorphism （MSAP）. Three types of MSAP bands were recovered. Type Ⅰ bands were observed with both isoschizomers Hpa Ⅱ and Msp Ⅰ, while type Ⅱ and type Ⅲ bands were observed only with Hpa Ⅱ and Msp Ⅰ, respectively. Extensive changes in types of MSAP bands after NaCI treatments were observed, including appearance and disappearance of type Ⅰ, Ⅱ and Ⅲ bands, as well as exchanges between either type Ⅰand type Ⅱ or type Ⅰ and type Ⅲ bands. An increase of 0.2-17.6% cytosine methylated CCGG sites were detected in plantlets exposed to 10- 200 mmol/L salt compared to the control, and these changes included both de novo methylation and demethylation events. Nine methylation related fragments were also recovered and sequenced, and one sharing a high sequence homology with the ethylene responsive element binding factor was identified. These results demonstrated clear DNA genetic and epigenetic alterations in planUets as a response to salt stress, and these changes may suggest a mechanism for plants adaptation under salt stress.

关键词： Brassica napus DNA-methylation genetic variation methylation sensitive amplified polymorphism salt stress.

Brain neural effects of the ’tonifying kidney and benefiting marrow’ method in the treatment of osteoporosis

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Journal of Traditional Chinese Medicine 2019年第6期39卷 902-909页

作者： Xu Yunxiang Liu Xueqin Li Haolan Liu Hongyuan Pan Zhanxia chen guizhen Clinical Medical College of Acupuncture Moxibustion and RehabilitationGuangzhou University of Chinese MedicineGuangzhou 510006China Department of Rehabilitation Affiliated BaoAn Traditional Chinese Medicine HospitalGuangzhou University of Chinese MedicineShenzhen 518101China

In Traditional Chinese Medicine(TCM),the'tonifying kidney and benefiting marrow'(TKBM)method is based on the theory that kidney controls bones,while marrow moistens *** have been aboundant theoretical studies on kidney controlling bones since Huangdi's Internal ***-er,there are too few ones about the marrow moistening the *** generally use the tonifying kidney method instead of the TKBM method,which causes lack of a unified standard and theoretical basis in evaluating the TKBM ***,we first proposed the hypothesis that deficiency of marrow sea causes ***,we prove the scientific validity of this hypothesis from the side of the TCM theory on the relationship among kidney,bone and marrow sea physiologically and *** on this,we find that the TCM theory provides theoretical basis for deficiency of marrow sea causing *** the other side,Western Medicine theory holds that(a)physiologically,the brain regulates the bone mass via three pathways:the neuro-osteogenic network,neuro-endocrine-bone network,and neuropeptide-bone network;(b)pathologically,brain impacts bone mass via three major passways including the regulation of the sympathetic nervous system,secreting hormones that directly act on bone cells and regulating the synthesis and secretion of hormones in the intermediary organs,and neuropeptides such as neuropeptide Y(NPY),substance P(SP),and calcitonin gene-related peptide(CGRP).Evidences involving estrogen deficiency,sympatheticotonia,or neuropeptides imbalance prove that brain-bone mass regulation plays an important role in the pathogenesis of ***,we find that kidney invigoration method can change the concentrations of central neurotransmitters of norepinephrine and glutamate to regulate neuro-osteogenic network,and promote the recovery of ovarian function and have an estrogen-like effect by regulating the hypothalamus-pituitary-ovarian axis,which thus influences bone

关键词： Osteoporosis Brain(TCM) Reinforcing kidney Kidney controlling bones Brain-bone mass regulation

补肾固齿丸联合贝复新凝胶治疗肾阴虚型慢性牙周炎的临床效果

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中国医药导报 2024年第6期21卷 115-118,137页

作者：马穗齐周倩冰陈桂珍广东药科大学附属第一医院口腔科广东广州510000

目的探讨补肾固齿丸联合贝复新凝胶治疗肾阴虚型慢性牙周炎的临床效果。方法选取2021年9月至2022年12月广东药科大学附属第一医院收治的120例肾阴虚型慢性牙周炎患者,按随机数字表法将其分为对照1组、对照2组与研究组,每组40例。对照... 详细信息

目的探讨补肾固齿丸联合贝复新凝胶治疗肾阴虚型慢性牙周炎的临床效果。方法选取2021年9月至2022年12月广东药科大学附属第一医院收治的120例肾阴虚型慢性牙周炎患者,按随机数字表法将其分为对照1组、对照2组与研究组,每组40例。对照1组予以贝复新凝胶治疗,对照2组予以补肾固齿丸治疗,研究组给予贝复新凝胶联合补肾固齿丸治疗,三组均持续治疗12周。治疗结束后,评估三组临床疗效、症状好转时间及安全性,比较三组治疗前后牙周状况指标及炎症因子水平。结果研究组临床疗效优于对照1、2组,口腔异味、咀嚼疼痛、牙齿松动和牙龈水肿症状好转时间短于对照1、2组(P0.05)。结论补肾固齿丸联合贝复新凝胶治疗慢性牙周炎效果及安全性较高,有助于减轻患者炎症水平,改善其临床症状。

关键词：补肾固齿丸贝复新凝胶慢性牙周炎炎症因子

Detection of DNA methylation changes during seed germination in rapeseed (Brassica napus)

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Chinese Science Bulletin 2006年第2期51卷 182-190页

作者： LU Guangyuan WU Xiaoming chen Biyun GAO guizhen XU Kun LI Xiangzhi Key Laboratory of Oil Crops Genetic Improvement of the Ministry of Agriculture Oil Crops Research Institute CAAS Wuhan 430062 China

DNA methylation is known to play a crucial role in regulating plant development and or- gan or tissue differentiation. In this study, we as- sessed the extent and pattern of cytosine methylation during rapeseed (Brassica napus L.) seed germina- tion, and compared the methylation level of various tissues in seedling, using the techniques of methyla- tion-sensitive amplified polymorphism (MSAP) and HPLC separation and quantification of nucleosides. In all, 484 bands, each representing a recognition site cleaved by either or both of the isoschizomers, were amplified by 12 pairs of selective primers in DNA ob- tained from dry seeds. A total of 76 sites were found to be differentially digested by the isoschizomers, indicating that approximately 15.7% of 5′-CCGG-3′ sites in the genome were cytosine methylated. Four classes of patterns were observed in a comparative assay of cytosine methylation in the dry and germi- nating seeds; a small number of hypermethylation events occurred at 5′-CCGG-3′ sites in germinating seeds compared with dry seeds, while many more hypomethylation events were detected after seed germination. Differences in DNA methylation level in various tissues were also detected; radicel was less methylated than hypocotyl and cotyledon. These observations were further confirmed by HPLC analy- sis. In addition, sequencing of eleven differentially methylated fragments and the subsequent blast search revealed that cytosine methylated 5′-CCGG- 3′ sequences were equally distributed between cod- ing and non-coding regions. These results clearly demonstrate the power of MSAP technique for large-scale DNA methylation detection in rapeseed genome, and the complexity of DNA methylationchange during seed germination. DNA Hypomethyla- tion going with seed germination appears to be a necessary step toward transcriptional activation in gene expression, and may well contribute to the de- velopmental gene regulation.

关键词：油菜籽芸苔属 DNA甲基化作用种子萌芽 MSAP HPLC

分析医护一体化护理对病毒性肺炎合并呼吸衰竭患者睡眠质量的影响

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世界睡眠医学杂志 2024年第3期11卷 680-683页

作者：李雪婷陈慧莉张秀燕陈桂贞杨萍丽陈思如福建医科大学附属泉州第一医院泉州362000

目的:分析医护一体化护理对病毒性肺炎合并呼吸衰竭患者睡眠质量的影响。方法:选取2022年3月至2023年3月福建医科大学附属泉州第一医院收治的病毒性肺炎合并呼吸衰竭患者67例作为研究对象,按照随机数字表法分为对照组(n=33)和观察组(n=3... 详细信息

目的:分析医护一体化护理对病毒性肺炎合并呼吸衰竭患者睡眠质量的影响。方法:选取2022年3月至2023年3月福建医科大学附属泉州第一医院收治的病毒性肺炎合并呼吸衰竭患者67例作为研究对象,按照随机数字表法分为对照组(n=33)和观察组(n=34),2组均给予对症治疗,对照组给予常规护理模式干预,观察组给予医护一体化模式干预。采用匹兹堡睡眠质量指数(PSQI)比较2组患者睡眠质量的差异;在护理前1 d、护理10 d后测定患者的睡眠参数,应用睡眠监测仪测定睡眠时长、睡眠潜伏期、觉醒时间以及睡眠效率;在入院后1 d、护理10 d后通过肺功能检测仪测定患者的第1秒用力呼吸容积(FEV_(1))、用力肺活量(FVC)及第1秒用力呼吸容积和肺活量比值(FEV_(1)/FVC)。结果:干预后,2组患者的肺功能指标FEV_(1)、FVC及FEV_(1)/FVC均有改善,且观察组显著高于对照组,差异有统计学意义(P<0.05);观察组睡眠时长、睡眠效率显著高于对照组,觉醒时间显著短于对照组,差异有统计学意义(P<0.05);干预后,观察组PSQI评分显著低于对照组,差异有统计学意义(P<0.05)。结论:在病毒性肺炎合并呼吸衰竭患者临床治疗中配合医护一体化护理,可促进病情转归,改善患者睡眠质量。

关键词：医护一体化护理病毒性肺炎呼吸衰竭睡眠时长睡眠潜伏期觉醒时间睡眠效率

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河南省和河北省部分地区狐狸和貉十二指肠贾第虫和芽囊原虫感染情况分析

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中国兽医杂志 2024年第9期60卷 39-46页

作者：陈桂珍郭雨楠左守军眭玉珍卢营郑力萍宋鹏涛栗亮亮代宏宇张涛张龙现董海聚刘芳河南农业大学动物医学院河南郑州450046 北京农学院动物科学技术学院北京昌平102206

为了解狐狸和貉的十二指肠贾第虫和芽囊原虫感染情况以及相关影响因素,本试验从河南省(新乡和鹤壁)和河北省(秦皇岛)部分地区采集狐狸和貉的新鲜粪便样品共893份(其中狐狸312份、貉581份),提取粪便全基因组DNA,并分别基于贾第虫β-贾第... 详细信息

为了解狐狸和貉的十二指肠贾第虫和芽囊原虫感染情况以及相关影响因素,本试验从河南省(新乡和鹤壁)和河北省(秦皇岛)部分地区采集狐狸和貉的新鲜粪便样品共893份(其中狐狸312份、貉581份),提取粪便全基因组DNA,并分别基于贾第虫β-贾第虫素(BG)和芽囊原虫小亚基核糖体RNA(SSU rRNA)基因位点对粪便DNA样品进行PCR扩增,对阳性产物进行测序,经序列比对鉴定贾第虫和芽囊原虫基因亚型,构建系统进化树分析遗传进化关系。结果显示,狐狸和貉的十二指肠贾第虫和芽囊原虫总感染率均为1.79%(16/896);在来自新乡、鹤壁和秦皇岛的样品中,来自鹤壁的样品未检测出十二指肠贾第虫,来自新乡和秦皇岛的样品感染率分别为2.04%(11/540)和2.59%(5/193),3个地区均检测出芽囊原虫,感染率分别为1.48%(8/540)、1.25%(2/160)和3.11%(6/193);不同宿主间十二指肠贾第虫感染率存在显著差异(P<0.05);不同年龄段貉的芽囊原虫感染率存在显著差异(P<0.05);鉴定出的十二指肠贾第虫基因型主要为集聚体B~E;芽囊原虫基因型主要为ST1、ST2、ST3、ST5和ST7,且鉴定出的所有基因型均具有人兽共患性。本试验结果提示,狐狸和貉感染的十二指肠贾第虫和芽囊原虫存在人兽共患传播风险,具有重要的公共卫生意义。

关键词：狐狸貉十二指肠贾第虫芽囊原虫感染率

牙周炎患者唾液一氧化氮的含量及其与牙周病指标相关性的研究

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华西口腔医学杂志 1999年第2期17卷 140-142页

作者：陈桂珍孙伟莲浙江医科大学附属第二医院

目的：研究牙周炎患者唾液一氧化氮（ＮＯ）的含量，及其与牙周病各临床指标的相关性。方法：选择成人牙周炎患者２３例为实验组，健康人２７例为对照组。记录牙周炎患者的牙龈出血指数（ＧＢＩ）、牙周袋深度（ＰＤ）和附着丧失量（Ａ... 详细信息

目的：研究牙周炎患者唾液一氧化氮（ＮＯ）的含量，及其与牙周病各临床指标的相关性。方法：选择成人牙周炎患者２３例为实验组，健康人２７例为对照组。记录牙周炎患者的牙龈出血指数（ＧＢＩ）、牙周袋深度（ＰＤ）和附着丧失量（ＡＬ）。ＮＯ的含量由检测唾液亚硝酸盐的含量来代表。测定实验组和对照组所有病例的唾液ＮＯ的含量。结果：对照组唾液ＮＯ的含量为２８．８０６±６．６０４μｍ／Ｌ，男女间无显著性差异。实验组唾液ＮＯ的含量为５５．３６１±１３．３１９μｍｏｌ／Ｌ，明显高于对照组。牙周炎患者唾液ＮＯ的含量与ＰＤ、ＡＬ间存在明显的正相关。结论：牙周炎患者唾液ＮＯ的含量显著高于健康人，并与牙周炎的严重程度有关。

关键词：一氧化氮亚硝酸盐唾液牙周炎

Torsion-Mirror Optical Actuators with Compound Driving Structures

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Torsion-Mirror Optical Actuators with Compound Driving Struc...

DESIGN OF A DISPLACEMENT/ANGLE MEASUREMENT SYSTEM BASED ON LASER TRIANGULATION PRINCIPLE

Photonics Asia 2004;Optoelectronics,Microelectronics,and Nanotech

作者： Wengang Wu*,Qinghua chen,guizhen Yan,Yilong Hao Institute of Microelectronics,Peking University,Beijing 100871,China

A new kind of torsion-mirror optical actuators with compound driving structures is reported. The advanced compound driving structures are made up of plate-electrode couple drives and vertical comb dri

关键词： MEMS,torsion-mirror actuator,compound driving,mixed silicon micromachining,optical coupling loss

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DESIGN OF A DISPLACEMENT/ANGLE MEASUREMENT SYSTEM BASED ON L...