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YAP is oppositely regulated in i PSC-induced cardiovascular progenitor cell and vascular smooth muscle cell differentiation

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中国病理生理杂志 2016年第8期32卷 1532-1532页

作者： WANG Yong-yu FAN Xiao-fang DING Lu chen dan-yang ZHAO Ru LI Lan GONG Yong-sheng Institute of Hypoxia Medicine Wenzhou Medical University

AIM: To explore whether YAP protein is important in induced pluripotent stem cell( i PSC)-induced cardiovascular progenitor cell and / or vascular smooth muscle differentiation. METHODS: Using episomal vector based reprogramming,we generated human i PSCs from donor fibroblasts. We used both this i PSCs and human H1 embryonic stem cells to differentiate into vascular smooth muscle cells(VSMCs) through cardiovascular progenitor cells(CVPC). Western blotting,q PCR and immunofluorescence microscopy were used to check the expression of YAP and related genes during this differentiation process. RESULTS: The results showed that i PSCs expressed pluripotent stem cell markers,such as Oct4,Nanog,Sox2,TRA-1-60 and SSEA3,and could form teratoma in SCID mice. YAP was highly expressed in pluripotent stem cells,but dramatically decreased when CVPC differentiation started. YAP gradually increased during CVPC three-day differentiation. The TAZ and YAP binding partner TEAD1,but not TEAD2 and TEAD4,have similar expression pattern in CVPC differentiation. Immunofluorescence result confirmed that YAP was activated and accumulated in nucleus. Interestingly,both YAP and phosphorylated YAP expression decreased to very low level after CVPC differentiated into VSMCs in 7 days. TEAD4 and TAZ also decreased,while TEAD1,TEAD2 and TEAD3 expression did not change during VSMC differentiation. CONCLUSION: YAP and TEAD1 expression increased during CVPC differentiation,while YAP and TEAD4 expression decreased from CVPC to VSMCs differentiation,which suggested YAP might have different function during diverse cell differentiation.

关键词： YAP is oppositely regulated in i PSC-induced cardiovascular progenitor cell and vascular smooth muscle cell differentiation cell PSCs

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靶向p21蛋白激活激酶2逆转非小细胞肺癌细胞吉非替尼耐药

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中国生物化学与分子生物学报 2021年第8期37卷 1078-1084页

作者：黄渊锋李盼杨明强刘浩陈丹扬广州医科大学附属肿瘤医院胸外科广州510095 广州医科大学附属肿瘤医院肿瘤研究所广州510095

靶向突变的表皮生长因子受体(epidermal growth factor receptor,EGFR)酪氨酸激酶抑制剂(tyrosine kinase inhibitor,TKI)在非小细胞肺癌(non-small cell lung cancer,NSCLC)治疗中显示出越来越重要的作用。然而,不可避免的耐药性的出现极大限制了TKI的临床治疗效果。p21蛋白激活激酶2(p21-activated protein kinase 2,PAK2)是丝/苏氨酸激酶家族的重要成员,在肿瘤发生与发展中发挥重要的作用。本研究拟探讨靶向抑制PAK2逆转非小细胞肺癌吉非替尼耐药的作用及可能机制。首先Western印迹检测发现,相比非小细胞肺癌吉非替尼敏感细胞HCC827,耐药细胞HCC827/GR中PAK2的蛋白质磷酸化水平显著上调(P<0.01),而PAK2总蛋白质水平未见明显变化。采用PAK2抑制剂FRAX597和G5555诱导HCC827/GR细胞,MTS及克隆形成结果显示,抑制剂FRAX597和G5555均能显著增加HCC827/GR细胞对吉非替尼的敏感性(P<0.01)。流式细胞术分析发现,抑制剂FRAX597处理诱导HCC827/GR细胞发生G2/M期阻滞,并增加吉非替尼诱导的HCC827/GR细胞的凋亡以及切割胱天蛋白酶3(cleaved caspase 3)表达(P<0.01)。最后,Western印迹结果显示,抑制剂FRAX597处理能够抑制HCC827/GR细胞BCL-2和CDK4蛋白质表达(P<0.05),上调BAX、p21和p27蛋白质表达(P<0.05)。综上所述,PAK2活化与非小细胞肺癌吉非替尼耐药性密切相关;靶向抑制PAK2活化能够诱导细胞周期阻滞及凋亡,从而提高非小细胞肺癌细胞对吉非替尼的敏感性。

关键词：非小细胞肺癌吉非替尼耐药 p21蛋白激活激酶2 PAK2抑制剂FRAX597

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Conjunctival microbiota variations in a subset of middleaged and elderly individuals from Beijing,China

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International Journal of Ophthalmology(English edition) 2025年第2期18卷 237-243页

作者： Tian-Yao Zhao Yu-chen Wang Ying-Juan Sun Jun-Yue Wang Xiao-dan Jiang Xue-Min Li Ai-Hua Deng Department of Ophthalmology Peking University Third HospitalBeijing Key Laboratory of Restoration of Damaged Ocular NerveBeijing 100191China Fangshan Hospital Beijing University of Chinese Medicine Beijing 102400China CAS Key Laboratory of Pathogenic Microbiology and Immunology Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China State Key Laboratory of Animal Nutrition and Feeding Ministry of Agriculture and Rural Affairs Feed Industry CentreAgricultural UniversityBeijing 100193China

AIM:To isolate and identify the conjunctival microbiota of cataract patients and analyze the associated influencing ***:This study recruited 216 participants(216 eyes)from April 2022 to July *** the condition of no antibiotic use prior to cataract surgery,sterile swabs were used to collect samples from the lower conjunctival *** cultures were then conducted,followed by species identification through 16S rDNA gene *** factors associated with positive or negative bacterial isolation rates were analyzed,including age,gender,meibomian gland dysfunction(MGD),history of hypertension,history of diabetes,history of cancer,history of infectious diseases and the habit of wearing ***:Among the 216 eyes,78 eyes yielded isolates,with an isolation rate of 36.11%,detecting a total of 122 ***-positive rods accounted for 49.18%(60 strains),gram-positive cocci accounted for 45.08%(55 strains),gram-negative bacteria accounted for 4.92%(6 strains),and fungi accounted for 0.82%(1 strain).This study found that the most abundant genera in the conjunctival sac were Corynebacterium(42.62%),Staphylococcus(31.15%),Micrococcus(9.84%),Acinetobacter(4.10%),and Bacillus(3.28%).Furthermore,age(P=0.006),gender(P=0.039),diabetes(P=0.003),history of infectious diseases(P=0.02),and duration of mask replacement(P<0.001)were important factors influencing the positive bacterial culture of the conjunctival *** hypertensive patients exhibited a higher isolation rate of conjunctival bacteria,it did not reach statistical significance,and the history of cancer did not affect the isolation rate of the conjunctival microbial community in cataract patients before ***:Potential changes are observed in the conjunctival microbiota among a sample of middleaged and elderly individuals from Beijing,***,an increased isolation rate of Corynebacterium and Micrococcus is detected,suggesting a possible change in the mic

关键词： conjunctival microbiota 16S rDNA middle-aged and elderly population mask cataract surgery

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Impact of land use change on carbon storage in the middle reaches of the Yellow River,China

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Journal of Arid Land 2025年第2期17卷 167-181页

作者： SHI Xiaoliang ZHANG Jie LIU Simin DING Hao chen Xi WANG Li ZHANG dan College of Geomatics Xi'an University of Science and TechnologyXi'an 710054China China National Forestry-Grassland Development Research Center Beijing 100714China

The implementation of long-term shelterbelt programs in the middle reaches of the Yellow River(MRYR),China not only has improved the overall ecological environment,but also has led to the changes of land use pattern,causing carbon storage ***,the relationship between carbon storage and land use change in the MRYR is not concerned,which results in the uncertainty in the simulation of carbon storage in this *** use changes directly affect the carbon storage capacity of ecosystems,and as an indicator reflecting the overall state of land use,land use degree has an important relationship with carbon *** this study,land use data and the integrated valuation of ecosystem services and trade-offs(InVEST)model were used to assess the trends in land use degree and carbon storage in the MRYR during *** potential impact index and the standard deviation ellipse(SDE)algorithm were applied to quantify and analyze the characteristics of the impact of land use changes on carbon ***,land use transitions that led to carbon storage variations and their spatial variations were *** results showed that:(1)the most significant periods of carbon storage changes and land use transitions were observed during 1990-1995 and 1995-2020,with the most changed areas locating in the east of Fenhe River and in northwestern Henan Province;(2)the positive impact of land use degree on carbon storage may be related to the environmental protection measures implemented along the Yellow River,while the negative impact may be associated with the expansion of construction land in plain areas;and(3)the conversion of other land use types to grassland was the primary factor affecting carbon storage changes during *** future land use planning,attention should be given to the direction of grassland conversion,and focus on reasonably limiting the development of construction *** enhance carbon storage,it will be crucial to increase the area

关键词： carbon storage land use degree integrated valuation of ecosystem services and trade-offs(InVEST)model potential impact standard deviation ellipse(SDE)

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改进的小波阈值函数在超声波无损检测信号中的应用

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闽南师范大学学报（自然科学版） 2014年第2期27卷 52-56页

作者：陈丹阳漳州职业技术学院电子工程系福建漳州363000

针对小波阈值降噪中,软、硬阈值函数的缺点,提出了一种改进的阈值处理函数方法.将新阈值函数用于超声检测信号去噪仿真实验中,仿真结果表明,新阈值函数能够克服软硬阈值的不足,并且选取参数N=30时降噪效果最佳.

关键词：小波分析阈值函数信噪比超声检测

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超声波探伤中探头检测轨迹的一种识别方法

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莆田学院学报 2014年第2期21卷 73-77页

作者：陈丹阳漳州职业技术学院电子工程系福建漳州363000

阐述超声波A扫探伤过程中焊缝缺陷波动态波形特点与探头的检测方式有关,指出识别出探头的检测方式对缺陷的智能识别至关重要。利用Matlab工具箱中的图像处理模块对超声波检测中探头位置识别,并确定出探头的检测轨迹。实践表明,该方法具... 详细信息

阐述超声波A扫探伤过程中焊缝缺陷波动态波形特点与探头的检测方式有关,指出识别出探头的检测方式对缺陷的智能识别至关重要。利用Matlab工具箱中的图像处理模块对超声波检测中探头位置识别,并确定出探头的检测轨迹。实践表明,该方法具有一定的快速性和实用性。

关键词：超声波探头检测轨迹图像处理 Matlab

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桑葚籽毛油超声辅助乙醇脱酸工艺研究

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粮食与油脂 2025年第2期38卷 93-96,111页

作者：陈好杨丹陈晨李波江苏科技大学粮食学院江苏镇江212003 江南大学食品学院江苏无锡214122

采用超声辅助乙醇对桑葚籽油(MSO)进行脱酸处理,以脱酸率为评价指标,在单因素试验的基础上通过正交试验优化脱酸工艺,并与碱炼脱酸对比研究脱酸效果。结果表明:最佳脱酸条件为脱酸温度40℃、超声功率400 W、油溶质量比1∶3、萃取次数3... 详细信息

采用超声辅助乙醇对桑葚籽油(MSO)进行脱酸处理,以脱酸率为评价指标,在单因素试验的基础上通过正交试验优化脱酸工艺,并与碱炼脱酸对比研究脱酸效果。结果表明:最佳脱酸条件为脱酸温度40℃、超声功率400 W、油溶质量比1∶3、萃取次数3。在此条件下,MSO脱酸率可达95.69%。与碱炼脱酸相比,超声辅助乙醇脱酸制得MSO的α-生育酚、β-生育酚和总酚保留率分别提高了21.52%、17.92%和20.93%。

关键词：桑葚籽油超声酸价脱酸

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Spatial Differentiation Characteristics and Driving Factors of Soil Organic Carbon in Various Landscape Complexes

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Huanjing Kexue/Environmental Science 2025年第3期46卷 1679-1689页

作者： Kong, chen-chen Yan, Fang Wang, Wei-Rui Zhang, Shi-Wen Guo, dan-dan Wang, Shan School of Earth and Environment Anhui University of Science & Technology Huainan232001 China Beijing Cultivated Land Construction and Protection Center Beijing100020 China

Investigating the spatial differentiation characteristics of soil organic carbon（SOC）in regional agricultural land and analyzing its driving factors are important for screening auxiliary variables for SOC prediction in agricultural land and the accurate prediction of soil carbon stock. This study considered SOC in different types of agricultural land landscape complexes in Beijing as the research object. The differences in SOC content and its stock in different landscape complexes were explored based on the long-term positional monitoring data on the quality of cultivated land in Beijing and the field sampling and testing data. Utilizing multi-source and open-source data as environmental variables that affected SOC spatial differentiation，we explored the quantitative and spatial relationships between SOC and climate，topography，soil parent material，land use，and biomass factors in different landscape complexes through GeoDetector and geographically weighted regression modeling. Additionally，we constructed a structural equation model to reveal the pathways that influence each driving factor on SOC in terms of direct and indirect effects. Ultimately，the major controlling factors of SOC were identified in the study area. The results showed that：① The mean values of ω（SOC）for various types of landscape complexes in the study area ranged from 6.23 to 28.26 g·kg−1，with a variation coefficient of 3.80% to 33.92%，showing spatial heterogeneity. ② Climate，topography，soil parent material，land type，and biomass factors contributed to SOC at highly significant levels（P © 2025 Science Press. All rights reserved.

关键词： Soil testing

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大鼠脾脏主要结构的发育——从出生至12月龄的形态学定量

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解剖学报 2017年第3期48卷 348-352页

作者：李阳友黄静郭洋项涛吴大鹏陈丹杨正伟川北医学院动物发育研究室四川南充637000 川北医学院实验动物中心四川南充637000 川北医学院形态定量研究室四川南充637000

目的定量研究大鼠从出生至12月龄脾脏主要结构的发育情况。方法从同群正常雄性SD大鼠中,在不同发育阶段(1d和1、3、6、12个月)随机抽选动物,每个年龄组6~7只。取完整的脾脏,切取组织块制作甲基丙烯酸树脂包埋切片,经过碘酸-雪夫试剂和... 详细信息

目的定量研究大鼠从出生至12月龄脾脏主要结构的发育情况。方法从同群正常雄性SD大鼠中,在不同发育阶段(1d和1、3、6、12个月)随机抽选动物,每个年龄组6~7只。取完整的脾脏,切取组织块制作甲基丙烯酸树脂包埋切片,经过碘酸-雪夫试剂和苏木素染色后用体视学方法定量研究脾内主要组织结构的总体积。结果从1d至1月龄,大鼠脾脏体积增长了6.7倍,从1月至3月又增长了2.0倍,从3至12月只增加了39%。脾脏在1日龄已形成动脉周围淋巴鞘,1月形成边缘区,3月形成脾小结,且脾血窦里充满血液。3~12月龄脾内白髓、红髓所占体积比例稳定,分别为23%~27%、67%~70%。结论大鼠脾脏在3月龄前生长快速,发育成熟,之后仍继续生长,但变化不明显。

关键词：脾脏组织学形态学定量体视学大鼠

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TRIM66-HP1γ remodels the chromatin through phase separation

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生物物理学报（英文） 2025年第1期11卷 18-33页

作者： Siyuan Shen Feng chen Yifan Zhang Fudong Li Xuebiao Yao dan Liu Yunyu Shi Liang Zhang Center for Advanced Interdisciplinary Science and Biomedicine of IHM Division of Life Sciences and Medicine University of Science and Technology of China Hefei 230027 China Ministry of Education Key Laboratory for Membraneless Organelles and Cellular Dynamics University of Science and Technology of China Hefei 230027 China Hefei National Research Center for Cross disciplinary Science University of Science and Technology of China Hefei 230027 China

Chromatin contains not only heterochromatin (HC) and euchromatins (EC) but also facultative heterochromatin (fHC), which experience the dynamic remodeling between HCs and ECs by different regulators. The regulation of fHCs involves lots of different cell functions, like genomic stability and gene transcription. Heterochromatin protein 1 (HP1) recognizes methylated H3K9 and reshapes the chromatin into the fHCs through liquid–liquid phase separation (LLPS). Among the three members of the HP1 family, HP1α can condensate by itself and HP1β forms granules with the help of TRIM28, while the HP1γ cannot phase separation alone either and the coordinator is still unclear. So, in this study, we investigated the molecular mechanism of how HP1γ interacts with TRIM66 through PxVxL motif. Based on that, we examined the key regions that controlled the TRIM66-HP1γ co-phase separation behaviors both in vitro and in vivo. Furthermore, we proved that the liquid granules of TRIM66-HP1γ and chromatin highly correlated with H3K9me3 sites, which indicated the relationship with DNA damage response. Finally, combined with our previous study, we proposed the system for how TRIM66 remodeled the chromatin into compressed fHC through the TRIM66-HP1γ-H3K9me3 axis with liquid–liquid phase separation.

关键词： Chromatin TRIM66-HP1γ Facultative heterochromatin Phase separation

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