检索结果-南通市图书馆

在线全文

学校读者我要写书评

暂无评论

棉花独脚金内酯D27基因表达与功能分析

核农学报 2024年第7期38卷 1234-1248页

作者：周强郭栋财蔡永生陈全家曲延英郑凯新疆农业大学农学院新疆乌鲁木齐830052 教育部棉花工程研究中心/作物遗传改良与种质创新重点实验室新疆乌鲁木齐830052

为探究D27基因在棉花纤维及植株发育过程中的生物学功能,本研究通过生物信息学方法鉴定得到棉属中18个D27基因成员,分析其理化性质、结构及进化关系,利用实时荧光定量PCR(qRT-PCR)技术探究了海岛棉纤维中6个GbD27基因的表达特异性,通过... 详细信息

为探究D27基因在棉花纤维及植株发育过程中的生物学功能,本研究通过生物信息学方法鉴定得到棉属中18个D27基因成员,分析其理化性质、结构及进化关系,利用实时荧光定量PCR(qRT-PCR)技术探究了海岛棉纤维中6个GbD27基因的表达特异性,通过同源重组的方法获得了6个陆地棉GhD27基因,构建GhD27基因的病毒诱导的基因沉默(VIGS)载体并转化至棉花,使用qRT-PCR技术检测其沉默效率及独脚金内酯(SLs)处理下的表达模式,并借助酶联免疫吸附(ELISA)技术测定了沉默植株的内源独脚金内酯含量。结果显示,棉花D27基因家族的开放阅读框(ORF)长度为726~816 bp,编码的D27蛋白含241~271个氨基酸;D27蛋白主要由α-螺旋与无规卷曲构成;进化分析结果显示D27基因高度同源。海岛棉材料新海21的GbD27基因在0~35 d的棉纤维中均存在表达,可能对棉纤维发育存在重要调节作用。陆地棉材料新农大棉4号的GhD27基因在叶片和根部均得到有效沉默,表明已获得GhD27基因沉默植株。在陆地棉GhD27基因的沉默植株中,GhD27基因对独脚金内酯处理均有不同程度的响应,内源性独脚金内酯含量与GhD27基因的表达水平有一定关联。本研究为进一步了解棉花D27基因的分子生物学功能奠定了基础。

关键词：棉花 D27 基因克隆表达分析独脚金内酯

维普期刊数据库博看期刊

珍稀濒危植物石山苏铁的SSR引物设计和遗传多样性分析

在线全文

学校读者我要写书评

暂无评论

广西科学 2024年第1期31卷 139-148页

作者：唐健民陈泰国邹蓉盘波韦霄韦发南广西壮族自治区中国科学院广西植物研究所广西桂林541006

揭示珍稀濒危植物石山苏铁(Cycas sexseminifera)种群的遗传多样性水平和遗传分化大小,确定优先保护种群,对石山苏铁的有效保护和管理策略的制定具有重要意义。本研究基于简化基因组序列分析设计Simple Sequence Repeats(SSR)引物,并利... 详细信息

揭示珍稀濒危植物石山苏铁(Cycas sexseminifera)种群的遗传多样性水平和遗传分化大小,确定优先保护种群,对石山苏铁的有效保护和管理策略的制定具有重要意义。本研究基于简化基因组序列分析设计Simple Sequence Repeats(SSR)引物,并利用SSR引物进行遗传多样性检测。实验共获得6对SSR引物,6对引物共检测出37个观测等位基因(N a),其中最小观测等位基因数目为3,最大观测等位基因数目为12,平均每个位点等位基因数目为6.167。位点GZST002、GZST055、GZST065、GZST088是高度多态性位点(PIC>0.5)。石山苏铁各种群期望杂合度(H e)为0.330-0.533,平均值为0.444,表明石山苏铁遗传多样性处于中等水平;7个种群的H e值大小顺序为广西植物研究所引种(ZWS)>崇左广河(GH)>崇左排汝(PR)>隆安陇怀(LH)>崇左中干(ZG)>百色作登(ZD)>隆安新会(xh)。固定系数(F)为-0.161-0.480,平均值为0.074,其中LH、PR、xh 3个野生种群为负值,GH、ZD、ZG 3个野生种群的固定系数则大于0;7个种群的F值大小顺序为百色作登(ZD)>广西植物研究所引种(ZWS)>崇左中干(ZG)>崇左广河(GH)>隆安陇怀(LH)>隆安新会(xh)>崇左排汝(PR)。因此,综合各个遗传指标,石山苏铁需要重点保护和引种的野生种群是遗传多样性相对较高的崇左广河(GH)种群。同时建议开发更多的SSR引物和采集更广范围的石山苏铁野生种群,精准筛选遗传多样性高的优良种群,以利于更全面和准确地评估石山苏铁的遗传多样性水平和濒危机制。

关键词：石山苏铁 SSR分子标记遗传多样性遗传结构保护策略

在线全文

学校读者我要写书评

暂无评论

掺镱大模场光子晶体光纤研究进展(特邀)

中国激光 2024年第1期51卷 280-294页

作者：胡丽丽冯素雅王孟王世凯王璠郭梦婷于春雷陈丹平中国科学院上海光学精密机械研究所上海201800

掺镱大模场光子晶体光纤在高峰值功率超快激光放大器中有着重要的应用价值,其研究得到了广泛关注。首先简要介绍了国内外掺镱大模场光子晶体光纤的研究进展,阐述了掺镱大模场光子晶体光纤的基本设计思路,对比说明了保偏型掺镱光子晶体... 详细信息

掺镱大模场光子晶体光纤在高峰值功率超快激光放大器中有着重要的应用价值,其研究得到了广泛关注。首先简要介绍了国内外掺镱大模场光子晶体光纤的研究进展,阐述了掺镱大模场光子晶体光纤的基本设计思路,对比说明了保偏型掺镱光子晶体光纤的设计制备方法。重点介绍了近十年来中国科学院上海光学精密机械研究所在掺镱大模场光子晶体光纤方面的研究进展。包括掺镱大模场光子晶体光纤的纤芯折射率大小和均匀性控制、光子晶体光纤微结构控制等关键技术。采用自主研制的四种芯径为40~100μm的掺镱大模场光子晶体光纤开展了皮秒脉冲激光放大实验。利用40μm芯径的保偏掺镱光子晶体光纤实现了平均功率为100 W、光束质量因子(M2)小于1.4的稳定输出,偏振消光比为12 dB。利用100μm芯径的保偏掺镱大模场光子晶体光纤实现了M2小于1.5的高光束质量脉冲放大。上述研究为掺镱大模场光子晶体光纤的国产化应用奠定了基础。

关键词：光纤光学掺镱石英玻璃大模场光子晶体光纤皮秒脉冲激光放大光纤激光

Active oxygen radical scavenging ability of water-soluble fullerenols

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Science Bulletin 1997年第9期42卷 748-752页

作者： Sun, DY Zhu, YS Liu, ZY Liu, GZ Guo, xh Zhan, RY Liu, XY State Key Laboratory of Applied Spectroscopy Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun China

THE bioactivity study of C60 and its relative derivatives is of considerable ***60 itself is a non-polar molecule and very difficult to dissolve in common polar solvents,its ap-plication in biochemistry field is greatly *** preparation of some water-solubleC60 derivatives will be *** found that a water-soluble C60 derivative,[HOCO-

关键词： C60 fullerenols active-oxygen-radical ESR spin trapping.

High T_(c) superconductivity in layered hydrides xh_(15) (X = Ca, Sr, Y, La) under high pressures

在线全文

学校读者我要写书评

暂无评论

Frontiers of physics 2022年第6期17卷 107-113页

作者： Yue chen Zhengtao Liu Ziyue Lin Qiwen Jiang Mingyang Du Zihan Zhang Hao Song Hui Xie Tian Cui Defang Duan State Key Laboratory of Superhard Materials College of PhysicsJilin UniversityChangchun 130012China Institute of High Pressure Physics School of Physical Science and TechnologyNingbo UniversityNingbo 315211China

The theoretical predictions and experimental synthesis of H_(3)S and LaH_(10) superconductors with record high superconducting transition temperatures(T_(c))have promoted the hydrogen-based superconducors to be a research hotspot in the field of solid-state ***,we predict an unprecedented layered structure CaH15,with high T_(c) of 189 K at 200 GPa using ab initio *** concerns the novel structure,one layer is made of a hydrogen nonagon,the other layer consists of a Ca atom and six H_(2) molecular units surrounding the Ca *** layered structure was also found in SrH_(15),YH_(15),and LaH_(15) at high pressures,each materials exhibit high T_(c) especially YH_(15) can reach above 200 K at 220 *** represents the second class of layered superhydrides with high value of Tc after pentagraphene like HfH10.

关键词： high pressure hydrides superconductivity ab initio calculation

Spectrum Usage Anomaly Detection from Sub-Sampled Data Stream via Deep Neural Network

在线全文

学校读者我要写书评

暂无评论

Journal of Communications and Information Networks 2023年第1期8卷 13-23页

作者： Han Zhang Jian Yang Junting chen Yue Gao Institute for Communication Systems the University of SurreyGuildford GU27XHUK Mobile Communications Innovation Center China Academy of Information and Communications TechnologyBeijing 100191China Future Network Intelligence Institute(FNii)and School of Science and Engineering the Chinese University of Hong KongShenzhen 518172China School of Computer Science Fudan UniversityShanghai 200433China.Institute for Communication Systemsthe University of SurreyGuildford GU27XHUK

Anomaly detection is an essential part of any practical system in order to remedy any malfunction and accident early to create a secure and robust *** users and malfunctioning cognitive radio(CR)devices may cause severe interference to legitimate ***,there are no effective methods to detect spontaneous and irregular anomaly behaviors in sub-sampling data stream from wideband compressive spectrum sensing as an important function of a CR *** this article,to detect anomaly utilization of spectrum from sub-sampled data stream,a multiple layer perceptron/feed-forward neural network(FFNN)based solution is *** proposed solution would learn the pattern of legitimate and anomalous usages autonomously without expert's *** proposed neural network(NN)framework has also shown benefits such as more than 80%faster detection speed and lower detection error rate.

关键词： compressive sensing(CS) machine learning(ML) multicoset sampling anomaly detection

Association of polymorphisms in the vascular endothelial growth factor gene and its serum levels with diabetic retinopathy in Chinese patients with type 2 diabetes： A cross-sectional study

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Medical Journal 2014年第4期127卷 651-657页

作者： Fan xh Wu QH Li Y Hao YH Ning N Kang Z Cui Y Liu RH Department of Social Medicine School of Health ManagementHarbin Medical University Harbin Heilongjiang 150081 China Second Hospital of Harbin Heilongjiang 150001 China Second Hospital of Harbin Heilongjiang i50001 China Wenhua Community Clinics Qiqihar Heilongjiang 161005 China

Background Vascular endothelial growth factor （VEGF） is a major mediator of angiogenesis,and plays a key role in the pathogenesis of diabetic retinopathy （DR）.This study was designed to identify the possible role of VEGF gene polymorphisms in the development of DR in type 2 diabetic patients in Chinese and clarify the relationship between VEGF serum levels and the risk of *** This cross-sectional study included 1 040 Chinese subjects with type 2 diabetes *** were 372 patients diagnosed with DR in the case group and 668 patients without DR in the control *** from each patient was analyzed for VEGF polymorphisms of-2578A/C （rs699947）,-1154G/A （rs1570360）,-460C/T （rs833061）,＋405C/G （rs2010963）,and ＋936C/T （rs3025039） using MassARRAY compact *** VEGF serum levels were quantified by enzyme-linked immunosorbent assay （ELISA）.Results No evidence of association was observed between-2578 A/C （rs699947）,＋405C/G （rs2010963）,＋936C/T （rs3025039）,and DR risk under stringent Bonferroni＆#39;s ***,VEGF serum levels were significantly higher in DR patients than those of control *** genetic variation of VEGF polymorphisms influenced VEGF serum levels; subjects carrying the VEGF-2578 C/C （rs699947） genotype had greater VEGF serum levels than those carrying the C/A genotype and VEGF serum levels were significantly higher in CC genotype of the ＋405C/G （rs2010963） compared with those of the other *** The data did not suggest significant association between the VEGF polymorphisms and DR risk under stringent Bonferroni＆#39;s ***,our study indicated that DR patients have higher VEGF levels than diabetic patients without retinopathy,and-2578A/C （rs699947） and ＋405C/G （rs2010963） may be important factors in determining serum VEGF levels.

关键词： vascular endothelial growth factor polymorphism genetics diabetic retinopathy

Impact of ionizers on prevention of airborne infection in classroom

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Building Simulation 2023年第5期16卷 749-764页

作者： chen Ren Fariborz Haghighat Zhuangbo Feng Prashant Kumar Shi-Jie Cao School of Architecture Southeast University2 SipailouNanjing 210096China Energy and Environment Group Department of BuildingCivil and Environmental EngineeringConcordia UniversityMontrealH3G 1M8Canada Global Centre for Clean Air Research(GCARE) School of SustainabilityCivil&Environmental EngineeringFaculty of Engineering&Physical SciencesUniversity of SurreyGuildford GU27XHSurreyUK Institute for Sustainability University of SurreyGuildford GU27XHSurreyUK

Infectious diseases(e.g.,coronavirus disease 2019)dramatically impact human life,economy and social *** the low-cost and energy-saving approaches is essential in removing infectious virus particles from indoors,such as in *** application of air purification devices,such as negative ion generators(ionizers),gains popularity because of the favorable removal capacity for particles and the low operation ***,small and portable ionizers have potential disadvantages in the removal efficiency owing to the limited horizontal diffusion of negative *** study aims to investigate the layout strategy(number and location)of ionizers based on the energy-efficient natural ventilation in the classroom to improve removal efficiency(negative ions to particles)and decrease infection *** infected students were considered in the *** simulations of negative ion and particle concentrations were performed and validated by the *** showed that as the number of ionizers was 4 and 5,the removal performance was largely improved by combining ionizer with natural *** with the scenario without an ionizer,the scenario with 5 ionizers largely increased the average removal efficiency from around 20%to 85%and decreased the average infection risk by 23%.The setup with 5 ionizers placed upstream of the classroom was determined as the optimal layout strategy,particularly when the location and number of the infected students were *** work can provide a guideline for applying ionizers to public buildings when natural ventilation is used.

关键词： classroom infection risk ionizer negative ions removal efficiency

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

田耀洲教授辨治慢性萎缩性胃炎经验采撷

光明中医 2024年第8期39卷 1526-1529页

作者：陈颖田耀洲林琳丁金荣南京中医药大学附属中西医结合医院消化科江苏南京210028 南京中医药大学第三临床医学院江苏南京210046 无锡市中医院消化科江苏无锡214071

慢性萎缩性胃炎(CAG)是消化系统常见病,具有病情反复、缠绵难愈的特点。病理主要以胃腺体的萎缩、伴或不伴有肠上皮化生或异型增生为特征。肠上皮化生和异型增生属于癌前病变,与胃癌的发生关系密切,积极控制CAG患者的临床症状,延缓其病... 详细信息

慢性萎缩性胃炎(CAG)是消化系统常见病,具有病情反复、缠绵难愈的特点。病理主要以胃腺体的萎缩、伴或不伴有肠上皮化生或异型增生为特征。肠上皮化生和异型增生属于癌前病变,与胃癌的发生关系密切,积极控制CAG患者的临床症状,延缓其病理进展或逆转其病理变化,有利于改善患者的生活质量、减轻经济负担及预防胃癌发生。田耀洲教授认为此病属本虚标实,脾胃虚弱是本,气滞、湿郁、瘀血为标,虚、滞、湿、瘀相互胶结推动着疾病的进展,在辨证上推崇微观与宏观相结合,用药上推崇中西医结合,并擅用经方与药对,精准辨治与用药。田教授开创的自拟方剂芪芝方,疗效显著,可有效改善脾胃湿热型CAG的临床症状。今将田教授辨治CAG之经验及自拟方剂分享给大家,以期为临床辨治CAG提供参考借鉴。

关键词：慢性萎缩性胃炎脾胃湿热证名医经验