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Electrochemically modified piezoelectric crystal biosensor for detection of Staphylococcus aureus

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Chinese Journal of Chemistry 1996年第3期14卷 222-227页

作者： Si, SH chen, jh He, FJ Nie, LH Yao, SZ HUNAN UNIV NEW MAT RES INSTCHANGSHA 410082HUNANPEOPLES R CHINA

Electropolymerized o-phenylenediamine firm is used as a functional coating for the immobilization of anti-S. aureus antibody on the surface of a gold-plated piezoelectric crystal, and this piezoelectric immunosensor is applied to detect S. aureus. The frequency shift (Delta F = F-20s - F-380s, Hz) between the frequency at the 20th second (after the addition of sample, F-20s) and that at 380 seconds later (F-380s) was introduced to construct a calibration graph, and shortening of assay time was achieved. The S. aureus concentrations in the range of 10(5)-10(9) cells/mL can be detected by this system.

关键词： piezoelectric biosensor detection of S-aureus electrochemical modification

Up-regulation of PIK3CA promotes metastasis in gastric carcinoma

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World Journal of Gastroenterology 2010年第39期16卷 4986-4991页

作者： Liu JF Zhou XK chen jh Yi G chen HG Ba MC Lin SQ Qi YC. Institute of Cancer Research Affiliated Tumor Hospital of Guangzhou Medical College Guangzhou 510095 Guangdong Province China. zxkstar@***

AIM:To explore expressions of PIK3CA in the progression of gastric cancer from primary to metastasis and its effects on activation of phosphatidylinositol 3-kinase(PI3K)/Akt ***:mRNA and protein levels of PIK3CA were assessed,respectively,by real-time quantitative polymerase chain reaction and immunohistochemistry in specimens of normal gastric mucosa,primary foci and lymph node and distant metastasis of gastric *** and phosphorylated Akt protein were also examined by Western blotting in these tissues,in order to analyze the effect of PIK3CA expression level changes on the activation of PI3K/Akt signaling ***:PIK3CA mRNA in lymph node metastasis were approximately 5 and 2 folds higher,respectively,than that in the corresponding normal gastric mucosa and primary gastric cancer tissues(P<0.05),while no statistical significance was found compared with distant ***,PIK3CA protein expression was discovered in 7(35%)specimens of 20 primary foci vs 10(67%)of 15 of lymph node metastasis or 11(61%)of 18 of distant metastasis(35%vs 67%,P=0.015;35%vs 61%,P=0.044).With the increased level of PIK3CA expression,the total Akt protein expression remained almost unchanged,but p-Akt protein was upregulated ***:Increased expression of PIK3CA is expected to be a promising indicator of metastasis in gastric ***-regulation of PIK3CA may promote the metastasis of gastric cancer through aberrant activation of PI3K/Akt signaling.

关键词： PIK3CA Phosphatidylinositol 3-kinase/Akt pathway Metastasis Gastric cancer Akt

Quality control measures for lowering the seroconversion rate of hemodialysis patients with hepatitis B or C virus

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Hepatobiliary & Pancreatic Diseases International 2012年第3期11卷 302-306页

作者： Jing Yuan,Yi Yang, Fei Han, Ping Zhang, Xiao-Ying Du, Hua Jiang and Jiang-Hua chen The Kidney Disease Center, First Affiliated Hospital, Zhejiang University School of Medicine (Yuan J, Yang Y, Han F, Zhang P, Du XY, Jiang H and chen jh) and Hemodialysis Quality Control Center of Zhejiang Province (Yuan J, Zhang P, Du XY and chen jh), Hangzhou 310003, China The Kidney Disease Center First Affiliated Hospital Zhejiang University School of Medicine Hangzhou 310003 China Hemodialysis Quality Control Center of Zhejiang Province Hangzhou 310003 China

BACKGROUND: Hemodialysis (HD) patients are at high risk of infection by hepatitis B virus (HBV) or hepatitis C virus (HCV). The present study was designed to determine the impact of quality control measures on the prevention of transmission of blood-borne viruses. METHODS: A total of 6182 adult maintenance HD patients from all HD units in Zhejiang Province were recruited on January 1, 2007. The baseline demographic and clinical characteristics were recorded and all patients were followed up until death or survival at 4 years later. The Quality Control Standards of Hemodialysis were gradually implemented in HD units. The HBV or HCV seroconversion rates of the recruited patients were calculated and compared every year during the observation period. RESULTS: The prevalence of HBV was 8.3% at the beginning of the study, and 6.6% for HCV. With the implementation of the HD quality control measures, the HBV seroconversion rate tended to decrease year by year (χ 2 =6.620, P=0.085), and the HCV seroconversion rate decreased significantly (χ 2 =10.41, P=0.015). Compared with the data in 2007, the HBV seroconversion rate (χ 2 =4.204, P=0.040, relative risk ratio 0.393, 95% CI 0.156-0.991) and the HCV seroconversion rate (χ 2 =7.373, P=0.007, relative risk ratio 0.386, 95% CI 0.189-0.787) decreased significantly in 2010. CONCLUSION: Quality control measures for HD decreased the seroconversion rates of HBV or HCV in HD patients, showing that updated quality control measures reduce the risk for transmission of blood-borne viruses in the HD population.

关键词： hemodialysis quality control measures infection hepatitis B virus hepatitis C virus

A PERTURBATION PROCEDURE FOR LIMIT CYCLE DETERMINATION IN THREE-DIMENSIONAL NONLINEAR AUTONOMOUS DYNAMICAL SYSTEMS

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A PERTURBATION PROCEDURE FOR LIMIT CYCLE DETERMINATION IN TH...

基于血氧水平依赖性的功能磁共振成像联合弥散张量成像技术纵向观察视觉通路病变中皮质功能和白质纤维结构的动态变化

庆祝中国力学学会成立50周年暨中国力学学会学术大会’2007

作者： SHEN jh chen SH~(2)) (Department of Applied Mechanics and Engineering,Sun Yat-sen University,Guangzhou,510275,P.R.China)

By introducing a new parametric transformation and suitable nonlinear frequency expansion in the perturbation procedure of the modified Lindstedt-Poincare method,analytical approximation for limit cycle in a three-dim... 详细信息

关键词： analytical approximation limit cycle nonlinear autonomous systems modified Lindstedt-Poincare method

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中国组织工程研究与临床康复 2007年第31期11卷 6218-6223页

作者：肖慧陈自谦钱根年倪萍叶友强陈锦华解放军南京军区福州总医院医学影像科福建省福州市350025

目的:联合应用血氧依赖功能磁共振成像和弥散张量成像技术纵向观察视觉通路病变的皮质功能和白质纤维结构的动态变化,探讨病变后恢复期脑功能和结构重组的特点。方法:病变组患者为2006-01/09解放军南京军区福州总医院收治,因视觉通路病... 详细信息

目的:联合应用血氧依赖功能磁共振成像和弥散张量成像技术纵向观察视觉通路病变的皮质功能和白质纤维结构的动态变化,探讨病变后恢复期脑功能和结构重组的特点。方法:病变组患者为2006-01/09解放军南京军区福州总医院收治,因视觉通路病变致单侧或双侧视觉障碍患者8例,采用美国GE公司Signa Excite HD 1.5T双梯度16通道磁共振成像系统,在病变早期和恢复期分别进行血氧依赖功能磁共振成像和弥散张量成像检查,对比治疗前后双眼刺激下脑激活区的体积以及视放射解剖结构、局部各向异性值,并与对照组12名正常被试比较。结果:20例受试者均完成测试进入结果分析。①血氧依赖功能磁共振成像结果:正常被试采用黑白棋盘格刺激明显激活双侧枕叶距状回,平均激活皮质体素数为(9.12±2.47)万;病变组患者双眼刺激视皮质激活体积较对照组比较明显减少(P<0.05),治疗后复查,刺激的皮质激活像素数增多,激活区扩大。②弥散张量成像三维纤维束重建:对照组双侧视放射均能追踪至皮质下,12例被试视放射平均部分各项异性值为0.39±0.13。病变组中4例视觉通路病变患者视放射显示完整,与对照组间比较无明显差异,治疗前后纵向观察亦无显著变化;4例枕叶视中枢病变患者患侧视放射纤维部分中断、纤细,并可见移位,部分各项异性值均明显下降,治疗后视放射纤维束有一定程度恢复,部分各项异性值增加。结论:血氧水平依赖功能磁共振成像联合弥散张量成像从皮质功能和纤维解剖方面诠释了病变的发展及康复过程,可帮助了解视觉通路病变后皮质功能区的重组。

关键词：功能磁共振成像弥散张量成像视觉通路康复

Enhancing metformin-induced tumor metabolism destruction by glucose oxidase for triple-combination therapy

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Journal of Pharmaceutical Analysis 2024年第3期14卷 321-334页

作者： Rangrang Fan Linrui Cai Hao Liu Hongxu chen Caili chen Gang Guo Jianguo Xu Department of Neurosurgery and Institute of Neurosurgery Cancer Center and State Key Laboratory of BiotherapyWest China HospitalSichuan UniversityChengdu610041China NMPA Key Laboratory for Technical Research on Drug Products in Vitro and in Vivo Correlation Key Laboratory of Birth Defects and Related Diseases of Women and Children of MOENational Drug Clinical-Trial InstitutionWest China Second HospitalSichuan UniversityChengdu610041China Department of Immunology School of Basic Medical SciencesXinxiang Medical UniversityXinxiangHenan453000China

Despite decades of laboratory and clinical trials,breast cancer remains the main cause of cancer-related disease burden in *** the metabolism destruction effect of metformin(Met)and cancer cell starvation induced by glucose oxidase(GOx),after their efficient delivery to tumor sites,GOx and Met may consume a large amount of glucose and produce sufficient hydrogen peroxide in ***,a pH-responsive epigallocatechin gallate(EGCG)-conjugated low-molecular-weight chitosan(LC-EGCG,LE)nanoparticle(Met–GOx/Fe@LE NPs)was *** coordination between iron ions(Fe3+)and EGCG in this nanoplatform can enhance the efficacy of chemodynamic therapy via the Fenton ***–GOx/Fe@LE NPs allow GOx to retain its enzymatic activity while simultaneously improving its ***,this pH-responsive nanoplatform presents controllable drug release *** in vivo biodistribution study showed that the intracranial accumulation of GOx delivered by this nanoplatform was 3.6-fold higher than that of the free *** in vivo anticancer results indicated that this metabolism destruction/starvation/chemodynamic triple-combination therapy could induce increased apoptosis/death of tumor cells and reduce their *** triple-combination therapy approach is promising for efficient and targeted cancer treatment.

关键词： Metformin Glucose oxidase Metabolism disruption Tumor starvation Combination cancer therapy

急性胆囊炎腹腔镜胆囊切除术中转开腹相关风险因素的分析

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中国普通外科杂志 2016年第2期25卷 286-290页

作者：冯一浮莫经刚陈建辉温州医科大学第一临床学院浙江温州325600 浙江省台州市中心医院肝胆外科浙江台州318000

目的:探讨致急性胆囊炎患者腹腔镜胆囊切除术(LC)中转开腹的影响因素。方法:回顾分析2011年1月—2015年6温州医科大学附属浙江省台州市中心医院及台州医院1 161例急性胆囊炎行LC患者的临床资料,选择中转开腹的56例患者为研究组,随机选... 详细信息

目的:探讨致急性胆囊炎患者腹腔镜胆囊切除术(LC)中转开腹的影响因素。方法:回顾分析2011年1月—2015年6温州医科大学附属浙江省台州市中心医院及台州医院1 161例急性胆囊炎行LC患者的临床资料,选择中转开腹的56例患者为研究组,随机选取78例顺利完成LC术患者为对照组,分析中转开腹的相关风险因素。结果:单因素分析表明,纤维蛋白原、白蛋白、糖尿病、胆囊壁厚度、是否胆囊坏疽、术中出血量均与LC中转开腹有关(均P<0.05)。Logistic回归分析结果表明,低白蛋白(OR=1.258)、术中出血量(OR=0.988)是LC中转开腹独立危险因素(均P<0.05)。结论:低白蛋白、纤维蛋白原、糖尿病、胆囊坏疽及胆囊壁增厚及术中出血量是LC中转开腹的危险因素。

关键词：胆囊炎,急性胆囊切除术,腹腔镜危险因素

Positive effects of biochar application and Rhizophagus irregularis inoculation on mycorrhizal colonization,rice seedlings and phosphorus cycling in paddy soils

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Pedosphere 2024年第2期34卷 361-373页

作者： Yixuan chen Zhonghua WEN Jun MENG Zunqi LIU Jialong WEI Xiyu LIU Ziyi GE Wanning DAI Li LIN Wenfu chen National Biochar Institute Shenyang Agricultural UniversityShenyang 110866China Key Laboratory of Biochar and Soil Improvement Ministry of Agriculture and Rural AffairsShenyang 110866China

Phosphorus(P)is an essential element for plant growth but is often limiting in ecosystems;therefore,improving the P fertilizer use efficiency is *** and arbuscular mycorrhizal fungi(AMF)may enhance P cycling in paddy soils that contain high content of total P but low content of available P(AP).In this study,the effects of biochar addition and Rhizophagus irregularis inoculation on the organic and inorganic P contents and phosphatase activities in paddy soils,rice seedling growth,and AMF colonization were *** with no biochar addition,biochar addition enhanced the percentage of spore germination at day 7,hyphal length,most probable number,and mycorrhizal colonization rate of *** by 32%,662%,70%,and 28%on average,*** and *** altered soil P cycling and *** and ***,either individually or in combination,increased soil AP content by 2%–48%.Rice seedlings treated with biochar and *** produced greater biomass,improved root morphology,and increased nutrient uptake compared with those of the control without biochar and *** results suggest that combined application of biochar and *** is beneficial to rice cultivation in paddy soils with high content of total P but low content of AP.

关键词： arbuscular mycorrhizal fungi available P phosphorus fraction rice husk biochar seedling growth spore germination

Juvenile hormone induces reproduction via miR-1175-3p in the red imported fire ant, Solenopsis invicta

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Insect Science 2024年第2期31卷 371-386页

作者： Jie chen Ziying Guan Yunjie Ma Qingxing Shi Ting chen Muhammad Irfan WarisLihua Lyu Lihua Lyu Yongyue Lu Guojun Qi Institute of Plant Protection Guangdong Academy of Agricultural SciencesKey Laboratory of Green Prevention and Control on Fruits and Vegetables in South China Ministry of Agriculture and Rural AffairsGuangdong Provincial Key Laboratory of High Technology for Plant ProtectionGuangzhouChina Department of Entomology College of Plant ProtectionSouth China Agricultural UniversityGuangzhouChina

Juvenile hormone(jh)acts in the regulation of caste differentiation between queens and workers(i.e.,with or without reproductive capacity)during vitellin synthesis and oogenesis in social ***,the regulatory mechanisms have not yet been ***,we identified a highly expressed microRNA(miRNA),miR-1175-3p,in the red imported fire ant,Solenopsis *** found that miR-1175-3p is prominently present in the fat bodies and ovaries of ***,miR-1175-3p interacts with its target gene,broad-complex core(Br-C),in the fat *** utilizing miR-1175-3p agomir,we successfully suppressed the expression of the Br-C protein in queens,resulting in reduced vitellogenin expression,fewer eggs,and poorly developed ***,decreasing miR-1175-3p levels led to the increased expression of Br-C and vitellogenin in workers,triggering the“re-development”of the ***,when queens were fed with jh,the expression of miR-1175-3p decreased,whereas the expression of vitellogenin-2 and vitellogenin-3 ***,the suppression of fertility in queens caused by treatment with agomir miR-1175-3p was completely rescued by the increased vitellogenin expression induced by being fed with *** results suggest the critical role of miR-1175-3p in jh-regulated reproduction,shedding light on the molecular mechanism underlying miRNA-mediated fecundity in social insects and providing a novel strategy for managing ***.

关键词： juvenile hormone miR-1175-3p red imported fire ant reproduction social insects vitellogenin