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类风湿关节炎患者外周血单个核细胞System Xc-/GSH/GPX4铁死亡通路的表达及其对炎症因子分泌的影响

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安徽医科大学学报 2024年第1期59卷 64-70页

作者：刘灿马武开陈昌明安阳蒋总黄海贵州医科大学医学检验学院临床生物化学与分子生物学教研室贵阳550004 贵州中医药大学第二附属医院风湿免疫科贵阳550004 贵州医科大学附属医院临床检验中心贵阳550004

目的探讨类风湿关节炎(RA)患者外周血单个核细胞(PBMC)胱氨酸/谷氨酸逆转运体(System Xc-)/谷胱甘肽(GSH)/谷胱甘肽过氧化物酶4(GPX4)铁死亡通路中相关基因与蛋白的表达及其对炎症因子分泌的影响。方法纳入RA患者及同期健康体检者各30例... 详细信息

目的探讨类风湿关节炎(RA)患者外周血单个核细胞(PBMC)胱氨酸/谷氨酸逆转运体(System Xc-)/谷胱甘肽(GSH)/谷胱甘肽过氧化物酶4(GPX4)铁死亡通路中相关基因与蛋白的表达及其对炎症因子分泌的影响。方法纳入RA患者及同期健康体检者各30例,采用Ficoll-hypaque密度梯度离心法分离PBMC,将细胞分为健康对照、RA、铁死亡抑制剂、铁死亡诱导剂组。采用细胞计数试剂盒8(CCK-8)检测细胞活力,流式细胞术FerroOrange荧光探针检测细胞内Fe 2+相对荧光强度、DHE荧光探针检测细胞内活性氧(ROS)阳性细胞比例,蛋白免疫印迹及实时荧光定量PCR(qPCR)检测核转录因子红系2相关因子(Nrf2)、溶质载体家族7成员11(SLC7A11)、GPX4蛋白与mRNA的表达,流式细胞术检测细胞上清液中肿瘤坏死因子α(TNF-α)、白细胞介素(IL)-1、IL-6的含量。结果与健康对照组比较,RA组PBMC内Fe 2+浓度和ROS水平升高,Nrf2、SLC7A11、GPX4蛋白和mRNA的表达降低,PBMC上清液中TNF-α、IL-1、IL-6的含量升高,差异均有统计学意义。与RA组比较,铁死亡抑制剂组PBMC内Fe 2+浓度和ROS水平降低,SLC7A11、GPX4蛋白和mRNA的表达升高,Nrf2蛋白的表达升高,PBMC上清液中IL-6的含量降低、TNF-α的含量升高,差异均有统计学意义。与RA组比较,铁死亡诱导剂组PBMC内ROS水平升高,SLC7A11蛋白和mRNA的表达降低,Nrf2蛋白的表达降低、GPX4蛋白的表达升高,PBMC上清液中TNF-α、IL-1的含量升高,差异有统计学意义。与铁死亡诱导剂组比较,RA患者PBMC细胞活力降低,差异有统计学意义(P<0.0001)。结论RA患者PBMC中存在铁死亡,抑制或诱导RA患者PBMC铁死亡,将抑制或促进炎性因子分泌。抑制RA患者PBMC铁死亡可能有助于治疗RA。

关键词：类风湿关节炎外周血单个核细胞铁死亡 System Xc-/GSH/GPX4

Identification and Characterization of a LEA-like Gene, CaMF5,Specifically Expressed in the Anthers of Male-fertile Capsicum annuum

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Horticultural Plant Journal 2020年第1期6卷 39-48页

作者： Yudan Wang Guoju chen Jianjun Lei Bihao Cao changming chen Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in South China Ministry of AgricultureCollege of HorticultureSouth China Agricultural University

To improve the understanding of molecular mechanisms of anther and/or pollen development in Chili pepper, in the present study, fulllength cDNA and DNA sequences of the pollen development-related gene CaMF5 were obtained from the anthers of a Capsicum annuum nuclear male-fertile line. Sequence analysis indicated that the full length of CaMF5 was 747 bp, containing a maximum opening reading frame of 447 *** acid sequence alignment and phylogenetic analysis revealed that CaMF5 shared approximately 37%–77% homology with a series of uncharacterized or hypothetical proteins and late embryogenesis abundant(LEA) proteins from other plants. However, no LEA structural domain was detected in CaMF5, which indicated that it might be a new type of LEA gene. CaMF5 was only expressed in flower buds at stages 7 and 8 and in open flowers of the male-fertile line, whereas it exhibited no expression in any examined organs of the male-sterile line. In addition, CaMF5 showed the highest transcript abundance in the anthers of the male-fertile line, with no expression being detected in any other examined organs, such as the sepals, petals, pistils, roots, stems, or leaves. Taken together, our results suggest that CaMF5 is an anther-specific gene that might encode a new type of LEA protein related to anther and/or pollen development in C. annuum.

关键词： Capsicum annuum CaMF5 Anther-specific gene Late embryogenesis abundant Expression analysis

云南红河流域土地利用变化的地形梯度效应及其功能分区

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云南大学学报（自然科学版） 2024年第2期46卷 276-287页

作者：杨李玲和阳一丹唐红陈昌明和克俭云南大学国际河流与生态安全研究院云南昆明650500 云南大学地球科学学院云南昆明650500

明确土地利用情况及其地形梯度分布格局,并进行功能分区,是云南红河流域土地资源可持续利用和土地利用规划优化调整的基础.基于RS和GIS技术,使用地形分布指数、地形位指数方法系统分析了2000、2010年和2020年云南红河流域土地利用变化... 详细信息

明确土地利用情况及其地形梯度分布格局,并进行功能分区,是云南红河流域土地资源可持续利用和土地利用规划优化调整的基础.基于RS和GIS技术,使用地形分布指数、地形位指数方法系统分析了2000、2010年和2020年云南红河流域土地利用变化在高程、坡度和地形位梯度上的时空分异特征,并基于分析结果开展了云南红河流域土地利用功能区划分.结果表明:(1)林地(56.46%)和耕地(29.34%)是云南红河流域主要的土地利用类型,其次是草地(6.93%)和灌木地(6.17%),而湿地、水体和人造地表所占面积比例均不到1%.2000—2020年云南红河流域耕地和人造地表面积扩张明显,而林地持续减少,存在林地被侵占的现象;(2)云南红河流域土地利用类型分布呈现明显的地形梯度特征.耕地、人造地表受到高地形位开发难度的限制,优势位处于中低地形位的河谷、山腰区域,湿地、水体高度汇集在低地形位,草地、灌木地在中低地形位较为集中,林地则广泛分布于中高地形位;(3)基于土地利用地形分异规律,将云南红河流域划分为4个土地利用功能区,即河谷集约发展区、陡坡综合治理区、高山潜在开发区和山顶生态调控区.

关键词：云南红河流域土地利用地形梯度分布指数地形位功能分区

电子鼻的深度神经网络算法实现可燃气体检测的研究

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传感技术学报 2024年第9期37卷 1518-1524页

作者：房瑞山薛莹莹陈畅明向奕王平万浩浙江大学生仪学院生物传感器国家专业实验室浙江杭州310027 浙江大学生仪学院生物医学工程教育部重点实验室浙江杭州310027

提出了一种将格拉姆角场与深度残差卷积神经网络相结合的电子鼻气体识别算法,可以提高燃气中常见混合危险气体的识别准确度。通过对电子鼻的气体样本数据都进行格拉姆角场(Graham Angle Field,GAF)变换,使得上位机接收到的二维传感器响... 详细信息

提出了一种将格拉姆角场与深度残差卷积神经网络相结合的电子鼻气体识别算法,可以提高燃气中常见混合危险气体的识别准确度。通过对电子鼻的气体样本数据都进行格拉姆角场(Graham Angle Field,GAF)变换,使得上位机接收到的二维传感器响应数据,经过升维后成为可以输入到卷积神经网络(Convolutional Neural Network,CNN)中的三维数据形式,从而发挥了CNN特征提取能力强、模型收敛快和识别准确率高的优势。实验结果表明,该算法在干扰气体存在下的情况下,对CO和CH4的检测准确率分别达到93.04%和92.43%,通过与多种主流算法进行对比和分析,表明该算法具有抗干扰性高、检测准确率高的优点,为实际环境中干扰气体存在时的可燃气体高可靠性和特异性检测提供了一种具有良好应用前景的智能识别算法。

关键词：电子鼻气体识别模式识别卷积神经网络

Facial Expression Enhances Emotion Perception Compared to Vocal Prosody： Behavioral and fMRI Studies

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Neuroscience Bulletin 2018年第5期34卷 801-815页

作者： Heming Zhang Xuhai chen Shengdong chen Yansong Li changming chen Quanshan Long Jiajin Yuan Key Laboratory of Cognition and Personality of the Ministry of Education School of Psychology Southwest University Chongqing 400715 China Key Laboratory of Behavior and Cognitive Psychology in Shaanxi Province School of Psychology Shaanxi Normal University Xi'an 710062 China Key Laboratory of Modem Teaching Technology of the Ministry of Education Shaanxi Normal University Xi'an 710062 China Department of Psychology School of Social and Behavioral Sciences Nanjing University Nanjing 210023 China School of Educational Sciences Xinyang Normal University Xinyang 464000 China

Facial and vocal expressions are essential modalities mediating the perception of emotion and social communication. Nonetheless, currently little is known about how emotion perception and its neural substrates differ across facial expression and vocal prosody. To clarify this issue, functional MRI scans were acquired in Study 1, in which participants were asked to discriminate the valence of emotional expression （angry, happy or neutral） from facial, vocal, or bimodal stimuli. In Study 2, we used an affective priming task （unimodal materials as primers and bimodal materials as target） and participants were asked to rate the intensity, valence, and arousal of the targets. Study 1 showed higher accuracy and shorter response latencies in the facial than in the vocal modality for a happy expression. Whole-brain analysis showed enhanced activation during facial compared to vocal emotions in the inferior temporal-occipital regions. Region of interest analysis showed a higher percentage signal change for facial than for vocal anger in the superior temporal sulcus. Study 2 showed that facial relative to vocal priming of anger had a greater influence on perceived emotion for bimodal targets, irrespective of the target valence. These findings suggest that facial expression is associated with enhanced emotion perception compared to equivalent vocal prosodies.

关键词： fMRI Emotion perception Facial expres-sion Vocal prosody Modality

Germplasm Diversity of Chinese Kale in China

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Horticultural Plant Journal 2017年第3期3卷 101-104页

作者： LEI Jianjun chen Guoju chen changming CAO Bihao Key Laboratory of Biology Innovation and Utilization for Germplasm Resources in Horticultural Crops in Southern China College of HorticultureSouth China Agricultural University

Chinese kale is an important vegetable crop in China, especially in South China where the majority of varieties are grown. It originated in South China and spread throughout Southeast Asia thereafter. Chinese kale can be classified into two types according to whether the petals are white or yellow. There are also three main cultivated types based on the edible organs, including the stalk and leaf type, the stalk type,and the leaf type. Two primary types have also been defined based on stalk color, including green stalks and red stalks. They are also cultivated based on the type of stalk, including main stalk and lateral stalk types. Significant differences have also been observed in glucosinolate content among the varieties, and a 40-fold difference in neoindle-3-methyl glucosinolate was detected across the cultivars.

关键词： Chinese kale Brassica alboglabra Bailey diversity

丁香止痛膏联合三阶梯止痛药治疗癌痛的随机交叉对照研究

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中华中医药杂志 2024年第1期39卷 514-518页

作者：王曼胡凯文王静卫月陈昌明任卫国田桢刘传波北京市丰台区中医医院(北京市丰台区南苑医院) 北京100076 北京中医药大学东方医院北京100078 陕西科技大学西安710021

目的:采用前瞻性、随机交叉对照、双盲设计探究丁香止痛膏治疗癌痛的疗效。方法:选择癌痛患者58例进行随机分组,不同组别按顺序使用试验组方案(三阶梯止痛药+丁香止痛膏)和对照组方案(三阶梯止痛药+安慰剂)进行干预,对比治疗前后数字评... 详细信息

目的:采用前瞻性、随机交叉对照、双盲设计探究丁香止痛膏治疗癌痛的疗效。方法:选择癌痛患者58例进行随机分组,不同组别按顺序使用试验组方案(三阶梯止痛药+丁香止痛膏)和对照组方案(三阶梯止痛药+安慰剂)进行干预,对比治疗前后数字评分量表(NRS)评分、生活质量(QOL)评分、血清学指标和满意度评分。结果:两组中度疼痛患者NRS评分比较,试验组方案干预后的NRS评分低于对照组方案(P<0.05);两组中度疼痛患者QOL评分比较,试验组方案干预后的QOL评分高于对照组方案(P<0.05);两组患者血清学指标比较差异均无统计学意义;两组满意度评分比较,患者对试验组方案治疗更满意(P<0.05)。结论:温阳理论指导下的丁香止痛膏外敷能改善中度癌痛患者NRS评分和生活质量。

关键词：癌痛丁香止痛膏交叉试验温通中药贴敷

Cross-cascaded AWG-based wavelength selective switching integrated module using polymer optical waveguide circuits

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Frontiers of Optoelectronics 2016年第3期9卷 428-435页

作者： changming chen Darning ZHANG State Key Laboratory on Integrated Optoelectronics College of Electronic Science and EngineeringJilin University

100-GHz cross-cascaded arrayed waveguide gratings（AWGs）-based wavelength selective optical switching optical cross-connects（OXCs） modules with Mach-Zehnder interferometer（MZI） thermo-optic（TO）variable optical attenuator（VOA） arrays and optical truetime-delay（TTD） line arrays is successfully designed and fabricated using polymer photonic lightwave *** fluorinated photopolymer and grafting modified organic-inorganic hybrid material were synthesized as the waveguide core and cladding,*** one-chip transmission loss is6dB and the crosstalk is less than30dB for the transverse-magnetic（TM） *** actual maximum modulation depths of different thermo-optic switches are similar,15.5 dB with 1.9 V *** maximum power consumption of a single switch is less than 10 *** delay time basic increments are measured from 140 to 20 *** novel module is flexible and scalable for the dense wavelength division multiplexing network.

关键词： polymer waveguides photosensitive materials integrated optics devices photonics integrated circuits

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从废弃光伏组件中回收银的研究现状

贵金属 2024年第S01期45卷 97-103页

作者：田相亮管有祥陈昌明法正福王志雄彭春生永兴贵研资源有限公司湖南郴州423399 贵研铂业股份有限公司昆明650106

随着光伏电站的大力开发,白银的需求持续增长,同时,太阳能光伏板更新迭代加快伴随大量废弃光伏组件产生。废弃光伏组件中含有大量银、铝、硅等高价值组分,回收废弃光伏组件不仅可以减轻环境污染,还能对我国白银及铝、硅等资源形成补充... 详细信息

随着光伏电站的大力开发,白银的需求持续增长,同时,太阳能光伏板更新迭代加快伴随大量废弃光伏组件产生。废弃光伏组件中含有大量银、铝、硅等高价值组分,回收废弃光伏组件不仅可以减轻环境污染,还能对我国白银及铝、硅等资源形成补充再返回到光伏制造业,形成循环利用。本文通过对国内外相关文献进行分析,总结了各种物理预处理方法、化学分离提纯方法,对比了不同方法的回收效果,分析了现有技术的优势及存在问题,为今后从废弃光伏组件中回收银的发展提供参考和建议。

关键词：废弃物光伏组件高价值组分银研究现状