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Is the time right for systematically serotyping SARS-related coronaviruses?

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hLife 2024年第1期2卷 4-5页

作者： chee wah tan Lin-Fa Wang Department of Microbiology and Immunology Yong Loo Lin School of MedicineNational University of SingaporeSingapore CitySingapore Duke-NUS Medical School Singapore CitySingapore

IS THERE A NEED FOR SEROTYPING?The emergence and rapid selection of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)immune escape variants have exerted significant challenges in many different ways,from public health response to vaccine boosting strategy and monitoring of herd immunity against future variants or related severe acute respiratory syndrome(SARS)related coronaviruses(SARSr-CoVs).The Omicron sub-variants,which differ substantially from the pre-Omicron variants,are circulating worldwide and continuing to evolve extensively through mutations and/or recombination predominantly in the spike protein,mainly due to immune-selective *** their phylogenetically close relationship to ancestral SARS-CoV-2,antigenically,Omicron variants are very different from the original *** is therefore very natural to raise the question whether we need a better way to classify and differentiate those CoVs which are genetically very similar,but antigenically quite *** of viruses is a common practice for many known virus groups,most notably for human papillomavirus(HPV)and dengue virus(DENV)[1,2].The concept of CoV serotypes was not new and has been applied to study the antigenicity of feline CoVs[3].The possibility of establishing distinctive serotypes for the SARS-CoV-2 virus was first raised by Simon-Loriere and Schwartz in 2022[4].Serotyping SARS-CoV-2 is expected to help more scientifically define the differences between variants and facilitate more targeted diagnostics,therapeutics,and vaccine development.

关键词： immunity SARS establishing

An unusual COVID-19 case with over four months of viral shedding in the presence of low neutralizing antibodies: a case report

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The Journal of Biomedical Research 2020年第6期34卷 470-474页

作者： Wei Chen Zhiliang Hu Changhua Yi Yun Chi Qingfang Xiong chee wah tan Yongxiang Yi Lin-Fa Wang Clinical Research Center the Second Hospital of NanjingNanjing University of Chinese MedicineNanjingJiangsu 210003China Nanjing infectious Disease Center the Second Hospital of NanjingNanjing University of Chinese MedicineNanjingJiangsu 210003China Center for Global Health School of Public HealthNanjing Medical UniversityNanjingJiangsu 211166China Programme in Emerging Infectious Diseases Duke-NUS Medical SchoolSingapore 169857Singapore

The ongoing coronavirus disease 2019(COVID-19)pandemic is a global public health crisis,causing social and economic disasters in many *** China,two-consecutive negative results of nucleic acid tests for SARS-CoV-2 from the respiratory samples are required to end the quarantine of COVID-19 ***,clinicians face a dilemma in case of patients with long-term viral *** report described an unusual COVID-19 case who had persistent viral RNA positivity for more than 4 months after initial illness in the presence of low neutralizing antibodies,but without prolonged clinical *** anti-viral drug treatments had no impact and there was no evidence of *** the patient was self-quarantined at home,no infection occurred to the three family members living with her for 15 to 19 *** viral culture in BSL-3 laboratory on the 102nd day after symptom onset was *** the 129th day on,8 continuous nucleic acid tests of sputum samples showed negative *** patient was discharged on 137th days since symptom *** conclusion,viral RNA shedding in the sputum of the COVID-19 patient may last over 4 *** no evidence shows the existence of infectious virus,two-consecutive negative nucleic acid tests may not be the prerequisite for ending quarantine of COVID-19 patients with prolonged viral shedding.

关键词： COVID-19 SARS-CoV-2 viral shedding neutralizing antibodies quarantine

Distinctive serotypes of SARS-related coronaviruses defined by convalescent sera from unvaccinated individuals

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hLife 2023年第1期1卷 26-34页

作者： chee wah tan Feng Zhu Wan Ni Chia #Barnaby EYoung Aileen Ying Yan Yeoh Thomas Althaus chee Fu Yung Wee chee Yap Beng Lee Lim Mark I-C Chen Jinyan Zhang Yun Yan Mah Eric Voiglio Alex Sigal Jianxin Huo Shengli Xu Yee Joo tan Kong-Peng Lam David Lye Lin-Fa Wang Programme in Emerging Infectious Diseases Duke-NUS Medical SchoolSingapore National Center of Infectious Diseases Singapore tan Tock Seng Hospital Singapore Lee Kong Chian School of Medicine Nanyang Technology UniversitySingapore Centre Scientifique de Monaco Monaco Direction de I’Action Sanitaire de Monaco Monaco Infectious Disease Service Department of PediatricsKK Women’s and Children’s HospitalSingapore Duke-NUS Medical School Singapore African Health Research Institute DurbanSouth Africa Singapore Immunology Network Agency for ScienceTechnology and ResearchSingapore Department of Physiology Yong Loo Lin School of MedicineNational University of SingaporeSingapore Department of Microbiology and Immunology Yong Loo Lin School of MedicineNational University of SingaporeSingapore Infectious Diseases Translational Research Programme National University of SingaporeSingapore 14School of Biological SciencesNanyang Technological UniversitySingapore Singhealth Duke-NUS Global Health Institute Singapore

Multiple Omicron sub-lineages have emerged,with Omicron XBB and XBB.1.5 subvariants becoming the dominant variants globally at the time of this *** key feature of new variants is their ability to escape humoral immunity despite the fact that there are limited genetic changes from their preceding *** raises the question of whether Omicron should be regarded as a separate serotype from viruses serologically clustered with the ancestral severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)***,we present cross-neutralization data based on a pseudovirus neutralization test using convalescent sera from naïve individuals who had recovered from primary infection by SARS-CoV-1 and SARS-CoV-2 strains/variants including the ancestral virus and variants Beta,Delta,Omicron BA.1,Omicron BA.2 and Omicron *** results revealed no significant cross-neutralization in any of the three-way testing for SARS-CoV-1,ancestral SARS-CoV-2 and SARS-CoV-2 Omicron *** data argue for the assignment of three distinct serotypes for the currently known human-infecting SARS-related coronaviruses.

关键词： SARS-CoV-2 SARS-CoV-1 serotype Omicron primary infection convalescent sera

Pan-Sarbecovirus Neutralizing Antibodies in BNT162b2-lmmunized SARS-CoV-1 Survivors

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四川生理科学杂志 2021年第8期43卷 1438-1438页

作者： chee-wah tan 不详

Emerging severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)variants of concern pose a challenge to the effectiveness of current vaccines.A vaccine that could prevent infection caused by known and future variants of concern as well as infection with pre-emergent sarbecoviruses(i.e.,those with potential to cause disease in humans in the future)would be *** we provide data showing that potent cross-clade pan-sarbecovirus neutralizing antibodies are induced in survivors of severe acute respiratory syndrome coronavirus 1(SARS-CoV-1)infection who have been immunized with the BNT162b2 messenger RNA(mRNA)vaccine.

关键词： vaccine vivo respiratory

The host-targeting compound peruvoside has a broad-spectrum antiviral activity against positive-sense RNA viruses

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Acta Pharmaceutica Sinica B 2023年第5期13卷 2039-2055页

作者： Kan Xing Wu Thinesshwary Yogarajah Marcus Wing Choy Loe Parveen Kaur Regina Ching Hua Lee chee Keng Mok Yi Hao Wong Patchara Phuektes Li Sze Yeo Vincent T.K.Chow Yong wah tan Justin Jang Hann Chu Department of Microbiology and Immunology Yong Loo Lin School of MedicineNational University Health SystemNational University of SingaporeSingapore 117597Singapore Department of Pathobiology Faculty of Veterinary MedicineKhonkaen UniversityKhonkaen 40002Thailand Infectious Disease Programme Yong Loo Lin School of MedicineNational University of SingaporeSingapore 117597Singapore Institute of Molecular and Cell Biology Agency for ScienceTechnology and Research(A∗STAR)Singapore 138648Singapore Lee Kong Chian School of Medicine Nanyang Technological UniversitySingapore 308232Singapore NUSMed Biosafety Level 3 Core Facility Yong Loo Lin School of MedicineNational University of SingaporeSingapore 117599Singapore

Positive-sense RNA viruses modify intracellular calcium stores,endoplasmic reticulum and Golgi apparatus(Golgi)to generate membranous replication organelles known as viral *** factories provide a conducive and substantial enclave for essential virus replication via concentrating necessary cellular factors and viral proteins in ***,we identified the vital role of a broadspectrum antiviral,peruvoside in limiting the formation of viral ***,we revealed the pleiotropic cellular effect of Src and PLC kinase signaling via cyclin-dependent kinase 1 signaling leads to Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1(GBF1)phosphorylation and Golgi vesiculation by peruvoside *** ramification of GBF1 phosphorylation fosters GBF1 deprivation consequentially activating downstream antiviral signaling by dampening viral factories *** investigation showed signaling of ERK1/2 pathway via cyclin-dependent kinase 1 activation leading to GBF1 phosphorylation at Threonine 1337(T1337).We also showed 100%of protection in peruvoside-treated mouse model with a significant reduction in viral titre and without measurable cytotoxicity in *** findings highlight the importance of dissecting the broad-spectrum antiviral therapeutics mechanism and pave the way for consideration of peruvoside,host-directed antivirals for positive-sense RNA virus-mediated disease,in the interim where no vaccine is available.

关键词： Positive-sense RNA viruses Peruvoside Antiviral CDKI signaling Viral factories Calcium efflux Broad-spectrum Golgi apparatus

Microwave heating as a universal method to transform confined molecules into armchair graphene nanoribbons

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Nano Research 2023年第7期16卷 10644-10651页

作者： Haoyuan Zhang Yingzhi Chen Kunpeng tang Ziheng Lin Xuan Li Hongwei Zhang Yifan Zhang Chi Ho Wong Chi wah Leung chee Leung Mak Yuan Hu Weili Cui Kecheng Cao Lei Shi State Key Laboratory of Optoelectronic Materials and Technologies Nanotechnology Research CenterGuangdong Province Key Lab of Display Material and TechnologySchool of Materials Science and EngineeringSun Yat-sen UniversityGuangzhou 510275China Huzhou Key Laboratory of Environmental Functional Materials and Pollution Control School of EngineeringHuzhou UniversityHuzhou 313000China Department of Industrial and Systems Engineering Research Institute for Advanced ManufacturingThe Hong Kong Polytechnic UniversityHong Kong 999077China Department of Applied Physics The Hong Kong Polytechnic UniversityHong Kong 999077China Shanghai Key Laboratory of High-resolution Electron Microscopy ShanghaiTech UniversityShanghai 201210China

Armchair graphene nanoribbons(AGNRs)with sub-nanometer width are potential materials for the fabrication of novel nanodevices thanks to their moderate direct band *** are usually synthesized by polymerizing precursor molecules on substrate ***,it is time-consuming and not suitable for large-scale *** can also be grown by transforming precursor molecules inside single-walled carbon nanotubes(SWCNTs)via furnace annealing,but the obtained AGNRs are normally *** this work,microwave heating is applied for transforming precursor molecules into *** fast heating process allows synthesizing the AGNRs in *** different molecules were successfully transformed into AGNRs,suggesting that it is a universal *** importantly,as demonstrated by Raman spectroscopy,aberrationcorrected high-resolution transmission electron microscopy and theoretical calculations,less twisted AGNRs are synthesized by the microwave heating than the furnace *** results reveal a route for rapid production of AGNRs in large scale,which would benefit future applications in novel AGNRs-based semiconductor devices.

关键词： armchair graphene nanoribbons(AGNRs) microwave heating single-walled carbon nanotubes(SWCNTs) Raman spectroscopy

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基于高频电阻抗信号的结构损伤监测

浙江大学学报（工学版） 2007年第1期41卷 6-11页

作者：严蔚陈伟球林志华蔡金标浙江大学土木工程学系浙江杭州310027 香港城市大学建筑系

给出了利用高频电阻抗信号进行结构损伤识别的精确定量分析方法.考察压电片-黏结层-主体结构组成的一个耦合结构系统,主体结构采用一维模型并只考虑轴向振动,引入剪滞模型来描述压电片与主体结构之间的剪力传递,考虑压电片的动力效应,... 详细信息

给出了利用高频电阻抗信号进行结构损伤识别的精确定量分析方法.考察压电片-黏结层-主体结构组成的一个耦合结构系统,主体结构采用一维模型并只考虑轴向振动,引入剪滞模型来描述压电片与主体结构之间的剪力传递,考虑压电片的动力效应,采用传递矩阵法导出了这一耦合结构的系统方程,得到了压电片电导纳的解析表达式.通过算例验证了所给出方法的正确性和有效性.计算结果表明,弱界面对电阻抗信号有较大的影响,利用电导纳的虚部可以清楚地检测到界面的弱黏结;采用电阻抗技术能够很好地识别结构局部的细小损伤.

关键词：损伤识别电阻抗法剪滞模型传递矩阵法

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一种经济、快速的成形血泵叶轮的方法

一种经济、快速的成形血泵叶轮的方法

Interventional radiology in living donor liver transplant

2005年中国机械工程学会年会

作者：冯超查建中 Chua chee Kai Wong Yew wah 北京交通大学机电学院北京交通大学机电学院 School of Mechanical and Production Engineering Nanyang Technological University Singapore School of Mechanical and Production Engineering Nanyang Technological University Singapore

轴向血泵叶轮具有旋转的流线曲面,其叶片厚度不超过1.5mm,叶轮轴直径小于16mm。对于这种构件采用机械加工的方式很难一次成形出所有特征。快速成形工艺如立体光刻工艺（Stereolithigraphy apparatus）、熔融沉积成形工艺（Fused deposit... 详细信息

轴向血泵叶轮具有旋转的流线曲面,其叶片厚度不超过1.5mm,叶轮轴直径小于16mm。对于这种构件采用机械加工的方式很难一次成形出所有特征。快速成形工艺如立体光刻工艺（Stereolithigraphy apparatus）、熔融沉积成形工艺（Fused deposition modeling）也不能完整成形出小而薄的叶片。本文提出并且研究了一种经济而快速的方法,可以很好地成形出小叶轮。这种方法先用三维打印工艺（3D Printer process）成形出血泵叶轮的原型,然后以原型为母型制作硅橡胶模具,第三步通过硅橡胶模具注塑聚亚安酯件。聚亚安酯件质地坚硬,可以用于血泵的体外流体实验。为了评价本方法的成形精度,将成形的原型通过三维扫描技术得到表面点云,将扫描得到的点云与叶轮原始的CAD模型比较,得到物理原型和电子模型之间的偏差,从而全面评价出成形的质量。

关键词：心脏血泵快速成形/快速模具计算机辅助校验三维扫描

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World Journal of Gastroenterology 2014年第20期20卷 6221-6225页

作者： Yu-Fan Cheng Hsin-You Ou Chun-Yen Yu Leo Leung-Chit Tsang Tung-Liang Huang Tai-Yi Chen Hsien-Wen Hsu Allan M Concerjero Chih-Chi Wang Shih-Ho Wang Tsan-Shiun Lin Yueh-Wei Liu chee-Chien Yong Yu-Hung Lin Chih-Che Lin King-wah Chiu Bruno Jawan Hock-Liew Eng Chao-Long Chen Department of Diagnostic Radiology Kaohsiung Chang Gung Memorial Hospital Chang Gung University College of Medicine Department of Surgery Kaohsiung Chang Gung Memorial Hospital Chang Gung University College of Medicine Division of Hepatogastroenterology Kaohsiung Chang Gung Memorial Hospital Chang Gung University College of Medicine Department of Anesthesiology Kaohsiung Chang Gung Memorial Hospital Chang Gung University College of Medicine Department of Pathology Kaohsiung Chang Gung Memorial Hospital Chang Gung University College of Medicine

The shortage of deceased donor liver grafts led to the use of living donor liver transplant(LDLT).Patients who un-dergo LDLT have a higher risk of complications than those who undergo deceased donor liver transplantation(LT).Interventional radiology has acquired a key role in every LT program by treating the majority of vascular and nonvascular post-transplant complications,improving graft and patient survival and avoiding,in the majority of cases,surgical revision and/or *** aim of this paper is to review indications,diagnostic modalities,technical considerations,achievements and potential complications of interventional radiology procedures after LDLT.

关键词： Portal vein Hepatic artery Hepatic vein Bile duct Living donor liver transplantation Liver transplant